1345075202000

Genomics Core Facility

Charlottesville, Virginia, US

The GCF provides second generation genome sequencing service to all members of the scientific community. Using the 454 Life Sciences/Roche Genome Sequencer FLX, we facilitate data collection and analysis.

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Roche 454 Sequencing
Roche 454 next generation sequencing
Price on request

Long single reads
- Approximately 400 nt of HQ pass for the vast majority of shotgun libraries
- Yields data for de novo assemblies of virtually any genomic―nuclear/organellar―samples
- Enables analysis of transcriptomes with fine resolution

3-, 8- or 20kb paired end libraries
- Provide positional information for... Show more »

Long single reads
- Approximately 400 nt of HQ pass for the vast majority of shotgun libraries
- Yields data for de novo assemblies of virtually any genomic―nuclear/organellar―samples
- Enables analysis of transcriptomes with fine resolution

3-, 8- or 20kb paired end libraries
- Provide positional information for scaffolding genomes and ordering contigs/existing assemblies
- Useful for complex genomes that are heavy with repeat motifs or that undergo frequent rearrangement

Sequence targeted amplicons
- Examine variation at multiple levels or organization via standard PCR
- Primers contain 454 process-specific sequence flanking the gene-specific regions
- Analyze hundreds of samples in a single run

Multiplexed analysis
- Employ a combination of small multiplex identifier (MID) nucleotide sequence tags and physical separation during sequencing to maximize unique samples and retain input identity

NimbleGen sequence capture arrays

Supported software suite for data analysis
- Each customer receives the most current GS software package
- GS Assembler
- GS Reference Mapper
- GS Amplicon Variant Analyzer
- Intuitive graphic or command line interfaces simplify the analysis pipeline

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Roche 454 NGS Data Analysis
Roche 454 next generation sequencing data analysis
Price on request

Supported software suite for data analysis
- Each customer receives the most current GS software package
- GS Assembler
- GS Reference Mapper
- GS Amplicon Variant Analyzer
- Intuitive graphic or command line interfaces simplify the analysis pipeline

Supported software suite for data analysis
- Each customer receives the most current GS software package
- GS Assembler
- GS Reference Mapper
- GS Amplicon Variant Analyzer
- Intuitive graphic or command line interfaces simplify the analysis pipeline

« Show less
Sequencing of PCR Amplicons
Price on request

Sequence targeted amplicons
- Examine variation at multiple levels or organization via standard PCR
- Primers contain 454 process-specific sequence flanking the gene-specific regions
- Analyze hundreds of samples in a single run

Sequence targeted amplicons
- Examine variation at multiple levels or organization via standard PCR
- Primers contain 454 process-specific sequence flanking the gene-specific regions
- Analyze hundreds of samples in a single run

« Show less
Biology
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Biochemistry & Molecular Biology
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Nucleic Acid Services
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Nucleic Acid Analysis
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Nucleic Acid Data Analysis
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Sequencing Data Analysis
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DNA Services
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DNA Sequencing
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Next Generation Sequencing (NGS)
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