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Genomic Sciences Laboratory

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Raleigh, North Carolina, US

About Genomic Sciences Laboratory

Divison of: NC State University

Established in 2000, the mission of the Genomic Sciences Laboratory (GSL) is to enable discovery in the biological sciences by providing researchers and students with the necessary cutting-edge equipment and infrastructure required to conduct advanced genomics research. The GSL operates as a solution... Show more »

Established in 2000, the mission of the Genomic Sciences Laboratory (GSL) is to enable discovery in the biological sciences by providing researchers and students with the necessary cutting-edge equipment and infrastructure required to conduct advanced genomics research. The GSL operates as a solution center and provides NC State researchers, collaborators, and the broader scientific community with access to high-throughput DNA sequencing, traditional Sanger sequencing and genotyping, and additional equipment services for functional genomics research. The GSL is administered by the Office of Research and Innovation at NC State and operates on a fee-recovery basis. 

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Our Services (10)


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RNA Extraction and Purification

Price on request

RNA Isolation from Tissues

The GSL offers RNA extraction services (tissue) when combined with NGS Library Prep & Sequencing. Our facility is not able to process blood or pathogenic samples that may pose risk to human health - we are a BSL-1 level facility with University student access.

RNA Isolation from Tissues

The GSL offers RNA extraction services (tissue) when combined with NGS Library Prep & Sequencing. Our facility is not able to process blood or pathogenic samples that may pose risk to human health - we are a BSL-1 level facility with University student access.

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STR Genotyping

Short tandem repeat genotyping
Price on request

Microsatellite/STR/Fragment Analysis Genotyping

The GSL offers capillary runs for user-prepared Fragment Analysis on our ABI 3730xl sequencer. 

Microsatellite/STR/Fragment Analysis Genotyping

The GSL offers capillary runs for user-prepared Fragment Analysis on our ABI 3730xl sequencer. 

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Sanger Sequencing

Price on request

The GSL offers single tube or 96-well plate Sanger Sequencing (ABI 3730xl) at difference service levels. For more information about submitting samples, please see our sequencing... Show more »

The GSL offers single tube or 96-well plate Sanger Sequencing (ABI 3730xl) at difference service levels. For more information about submitting samples, please see our sequencing guidelines.

Platinum Service - PCR amplicon cleanup by Exosap, Dye terminator reaction and cleanup, and capillary sequencing run. User provides primer and templates in separate plates or tubes.

Gold Service - Dye terminator reaction and cleanup, capillary sequencing run. User must provide primer and appropriate DNA template amount in same tube (see guidelines).

Bronze Service - User does their own Big Dye Terminator reactions. These are simply bead cleaned (excess Dye terminator removal) and run on the capillary sequencer.

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DNA Sequencing

Price on request

The GSL offers a variety of DNA sequencing services, including Sanger DNA sequencing, microsatellite/STR genotyping (user made plate runs), Next Generation DNA sequencing on Illumina MiSeq, NextSeq, and NovaSeq 6000, as well as Pacific Biosciences Sequel platforms.

 

The GSL offers a variety of DNA sequencing services, including Sanger DNA sequencing, microsatellite/STR genotyping (user made plate runs), Next Generation DNA sequencing on Illumina MiSeq, NextSeq, and NovaSeq 6000, as well as Pacific Biosciences Sequel platforms.

 

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Illumina MiSeq Illumina NextSeq 500 sequencing system PacBio Sequel System Illumina NovaSeq 6000 NGS Library Preparation

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Next Generation Sequencing (NGS)

Price on request

The GSL offers the following Illumina and PacBio NGS Library Preparation and Sequencing services:

 

Illumina WGS DNA (Truseq or Nextera DNA Flex) Library Prep

NEB Ultra Directional RNA Library Prep (polyA enrichment or ribodepletion)

Illumina small RNA (Truseq) Library Prep

16S Amplicon... Show more »

The GSL offers the following Illumina and PacBio NGS Library Preparation and Sequencing services:

 

Illumina WGS DNA (Truseq or Nextera DNA Flex) Library Prep

NEB Ultra Directional RNA Library Prep (polyA enrichment or ribodepletion)

Illumina small RNA (Truseq) Library Prep

16S Amplicon Library Prep (48 or 96 sample block, Nextera workflow)

Hi-C Library Prep (Phase Genomics)

PacBio SMRTbell Library Prep (up to 20 kb fragments)

PacBio Iso-Seq RNA sequencing Library Prep

 

The GSL houses the following NextGen DNA sequencers:

 

Illumina MiSeq system

Illumina NextSeq 500 System

Illumina NovaSeq 6000 System

PacBio Sequel System

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NovaSeq Sequencing

Price on request

The GSL offers the following sequencing options for the Illumina NovaSeq 6000 system:

 

Single XP lane options (requires longer turnaround to pair with other jobs):

 

SP 100 SE XP lane (~ 400 Million [M] clusters)

