1310477538000

Genome Technology Access Center (GTAC)

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St. Louis, Missouri, US

About Genome Technology Access Center (GTAC)

The Genome Technology Access Center (GTAC) provides DNA/RNA analysis resources for researchers at Washington University and beyond.

We currently provide NextGen sequencing (4 illumina HiSeq 2500, 2 Illumina MiSeq), microarray (all formats), Fluidigm Biomark (qPCR, amplicon sequencing, etc). We provide analysis services with all these technologies.

Our Services (24)


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PCR

Price on request
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Fluidigm Biomark HD

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DNA/RNA Analysis and Quality Control

Price on request
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Agilent Bioanalyzer

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Biomarker Analysis

Price on request
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Gene expression profiling

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16S rRNA Sequencing

Starting at $15.00 per sample

MVRSION was recently developed by our facility for the purpose of using 16S to define metabiomic bacterial populations from human, animal, or environmental sources.

The suite is a methodology that simultaneously leverages all nine 16S hyper-variable regions to characterize either host or environmental metagenomes. We use a... Show more »

MVRSION was recently developed by our facility for the purpose of using 16S to define metabiomic bacterial populations from human, animal, or environmental sources.

The suite is a methodology that simultaneously leverages all nine 16S hyper-variable regions to characterize either host or environmental metagenomes. We use a Fluidigm Biomark to economically prep the nine regions. These are all sequenced and analyzed in parallel, using the MVRSION computational protocol. The underlying database is based off of silva release 123 which we curated to remove all sequences which do not contain a bacterial genus/species. MVRSION will call a species or, if no species is found, a genus is called from a separate genus database. We will soon release a version of the database which includes curated versions of both silva and rdp databases.  
 
We have prepared a manuscript describing MVERSION which has been submitted to Nature Methods. In preparation for this we ran a wide variety of sample types, comparing MVRSION with the most commonly used, “gold standard" 16S protocol, QIIME. These included pure, defined samples in both equimolar and non-equimolar ratios; defined fecal specimens and those derived from human skin (nose, armpit, arm and groin) partially defined by culture. We generated data demonstrating the striking improvement in specificity and positive-predictive-value that MVRSION shows over QIIME. MVRSION is capable of species-level calls at four orders of magnitude abundance. I believe we can provide you a clearer profile of your specimens more economically than anything that is out there. Please contact me if you would like a short web presentation!

Libraries
Fluidigm 48X48 16s format: $1619 (48 samples) = ~$34/library
Fluidigm 192X24 16s format: $2857.90 (192 samples) = ~$15/library

Sequencing
MiSeq (12-15M reads/lane – we recommend >200K/sample) - $1560/lane
HiSeq 3000 2X150 (~360M reads/lane) - $3995/lane.

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Fluidigm Biomark HD Bacteria

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Gene Copy Number Analysis

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Illumina BeadArray Reader Illumina Human Core Illumina Human Omni 2.5 Illumina Human Omni 2.5 Exome Illumina Human Omni 5 Oncology Show 6 more tags Show less

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Targeted Sequencing

Price on request

Either clinical or research samples. Either by hybridization or amplicon. For amplicon our Fludigm Access Array system, we can simultaneously enrich 480 targets of interest from 48 samples at a time. When used with the Access Array barcode library, each library is uniquely tagged, so up to 384 samples can be pooled and sequenced in a single multiplex sequencing run.

Either clinical or research samples. Either by hybridization or amplicon. For amplicon our Fludigm Access Array system, we can simultaneously enrich 480 targets of interest from 48 samples at a time. When used with the Access Array barcode library, each library is uniquely tagged, so up to 384 samples can be pooled and sequenced in a single multiplex sequencing run.

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Fluidigm Access Array Agilent SureSelect

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Illumina Sequencing

Price on request

MiSeq
HiSeq 2500
HiSeq 3000
NovaSeq

MiSeq
HiSeq 2500
HiSeq 3000
NovaSeq

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Illumina HiSeq 2500 Illumina MiSeq Illumina Novaseq

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RNA Sequencing

Price on request

miRNA or mRNA. We can handle samples down to 500pg. Libraries prepared by poly-A purification, ribo-depletion, the Clontech SMARTer system, Sigma Seqplex or Illumina TruSeq Small RNA Library Preparation.

miRNA or mRNA. We can handle samples down to 500pg. Libraries prepared by poly-A purification, ribo-depletion, the Clontech SMARTer system, Sigma Seqplex or Illumina TruSeq Small RNA Library Preparation.

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SNP Genotyping

Single Nucleotide Polymorphism Genotyping
Price on request
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Whole Exome Sequencing (WES)

Starting at $653.00 per sample

Agilent Exomes. Please note price varies depending on the number of samples, the library prep, and sequencing coverage.

Agilent Exomes. Please note price varies depending on the number of samples, the library prep, and sequencing coverage.

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Illumina HiSeq 2500 Illumina HiSeq 3000 Agilent SureSelect

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Whole Genome Sequencing

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Genotyping and Gene Expression Assays

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Biochemistry & Molecular Biology

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Bioanalysis

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Next Generation Sequencing (NGS)

Price on request
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Targeted Gene Sequencing

Price on request
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DNA Services

Price on request
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Nucleic Acid Services

Price on request
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Pharmacology & Toxicology

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Exome Sequencing Services

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Biology

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RNA Services

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DNA Sequencing

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Biomarkers

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Seth Crosby

Director, Partnerships
  • Positive review for RNA Sequencing:

    January 2016

    "The service provided by GTAC was exceptional! The work was done in a timely manner and the deliverables went above and beyond my expectations. I look forward to working with them again very soon."

Genome Technology Access Center (GTAC) has not received any endorsements.