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Hadassah BrainLabs

Jerusalem, IL

Building on the expertise of neuroscientists at Hadassah - Hebrew University Medical Center, Hadassah BrainLabs is uniquely positioned to serve researchers from academia and the biomedical and pharmaceutical industries. Hadassah BrainLabs is recognized as a National Knowledge Center for Research on Brain Diseases by the Israel Ministry of Science

Our disease models and assay panels help researchers to study novel compounds, discover novel indications for existing compounds and phenotypically characterize genetically modified mice. Researchers may choose from and combine a range of experimental options into a customized package:

DISEASE MODELS (Genetic or Interventional)
For Alzheimer’s disease, multiple sclerosis, ALS, prion diseases, depression, schizophrenia, autism, anxiety and other neuropsychiatric disorders. Customized and composite models can be developed on request.

OUTCOME MEASURES
• Behavioral... Show more »

Building on the expertise of neuroscientists at Hadassah - Hebrew University Medical Center, Hadassah BrainLabs is uniquely positioned to serve researchers from academia and the biomedical and pharmaceutical industries. Hadassah BrainLabs is recognized as a National Knowledge Center for Research on Brain Diseases by the Israel Ministry of Science

Our disease models and assay panels help researchers to study novel compounds, discover novel indications for existing compounds and phenotypically characterize genetically modified mice. Researchers may choose from and combine a range of experimental options into a customized package:

DISEASE MODELS (Genetic or Interventional)
For Alzheimer’s disease, multiple sclerosis, ALS, prion diseases, depression, schizophrenia, autism, anxiety and other neuropsychiatric disorders. Customized and composite models can be developed on request.

OUTCOME MEASURES
• Behavioral analysis of motor, cognitive, emotional and social domains in rodent models using state of the art computerized tracking systems. Animals are housed in a specific pathogen free (SPF) colony (AAALC certified).
• Biochemical and immunological markers
• Brain imaging using Micro-PET

Leading members of the Hadassah BrainLabs team include Prof. Bernard Lerer (Director); Prof. Hanna Rosenmann (Scientific Manager); Prof Tamir Ben-Hur (Associate Director); Prof Talma Brenner (Myasthenia Gravis, Neuroinflammation); Prof. Ruth Gabizon (Prion Diseases, Biochemistry); Prof. Dimitrios Karussis (Multiple Sclerosis; Mesenchymal Stem Cells; Neuroimmunology); Dr Tzuri Lifschytz (Disease Models of Psychiatric Disorders, Animal Behavior Analysis; Dr Amit Lotan (Disease Models of Psychiatric Disorders, Animal Behavior Analysis); Dr Iris Lavon (Genotyping); Prof Eyal Mishani (Brain Imaging, Micro-PET)

The added value of Hadassah BrainLabs:

• Unique expertise of clinician-scientists bridges the basic-clinical divide to help researchers who are interested in developing new diagnostic approaches and novel therapies.
• Capacity to take studies from experimental design to patent application and academic publication.
• Practical assistance with regulatory processes including ethical approval, design of experiments to meet regulatory requirements and comprehensive reports.
• Large projects as well as small, focused experiments undertaken

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Alzheimer's Disease Animal Models
Price on request

GENETIC MODELS

Amyloid (APPswe,PSEN1De9) tg- mice
Amyloid (APPswe,PSEN1De9) tg-mice present amyloid plaques by 9 months of age and develop cognitive deficits at 13 months.

Amyloid (5XFAD) tg-mice
[5XFAD (B6.Cg-Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/J)] tg-mice present intracellular and extracellular amyloid plaques... Show more »

GENETIC MODELS

Amyloid (APPswe,PSEN1De9) tg- mice
Amyloid (APPswe,PSEN1De9) tg-mice present amyloid plaques by 9 months of age and develop cognitive deficits at 13 months.

Amyloid (5XFAD) tg-mice
[5XFAD (B6.Cg-Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/J)] tg-mice present intracellular and extracellular amyloid plaques at 2 months, develop cognitive impairments at 4-5 months and neuronal death at 9 months.

PHARMACOLOGICAL MODELS

ICV Aβ -induced amnesia
I.C.V. administration of Aβ peptide in mice induces cognitive impairment and neuronal loss

Acetylcholine receptor antagonist-induced amnesia
Amnesia is induced in mice within 1 hour following i.p. injection of acetylcholine receptor antagonists (scopolamine, mecamylamine).

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Mouse
Tauopathy Animal Models
Price on request

Tangle (htauP301S/K257T)-tg mice
Neurofibrillary tangle (NFT)-tg mice (htauP301S/ K257T) model, generated by us, presents NFT pathology with astrogliosis in the brain, memory deficits and long-term potentiation deficits by 7 months of age. This model serves in addition to Alzheimer’s disease (a secondary tauopathy) also as a... Show more »

Tangle (htauP301S/K257T)-tg mice
Neurofibrillary tangle (NFT)-tg mice (htauP301S/ K257T) model, generated by us, presents NFT pathology with astrogliosis in the brain, memory deficits and long-term potentiation deficits by 7 months of age. This model serves in addition to Alzheimer’s disease (a secondary tauopathy) also as a model for primary tauopathies (frontotemporal dementia, Pick’s disease).

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Mouse
Multiple Sclerosis Animal Models
Price on request

EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS (EAE)

Acute EAE
Lewis Rats are immunized with spinal cord homogenate to induce an acute, transient paralytic disease, manifesting with neuroinflammation, but limited demyelination and axonal injury.
Lewis Rats are immunized with spinal cord homogenate to induce an acute, transient... Show more »

EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS (EAE)

Acute EAE
Lewis Rats are immunized with spinal cord homogenate to induce an acute, transient paralytic disease, manifesting with neuroinflammation, but limited demyelination and axonal injury.
Lewis Rats are immunized with spinal cord homogenate to induce an acute, transient paralytic disease, manifesting with neuroinflammation, but limited demyelination and axonal injury.

Chronic EAE
C57BL/6 mice are immunized with MOG35-55 peptide, to induce adaptive immunity – mediated demyelination and axonal injury, manifesting with acute paralytic disease and chronic neurologic residua. The neuroinflammatory process is characterized by perivascular and meningeal infiltrates of macrophages / microglia and T cells.

Relapsing EAE

SJL mice are immunized with PLP peptide, to induce an autoimmune response. Lymph node cells are further exposed to the autoantigen in vitro and then injected to another set of SJL mice for passive induction (“transfer”) of EAE. The clinical course, neuro-inflammation, tissue injury and T cell responses are measured, as in other EAE models.

Relapsing-Progressive EAE
Biozzi AB/H mice are immunized with spinal cord homogenate to induce a chronic relapsing EAE. This model manifests with initial relapsing-remitting course, followed by a relapsing progressive disease, with pathological elements of demyelination, remyelination, axonal loss, gliosis and chronic neuroinflammation. These are reminiscent of progressive multiple sclerosis.

TOXIC MODEL

Cuprizone model of toxic demyelination

C57BL/6 mice are fed with cuprizone for 6-10 weeks to induced widespread demyelination, especially in the splenium of the corpus callosum. Upon discontinuation of cuprizone, a concerted process of remyelination is evident

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Mouse
Rat
EAE Animal Model
Experimental Autoimmune Encephalomyelitis Animal Model
Price on request

Acute EAE
Lewis Rats are immunized with spinal cord homogenate to induce an acute, transient paralytic disease, manifesting with neuroinflammation, but limited demyelination and axonal injury.
Lewis Rats are immunized with spinal cord homogenate to induce an acute, transient paralytic disease, manifesting with neuroinflammation,... Show more »

Acute EAE
Lewis Rats are immunized with spinal cord homogenate to induce an acute, transient paralytic disease, manifesting with neuroinflammation, but limited demyelination and axonal injury.
Lewis Rats are immunized with spinal cord homogenate to induce an acute, transient paralytic disease, manifesting with neuroinflammation, but limited demyelination and axonal injury.

