Over the last few weeks, [email protected] has been receiving numerous requests as to our status and availability to accept samples. As our employee’s health and well-being are paramount, we were initially only accepting COVID-19 related samples. However, our new alternate operations model will allow us to begin accepting any samples, including non-COVID, beginning Monday, April 27th for the workflows provided below. Please be assured that we continue to take all necessary health precautions for our staff during this time.
Full-service RNA and DNA library prep. This includes validatedSARS-CoV2 biotinylated baits.
Five Illumina NovaSeq 6000s
Illumina and Affymetrix genotyping arrays
Fluidigm high through-put, 1000s of reactions in a single run
Microarray-based detection of ~1300 proteins in serum/plasma, CSF, lysates, etc.
Genome visualization for detection of large (1kb-1MB) variants
We are accepting DNA or RNA as input materials for these platforms.
Research use only.
The Genome Technology Access Center (GTAC) has recently merged with the McDonnell Genome Institute (MGI). Together we provide a wide variety of DNA/RNA analysis resources for researchers at Washington University and beyond. We currently house:
We provide analysis services with all these technologies.
Nanostring nCounter® Analysis System.
Gene expression panels
• Custom Panels
• PanCancer Pathways kit
• GX Human Kinase Kit
• GX Human Immunology v2 Kit
• Human Cancer Reference Kit
• Human Inflammation Kit v2
• GX Mouse Immunology Kit
• Mouse Inflammation Kit v2
• Human Reference GX Kit
miRNA expression analysis
• Human miRNA
• Mouse miRNA
• Rat miRNA
• Fly miRNA
We are the Certified Provider for Bionano Genomics. Bionano systems provide essential genomic information no other technology can provide to help advance greater understanding of genome biology. Bionano’s next-generation mapping combines proprietary NanoChannel arrays with optical mapping to image extremely long, high-molecular-weight DNA in its most native state.
SOMAscan is a microarray-based assay which measures more than 1,300 protein analytes in serum, plasma or cerebrospinal fluid, urine or equally small amounts of a variety of other biological matrices, such a lysates. The assay offers an approximate dynamic range of 8 logs, quantifying proteins from picomolar to micromolar, with reproducibility of 4.6 median %CV for plasma. The SOMAscan proteomic assay is enabled by protein-capture SOMAmers® (Slow Off-rate Modified Aptamer) reagents. The SOMAscan assay measures native proteins in complex matrices by transforming each individual protein concentration into a corresponding SOMAmer reagent concentration, which is then quantified by standard DNA microarray. To date, the SOMAscan assay has been applied successfully to biomarker discovery and validation in many pharmaceutical research and development projects, diagnostics discovery and development projects, and academic research projects.
MVRSION was recently developed by our facility for the purpose of using 16S to define metabiomic bacterial populations from human, animal, or environmental sources.
The suite is a methodology that simultaneously leverages all nine 16S hyper-variable regions to characterize either host or environmental metagenomes. We use a Fluidigm Biomark to economically prep the nine regions. These are all sequenced and analyzed in parallel, using the MVRSION computational protocol. The underlying database is based off of silva release 123 which we curated to remove all sequences which do not contain a bacterial genus/species. MVRSION will call a species or, if no species is found, a genus is called from a separate genus database. We will soon release a version of the database which includes curated versions of both silva and rdp databases.
We have prepared a manuscript describing MVERSION which has been submitted to Nature Methods. In preparation for this we ran a wide variety of sample types, comparing MVRSION with the most commonly used, “gold standard" 16S protocol, QIIME. These included pure, defined samples in both equimolar and non-equimolar ratios; defined fecal specimens and those derived from human skin (nose, armpit, arm and groin) partially defined by culture. We generated data demonstrating the striking improvement in specificity and positive-predictive-value that MVRSION shows over QIIME. MVRSION is capable of species-level calls at four orders of magnitude abundance. I believe we can provide you a clearer profile of your specimens more economically than anything that is out there. Please contact me if you would like a short web presentation!
Fluidigm 48X48 16s format: $1619 (48 samples) = ~$34/library
Fluidigm 192X24 16s format: $2857.90 (192 samples) = ~$15/library
MiSeq (12-15M reads/lane – we recommend >200K/sample) - $1560/lane
HiSeq 3000 2X150 (~360M reads/lane) - $3995/lane.
Either clinical or research samples. Either by hybridization or amplicon. For amplicon our Fludigm Access Array system, we can simultaneously enrich 480 targets of interest from 48 samples at a time. When used with the Access Array barcode library, each library is uniquely tagged, so up to 384 samples can be pooled and sequenced in a single multiplex sequencing run.
miRNA or mRNA. We can handle samples down to 500pg. Libraries prepared by poly-A purification, ribo-depletion, the Clontech SMARTer system, Sigma Seqplex or Illumina TruSeq Small RNA Library Preparation.
Agilent Exomes. Please note price varies depending on the number of samples, the library prep, and sequencing coverage.
BioNano Mapping and 10X WGS Sequencing, Disk Allocation and Supernova Assemblies
Biochemistry & Molecular Biology Services
"The service provided by GTAC was exceptional! The work was done in a timely manner and the deliverables went above and beyond my expectations. I look forward to working with them again very soon."
[email protected] has not received any endorsements.