Located at Georgetown University Medical Center, we provide services for various high throughput experiments including gene expression profiling, SNP genotyping, CNV analysis, DNA methylation, microRNA expression profiling, siRNA screening, DNA/RNA isolation, RNA quality assessment, DNA plating and assay preparation, DNA sequencing, and fragment analysis.
GESR uses state-of-the-art instruments and assays to provide these services. These include Affymetrix Microarray System, Agilent Microarray System, Applied Biosystems TaqMan assays, Illumina BeadXpress, Qiagen PyromarkMD, Qiagen Qiacube, Agilent Bioanalyzer, Beckman Multimek NXP Robotic Liquid Handling System, and Applied Biosystems Sequence Analyzer.
In addition, GESR houses several user-operated specialized instruments for which the shared resource personnel provide technical support and training.
Genomics & Epigenomics Shared Resource (GESR) has not listed any services.
Agilent CGH array Full Service (sample preparation, labeling, hybridization, scanning, and data analysis).
PanCancer Gene Expression Panels. Human and Mouse panels including 770 genes each comprised of essential cancer pathway and driver genes and up to 40 internal reference genes.Compatible with FFPE.
Copy Number Anaysis-full service:
Genome-Wide Human SNP Array 6.0
3' Gene Expression Analysis-full service:
Arrays including: GeneChip Human Genome U133 Plus 2.0, GeneChipMouse Genome 430A2.0, and GeneChip Rat Genome 230 2.0
Whole-Transcript Expression Analysis-full service:
Arrays including Human Exon 1.0 ST Array & Gene1.0 Array for Human, Mouse and Rat.
miRNA Analysis-full service:
GeneChip miRNA 2.0 array
Agilent CGH array Full Service (sample preparation, labeling, hybridization, scanning, and data analysis).
Agilent array scan (hybridization, scanning, and data analysis)
Agilent array scan (hybridization, scanning, and data analysis)
Agilent Gene Expression Full Service (sample preparation, labeling, hybridization, scanning, and data analysis).
Arrays include 8x60K or 4x44K Human Gene Expression Microarrays
Agilent Bioanalyzer 2100
Agilent Human miRNA array:
Full Service - (sample preparation, labeling, hybridization, scanning, and data analysis).
Copy Number Anaysis-full service:
Genome-Wide Human SNP Array 6.0 $450/sample (chip purchased separately)
DNA/RNA Isolation
Plating and Assay preparation:
Reformat 96-well plates into 384-well plate
Reformat 96 DNA samples in tubes to 96-well deep (plate, OD and normalize)
Illumina NextSeq 550 Next Generation Sequencing
WGS, WES, RNAseq, metagenomic sequencing, targeted sequencing
Illumina
Illumina Infinium Human MethylationEPIC BeadChip
ABI - SNP genotyping by TaqMan real-time PCR
miRNA analysis by TaqMan Low Density Array (TLDA), includes microRNA array, RT-PCR, and PCR reaction
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