Chronic EAE
C57BL/6 mice are immunized with MOG35-55 peptide, to induce adaptive immunity – mediated demyelination and axonal injury, manifesting with acute paralytic disease and chronic neurologic residua. The neuroinflammatory process is characterized by perivascular and meningeal infiltrates of macrophages / microglia and T cells.

The EAE score is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Neurological panel (EAE, ALS, prion, neurological severity, myasthenia gravis scores).

This score evaluates the severity of symptoms in an animal model of induced experimental autoimmune encephalomyelitis. Animals are scored daily for neurological symptoms according to the EAE clinical severity scale: 0 = asymptomatic; 1 = partial loss of tail tonicity; 2 = tail paralysis; 3 = hind limb weakness; 4 = hind limb paralysis; 5 = 4-limb paralysis; 6 = death. [This test is applicable to rats and mice].

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Mouse
Rat
Myasthenia Gravis Animal Model
Price on request

Experimental autoimmune myasthenia gravis (EAMG)
The disease is induced in mice or rats by immunization with purified Torpedo acetylcholine receptor (AChR). Most animals develop the myasthenic typical muscle weakness 4-6 weeks later.

Experimental autoimmune myasthenia gravis (EAMG)
The disease is induced in mice or rats by immunization with purified Torpedo acetylcholine receptor (AChR). Most animals develop the myasthenic typical muscle weakness 4-6 weeks later.

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Rat
ALS Animal Models
Amyotrophic Lateral Sclerosis Animal Models
Price on request

ALS (SOD1-G93A) tg-mice
B6SJL-TgN[SOD1-G93A]1Gur mice29, which show Amyotrophic Lateral Sclerosis (ALS) related clinical symptoms starting at about 14 -15 weeks of age, presenting as weakness progressing into disability to ambulate, with loss of lumbar motor neurons, and death at 18-20 weeks.

ALS (SOD1-G93A) tg-mice
B6SJL-TgN[SOD1-G93A]1Gur mice29, which show Amyotrophic Lateral Sclerosis (ALS) related clinical symptoms starting at about 14 -15 weeks of age, presenting as weakness progressing into disability to ambulate, with loss of lumbar motor neurons, and death at 18-20 weeks.

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Mouse
Depression Animal Models
Price on request

SURGICAL MODELS

Olfactory bulbectomy
This model mimics behavioral, physiological and neurochemical features of depression, such as deficits in learning and memory, reduced food motivated behavior and libido, and stress hyper-responsiveness. Bulbectomised animals show impairment of cognitive function. This animal model... Show more »

SURGICAL MODELS

Olfactory bulbectomy
This model mimics behavioral, physiological and neurochemical features of depression, such as deficits in learning and memory, reduced food motivated behavior and libido, and stress hyper-responsiveness. Bulbectomised animals show impairment of cognitive function. This animal model resembles the agitated depression phenomena found in humans. This model is applicable for both rats and mice.

White matter lesions
Bilateral common artery stenosis is a model for white matter ischemic vascular damage, by causing cerebral hypoperfusion via partial bilateral common carotid artery occluasion. It is implemented by twining a 0.18 mm diameter steel coil around the two CCA’s (common carotid arteries). The surgical procedure, apart from causing white matter diffused damage, also demonstrated to exert cognitive impairments in the Y maze (working memory, reference memory) and the 8 arm maize (working memory). This model is applicable only to mice,

ENVIRONMENTAL MODELS

Chronic mild stress (CMS)
A depressive like behavioral state is induced in rodents by exposure to chronic, unpredictable mild stress. This model demonstrates face validity in that exposed animals display signs similar to those of depressed humans, construct validity in that biochemical and cellular changes following CMS resemble similar changes in depressed patients, and predictive validity because the neurobiological and behavioral post CMS changes are effectively reversed by antidepressant drugs. The chronic mild stress procedure has been extensively implemented on both rats and mice.

Learned helplessness
This is a classic model of stress induced depression. In this paradigm, animals are exposed to a series of uncontrollable and inescapable stressors after which the animal develops a state of “helplessness” and does not attempt to escape even when the opportunity to do so is presented. Effects are manifested in reduced weight, reduced libido, deficits in tests evaluating cognition, anxiety and depression. The learned helplessness model is applicable to both mice and rats.

Social isolation
Early (post weaning) isolation in rodents is associated with inceased anxiety and depressive like phenotypes, locomotor dysregulation in novel environments and increased stress vulnerability During the implementation of this model, immediately after weaning, animals will be housed individualy for 6 weeks in cages. Mice that will be group housed in cages of the same size will serve as controls. This procedure has been used extensively in both mice and rats.

Social defeat
This test aims to evaluate the behavioral and biological consequences of chronic social stress- exposure to a dominant adversary. An aggressive retired breeder male is introduced into cages of test animals (housed 3 per cage) for six consecutive days (2 hours session each). During each session, submissive behavior of resident animals (upright posture, fleeing and crouching) is observed. If the aggressive intruder does not initiate defeat of the resident animal within 10 minutes or is defeated by any of the resident animals, the intruder is removed. Different intruders are used each day.

GENETIC MODELS

Flinders sensitive line (FSL) rats
This strain serves as an animal model of depression because of several features that resemble human depressed states. Such features include increased REM sleep, increased passive/ inhibitory behavior following stress and increased immobility in the forced swim test that is readily reversed by antidepressant drugs (thereby providing predictive validity to this model).

Wistar Kyoto rats
This rat strain is used as an animal model of depression co-morbid with anxiety (as opposed to the Flinders pure depression model) and demonstrates hormonal, behavioral and physiological phenotypes resembling those found in depressed patients. The animals demonstrate increased immobility in the forced swim test, decreased activity in the open field and reduced responsiveness to antidepressants.

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Mouse
Rat
Autism Animal Models
Price on request

GENETIC

BTBR mice
BTBR T+tf/J is a genetically homogenous inbred mouse strain that display many autistic like behavioral phenotypes such as reduced reciprocal social interactions, impaired juvenile play, increased repetitive behaviors such as self-grooming and unusual ultrasonic vocalizations in pups and adults.

*Juvenile... Show more »

GENETIC

BTBR mice
BTBR T+tf/J is a genetically homogenous inbred mouse strain that display many autistic like behavioral phenotypes such as reduced reciprocal social interactions, impaired juvenile play, increased repetitive behaviors such as self-grooming and unusual ultrasonic vocalizations in pups and adults.

Juvenile BALB/c mice
Mice of this strain display, at the age of 30 days but not at more advanced ages, relatively low sociability, reduced reciprocal social interactions, reduced ultrasonic vocalizations in same sex social interaction, reduced place-conditioned social reward and reduced social learning during social distress.

ENVIRONMENTAL

Maternal Immune system activation
Maternal infection during pregnancy is an established epidemiological risk factor for the development of autism spectrum disorders (prenatal exposure to rubella, herpes influenza or CMV). The model encompasses exposure of pregnant dams to influenza virus or to non viral immunostimulants such as bacterial lipopoly saccharide and double strand RNA poly(I:C), producing prominent behavioral, histological and neurochemical abnormalities in the offspring correlating to findings in ASD patients. This paradigm was tested in both mice and rats and is applicable to both species.

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Mouse
Rat
Schizophrenia Animal Models
Price on request

PHARMACOLOGICAL

MK-801 induced hyperlocomotion

Administration of pharmacological agents acting on neurotransmitter system thought to be involved in the pathogenesis of schizophrenia is used in modeling this disorder. Antagonists of glutamatergic NMDA receptors such as MK-801 are used to create behavioral analogs of the... Show more »

PHARMACOLOGICAL

MK-801 induced hyperlocomotion

Administration of pharmacological agents acting on neurotransmitter system thought to be involved in the pathogenesis of schizophrenia is used in modeling this disorder. Antagonists of glutamatergic NMDA receptors such as MK-801 are used to create behavioral analogs of the positive symptoms of schizophrenia such as hyperlocomotion and pre-pulse inhibition deficits. This model is applicable to both mice and rats. This test evaluates pharmacologically induced glutamatergic hypoactivity. Animals are injected intraperitoneally with MK-801 0.15mg/kg or saline and 30 min after that are allowed to explore an open field arena for 6 minutes. Total distance moved, velocity and time spent in arena periphery and center are tracked and recorded.

