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Girihlet Inc

New York, New York, US

Girihlet's patent pending technologies enables inexpensive , accurate and quick turn around time for sequencing MtDNA, T-Cell Receptors, and RNA specifically, (small RNA, tRNA, mRNA, non-ribosomal RNA, and even circular RNAs), plus data analysis. Turn around time <2 weeks.

Girihlet aims to make esoteric corners of the genome and transcriptome accessible to researchers and clinicians. We also offer expertise to help take your projects to completion, by providing innovative and creative solutions in the wet-lab and in the use of novel analytical approaches.

For complete publication record, please take a look here,

  1. Anitha Jayaprakash:. http://www.ncbi.nlm.nih.gov/sites/myncbi/1FuZCAWzn9bQg/bibliography/47774320/public/?sort=date&amp;direction=ascending

  2. Ravi Sachidanandam : http://www.ncbi.nlm.nih.gov/sites/myncbi/1zg6-w1AZ-OQO/bibliography/44998340/public/?sort=date&amp;direction=descending

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Sequencing Experimental Design
Starting at $100.00 per hour

Deep Sequencing Experimental Design

Our extensive experience with the deep sequencing platform allows us to design any experiment effectively and at a low price point. We keep in mind all the biases involved and down stream issues with analysis.

Deep Sequencing Experimental Design

Our extensive experience with the deep sequencing platform allows us to design any experiment effectively and at a low price point. We keep in mind all the biases involved and down stream issues with analysis.

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RNA Sequencing
Starting at $500.00 per sample

mRNA / non-ribosomal total RNA sequencing . (75 bp single end illumina sequencing, 40 million reads per sample). For analysis and normalized values , there is a 100 charge per sample.

mRNA / non-ribosomal total RNA sequencing . (75 bp single end illumina sequencing, 40 million reads per sample). For analysis and normalized values , there is a 100 charge per sample.

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Small RNA Sequencing
Starting at $300.00 per sample

We discovered the biases in commonly-used protocols and invented the remedy. Using our novel appraoches, we will provide 20 million reads (1*75bp) per sample. For reference, please read this paper, PMID: 21890899 (Identification and remediation of biases in the activity of RNA ligases in small-RNA deep sequencing. Jayaprakash et.al, NAR, 2011).

We discovered the biases in commonly-used protocols and invented the remedy. Using our novel appraoches, we will provide 20 million reads (1*75bp) per sample. For reference, please read this paper, PMID: 21890899 (Identification and remediation of biases in the activity of RNA ligases in small-RNA deep sequencing. Jayaprakash et.al, NAR, 2011).

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Bioinformatics
Starting at $125.00 per sample
  • small RNAs (piRNA, miRNA) Proprietary, widely-used tools for the analyses of these datasets, coupled with our small RNA sequencing protocol.
  • Variant calling A distinctive approach to variant calling from deep sequencing datasets
  • mRNA-splicing analyses Splice-site and novel variant analyses founded on extensive... Show more »
  • small RNAs (piRNA, miRNA) Proprietary, widely-used tools for the analyses of these datasets, coupled with our small RNA sequencing protocol.
  • Variant calling A distinctive approach to variant calling from deep sequencing datasets
  • mRNA-splicing analyses Splice-site and novel variant analyses founded on extensive mRNA-splicing experience [ Splice-site strengths , Comparative analysis and comprehensive database ]
  • De novo Assembly Based on experience with assemblies of transcriptomes of non-model organisms such as Bombyx mori and micro bats.
  • Expression profiling Extensive experience with mRNA-seq datasets from a variety of model organisms, including human, D. melanogaster, mouse and C. elegans
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Data Storage
Price on request

Using a mix of cloud and custom-built machines to store and make analyses accessible

Using a mix of cloud and custom-built machines to store and make analyses accessible

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Circular RNA Sequencing
Starting at $750.00 per sample

Through proprietary methods, we can efficiently sequence circular RNA from mouse / human samples. and will also provide linear RNA profiles simultaneously. The cost is inclusive of analysis, and an excel sheet with normalized values will be provided.

Through proprietary methods, we can efficiently sequence circular RNA from mouse / human samples. and will also provide linear RNA profiles simultaneously. The cost is inclusive of analysis, and an excel sheet with normalized values will be provided.

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TCR Repertoire Analysis
T-Cell Receptor Repertoire Analysis
Starting at $850.00 per sample

We can currently sequence T cell receptor sequences with high accuracy and efficiency. We use novel unbiased methods (not a mix of V primers), to amplify only the TCR sequences. Price is inclusive of analysis.

We can currently sequence T cell receptor sequences with high accuracy and efficiency. We use novel unbiased methods (not a mix of V primers), to amplify only the TCR sequences. Price is inclusive of analysis.

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Mitochondrial DNA Sequencing
Starting at $300.00 per sample

Mito-seq (mitochondrial DNA sequencing). We use novel approaches to isolate and sequence the mtDNA efficiently. We will provide 1*150 bp paired end sequencing, with a minimum 100X coverage.

Patented method of purifying and sequencing mtDNA for accurate determination of heteroplasmy

Mito-seq (mitochondrial DNA sequencing). We use novel approaches to isolate and sequence the mtDNA efficiently. We will provide 1*150 bp paired end sequencing, with a minimum 100X coverage.

Patented method of purifying and sequencing mtDNA for accurate determination of heteroplasmy

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Project and Process Management
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Experimental Design
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Biology
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Biochemistry & Molecular Biology
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Nucleic Acid Services
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DNA Services
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DNA Sequencing
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Next Generation Sequencing (NGS)
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Targeted Gene Sequencing
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RNA Services
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Computational Modeling
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Data Services
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Biostatistics & Bioinformatics
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