SP 150x2 PE XP lane (~ 400 M clusters)

S4... Show more »

The GSL offers the following sequencing options for the Illumina NovaSeq 6000 system:

 

Single XP lane options (requires longer turnaround to pair with other jobs):

 

SP 100 SE XP lane (~ 400 Million [M] clusters)

SP 150x2 PE XP lane (~ 400 M clusters)

S4 150x2 PE XP lane (~ 2.5 Billion [B] clusters, longer wait times to fill flow cell)

 

Full Flow Cell Options:

SP Flow Cell (2 XP lanes, 800 M clusters)- 50x2 PE/100 SE, 150x2 PE, or 250x2 PE

S1 Flow Cell (2 XP lanes, 1.6 B clusters) - 50x2 PE/100 SE, 100x2 PE, or 150x2 PE

S2 Flow Cell (2 XP lanes; 4.1 B clusters) - 50x2 PE/100 SE, 100x2 PE, or 150x2 PE

 

Note: Clusters are equivalent to number of reads or paired reads, depending on cycling settings (SE vs PE).

S4 Flow Cell (4 XP lanes; 10 B clusters) - 100x2 PE or 150x2 PE

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Illumina NovaSeq 6000 Illumina NovaSeq

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Illumina NextSeq Sequencing

Price on request

The GSL offers the following sequencing options for the Illumina NextSeq 500 system:

 

NextSeq High Output Flow Cell (400 M clusters)- 75 bp SE

NextSeq High Output Flow Cell (400 M clusters)- 75x2 PE or 150 SE

NextSeq High Output Flow Cell (400 M clusters)- 150x2... Show more »

The GSL offers the following sequencing options for the Illumina NextSeq 500 system:

 

NextSeq High Output Flow Cell (400 M clusters)- 75 bp SE

NextSeq High Output Flow Cell (400 M clusters)- 75x2 PE or 150 SE

NextSeq High Output Flow Cell (400 M clusters)- 150x2 PE

 

Note: Clusters are equivalent to number of reads or paired reads, depending on cycling settings (SE vs PE).

 

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Illumina NextSeq 500 sequencing system Illumina NextSeq

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Illumina MiSeq Sequencing

Price on request

The GSL offers the following sequencing options for the Illumina MiSeq system:

 

v2 250x2 PE Nano Flow Cell - (~1 M clusters)

v2 150x2 PE Flow Cell - (~12 M clusters)

v2 250x2 PE Flow Cell - (~12 M clusters)

v3 75x2 PE or 150 SE Flow Cell - (~22-24 M clusters)

v3... Show more »

The GSL offers the following sequencing options for the Illumina MiSeq system:

 

v2 250x2 PE Nano Flow Cell - (~1 M clusters)

v2 150x2 PE Flow Cell - (~12 M clusters)

v2 250x2 PE Flow Cell - (~12 M clusters)

v3 75x2 PE or 150 SE Flow Cell - (~22-24 M clusters)

v3 300x2 PE Flow Cell - (~22-24 M clusters)

 

Note: Clusters are equivalent to number of reads or paired reads, depending on cycling settings (SE vs PE).

 

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SMRT Sequencing

Single Molecule Real-Time Sequencing
Price on request

The GSL offers the following Pacific Biosciences SMRT (Single Molecule, Real Time) Library Prep and Sequencing Services:

 

PacBio SMRTbell Library Prep (up to 20 kb fragments)

PacBio Iso-Seq RNA sequencing Library Prep

PacBio Microbial Indexing Library Prep

 

Sequencing is... Show more »

The GSL offers the following Pacific Biosciences SMRT (Single Molecule, Real Time) Library Prep and Sequencing Services:

 

PacBio SMRTbell Library Prep (up to 20 kb fragments)

PacBio Iso-Seq RNA sequencing Library Prep

PacBio Microbial Indexing Library Prep

 

Sequencing is performed on an in-house PacBio Sequel instrument, with tiered (discounted) pricing available for 8-cell, 4-cell, and 2-cell blocks.

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PacBio Sequel System Pacific Biosciences

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DNA Quantification

Price on request

The GSL offers Agilent nucleic acid quantification and quality assessement (QC) either a la carte or with current sequencing projects. The following quantification options are available:

 

Tapestation High Molecular Weight DNA assay (up to 60 kb)

Tapestation DNA Assay (D1000 or... Show more »

The GSL offers Agilent nucleic acid quantification and quality assessement (QC) either a la carte or with current sequencing projects. The following quantification options are available:

 

Tapestation High Molecular Weight DNA assay (up to 60 kb)

Tapestation DNA Assay (D1000 or D5000)

Tapestation High Sensitivity Assay (D1000 or D5000)

Bioanalyzer RNA Assay (Pico or Nano options)

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Agilent Bioanalyzer Agilent TapeStation Agilent

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David Baltzegar

Director

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