GENETIC

DISC1 mutant mice

DISC1 is a well known candidate gene for schizophrenia. Transgenic mice with truncated DISC1 transcription (and hence lower protein levels) are characterized by deficits in working memory, executive functioning and spontaneous hyperlocomotion.

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Anxiety Animal Models
Price on request

Serotonin transporter knockout mouse
These mice demonstrate a range of behavioral and neurophysiological abnormalities that resemble symptoms of human anxiety disorders. They are characterized by reduced aggression, increased anxiety like behavior, altered behavioral despair responses and reduced social interaction.

*5HT1A... Show more »

Serotonin transporter knockout mouse
These mice demonstrate a range of behavioral and neurophysiological abnormalities that resemble symptoms of human anxiety disorders. They are characterized by reduced aggression, increased anxiety like behavior, altered behavioral despair responses and reduced social interaction.

5HT1A receptor knockout mouse
This mouse model expresses a severe anxiety phenotype in the elevated plus maze and open field tests which is highly consistent with the generally observed anxiolytic effects of 5-HT1A receptors. 5HT1A receptor knockout mouse are also characterized by increased avoidance of novel objects and reduced exploratory activity.

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Mouse
Prion Disease Animal Models
Price on request

Transmissible model (RML PrP infected mice)
Mice are infected with mouse prions either i.c. or i.p and followed for disease signs that first appear at about 10 and 150 days, respectably. Mice usually are sacrificed 10-165 days after the appearance of clinical signs. Biochemical and pathological examination reveal accumulation... Show more »

Transmissible model (RML PrP infected mice)
Mice are infected with mouse prions either i.c. or i.p and followed for disease signs that first appear at about 10 and 150 days, respectably. Mice usually are sacrificed 10-165 days after the appearance of clinical signs. Biochemical and pathological examination reveal accumulation of PrPSc in brains and spleens, gliosis and neuronal death.

Prion (TgMHu2ME199K) tg-mice
We use our TgMHu2ME199K mice, a model for genetic (E200K) Creutzfeldt-Jakob disease in Libyan Jews. Neurological dysfunction starts at 5 months. Neuronal abnormalities include vacuolation, gliosis, neuronal death, accumulation of the aberrant PrP protein (denominated PrPSc) as well as oxidation of lipids and reduced neuroenesis.

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Mouse
Custom Animal Models
Price on request

Combined genetic-environmental models
Available upon request

Combined genetic-environmental models
Available upon request

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Mouse
Animal Behavior Studies
Price on request

Hadassah BrainLabs offers a comprehensive series of animal behavioral tests to study novel compounds, discover new indications for existing compounds and phenotypically characterize genetically modified mice. Individual tests may be selected from within each panel of behavioral tests and combined with tests from other panels in... Show more »

Hadassah BrainLabs offers a comprehensive series of animal behavioral tests to study novel compounds, discover new indications for existing compounds and phenotypically characterize genetically modified mice. Individual tests may be selected from within each panel of behavioral tests and combined with tests from other panels in accordance with the preference of the investigator.

Cognition, learning and memory test panel
Social behavior test panel
Depression test panel
Anxiety test panel
Psychosis test panel
Neurological test panel (Motor scores)
Neurological test panel (EAE, ALS, prion, neurological severity, myasthenia gravis scores)

These behavioral test panels may be paired with animal models of neurological and psychiatric disorders offered by Hadassah BrainLabs:

Alzheimer animal model
Tauopathy animal model
Multiple sclerosis animal model
Myasthenia gravis animal model
Amyotrophic lateral sclerosis (ALS) animal model
Depression animal model
Autism animal model
Schizophrenia animal model
Anxiety disorders animal model
Prion disease animal model

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Mouse
Rat
Cognition and Behavior Animal Models
Price on request

Novel object recognition
This test, by evaluating the differences in the exploration time of novel and familiar objects, provides a measure for cognition, especially for recognition and episodic memory. The animal is put inside a transparent plexiglass box 50cm all dimensions, with two similar “familiar” objects for a... Show more »

Novel object recognition
This test, by evaluating the differences in the exploration time of novel and familiar objects, provides a measure for cognition, especially for recognition and episodic memory. The animal is put inside a transparent plexiglass box 50cm all dimensions, with two similar “familiar” objects for a habituation stage of 10 minutes. After an hour the animal is re-entered to the arena for a 5 minute test session, now with one “familiar” and one different “novel” object. Duration of time spent near each object, and the frequency and number of approaches are tracked. [This test is applicable to rats and mice]

Radial arm water maze
Evaluates working memory, spatial learning and cognitive ability. The apparatus consists of 6 arms, 30 cm in length converging on a central 40X40 cm pool, filled with water to cover a plexiglass platform. On day 1 of the protocol the animal is trained to locate the platform (15 trials of up to 1 minute, alternating between hidden and visible platformS). On day 2 (test) all 15 trials are with the platform hidden. Entry into the wrong arm or non-entry into the correct arm after 15 seconds are designated as errors. Number of errors for each animal is tracked and scored. [This test is applicable to rats and mice]

Spontaneous alternation in T-maze
This test evaluates cognition, especially spatial and working memory. The animal is inserted into the start arm of a T shaped maze and allowed to move freely. On trial 1, the animal is allowed to enter either arm, and thereafter confined there for 5 seconds. Then it is returned to the start arm and the subsequent arm choice is recorded (trial 2), followed by a further 13 choice trials (in total 15 trials). Percentage of animals per group re-entering the same arm is an indicator of a repetitive behavior. [This test is applicable to rats and mice]

Morris water maze
This test evaluates cognitive ability, especially short term (cortical) and longer term (hippocampal) working memory and spatial learning. The test apparatus is a round metal pool, filled with an opaque water to a height that covers a plexiglass platform 16X16 cm located 20 cm from the pool edge. In acquisition trials (4 consecutive for each animal, repeated for 5 days), the time required for the animal to find the platform is tracked and recorded. Reversal trials consist of time taken to find the platform relocated to the opposite side of pool. Probe trials consist of trials in clear water, without the platform, in which the time spent by the animal in each of four quadrants of the pool is tracked and recorded. [This test is applicable to rats (pool diameter 140cm, height 60 cm) and mice (pool diameter 120 cm, height 40 cm)]

Fear conditioning
This test aims to evaluate fear memory- contextual (hippocampus mediated) and cued (amygdale mediated). The test device consists of two compartments and has a grid floor capable of generating low electrical current for a given time period. The animal is put inside the device and receives a 2 second, 0.5 mA shock coupled to a tone for two repeats. After 48 hours, the freezing time of the animal after exposure to either the acoustic tone (cued fear conditioning) or the visual context (context fear conditioning) is measured out of a total 5 minute exposure. [This test is applicable to rats and mice]

Attentional set shifting
This test aims to evaluate animal ability of the animal to learn rules associated with finding food, and its cognitive flexibility in re-learning as these rules are changed. Animals are first acclimated to a plexiglass chamber. Thereafter, two bowls are presented with different odors/mediums, one of them baited. Trials consist of the animal able to discern which of the cups contains food using simple discrimination (odor and medium), compound discrimination rules. Thereafter, the animals are required to adjust to changes in the location of the food (from one bowl to the other) according to intra-dimensional (same dimension- odor or medium change), and extra-dimensional (reward for medium instead of odor and vice versa) rules. For each session, number of correct choices and errors are recorded.

