GenScript is the leading gene, peptide, protein and antibody research partner for fundamental life science research, translational biomedical research, and early stage pharmaceutical development. Since our establishment in 2002, GenScript has exponentially grown to become a global leading biotech company that provides life sciences services and products to scientists over 100 countries worldwide. During our tenure we have built the best-in-class capacity and capability for biological research services encompassing gene synthesis and molecular biology, peptide synthesis, custom antibodies, protein expression, antibody and protein engineering, and in vitro and in vivo pharmacology – all with the goal to Make Research Easy.
Key Facts about GenScript
We strive with competence and confidence to meet your demand for developing pre-clinical drug candidates time-efficiently and cost-effectively. With track performance record, GenScript is your ideal and reliable innovation partner in drug discovery.
Antibody humanization is the process to replace non-human frameworks of a non-human (usually) mAb with human ones. The key for a successful humanization is to maintain the affinity of the antibody while replacing the residues. GenScript’s unique antibody humanization service combines CDR-grafting, library-based proprietary ‘framework assembly’ method (US20120316085 (A1)) and FASEBA screening (FAst Screening for Expression level, Biophysical properties, and Affinities). The antibody can be humanized and at the same time engineered for better properties like expression level and thermostability.
Antibody therapy has been proven to be highly powerful for cancer treatment. Two important mechanisms used by antibody drugs to kill targeted tumor cells are Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC), and Complement Dependent Cytotoxicity (CDC). GenScript is pleased to present to you a recombinant effector cell-based assay. The readout is endpoint-driven (target cell lysis). The consistency of results is dramatically enhanced compared to PBMC-based ADCC assay. The turnaround time and the cost are both significantly reduced by replacing primary cells with recombinant cells as the source of effector cells. The key to GenScript's breakthough in ADCC is the development of a NK92 cell line expressing FcRγ3a (CD16a)*. If desired, a PBMC-based ADCC assay is also available.
The CDC assay uses normal human serum as the source of complement. By implementing strict QC standards, GenScript can provide the assurance of the efficacy and potency profiles of your therapeutic antibodies.
Stable cell lines are used in a variety of research applications such as recombinant protein and antibody production, assay development, gene editing, functional studies and more. At GenScript, we offer comprehensive stable cell line development capabilities that either begin with DNA provided by our clients or that are powered by our fast, high efficiency gene synthesis service that seamlessly integrates all steps of stable cell line generation, delivering high quality stable clones of your choice. Boost your productivity and improve your results by leveraging GenScript's top quality stable cell line services.
GenScript offers a full suite of high quality custom polyclonal antibody production services designed with your needs in mind. Since 2004, our team has produced over 12,000 high quality custom polyclonal antibodies from rabbit,mouse, rat, goat and chicken hosts. The PolyExpress™ Premium protein immunization packages carry an industry leading guarantee of positive Western blot and 1:64,000 ELISA titer. PolyExpress™ Basic, Silver, and Gold peptide immunization packages also carry the ELISA titer guarantee and can deliver your custom pAb in 45 days.
Key Features of PolyExpress™ Custom Polyclonal Antibody Production Services
Yeast fermentation is the most economical eukaryotic expression system for scale up production of both secreted and intracellular proteins that require post-translational modifications, including humanized antibodies. GenScript's YeastHIGH™ yeast fermentation and expression services utilize a proprietary yeast fermentation technology for high-yield and high quality recombinant protein production in yeast, by selecting stable and durable production yeast strains that resemble mammalian system the most. We are also equipped with scale-up yeast fermentation capacity up to 500 liters. In addition, as the largest gene synthesis supplier in the U.S., GenScript deploys free OptimumGene™ codon optimization specific to yeast strains such as Pichia pastoris and Saccharomyces cerevisiae to further enhance your recombinant protein yields.
Advantages of YeastHIGH™ yeast fermentation and expression services
GenScript offers an extremely quick and cost-efficient custom peptide array service to assist in your drug development research. Based on our advanced peptide array platform, we can also provide comprehensive downstream services such as eptiope mapping. If you need up to a milligram or more purified peptide (purity up to 95%) for epitope mapping, activity assays, or proteomic applications, view our rapid, high-throughput peptide library services.
For further efficiency and convenience, GenScript provides an instant online quotation system for peptide array services. In addition, GenScript provides six, free online peptide library/array design tools to help you generate different peptide arrays to assist your research design.
A peptide library is a systematic combination of a large number of different peptides widely used for protein-related studies. Peptide libraries are in ever increasing demand due to the expanding interest in peptide drug and vaccine development, immunotherapy, and proteomics. To accelerate your research, GenScript offers two peptide library services:
To make your research easier, GenScript also offers extensive QC for any peptide library order. As a part of the AccuPep QC service, you can request TFA removal, solubility testing, endotoxin analysis, or other services to ensure that your peptide library will meet your intended research application. In addition, our proprietary FlexPeptide™ technology ensures that hundreds of peptides can be synthesized at the lowest cost possible.
