1310477538000

Genome Technology Access Center (GTAC)

St. Louis, Missouri, US

The Genome Technology Access Center (GTAC) provides DNA/RNA analysis resources for researchers at Washington University and beyond.

We currently provide NextGen sequencing (4 illumina HiSeq 2500, 2 Illumina MiSeq), microarray (all formats), Fluidigm Biomark (qPCR, amplicon sequencing, etc). We provide analysis services with all these technologies.

Genome Technology Access Center (GTAC) has not listed any services.

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DNA/RNA Analysis and Quality Control
Price on request
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Agilent 2100 Bioanalyzer
Biomarker Analysis
Price on request
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Gene expression profiling
16S rRNA Sequencing
Starting at $15.00 per sample

MVRSION was recently developed by our facility for the purpose of using 16S to define metabiomic bacterial populations from human, animal, or environmental sources.

The suite is a methodology that simultaneously leverages all nine 16S hyper-variable regions to characterize either host or environmental metagenomes. We use a... Show more »

MVRSION was recently developed by our facility for the purpose of using 16S to define metabiomic bacterial populations from human, animal, or environmental sources.

The suite is a methodology that simultaneously leverages all nine 16S hyper-variable regions to characterize either host or environmental metagenomes. We use a Fluidigm Biomark to economically prep the nine regions. These are all sequenced and analyzed in parallel, using the MVRSION computational protocol. The underlying database is based off of silva release 123 which we curated to remove all sequences which do not contain a bacterial genus/species. MVRSION will call a species or, if no species is found, a genus is called from a separate genus database. We will soon release a version of the database which includes curated versions of both silva and rdp databases.  
 
We have prepared a manuscript describing MVERSION which has been submitted to Nature Methods. In preparation for this we ran a wide variety of sample types, comparing MVRSION with the most commonly used, “gold standard" 16S protocol, QIIME. These included pure, defined samples in both equimolar and non-equimolar ratios; defined fecal specimens and those derived from human skin (nose, armpit, arm and groin) partially defined by culture. We generated data demonstrating the striking improvement in specificity and positive-predictive-value that MVRSION shows over QIIME. MVRSION is capable of species-level calls at four orders of magnitude abundance. I believe we can provide you a clearer profile of your specimens more economically than anything that is out there. Please contact me if you would like a short web presentation!

Libraries
Fluidigm 48X48 16s format: $1619 (48 samples) = ~$34/library
Fluidigm 192X24 16s format: $2857.90 (192 samples) = ~$15/library

Sequencing
MiSeq (12-15M reads/lane – we recommend >200K/sample) - $1560/lane
HiSeq 3000 2X150 (~360M reads/lane) - $3995/lane.

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Fluidigm Biomark HD
Bacteria
16S
Gene Copy Number Analysis
Price on request
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Illumina BeadArray Reader
Illumina Human Core
Illumina Human Omni 2.5
Illumina Human Omni 2.5 Exome
Illumina Human Omni 5
Oncology
Targeted Sequencing
Price on request

Either clinical or research samples. Either by hybridization or amplicon. For amplicon our Fludigm Access Array system, we can simultaneously enrich 480 targets of interest from 48 samples at a time. When used with the Access Array barcode library, each library is uniquely tagged, so up to 384 samples can be pooled and sequenced in a single multiplex sequencing run.

Either clinical or research samples. Either by hybridization or amplicon. For amplicon our Fludigm Access Array system, we can simultaneously enrich 480 targets of interest from 48 samples at a time. When used with the Access Array barcode library, each library is uniquely tagged, so up to 384 samples can be pooled and sequenced in a single multiplex sequencing run.

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Fluidigm Access Array System
Agilent SureSelect
Illumina Sequencing
Price on request

MiSeq
HiSeq 2500
HiSeq 3000
NovaSeq

MiSeq
HiSeq 2500
HiSeq 3000
NovaSeq

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Illumina HiSeq 2500 sequencing system
Illumina MiSeq sequencing system
microgram
Illumina Novaseq
RNA Sequencing
Price on request

miRNA or mRNA. We can handle samples down to 500pg. Libraries prepared by poly-A purification, ribo-depletion, the Clontech SMARTer system, Sigma Seqplex or Illumina TruSeq Small RNA Library Preparation.

miRNA or mRNA. We can handle samples down to 500pg. Libraries prepared by poly-A purification, ribo-depletion, the Clontech SMARTer system, Sigma Seqplex or Illumina TruSeq Small RNA Library Preparation.

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Single Nucleotide Polymorphism (SNP) Genotyping
Price on request
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Whole Exome Sequencing (WES)
Starting at $653.00 per sample

Agilent Exomes. Please note price varies depending on the number of samples, the library prep, and sequencing coverage.

Agilent Exomes. Please note price varies depending on the number of samples, the library prep, and sequencing coverage.

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Illumina HiSeq 2500 sequencing system
Illumina HiSeq 3000
Agilent SureSelect
Whole Genome Sequencing
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Genotyping Services
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DNA Sequencing
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Targeted Gene Sequencing
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Biology
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Next Generation Sequencing (NGS)
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Exome Sequencing Services
Price on request
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DNA
Price on request
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RNA
Price on request
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Pharmacology & Toxicology
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Bioanalysis
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Biomarkers
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Biochemistry & Molecular Biology
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Nucleic Acid Services
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January 21, 2016

Positive review received for RNA Sequencing:

"The service provided by GTAC was exceptional! The work was done in a timely manner and the deliverables went above and beyond my expectations. I look forward to working with them again very soon."

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