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Mouse
Rat
Animal Social Behavior Test Panel
Price on request

Three chambers sociability and social novelty test
This test aims to to evaluate animal levels of sociability (preference of an unfamiliar mouse over an object) and preference for social novelty (preference of a novel stranger over a familiar one).The device used in this test consists of three chambers (left, right and... Show more »

Three chambers sociability and social novelty test
This test aims to to evaluate animal levels of sociability (preference of an unfamiliar mouse over an object) and preference for social novelty (preference of a novel stranger over a familiar one).The device used in this test consists of three chambers (left, right and central); the central chamber is connected to the others with doors. In the habituation phase, the animal is allowed to explore the device freely for 10 minutes. In the second, sociability phase, a stranger mouse is placed in one of the lateral chambers (inside a specially devised cup with bars). In the social novelty phase another stranger is similarly introduced into the other lateral chamber. During phases 2 and 3, time spent in each chamber, number of approaches to each stranger mouse and their frequency are tracked and recorded. [This test is applicable to mice only]

Social interaction in pairs
The aim of this test is to investigate levels of sociability displayed in mutual social exploration of two hitherto unfamiliar animals. The test is performed in a neutral cage. Before the test, each individual animal goes through a 10 min session in the neutral cage alone (habituation to test conditions). After habituation, the two unfamiliar animals are placed together in the neutral cage for 10 min. The time and frequency spent in different kinds of social interactions is measured. [This test is applicable to rats and mice]

Resident intruder test
In this test for aggressive social interaction, same sex mice are first individually housed (for 7 days before testing) as cage resident mice. As the intruder mouse a group caged same sex and age mouse is selected. The intruder is introduced into the cage of the test resident mouse. The observation starts when the resident first sniffs the intruder. The observation stops when the first attack (by either mouse) occurs, or when no attack has occurred by 5 minutes observation. [This test is applicable to mice only]

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Mouse
Animal Depression Tests
Price on request

Forced swim test
This test is used both as a screening test for the effect of antidepressants to increase mobility, and as a learned helplessness measure of depressive like features. The animal is placed in a water-filled, transparent, round plexiglass tank. For the duration of the 6 minute test period, the time spent by the... Show more »

Forced swim test
This test is used both as a screening test for the effect of antidepressants to increase mobility, and as a learned helplessness measure of depressive like features. The animal is placed in a water-filled, transparent, round plexiglass tank. For the duration of the 6 minute test period, the time spent by the animal in activity (active swimming) versus immobility (passive floating) is tracked and recorded, as well as frequency and duration of activity/ inactivity bouts. [This test is applicable to rats and mice]

Sucrose preference test
This test models the depressive symptom of anhedonia (lack of pleasure). Non-preference for sweetened water is conceptualized as anhedonia. In the habituation phase, animals are group housed and receive fluids from two bottles, one containing water and the other 2% sucrose solution in water. In the 2 day test period, animals have a choice between two weighted bottles as above. After the test, the two bottles for each animal are weighed again and saccharin preference is calculated as percentage of sucrose consumed out total fluids consumed. [This test is applicable to rats and mice]

Novelty suppressed feeding test
This test models the depressive symptom of anhedonia (lack of pleasure). Long latency of a food deprived animal to eat a pellet of food is a measure of an anhedonic depression like phenotype. Animals are first food (but not water) deprived for 24 hours. Thereafter each is introduced into an open field arena in which a food pellet is situated in the center. The time elapsed from the animal’s introduction until eating commences (latency to eat) is tracked and recorded. Concomitantly, the distance moved by the animal is tracked. After testing home cage food consumption is monitored for 10 minutes. [This test is applicable to rats and mice]

Tail suspension test
This test is used both as a screening test for the activity of antidepressant to increase mobiity and as a learned helplessness measure of depressive like features. The animal is hung upside down by its tail tip (using adhesive tape) 50 cm from nearest surface ,and during a 6 minute test duration the time spent in active escape attempts is tracked and recorded (similarly to the forced swim test) versus time spent in inactivity. Also, frequency and duration of activity bouts are recorded. [This test is applicable for mice only]

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Mouse
Rat
Animal Anxiety Tests
Price on request

Elevated plus maze
This test is an approach avoidance test evaluating an anxiety like phenotype by monitoring animal levels of risk avoidance (of maze open arms). The test apparatus consists of four arms- two closed (enclosed by high side walls) and perpendicular to them two open arms, all four converging on a central platform.... Show more »

Elevated plus maze
This test is an approach avoidance test evaluating an anxiety like phenotype by monitoring animal levels of risk avoidance (of maze open arms). The test apparatus consists of four arms- two closed (enclosed by high side walls) and perpendicular to them two open arms, all four converging on a central platform. This construction is elevated 75 cm from the ground. The animal is placed on the central platform, and over 6 minutes the time spent in the open and closed arms, arms entries and transitions from each arm kind to the other are tracked and recorded. The apparatus for rats consist of four arms measuring 50 × 10 cm, The closed arms are enclosed by walls 40-cm high, except for the entrance. [This test is applicable to rats and mice]

Stress induced hyperthermia
This test evaluates an animal’s level of stress/arousal by measuring the extent of stress-induced temperature elevation. A stainless steel rectal probe for rodent temperature measurement (Harvard Apparatus, Holliston, MA, USA) connected to a Thermalert TH-5 thermometer is dipped in mineral oil and then inserted into the rectum, and temperature is measured as baseline value. 10 minutes later, hyperthermia resulting from the stress induced by the first measurement is measured. [This test is applicable to mice only]

Open field
This test is an approach avoidance test evaluating anxiety an like phenotype by monitoring the animal levels of risk avoidance (of the open arena center). The animal is introduced into an open field arena. Arena center and periphery are defined using the EthoVision arena setting tools. Over 6 minutes, distance moved by the animal, its velocity, time spent in the periphery or center of the arena and frequency of entries into each zone are tracked and recorded. [This test is applicable to rats and mice]

Startle response
This test evaluates an animal’s level of stress/ arousal by measuring the extent of auditory tone induced flinching. This test is conducted in the same device used for prepulse inhibition studies. Startle trials consist of a single noise burst (110 dB, 40ms), thereafter the amplitude of animal flinch is recorded. [This test is applicable to rats and mice]

Light dark box
This test is an approach avoidance test evaluating an anxiety like phenotype by monitoring the animal’s levels of risk avoidance (the open- lightened compartment of the light dark box). The device used in this test consists of two distinct compartments, interconnected by a small opening. One compartment is open-lighted, and the other is closed-dark. Over 10 minutes, time spent in each compartment and frequency and number of transitions between the two compartments are tracked and recorded. [This test is applicable to mice only]

Peripheral stress-related biomarkers (serum corticosterone, ACTH)
This test evaluates the animal’s level of stress/ arousal by measuring the extent of stress induced elevation serum corticpsterone. 30 minutes after exposure to stress, animals are anesthetized using veterinary pental, the heart is surgically exposed, blood is drawn (by the applicable technique for rats and mice), centrifuged and serum separated. Serum corticosterone and ACTH content are assayed. [This test is applicable to rats and mice]

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Mouse
Rat
Animal Model Psychosis Test Panel
Price on request

MK801-induced hyperlocomotion
This test evaluates pharmacologically induced glutamatergic hypoactivity. Animals are injected intraperitoneally with MK-801 0.15mg/kg or saline and 30 min after that are allowed to explore an open field arena for 6 minutes. Total distance moved, velocity and time spent in arena periphery and... Show more »

MK801-induced hyperlocomotion
This test evaluates pharmacologically induced glutamatergic hypoactivity. Animals are injected intraperitoneally with MK-801 0.15mg/kg or saline and 30 min after that are allowed to explore an open field arena for 6 minutes. Total distance moved, velocity and time spent in arena periphery and center are tracked and recorded. [This test is applicable to rats and mice]

MK-801-induced pre-pulse inhibition
This test evaluates the psychotic like effects of a pharmacological challenge (NMDA receptor antagonist) by studying the of the effect on pre pulse inhibition disruption. Animals are injected with MK-801 0.15mg/kg or saline, and 30 minutes later the test begins. Prepulse-pulse trials (PPI) consist of a prepulse of an intensity 2, 4, 8, or 16dB above background noise, followed 100 ms later by a startling pulse (110dB, 40ms), Sessions are designed to include acclimation, pre pulses and pulse trials. [This test is applicable to rats and mice]

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Animal Model Neurological Test Panel
Price on request

EAE score
This score evaluates the severity of symptoms in an animal model of induced experimental autoimmune encephalomyelitis. Animals are scored daily for neurological symptoms according to the EAE clinical severity scale: 0 = asymptomatic; 1 = partial loss of tail tonicity; 2 = tail paralysis; 3 = hind limb weakness; 4 =... Show more »

EAE score
This score evaluates the severity of symptoms in an animal model of induced experimental autoimmune encephalomyelitis. Animals are scored daily for neurological symptoms according to the EAE clinical severity scale: 0 = asymptomatic; 1 = partial loss of tail tonicity; 2 = tail paralysis; 3 = hind limb weakness; 4 = hind limb paralysis; 5 = 4-limb paralysis; 6 = death. [This test is applicable to rats and mice].