Our technical account managers, all with PhDs, will help you custom design the optimal protocol to meet your specific requirements. With GenScript's fully custom monoclonal antibody generation services, you are in control of every stage, deciding how to respond to results mid-project. You can evaluate and modify your approach, restart an entire phase, or terminate the project if you are not completely satisfied. Don't risk commitment of time and money to a rigid protocol when our fully custom monoclonal antibody generation service offers the ultimate flexibility. Partnering with GenScript's years of expertise can help you find success in your custom antibody development.
Hybridoma development is a powerful approach and the critical path to antibody drug lead generation. With 12 years’ experience in custom antibody production, GenScript has established a comprehensive antibody drug discovery platform under the leadership of the pioneers in the antibody drug discovery field. GenScript’s Premium Hybridoma Development Services utilize a portfolio of immunization approaches, including protein, whole cells and DNA immunization, high throughput binder screening and series of functional screening assays to generate up to 100 high quality hybridoma clones, assuring the success of your antibody drug development at the very beginning.
Key Features of Premium Hybridoma Development Services
Antibody production by hybridoma cell lines is inherently unstable. Failure to maintain cell lines properly will lead to loss in antibody productivity and eventually the cell line itself. Traditionally, hybridoma stabilization refers to the selection of stable clones for extended stable expression but the problem still remains. By integrating antibody engineering and bioprocess development technology, GenScript's Hybridoma Stabilization Service (Cat. No.: SC1511) can redesign the antibody for permanent preservation and improved productivity with advanced Hybridoma Revival System, and solve the inherent defect of antibody generation by hybridoma.
Epitope mapping has become more and more vital in both vaccine and antibody drug development. Knowledge on epitope of an antibody will largely facilitate your drug design and patent application. Based on our advanced peptide library platform, GenScript provides a comprehensive series of Epitope Mapping Services (Cat. No: SC1500) to fulfill customers' demand.
Knowing the sequence of your monoclonal antibody is not only the first step towards antibody engineering and function optimization, but also critical for patent application. GenScript's advanced technology offers fast and professional sequencing services for your hard-won monoclonal antibodies. We are capable of sequencing most antibodies with customer-selected specifications (see below for a list of different specifications). You can rely on our ISO 9001 certified Antibody Sequencing Service (Cat. No.: SC1408) to speed and facilitate your patent application, database banking, and therapeutic antibody development.
Top 3 pitfalls to avoid when sequencing antibodies
CRISPR/Cas9 genome editing has become widely used due to its simplicity and versatility, and CRISPR technology has been adapted for diverse applications aside from genome editing. As a leader in gene synthesis and genome editing, and through our partnership with Feng Zhang’s laboratory at the Broad Institute of MIT and Harvard*, GenScript offers validated CRISPR products, services and resources to help you harness the power of CRISPR genome editing for your research. Learn more about our CRISPR services – including gRNA database, CRISPR plasmids for genome editing, SAM constructs for Cas9-based activation of endogenous gene transcription, GeCKO and SAM libraries, mammalian cell line genome editing and microbial genome editing.
As a pioneer and leader in gene synthesis technology, and the largest gene synthesis supplier in the US, GenScript has completed over 600,000 genes synthesis projects for scientists around the world. Gene synthesis enables powerful solutions for molecular cloning, creating fusion proteins, or achieving sufficiently high protein expression levels. GenScript offers gene synthesis services with complete customizability and considerable savings of time and money compared to traditional molecular cloning using recombinant DNA technology.
Our experienced Ph.D.-level project managers ensure that you receive professional service tailored to your needs and on-time delivery of ISO 9001:2008 certified products with 100% sequence accuracy guaranteed.
|Gene Length||Starting Price *||Starting Turnaround Time (business days)||Minimum Order Size||Codon Optimization|
|Custom Gene Synthesis Cat No. SC1010||≤ 8kb||Starts at $0.35/bp or minimum charge $159/gene||8||No minimum||Optional (complimentary)|
|GenBrick™ Synthesis Cat No. SC1584||> 8 kb||Starts at $0.45/bp||23||No minimum||Optional (complimentary)|
|Rush Gene Synthesis Cat No. SC1575||≤ 2 kb||Request a quote||4||No minimum||Optional (complimentary)|
|Fast Gene Synthesis Cat No. SC1619||≤ 5 kb||Request a quote||7||No minimum||Optional (complimentary)|
|GenPlus™ HT Gene Synthesis Cat No. SC 1645||≤10 kb||Starts at $0.23/bp or $99/gene for orders ≥ 25 genes.||10-12||25 genes||Optional (complimentary)|
|GenPlus™ Economy Gene Synthesis Cat No. SC1681||≤10 kb||$0.23/bp||15||No minimum||Required (complimentary)|
Advantages of Custom Gene Synthesis
Our BacPower™ Guaranteed Bacterial protein expression packages offer a comprehensive panel of options for your target protein production in an E. coli system with guaranteed protein amounts, purities, endotoxin levels and the option of producing tag-free proteins as well, to meet a variety of your research needs. You simply provide protein sequence and we will deliver the protein (tagged or tag-free) at the amount and purity that you specified. All BacPower™ guaranteed bacterial protein expression packages start with our proprietary codon optimization service, followed by gene synthesis and sub cloning, all the way through protein expression and purification using our bacterial protein expression system. If your target recombinant protein becomes insoluble in the inclusion body fraction, FoldArt™, our proprietary protein refolding technology will be applied to solubilize the protein at no extra cost to you. This service is also ideal for the production of high quality protein antigens for antibody production. In case we fail on either the amount or purity as specified, there is no charge to you whatsoever.
