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Genomix4Life

Baronissi, IT

Genomix4Life is an innovative start-up, spin-off of the University of Salerno.

We provide a wide range of structural and functional genomics and bioinformatics services. Whether you are interested in application of these technologies in medicine, forensics, animal husbandry or veterinary, agrifood or any research and industrial area, we are the right choice to guarantee a successful answer to your questions.

The Company holds an NGS facility, providing cutting-edge technologies for high-throughput sequencing of nucleic acids (Illumina HiSeq1500, Genome Analyzer IIx and MiSeq, Life Technologies Ion Torrent PGM), a microarray lab equipped with Illumina BeadXpress Reader and iScan instrumentation and a bioinformatics core with access to high computational power (latest generation processors and a large on-line storage for genomics data).

Our instruments:
SEQUENCERS
- Illumina Nextseq 500: provides the flexible... Show more »

Genomix4Life is an innovative start-up, spin-off of the University of Salerno.

We provide a wide range of structural and functional genomics and bioinformatics services. Whether you are interested in application of these technologies in medicine, forensics, animal husbandry or veterinary, agrifood or any research and industrial area, we are the right choice to guarantee a successful answer to your questions.

The Company holds an NGS facility, providing cutting-edge technologies for high-throughput sequencing of nucleic acids (Illumina HiSeq1500, Genome Analyzer IIx and MiSeq, Life Technologies Ion Torrent PGM), a microarray lab equipped with Illumina BeadXpress Reader and iScan instrumentation and a bioinformatics core with access to high computational power (latest generation processors and a large on-line storage for genomics data).

Our instruments:
SEQUENCERS
- Illumina Nextseq 500: provides the flexible power and simplicity you need to analyze a wide range of applications. Data are generated in about 12 hours for a 75 cycles sequencing run and less than 30 hours for 150 cycles paired-end run.

  • Illumina Hiseq 2500: the first Illumina sequencing system allowing two run modes (100bp paired-end reads in a standard high output run and 150bp paired-end reads for rapid run) on one or two flowcells simultaneously, enabling a flexible and scalable platform to study genomes. It allows the economic availability of multiple gigabases of data from several millions of templates (60Gb from rapid run and 300Gb from single run)

  • Illumina MiSeq: a sequencer recommended for small projects, to produce sequences up to 250nt long, since it allows to generate up to 12.5 GB of data in a 2 x 250 paired-end run.

  • Life technologies Ion Torrent PGM: a sequencing platform that is ideally suited as an affordable alternative for sequencing of small genomes or targeted regions of larger genomes. Ion Torrent, similar to pyrosequencing, is able to sequence from 400,000 to 6 million reads in 2-4 hours.

MICROARRAY READERS
- Illumina BeadXpress: a platform for DNA genotyping that allows the simultaneous analysis of up to 384 SNPs for each sample by using VeraCode holographic micro-bead technology. This technology is ideal for large-scale genotyping studies and in vitro diagnostic.

  • Illumina iScan: uses high-performance lasers, optics and detection system, resulting in reduced scan times without sacrificing data quality and reproducibility. With a high sensitivity, low limit of detection, and broad dynamic range, this system produces exceptional data quality.

OTHER INSTRUMENTS
- Bioruptor NGS: This DNA fragmentation technology is proven as an optimal shearing device for library preparation by providing good sample yields, fragment size, and consistency, which are all essential parameters for Next-Generation Sequencing workflows.

  • Agilent MX3005P Real Time PCR: The logical step after a genome-wide approach is to validate with another technology the candidate genes that showed differential expression. The laboratory gives the possibility to do such validation by Real-Time qPCR.

  • Nanodrop 2000c: Nucleic acids quality control is carried out by spectrophotometric analysis using the Nanodrop 2000, which allows to determine 260/280 and 260/230 ratios using only 1ul of sample.

  • Qubit 2.0 Fluorometer: Qubit is a fluorometer that enables the quantification of RNA and DNA samples in an accurate manner, using specific fluorophores for RNA and for dsDNA.

  • Agilent 2100 Bioanalyzer: This system provides sizing, quantitation and quality control of nucleic acids, using a microfluidics technology to analyze the profiles of RNA and DNA of up to 12kb. It is used to verify the integrity of RNA samples and for the quality control of sequencing libraries.

Certifications:
- ISO 9001:2008 Quality Management System
- OHSAS 18001:2007
- Illumina certified service provider (CSPro)
- Ion Torrent certified service provider (CSPro)

Standard shipping requests:
- RNA/DNA samples should be suspended either in nuclease-free water or in TE
Ship samples in 0,5-2ml low retention tubes, marked at the top and sides with a clear and permanent identification code.
- Alternatively, libraries can be sent in a 96-well sealed plate together with a excel spread-sheet clearly reporting the plate content. Send RNA samples in a cold pack (e.g. dry ice.) while DNA samples in a cold pack (e.g. Blue ice)

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