ALS score
This test evaluates the severity of amyotrophic lateral sclerosis in a genetic animal ALS model. Onset of disease-related weakness is defined as a sustained decrease of more than 30% of baseline maximum running distance. “Survival” is determined by an artificial endpoint: mice unable to right themselves 30 seconds after being placed on their sides are scored as “dead” and sacrificed. [This test is applicable to rats and mice].

Prion score
This test evaluate the progression and development of symptom severity in mice infected with prion disease pathogens. The score is based on the following landmarks: (1)- hind limbs weakness. (2)- hind limb partial paralysis. (3)- full paralysis in one limb. (4) Full hind limb paralysis. (5)- Death. [This test is applicable mice only]

Neurological severity score (NSS)
This score evaluate the effect of disease model on overall neurological function, including motor function, alertness and behavior. The score consists of 10 individual clinical parameters including motor function, alertness, and physiological behavior. 1 point is given for failure on the task and no points for succeeding . A maximal NSS of 10 points indicates severe neurological dysfunction, with failure on all tasks. [This test is applicable to rats and mice]

Myasthenia gravis score
This scale enables us to compare the clinical severity of myasthenia gravis symptoms in experimental rodents. There are four categories of severity of motor impairments and changes in body weight. The Mysthenia Gravis Score has the following categories ” (0) no weakness or fatigue, (1) mildly decreased activity, weak grip with fatigue, weight loss >3% body weight in one week; (2) moderate weakness accompanied by weak grip, 5-10%, weight loss (3) moderate-severe weakness, hunched back posture at rest, head down and forelimb digit flexed, tremulous ambulation,10% weight loss; and (4) severe general weakness, weak grip, weight loss >10%.”

Open field
This test evaluates animal motor activity as well as anxiety levels. Animals are placed in the corner of an arena. For the determined period of time, total distance traversed, velocity and sojourn in arena center and periphery are tracked and calculated. [This test is applicable to rats and mice].

Catalepsy test
This test evaluates the presence of features such as rigidity and difficulty in movement. Animal’s forepaws are placed over a horizontal bar at a height of 5 cm. Time elapsing from placement of the paws until both are removed by the animal is recorded, with a cutoff of 2minues. Trials are repeated (at 20 minute intervals) 3 times for each animal and averages are calculated. [This test is applicable to rats and mice].

Grip test
This test evaluateS the effect of neuromuscular disease on muscle strength and function. Total peak force (in gram force/gram body weight) is determined using an electronic Grip Strength Meter. Muscle strength measurements of both fore- and hind limbs are performed, with five measurements done for each animal. The three highest measurements are averaged to give the strength score. Animals are allowed to rest for 10 minutes between fore- and hind limb measurements.[This test is applicable to rats and mice].

Rotarod
This test evaluates motor-associated functions such as freedom of movement, flexibility, endurance and balance. In a day long test animals are placed on the rotarod and the rotation of the device is accelerated from 2.5 rpm to maximal 40 rpm during 4 minutes. Latency to fall from the device is measured unless the 4 minutes cutoff time has elapsed. Three trials are conducted (20 minute intervals) and averages are calculated. [This test is applicable to rats and mice].

Cylinder test
This test aims to determine the presence and extent of movement lateralization, indicative on unilateral neurological damage. The animal is placed in a clear plexiglass cylinder. Each contact of the forepaws (either left or right) with the cylinder (vertical exploration movements) is scored, and simultaneous contacts with both forepaws are scored independently. The laterality index is calculated as: [contacts(left)-contacts(right)]/[contacts(left)+contacts(right)+contacts(both forepaws)]. [This test is applicable to rats and mice]

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Animal Immunology Assays
Price on request

Lymphocyte proliferation assay using thymidine incorporation
Lymphocyte proliferation assay measures the ability of lymphocytes in short-term tissue culture to undergo proliferation when stimulated in vitro by a foreign antigen. The 3H-thymidine incorporation assay uses a technique wherein a radioactive nucleoside, 3... Show more »

Lymphocyte proliferation assay using thymidine incorporation
Lymphocyte proliferation assay measures the ability of lymphocytes in short-term tissue culture to undergo proliferation when stimulated in vitro by a foreign antigen. The 3H-thymidine incorporation assay uses a technique wherein a radioactive nucleoside, 3 H-thymidine, is incorporated into new strands of chromosomal DNA during mitotic cell division. A scintillation beta-counter is used to measure the radioactivity in DNA recovered from the cells in order to determine the extent of cell division that has occurred in response to an antigen/mitogen. The amount of radioactivity incorporated into DNA in each well is measured in a scintillation counter and is proportional to the number of proliferating cells, which in turn is a function of the number of lymphocytes that were stimulated by a given antigen to undergo a proliferative response. The readout is counts per minute (cpm) per well.

Flow cytometry analysis
Flow cytometry is a technique for characterizing cells and quantifying aspects about their components (e.g. size, and surface markers). Samples of cells, stained with floro-labelled antibodies to cell-surface or intracellularr molecules or proteins, are transferred into flow cytometer machine (BECKMAN COLTURE FC500) and data of the cells upon the specific markers are collected and analyzed.

Cytokine measurements
Different types of cytokines, chemokines and other proteins are measured using in-house ELISA detection kits or ready-to-use ELISA kits. Specific types of human chemokines and cytokines can be measured using FACS by the use of Cytometric Bead Array (CBA) read-to-use kits.

Antibody titers
The titer of different types of antibodies (Abs) in body fluids (like serum) can be determined by ELISA.

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Small Animal Functional Brain Imaging
Price on request

Micro-PET
Until recently data at the molecular level were obtained ex-vivo by sacrifice of large number of animals at several time points to obtain accurate kinetics. PET methodology allows for acquiring all the above dynamic data with high time resolution at the molecular level in-vivo without sacrifice of large number of... Show more »

Micro-PET
Until recently data at the molecular level were obtained ex-vivo by sacrifice of large number of animals at several time points to obtain accurate kinetics. PET methodology allows for acquiring all the above dynamic data with high time resolution at the molecular level in-vivo without sacrifice of large number of animals. Micro-PET equipment allows for optimal application of the PET imaging technology to animal research in the field of molecular biology and in particular in the field of neurology. The PET system is SPF approved.

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Mouse
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Single Nucleotide Polymorphism (SNP) Genotyping
Price on request

Genotyping analysis using: real time PCR for identifying single nucleotide polymorphism (SNP), capillary electorpherograms of PCR for microsatellite loci, as well as allele specific PCR and restriction enzyme analysis.

Genotyping analysis using: real time PCR for identifying single nucleotide polymorphism (SNP), capillary electorpherograms of PCR for microsatellite loci, as well as allele specific PCR and restriction enzyme analysis.