Place your order today to experience unprecedented, high quality services yet competitive pricing, offered with our all of our Bacterial protein expression packages.
BacPower™ Guaranteed Bacterial protein Expression packages with unprecedented assurances
As the largest gene synthesis and comprehensive molecular biology service provider in the USA with proven capability and reliability, GenScript now expands DNA sequencing services in North America to offer our customers high speed and superior quality services. The Sanger DNA sequencing services is performed by our professionally trained staff on our state-of-the-art instrument and analyzed with advanced bioinformatics tools.
4 µg of lyophilized plasmid containing your gene insert
Sequence chromatograms covering your gene (electronic)
Construct map for the plasmid (electronic)
Quality assurance certificate
GenScript's BacuVance™ baculovirus expression system was developed by our in-house team of scientists for virus production and expression of recombinant proteins from baculovirus-infected insect cells. As the largest gene synthesis supplier in the U.S., GenScript can provide you with free, advanced codon optimization specific to Sf9, Sf21, Hi-5, and S2 insect cell lines, to further enhance the expression of your protein.
There are several advantages of using baculovirus expression system over E. coli system, such as improved solubility, ability to incorporate post-translational modifications, and higher yields for secreted proteins. For customers who prefer a eukaryotic expression system, are exploring lower-cost alternatives to mammalian expression system but do not want to compromise on the overall quality of their recombinant protein, we strongly recommend considering our BacuVance™ baculovirus expression system. Using our baculovirus expression system we can establish the best possible protocol for your custom project and then scale up to get the highest expression of your target protein. Alternatively, if you are looking for a fail-safe option for insect protein production at a competitive price, visit the InsectPower™ guaranteed protein page to learn more.
Transient Expression Of Recombinant Protein & Antibody
GenScript transient mammalian expression service offers fast production and delivery of high quality recombinant proteins and monoclonal antibodies at competitive prices, from pilot scale to guaranteed production, up to gram quantities.
Stable Cell Line Development
GenScript comprehensive stable cell line development capabilities seamlessly integrate all steps of stable cell line generation, delivering high quality stable clones of your choice. Guaranteed, gram level GS expression cell line delivered in just 4 months.
GenScript offers upstream and downstream bioprocess services that include cell culture condition improvement, optimization of feeding strategies, scale-up to bioreactor, purification process development, protein modification process development and analytical assay development.
Use our GLP-compliant bioprocess services for your recombinant protein and therapeutic antibody needs for in vivo studies. Smoothly integrate results for manufacturing and regulatory application. Learn More
DNA Oligo synthesis from $0.39
RNA Oligo synthesis from $5
GenScript provides high-quality peptides with a 95% peptide synthesis success rate, well above the industry standard of 75%. We are committed to Total Quality Management (TQM) to ensure our customers' complete satisfaction. TQM includes MS and HPLC analyses performed after the completion of synthesis, purification, and QC (quality control) steps. These analyses are followed by independent QA (quality assurance) procedures, which double guarantees the highest quality possible for every delivered peptide.
Peptide Synthesis Capacity and Capability
Our custom peptides are synthesized in a 20,000 square foot state-of-the-art facility with peptide synthesizers, HPLCs, and mass spectrometers capable of producing over 6000 peptides per month. Other features of our capacity and capability include:
GenScript plasmid DNA preparation services cover individual constructs for research labs as well as large scale manufacturing for biotech and pharmaceutical companies. GenScript’s high quality plasmids will help you achieve highly efficient cell transfection, DNA vaccination, antibody production, and other preclinical studies.
Site-directed mutagenesis is central to molecular biology, facilitating research in a variety of fields, including studies of gene regulatory elements, DNA-protein interaction, protein structure/function, enzyme active sites, and novel proteins. GenScript's site-directed mutagenesis services, powered by sophisticated gene synthesis technology and high performance GS polymerase, include point mutations, deletions, and insertions. They can be customized to fit any scientific applications.
Targeting vector construction is a prerequisite for the generation of genetically engineered animals. GenScript's advanced molecular biology platform has provided thousands of targeting vectors to customers worldwide. These targeting vectors have been successfully used in traditional and conditional knock-out, knock-in, and many other genetic modification projects.
Biomolecular Interaction Analysis Services
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