1352572241471 205839e681c91839b3bb1d7435d4b458

High Throughput Sequencing and Microarray Facility

Princeton Township, New Jersey, US

The Lewis-Sigler Institute for Integrative Genomics houses the Sequencing Core Facility which is operated in conjunction with the Microarray Core Facility and provides researchers with access to state of the art, high-throughput sequencing technologies. The staff provide consultation on experimental design, library preparation, and data analysis. The Sequencing Core Facility works closely with Bioinformatics staff in the Center for Quantitative Biology to provide researchers with computing power and consulting services to analyze sequencing data.

The facility currently houses a state-of-the-art Illumina HiSeq 2000 and two Ion Torrent Personal Genome Machines.

High Throughput Sequencing and Microarray Facility has not listed any services.

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Affymetrix RNA microarray
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Agilent RNA microarray
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Agilent Bioanalyzer
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Illumina Sequencing
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Illumina HiSeq 2000
The core will accept nucleic acid sample and perform library construction (for an additional fee) or we will accept pre-made libraries.

Sample Preparation Guidelines
1. Genomic DNA prep: DNA should be intact and high quality, gel photo is required. A minimum of 0.5 µg with a concentration of 50-500 ng/µl... Show more »

Illumina HiSeq 2000
The core will accept nucleic acid sample and perform library construction (for an additional fee) or we will accept pre-made libraries.

Sample Preparation Guidelines
1. Genomic DNA prep: DNA should be intact and high quality, gel photo is required. A minimum of 0.5 µg with a concentration of 50-500 ng/µl in TE (If you used Nanodrop to check the concentration, please provide a concentration in the range of 100-500 ng/µl, because Nanodrop tends to over-estimate DNA concentration).
2. ChIP DNA prep: 10 ng ChIP enriched DNA in 30 µl water in a low retention tube (we recommend verifying with QPCR if possible).
3. Pre-made Libraries: A minimum of 20 µl of 5ng/ul pre-made library in EB (Tris-Cl 10mM, pH 8.5) must be provided in a low retention tube. Bioanalyzer QC of library size is highly recommended for each library (before pooling multiplexed libraries).
4. Custom Primer: Custom sequencing primer concentration should be at 100 µM, with a minimum volume of 20 µl.

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Illumina NGS Library Preparation
Starting at $320.00 per sample
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Ion Torrent Sequencing
Ion Torrent Next Generation Sequencing
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DNA Sequencing
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RNA Microarray Platforms
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Biology
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Equipment
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Next Generation Sequencing (NGS)
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RNA Microarray
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DNA
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RNA
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DNA Library Construction Services
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Biochemistry & Molecular Biology
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Nucleic Acid Services
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