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Forced Swim Test
Price on request

The Forced Swim test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Depression panel

This test is used both as a screening test for the effect of antidepressants to increase mobility, and as a learned helplessness measure of depressive like features. The animal is placed in a water-filled, transparent,... Show more »

The Forced Swim test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Depression panel

This test is used both as a screening test for the effect of antidepressants to increase mobility, and as a learned helplessness measure of depressive like features. The animal is placed in a water-filled, transparent, round plexiglass tank. For the duration of the 6 minute test period, the time spent by the animal in activity (active swimming) versus immobility (passive floating) is tracked and recorded, as well as frequency and duration of activity/ inactivity bouts. [This test is applicable to rats and mice]

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Mouse
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Tail Suspension Test
Price on request

The Tail Suspension test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Depression panel

This test is used both as a screening test for the activity of antidepressant to increase mobiity and as a learned helplessness measure of depressive like features. The animal is hung upside down by its tail tip (using... Show more »

The Tail Suspension test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Depression panel

This test is used both as a screening test for the activity of antidepressant to increase mobiity and as a learned helplessness measure of depressive like features. The animal is hung upside down by its tail tip (using adhesive tape) 50 cm from nearest surface ,and during a 6 minute test duration the time spent in active escape attempts is tracked and recorded (similarly to the forced swim test) versus time spent in inactivity. Also, frequency and duration of activity bouts are recorded. [This test is applicable for mice only]

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Mouse
Novel Object Recognition Test
Price on request

The Novel Object Recognition test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Cognition, Learning and Memory panel

By evaluating differences in the exploration time of novel and familiar objects, this test provides a measure for cognition, especially for recognition and episodic memory. The animal is... Show more »

The Novel Object Recognition test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Cognition, Learning and Memory panel

By evaluating differences in the exploration time of novel and familiar objects, this test provides a measure for cognition, especially for recognition and episodic memory. The animal is put inside a transparent plexiglass box 50cm all dimensions, with two similar “familiar” objects for a habituation stage of 10 minutes. After an hour the animal is re-entered to the arena for a 5 minute test session, now with one “familiar” and one different “novel” object. Duration of time spent near each object, and the frequency and number of approaches are tracked. [This test is applicable to rats and mice]

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Animal Social Domain Tests
Price on request

The Three Chambers Sociability and Social Novelty test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Social Behavior panel

This test aims to to evaluate animal levels of sociability (preference of an unfamiliar mouse over an object) and preference for social novelty (preference of a novel stranger over a... Show more »

The Three Chambers Sociability and Social Novelty test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Social Behavior panel

This test aims to to evaluate animal levels of sociability (preference of an unfamiliar mouse over an object) and preference for social novelty (preference of a novel stranger over a familiar one).The device used in this test consists of three chambers (left, right and central); the central chamber is connected to the others with doors. In the habituation phase, the animal is allowed to explore the device freely for 10 minutes. In the second, sociability phase, a stranger mouse is placed in one of the lateral chambers (inside a specially devised cup with bars). In the social novelty phase another stranger is similarly introduced into the other lateral chamber. During phases 2 and 3, time spent in each chamber, number of approaches to each stranger mouse and their frequency are tracked and recorded. [This test is applicable to mice only]

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Mouse
Animal Model Myasthenia Gravis Scoring
Price on request

The Myasthenia Gravis score is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Neurological panel (EAE, ALS, prion, neurological severity, myasthenia gravis scores)

This scale enables us to compare the clinical severity of myasthenia gravis symptoms in experimental rodents. There are four categories of... Show more »

The Myasthenia Gravis score is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Neurological panel (EAE, ALS, prion, neurological severity, myasthenia gravis scores)

This scale enables us to compare the clinical severity of myasthenia gravis symptoms in experimental rodents. There are four categories of severity of motor impairments and changes in body weight. The Mysthenia Gravis Score has the following categories ” (0) no weakness or fatigue, (1) mildly decreased activity, weak grip with fatigue, weight loss >3% body weight in one week; (2) moderate weakness accompanied by weak grip, 5-10%, weight loss (3) moderate-severe weakness, hunched back posture at rest, head down and forelimb digit flexed, tremulous ambulation,10% weight loss; and (4) severe general weakness, weak grip, weight loss >10%.

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Mouse
Rat
Animal Model Neurological Severity Scoring
Price on request

The Neurological Severity (NSS) score is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Neurological panel (EAE, ALS, prion, neurological severity, myasthenia gravis scores)

This score evaluate the effect of disease model on overall neurological function, including motor function, alertness and behavior. The... Show more »

The Neurological Severity (NSS) score is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Neurological panel (EAE, ALS, prion, neurological severity, myasthenia gravis scores)

This score evaluate the effect of disease model on overall neurological function, including motor function, alertness and behavior. The score consists of 10 individual clinical parameters including motor function, alertness, and physiological behavior. 1 point is given for failure on the task and no points for succeeding . A maximal NSS of 10 points indicates severe neurological dysfunction, with failure on all tasks. [This test is applicable to rats and mice]

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Mouse
Rat
Prion score
Price on request

The Prion score is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Neurological test panel (EAE, ALS, prion, neurological severity, myasthenia gravis scores)

This test evaluates the progression and development of symptom severity in mice infected with prion disease pathogens. The score is based on the... Show more »

The Prion score is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Neurological test panel (EAE, ALS, prion, neurological severity, myasthenia gravis scores)

This test evaluates the progression and development of symptom severity in mice infected with prion disease pathogens. The score is based on the following landmarks: (1)- hind limbs weakness. (2)- hind limb partial paralysis. (3)- full paralysis in one limb. (4) Full hind limb paralysis. (5)- Death. [This test is applicable mice only]

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Mouse
Animal Model ALS Scoring
Price on request

The ALS score is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Neurological panel (EAE, ALS, prion, neurological severity, myasthenia gravis scores)

This test evaluates the severity of amyotrophic lateral sclerosis in a genetic animal ALS model. Onset of disease-related weakness is defined as a sustained... Show more »

The ALS score is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Neurological panel (EAE, ALS, prion, neurological severity, myasthenia gravis scores)

This test evaluates the severity of amyotrophic lateral sclerosis in a genetic animal ALS model. Onset of disease-related weakness is defined as a sustained decrease of more than 30% of baseline maximum running distance. “Survival” is determined by an artificial endpoint: mice unable to right themselves 30 seconds after being placed on their sides are scored as “dead” and sacrificed. [This test is applicable to rats and mice].

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Cylinder Test
Price on request

The Cylinder test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Neurological panel (motor scores)

This test aims to determine the presence and extent of movement lateralization, indicative on unilateral neurological damage. The animal is placed in a clear plexiglass cylinder. Each contact of the forepaws... Show more »

The Cylinder test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Neurological panel (motor scores)

This test aims to determine the presence and extent of movement lateralization, indicative on unilateral neurological damage. The animal is placed in a clear plexiglass cylinder. Each contact of the forepaws (either left or right) with the cylinder (vertical exploration movements) is scored, and simultaneous contacts with both forepaws are scored independently. The laterality index is calculated as: [contacts(left) – contacts (right)] / [contacts(left) + contacts(right) + contacts(both forepaws)]. [This test is applicable to rats and mice]

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Mouse
Rat
Rotarod Performance Test
Price on request

The Rotarod test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Neurological panel (motor scores)

This test evaluates motor-associated functions such as freedom of movement, flexibility, endurance and balance. In a day long test animals are placed on the rotarod and the rotation of the device is... Show more »

The Rotarod test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Neurological panel (motor scores)

This test evaluates motor-associated functions such as freedom of movement, flexibility, endurance and balance. In a day long test animals are placed on the rotarod and the rotation of the device is accelerated from 2.5 rpm to maximal 40 rpm during 4 minutes. Latency to fall from the device is measured unless the 4 minutes cutoff time has elapsed. Three trials are conducted (20 minute intervals) and averages are calculated. [This test is applicable to rats and mice].

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Mouse
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Animal Grip Strength Test
Price on request

The Grip test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Neurological panel (motor scores)

This test evaluates the effect of neuromuscular disease on muscle strength and function. Total peak force (in gram force/gram body weight) is determined using an electronic Grip Strength Meter. Muscle strength... Show more »

The Grip test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Neurological panel (motor scores)

This test evaluates the effect of neuromuscular disease on muscle strength and function. Total peak force (in gram force/gram body weight) is determined using an electronic Grip Strength Meter. Muscle strength measurements of both fore- and hind limbs are performed, with five measurements done for each animal. The three highest measurements are averaged to give the strength score. Animals are allowed to rest for 10 minutes between fore- and hind limb measurements.[This test is applicable to rats and mice].

