Focused on medical education, research and community health outreach, Gundersen Medical Foundation is dedicated to advancing quality healthcare.
We are Recombineering Experts
The ability to clone herpesvirus genomes into a bacterial artificial chromosome (BAC) has improved the efficiency and fidelity of generating targeted mutations in these genomes. Over the past several years, new recombineering technologies have helped to streamline and improve our mutagenesis strategies. These new strategies were pioneered by Dr. Darby Oldenburg, PhD at Gundersen Medical Foundation. She has more than 10 years’ experience creating and characterizing various herpesvirus mutants for use in tissue culture and in vivo studies. Our methods provide robust, rapid and reliable mutations.
We use a combination of methods to create site-specific mutations (e.g. base-change, insertions, deletions, tagging and others) for DNA contained in BACs. We can create new BACs to study novel herpesvirus isolates as well as quickly and efficiently alter viral genomes in exiting BACs. As a result of this work, Dr. Oldenburg maintains a growing collection of herpesvirus BACs and molecular tools to expedite future mutagenesis projects.
The turnaround time (TAT) for a basic mutation such as a small insertion/deletion (less than 1kb) or DNA base substitution is about three weeks. This includes sequence analysis. The mutated virus is transferred to a stable E. coli strain for long-term storage.
We are always interested in new collaborative projects with researchers in the herpesvirus community who would like to have BACs and/or mutants created and characterized using our improved techniques.
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