1326141776000

High Throughput Gene Expression/Biomarker Core Laboratory

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Worcester, Massachusetts, US

About High Throughput Gene Expression/Biomarker Core Laboratory

The High Throughput Gene Expression/Biomarker Core Laboratory run by Drs. Tanriverdi and Freedman provides High Throughput/Rapid Gene Expression and Complete miRNA profiling analysis by using gold standard qRT-PCR with the combination of custom Integrated Fluidic Circuit (IFC) technology from Fluidigm (South... Show more »

The High Throughput Gene Expression/Biomarker Core Laboratory run by Drs. Tanriverdi and Freedman provides High Throughput/Rapid Gene Expression and Complete miRNA profiling analysis by using gold standard qRT-PCR with the combination of custom Integrated Fluidic Circuit (IFC) technology from Fluidigm (South San Francisco, CA).

Also, high-throughput SNP and multi-plate immunoassay services are available. The core lab is located in a modular class 10,000 clean room.

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Our Services (23)


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Biomarker Analysis

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ELISA Gene expression profiling

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ELISA

Enzyme-linked immunosorbent assay
Price on request

High-Throughput Immunoassay Analysis:
The lab can run up to 7 different ELISA assays from the same set of samples at once robotically. This avoids freeze-thawing of samples as the assays can be run simultaneously by utilizing scheduling software integrated into the liquid handling robot software and a liquid sensing Varispan pipetting arm.

High-Throughput Immunoassay Analysis:
The lab can run up to 7 different ELISA assays from the same set of samples at once robotically. This avoids freeze-thawing of samples as the assays can be run simultaneously by utilizing scheduling software integrated into the liquid handling robot software and a liquid sensing Varispan pipetting arm.

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DNA Library Preparation

Price on request

qRT-PCR analyses are performed using Integrated Fluidic Circuit (IFC) chips (Dynamic Arrays 1, from Fluidigm) using a Liquid Handling Robot (Janus Automated Workstation2). Dynamic Arrays are able to conduct 9,216 Real-Time PCR reactions extremely efficiently and rapidly (within 3 hours). The primary advantage of this platform and... Show more »

qRT-PCR analyses are performed using Integrated Fluidic Circuit (IFC) chips (Dynamic Arrays 1, from Fluidigm) using a Liquid Handling Robot (Janus Automated Workstation2). Dynamic Arrays are able to conduct 9,216 Real-Time PCR reactions extremely efficiently and rapidly (within 3 hours). The primary advantage of this platform and technology is that it can complete thousands of reactions with minimal pipetting steps and much smaller volumes of reagents. This significantly saves time and experimental costs.

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Fluidigm Access Array

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SNP Genotyping

Single Nucleotide Polymorphism Genotyping
Price on request

Ninety-six samples can be analyzed for 96 SNPs in under 3 hours. Standard TaqMan SNP assays can be used. Users may also choose to submit their SNPs for SNPtype assay design service provided by Fluidigm. This service lowers the assay cost for SNP analysis (approx. $300.00 for TaqMan vs. $50.00 for Fluidigm). Please contact with us for more detailed information.

Ninety-six samples can be analyzed for 96 SNPs in under 3 hours. Standard TaqMan SNP assays can be used. Users may also choose to submit their SNPs for SNPtype assay design service provided by Fluidigm. This service lowers the assay cost for SNP analysis (approx. $300.00 for TaqMan vs. $50.00 for Fluidigm). Please contact with us for more detailed information.

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Single Cell Gene Expression Analysis

Price on request

Ninety-six single cells can be analyzed for hundreds of gene expression assays. Single cells can be collected many ways such as flow cytometer cell sorting, micro injection or Laser micro dissection. RNA isolation is not required. Technique can be used small numbers of cells (up to 1,000 cells) too. Standard TaqMan SNP assays or... Show more »

Ninety-six single cells can be analyzed for hundreds of gene expression assays. Single cells can be collected many ways such as flow cytometer cell sorting, micro injection or Laser micro dissection. RNA isolation is not required. Technique can be used small numbers of cells (up to 1,000 cells) too. Standard TaqMan SNP assays or your own primers can be used. Users may also choose to submit their genes for DELTAgene assay design service provided by Fluidigm. This service lowers the assay cost for gene expression analysis (approx. $155.00 for TaqMan vs. $30-75.00 for Fluidigm).

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microRNA Taqman Low Density Array

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qPCR

Quantitative PCR
Price on request

High-Throughput Gene Expression Analysis:
Ninety-six samples can be analyzed for 96 gene expression assays in under 3 hours. Standard TaqMan assays or your own primers can be used. Users may also choose to submit their genes for DELTAgene assay design service provided by Fluidigm. This service lowers the assay cost for gene... Show more »

High-Throughput Gene Expression Analysis:
Ninety-six samples can be analyzed for 96 gene expression assays in under 3 hours. Standard TaqMan assays or your own primers can be used. Users may also choose to submit their genes for DELTAgene assay design service provided by Fluidigm. This service lowers the assay cost for gene expression analysis (approx. $155.00 for TaqMan vs. $30-75.00 for Fluidigm). Please contact with us for more detailed information.

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MicroRNA (miRNA) Quantification

Quantitative PCR microRNA quantitation
Price on request

High-Throughput miRNA Profiling:
Ninety-six samples can be analyzed for 768 miRNA assays in one business day. TaqMan miRNA assays are provided by core. Human and Rodent miRNA assay libraries are available.

High-Throughput miRNA Profiling:
Ninety-six samples can be analyzed for 768 miRNA assays in one business day. TaqMan miRNA assays are provided by core. Human and Rodent miRNA assay libraries are available.

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Biology

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Nucleic Acid Services

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Equipment

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Bioanalysis

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Genotyping and Gene Expression Assays

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Protein Quantification

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Protein Services

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Pharmacology & Toxicology

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DNA Library Services

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DNA Libraries Services

DNA Libraries Services

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RNA Services

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Drug Discovery & Development

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Drug Discovery & Development Services

Drug Discovery & Development Services

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PCR

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Biochemistry & Molecular Biology

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Biomarkers

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DNA Services

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Kahraman Tanriverdi

Co-Director

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