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Mouse
Rat
Catalepsy Test
Price on request

The Catalepsy test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Neurological panel (motor scores)

This test evaluates the presence of features such as rigidity and difficulty in movement. Animal’s forepaws are placed over a horizontal bar at a height of 5 cm. Time elapsing from placement of the paws... Show more »

The Catalepsy test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Neurological panel (motor scores)

This test evaluates the presence of features such as rigidity and difficulty in movement. Animal’s forepaws are placed over a horizontal bar at a height of 5 cm. Time elapsing from placement of the paws until both are removed by the animal is recorded, with a cutoff of 2minues. Trials are repeated (at 20 minute intervals) 3 times for each animal and averages are calculated. [This test is applicable to rats and mice].

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Mouse
Rat
Resident Intruder Test
Price on request

The Resident Intruder test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Social Behavior panel

In this test for aggressive social interaction, same sex mice are first individually housed (for 7 days before testing) as cage resident mice. As the intruder mouse a group caged same sex and age mouse is... Show more »

The Resident Intruder test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Social Behavior panel

In this test for aggressive social interaction, same sex mice are first individually housed (for 7 days before testing) as cage resident mice. As the intruder mouse a group caged same sex and age mouse is selected. The intruder is introduced into the cage of the test resident mouse. The observation starts when the resident first sniffs the intruder. The observation stops when the first attack (by either mouse) occurs, or when no attack has occurred by 5 minutes observation. [This test is applicable to mice only]

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Mouse
Animal Social Interaction in Pairs Test
Price on request

The Social Interaction in Pairs test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Social Behavior panel

The aim of this test is to investigate levels of sociability displayed in mutual social exploration of two hitherto unfamiliar animals. The test is performed in a neutral cage. Before the test, each... Show more »

The Social Interaction in Pairs test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Social Behavior panel

The aim of this test is to investigate levels of sociability displayed in mutual social exploration of two hitherto unfamiliar animals. The test is performed in a neutral cage. Before the test, each individual animal goes through a 10 min session in the neutral cage alone (habituation to test conditions). After habituation, the two unfamiliar animals are placed together in the neutral cage for 10 min. The time and frequency spent in different kinds of social interactions is measured. [This test is applicable to rats and mice]

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Mouse
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Animal Chronic Stress Tests
Price on request

The Peripheral Stress-Related Biomarkers test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Anxiety panel

This test evaluates the animal’s level of stress/ arousal by measuring the extent of stress induced elevation serum corticosterone. 30 minutes after exposure to stress, animals are anesthetized using... Show more »

The Peripheral Stress-Related Biomarkers test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Anxiety panel

This test evaluates the animal’s level of stress/ arousal by measuring the extent of stress induced elevation serum corticosterone. 30 minutes after exposure to stress, animals are anesthetized using veterinary pental, the heart is surgically exposed, blood is drawn (by the applicable technique for rats and mice), centrifuged and serum separated. Serum corticosterone and ACTH content are assayed. [This test is applicable to rats and mice]

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Mouse
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Light/Dark Transition Assay
Price on request

The Light dark Box test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Anxiety panel

This test is an approach avoidance test evaluating an anxiety like phenotype by monitoring the animal’s levels of risk avoidance (the open- lightened compartment of the light dark box). The device used in this test... Show more »

The Light dark Box test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Anxiety panel

This test is an approach avoidance test evaluating an anxiety like phenotype by monitoring the animal’s levels of risk avoidance (the open- lightened compartment of the light dark box). The device used in this test consists of two distinct compartments, interconnected by a small opening. One compartment is open-lighted, and the other is closed-dark. Over 10 minutes, time spent in each compartment and frequency and number of transitions between the two compartments are tracked and recorded. [This test is applicable to mice only]

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Mouse
Open Field Test
Price on request

The Open Field test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Anxiety panel

This test is an approach avoidance test evaluating anxiety an like phenotype by monitoring the animal levels of risk avoidance (of the open arena center). The animal is introduced into an open field arena. Arena center and... Show more »

The Open Field test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Anxiety panel

This test is an approach avoidance test evaluating anxiety an like phenotype by monitoring the animal levels of risk avoidance (of the open arena center). The animal is introduced into an open field arena. Arena center and periphery are defined using the EthoVision arena setting tools. Over 6 minutes, distance moved by the animal, its velocity, time spent in the periphery or center of the arena and frequency of entries into each zone are tracked and recorded. [This test is applicable to rats and mice]

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Mouse
Rat
Startle Response Test
Price on request

The Startle Response test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Anxiety panel

This test evaluates an animal’s level of stress/ arousal by measuring the extent of auditory tone induced flinching. This test is conducted in the same device used for prepulse inhibition studies. Startle trials consist... Show more »

The Startle Response test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Anxiety panel

This test evaluates an animal’s level of stress/ arousal by measuring the extent of auditory tone induced flinching. This test is conducted in the same device used for prepulse inhibition studies. Startle trials consist of a single noise burst (110 dB, 40ms), thereafter the amplitude of animal flinch is recorded. [This test is applicable to rats and mice]

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Mouse
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Stress-Induced Hyperthermia Assay
Price on request

The Stress Induced Hyperthermia test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Anxiety panel

This test evaluates an animal’s level of stress/arousal by measuring the extent of stress-induced temperature elevation. A stainless steel rectal probe for rodent temperature measurement (Harvard Apparatus,... Show more »

The Stress Induced Hyperthermia test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Anxiety panel

This test evaluates an animal’s level of stress/arousal by measuring the extent of stress-induced temperature elevation. A stainless steel rectal probe for rodent temperature measurement (Harvard Apparatus, Holliston, MA, USA) connected to a Thermalert TH-5 thermometer is dipped in mineral oil and then inserted into the rectum, and temperature is measured as baseline value. 10 minutes later, hyperthermia resulting from the stress induced by the first measurement is measured. [This test is applicable to mice only]

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Mouse
Elevated Plus Maze Animal Anxiety Test
Price on request

The Elevated Plus Maze test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Anxiety panel

This test is an approach avoidance test evaluating an anxiety like phenotype by monitoring animal levels of risk avoidance (of maze open arms). The test apparatus consists of four arms- two closed (enclosed by high... Show more »

The Elevated Plus Maze test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Anxiety panel

This test is an approach avoidance test evaluating an anxiety like phenotype by monitoring animal levels of risk avoidance (of maze open arms). The test apparatus consists of four arms- two closed (enclosed by high side walls) and perpendicular to them two open arms, all four converging on a central platform. This construction is elevated 75 cm from the ground. The animal is placed on the central platform, and over 6 minutes the time spent in the open and closed arms, arms entries and transitions from each arm kind to the other are tracked and recorded. The apparatus for rats consist of four arms measuring 50 × 10 cm, The closed arms are enclosed by walls 40-cm high, except for the entrance. [This test is applicable to rats and mice]

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Mouse
Rat
Novel Environment Feeding Suppression
Price on request

The Novelty Suppressed Feeding test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Depression panel

This test models the depressive symptom of anhedonia (lack of pleasure). Long latency of a food deprived animal to eat a pellet of food is a measure of an anhedonic depression like phenotype. Animals are... Show more »

The Novelty Suppressed Feeding test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Depression panel

This test models the depressive symptom of anhedonia (lack of pleasure). Long latency of a food deprived animal to eat a pellet of food is a measure of an anhedonic depression like phenotype. Animals are first food (but not water) deprived for 24 hours. Thereafter each is introduced into an open field arena in which a food pellet is situated in the center. The time elapsed from the animal’s introduction until eating commences (latency to eat) is tracked and recorded. Concomitantly, the distance moved by the animal is tracked. After testing home cage food consumption is monitored for 10 minutes. [This test is applicable to rats and mice]

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Mouse
Rat
Sucrose Preference Test
Price on request

The Sucrose Preference test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Depression panel

This test models the depressive symptom of anhedonia (lack of pleasure). Non-preference for sweetened water is conceptualized as anhedonia. In the habituation phase, animals are group housed and receive fluids from... Show more »

