The Genome Engineering and IPSC Center (GEiC) was formed by the consolidation of two pre-existing cores, the Genome Engineering Center and the Induced Pluripotent Stem cell (iPSC) core, both established by the Department of Genetics in the past few years. These two Centers were established to facilitate functional genomic studies through the use of patient-derived iPSCs and the generation of modified cells and organisms using genome editing technologies. The recent merging of these Centers reflects the new opportunities afforded by uniting these powerful new technologies. The GEiC includes a consultative service whereby investigators are able to query experts in genomic engineering technologies and in generation and differentiation of patient-derived induced pluripotent stem cells (iPSCs).
Papers Citing Our Services
Genome Engineering and Stem Cell Technology Papers by Our Scientists
Custom iPSC Line Creation
CRISPR nucleases can be used to create iPSC lines with specific genomic modifications (as above). The capability to genetically incorporate (or correct) disease-causing point mutations in patient-derived iPSC lines will be invaluable for elucidating disease mechanisms through functional genomics, for identifying therapeutic agents and for the development of new cell-based therapies. The GEC has now been merged with the Induced Pluripotent Stem cell (iPSC) facility, which banks patient fibroblasts, generates iPSCs, and develops iPSC differentiation schemes. This allows for a seamless transition from patient derived iPSC generation to the engineering of modified iPSC lines. The GEP will have access to all the capabilities of this newly combined Center.
Donor design and creation services
To produce user-defined knockin mutations (e.g. incorporation of disease-causing point mutations), a donor substrate (either DNA fragment or oligonucleotide) must be co-delivered with the CRISPR nuclease and incorporated into the locus by homology directed repair. We offer donor plasmid and donor oligonucleotide design and validation services in addition to the requisite CRISPR nucleases.
CRISPR nuclease mRNA production that is ready for embryo injection
CRISPR nucleases have successfully been used to create novel mouse and rat transgenic models. Direct injection of mRNA encoding Cas9 and the gRNA into one-cell stage embryos results in rapid and efficient generation of model animals with a wide variety of modifications including gene deletions, point mutations, conditional knockouts, epitope and fluorescent reporter gene tagging and multiplex modifications. Although, AMRF investigators are primarily utilizing rodent models, most other organisms can be modified using CRISPR/Cas9 methods because the technology is species-agnostic.
Multiple backbones are available (e.g. separate Cas9 and gRNA backbone, all-in-one Cas9/gRNA backbone, all-in-one Cas9-GFP backbone, etc)
Genotyping services include assay design, targeted deep-sequencing, STR profiling, and zygosity analysis.
"Good communication and the order was completed in a timely fashion. Importantly, good quality of the results from genotyping of CRISPR edited cell pools using target-mediated deep sequencing!"
Genome Engineering and iPSC Center has not received any endorsements.