Lduom8idrhasgfttwh5a ivanfranko

Genetics and Evolution of Microbial Secondary metabolism

ic No Reviews Yet
ic No Completed Orders
Lviv, UA

About Genetics and Evolution of Microbial Secondary metabolism

We are experts in genetics of actinobacteria - bacteria with huge, GC-rich genomes producing vast array of industrially important natural products. We capitalize on over 30 years of experience of lab members in studies and genetic manipulations of various members of this class, including many industtrially... Show more »

We are experts in genetics of actinobacteria - bacteria with huge, GC-rich genomes producing vast array of industrially important natural products. We capitalize on over 30 years of experience of lab members in studies and genetic manipulations of various members of this class, including many industtrially important species producing such antibiotics as kanamycin, doxorubicin, erythromycin, moenomycin and teicoplanin. Area of our current research interest include:
- Genetic tools for actinobacteria
- Experimental evolution of secondary metabolism
- Biosynthesis of and resistance to antibiotics
- Actinobacterial genomics
- Evolutionary forces shaping unequal usage of codons within and across actinobacterial genomes

Show less

Our Services (16)


ic

Microbial Strain Development

Price on request

Strain improvement will be pursued (depending on project goals) in one of the following ways (or in combination):

  • selection of spontaneous overproducing variants
  • transposon mutagenesis
  • overexpression of pathway-specific or global regulatory genes
  • introduction in the genome of strain of interest of rpsL alleles known... Show more »

Strain improvement will be pursued (depending on project goals) in one of the following ways (or in combination):

  • selection of spontaneous overproducing variants
  • transposon mutagenesis
  • overexpression of pathway-specific or global regulatory genes
  • introduction in the genome of strain of interest of rpsL alleles known to increase antibiotic production

We have all necessary instrumentation for microbiological and molecular genetic work.

Our requirements:

  • initial level of production (mg/L in defined liquid medium)
  • method of antibiotic quantification (simple bioassay- or TLC- or colorimetry-based methods)
  • sequence of the gene cluster responsible for production of antibiotic of interest.

If the initial strain is wild type isolate (not selected/improved previously), we deliver its derivatives producing at least two times more antibiotic than the wild type. In most cases we expect to generate these derivatives within one year or less.

Show less

ic

Gene Cloning Systems for Actinomycetes

Price on request

We develop methods for introduction of replicative and integrative plasmid DNA into the cells of actinomycetes.

We offer expertise in four approaches:

  • electroporation
  • intergeneric conjugation
  • protoplast transformation
  • transformation of competent cells.

We first test the most efficient method (conjugation),... Show more »

We develop methods for introduction of replicative and integrative plasmid DNA into the cells of actinomycetes.

We offer expertise in four approaches:

  • electroporation
  • intergeneric conjugation
  • protoplast transformation
  • transformation of competent cells.

We first test the most efficient method (conjugation), and only proceed to other approaches if this is unsuccessful. Per customer request we can use a different experimental scheme.

We have a large suite of actinomycete vectors that span all major taxa of this order; we can also test plasmids provided by the customer; he(she) also may request to deliver specific genes. We require: strain with known genus affiliation.

Deliverables
We deliver step-by-step description of the gene transfer procedure and provide recombinant strains harboring plasmids that were successfully introduced into the strain. In most cases we deliver these materials within six months.

Show less

ic

Actinomycete Cell Culture

Price on request

We will develop a cultivation conditions for actinomycetes - for sporulation or production of antibiotics

We will develop a cultivation conditions for actinomycetes - for sporulation or production of antibiotics

Show less

ic

Microbiology

Price on request
Request a quote for more information about this service.

ic

Biology

Price on request
Request a quote for more information about this service.

ic

Electroporation

Price on request
Request a quote for more information about this service.

ic

Mutagenesis

Price on request
Request a quote for more information about this service.

ic

Bacterial Culture

Price on request
Request a quote for more information about this service.

ic

Transfection

Price on request
Request a quote for more information about this service.

ic

Nucleic Acid Services

Price on request
Request a quote for more information about this service.

ic

Biochemistry & Molecular Biology

Price on request
Request a quote for more information about this service.

ic

Cell and Tissue Culture

Price on request
Request a quote for more information about this service.

ic

Molecular Cloning

Price on request
Request a quote for more information about this service.

ic

Cells and Tissues

Price on request
Request a quote for more information about this service.

ic

Custom Gene Cloning

Price on request
Request a quote for more information about this service.

ic

DNA Services

Price on request
Request a quote for more information about this service.

Not finding what you're looking for?

Get info on this provider's capabilities without requesting a quote.
Missing Avatar

Bohdan Ostash

Professor

Genetics and Evolution of Microbial Secondary metabolism has not received any reviews.

Genetics and Evolution of Microbial Secondary metabolism has not received any endorsements.