The Sucrose Preference test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Depression panel

This test models the depressive symptom of anhedonia (lack of pleasure). Non-preference for sweetened water is conceptualized as anhedonia. In the habituation phase, animals are group housed and receive fluids from two bottles, one containing water and the other 2% sucrose solution in water. In the 2 day test period, animals have a choice between two weighted bottles as above. After the test, the two bottles for each animal are weighed again and saccharin preference is calculated as percentage of sucrose consumed out total fluids consumed. [This test is applicable to rats and mice]

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Mouse
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Attentional Set-Shifting Test
Price on request

The Attentional Set Shifting test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Cognition, Learning and Memory panel

This test aims to evaluate animal ability of the animal to learn rules associated with finding food, and its cognitive flexibility in re-learning as these rules are changed. Animals are... Show more »

The Attentional Set Shifting test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Cognition, Learning and Memory panel

This test aims to evaluate animal ability of the animal to learn rules associated with finding food, and its cognitive flexibility in re-learning as these rules are changed. Animals are first acclimated to a plexiglass chamber. Thereafter, two bowls are presented with different odors/mediums, one of them baited. Trials consist of the animal able to discern which of the cups contains food using simple discrimination (odor and medium), compound discrimination rules. Thereafter, the animals are required to adjust to changes in the location of the food (from one bowl to the other) according to intra-dimensional (same dimension- odor or medium change), and extra-dimensional (reward for medium instead of odor and vice versa) rules. For each session, number of correct choices and errors are recorded.

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Mouse
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Animal Fear Conditioning Studies
Price on request

The Fear Conditioning test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Cognition, Learning and Memory panel.

This test aims to evaluate fear memory- contextual (hippocampus mediated) and cued (amygdale mediated). The test device consists of two compartments and has a grid floor capable of generating low... Show more »

The Fear Conditioning test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Cognition, Learning and Memory panel.

This test aims to evaluate fear memory- contextual (hippocampus mediated) and cued (amygdale mediated). The test device consists of two compartments and has a grid floor capable of generating low electrical current for a given time period. The animal is put inside the device and receives a 2 second, 0.5 mA shock coupled to a tone for two repeats. After 48 hours, the freezing time of the animal after exposure to either the acoustic tone (cued fear conditioning) or the visual context (context fear conditioning) is measured out of a total 5 minute exposure. [This test is applicable to mice]

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Mouse
Morris Water Maze Assay
Price on request

The Morris Water Maze test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Cognition, Learning and Memory panel

This test evaluates cognitive ability, especially short term (cortical) and longer term (hippocampal) working memory and spatial learning. The test apparatus is a round metal pool, filled with an... Show more »

The Morris Water Maze test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Cognition, Learning and Memory panel

This test evaluates cognitive ability, especially short term (cortical) and longer term (hippocampal) working memory and spatial learning. The test apparatus is a round metal pool, filled with an opaque water to a height that covers a plexiglass platform 16X16 cm located 20 cm from the pool edge. In acquisition trials (4 consecutive for each animal, repeated for 5 days), the time required for the animal to find the platform is tracked and recorded. Reversal trials consist of time taken to find the platform relocated to the opposite side of pool. Probe trials consist of trials in clear water, without the platform, in which the time spent by the animal in each of four quadrants of the pool is tracked and recorded. [This test is applicable to rats (pool diameter 140cm, height 60 cm) and mice (pool diameter 120 cm, height 40 cm)]

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Mouse
Rat
T Maze Spontaneous Alternation Test
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The Spontaneous Alternation in T-maze test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Cognition, Learning and Memory panel

This test evaluates cognition, especially spatial and working memory. The animal is inserted into the start arm of a T shaped maze and allowed to move freely. On trial 1, the... Show more »

The Spontaneous Alternation in T-maze test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Cognition, Learning and Memory panel

This test evaluates cognition, especially spatial and working memory. The animal is inserted into the start arm of a T shaped maze and allowed to move freely. On trial 1, the animal is allowed to enter either arm, and thereafter confined there for 5 seconds. Then it is returned to the start arm and the subsequent arm choice is recorded (trial 2), followed by a further 13 choice trials (in total 15 trials). Percentage of animals per group re-entering the same arm is an indicator of a repetitive behavior. [This test is applicable to rats and mice]

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Mouse
Rat
Radial Arm Water Maze Test
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The Radial Arm Water Maze test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Cognition, Learning and Memory panel

This test evaluates working memory, spatial learning and cognitive ability. The apparatus consists of 6 arms, 30 cm in length converging on a central 40X40 cm pool, filled with water to cover... Show more »

The Radial Arm Water Maze test is part of the the Hadassah BrainLabs, Animal Behavior Analysis: Cognition, Learning and Memory panel

This test evaluates working memory, spatial learning and cognitive ability. The apparatus consists of 6 arms, 30 cm in length converging on a central 40X40 cm pool, filled with water to cover a plexiglass platform. On day 1 of the protocol the animal is trained to locate the platform (15 trials of up to 1 minute, alternating between hidden and visible platformS). On day 2 (test) all 15 trials are with the platform hidden. Entry into the wrong arm or non-entry into the correct arm after 15 seconds are designated as errors. Number of errors for each animal is tracked and scored. [This test is applicable to rats and mice]

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Mouse
Rat
Cell Proliferation Assays
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Lymphocyte Proliferation Assay is part of the Hadassah BrainLabs Rodent Immunological Markers Panel
Lymphocyte proliferation assay measures the ability of lymphocytes in short-term tissue culture to undergo proliferation when stimulated in vitro by a foreign antigen. The 3H-thymidine incorporation assay uses a technique... Show more »

Lymphocyte Proliferation Assay is part of the Hadassah BrainLabs Rodent Immunological Markers Panel
Lymphocyte proliferation assay measures the ability of lymphocytes in short-term tissue culture to undergo proliferation when stimulated in vitro by a foreign antigen. The 3H-thymidine incorporation assay uses a technique wherein a radioactive nucleoside, 3 H-thymidine, is incorporated into new strands of chromosomal DNA during mitotic cell division. A scintillation beta-counter is used to measure the radioactivity in DNA recovered from the cells in order to determine the extent of cell division that has occurred in response to an antigen/mitogen. The amount of radioactivity incorporated into DNA in each well is measured in a scintillation counter and is proportional to the number of proliferating cells, which in turn is a function of the number of lymphocytes that were stimulated by a given antigen to undergo a proliferative response. The readout is counts per minute (cpm) per well.

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Flow Cytometry
Price on request

Flow Cytometry is part of the Hadassah BrainLabs Rodent Immunological Markers Panel
Flow cytometry is a technique for characterizing cells and quantifying aspects about their components (e.g. size, and surface markers). Samples of cells, stained with floro-labelled antibodies to cell-surface or intracellularr molecules or... Show more »

Flow Cytometry is part of the Hadassah BrainLabs Rodent Immunological Markers Panel
Flow cytometry is a technique for characterizing cells and quantifying aspects about their components (e.g. size, and surface markers). Samples of cells, stained with floro-labelled antibodies to cell-surface or intracellularr molecules or proteins, are transferred into flow cytometer machine (BECKMAN COLTURE FC500) and data of the cells upon the specific markers are collected and analyzed.

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Cytokine Analysis
Price on request

Cytokine Quantification is part of the Hadassah BrainLabs Rodent Immunological Markers Panel
Different types of cytokines, chemokines and other proteins are measured using in-house ELISA detection kits or ready-to-use ELISA kits. Specific types of human chemokines and cytokines can be measured using FACS by the use of Cytometric Bead Array (CBA) read-to-use kits.

Cytokine Quantification is part of the Hadassah BrainLabs Rodent Immunological Markers Panel
Different types of cytokines, chemokines and other proteins are measured using in-house ELISA detection kits or ready-to-use ELISA kits. Specific types of human chemokines and cytokines can be measured using FACS by the use of Cytometric Bead Array (CBA) read-to-use kits.

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