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Genediscovery

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Memphis, Tennessee, US

About Genediscovery

Our lab's research is mainly focused on mutated gene discovery for disease models by using a new strategy which combines genetic mapping, genome resources, and high-throughput technologies such as microarray, capillary electrophoresis etc.

We offer full microarray service using Illumina platform or... Show more »

Our lab's research is mainly focused on mutated gene discovery for disease models by using a new strategy which combines genetic mapping, genome resources, and high-throughput technologies such as microarray, capillary electrophoresis etc.

We offer full microarray service using Illumina platform or Affymetrix platform. We also offer DNA analysis service using SpectruMedix system.

Recent Publications:

  • Sun S, Jiao Y, Wei W, Postlethwaite AE, Gu W, Sun D. 'Comparison of LAIR-1 genetic pathways in murine vs human internal organs'. Gene. 2014 Nov 15;552(1):140-5.
  • Huang Y, Zhu X, Wang L, Liu X, Lu L, Gu W, Jiao Y. 'Genome Wide Analysis of Sex Difference in Gene Expression Profiles of Bone Formations Using sfx Mice and BXD RI Strains'. Scientific World Journal. 2014;2014:584910.
  • Liu F, Shan B, Zhang R, Wang C, Gu W. 'Global standards for paediatric oncology nursing in low-to-middle income countries'. Lancet Oncol. 2014 Sep;15(10):e415.
  • Wang L, Jiao Y, Cao Y, Liu G, Wang Y, Gu W. 'Limitation of Number of Strains and Persistence of False Positive Loci in QTL Mapping Using Recombinant Inbred Strains'. PLoS One. 2014 Jul 17;9(7):e102307.
  • Fengxia Liu, Qingyi Liu, Guiying Wang, Weikuan Gu. 'Ramucirumab in Metastatic Renal Cell Carcinoma: The gender, racial and age issues'. Cancer. 2014 Aug 1;120(15):2379.
  • Lishi Wang, Yan Jiao, Yun Jiao, Yanhong Cao, Weikuan Gu. 'Gastric cancer drug trials—are women second class citizens?' Nat Rev Clin Oncol. 2014 Jul;11(7).
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Our Services (12)


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Affymetrix Microarray

Price on request

Our service mainly includes:

  1. extract total RNA
  • purification of the total RNA
  • analysis of the quality and quantity of total RNA (inform users if there is a problem at no addition cost)
  • DNA synthesis, cDNA purification, QC of cRNA synthesis, cRNA purification, hybridization, wash, staining, and scanning of slides
  • upload raw data of scanning to our server for users to download.

Our service mainly includes:

  1. extract total RNA
  • purification of the total RNA
  • analysis of the quality and quantity of total RNA (inform users if there is a problem at no addition cost)
  • DNA synthesis, cDNA purification, QC of cRNA synthesis, cRNA purification, hybridization, wash, staining, and scanning of slides
  • upload raw data of scanning to our server for users to download.
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Genotyping by DNA Sequencing

Starting at $40.00 per sample

Our DNA analysis service provides two kinds of services:

Mutation discovery service

We have Reveal DNA analysis system from SpectruMedix which detects novel mutations from sequences. The cost of per plate of PCR samples (less than 90 samples) is $100.

Genotyping service

We provide service for genotyping using... Show more »

Our DNA analysis service provides two kinds of services:

Mutation discovery service

We have Reveal DNA analysis system from SpectruMedix which detects novel mutations from sequences. The cost of per plate of PCR samples (less than 90 samples) is $100.

Genotyping service

We provide service for genotyping using microsatellite markers for congenic breeding. In general, the cost of genotyping per sample (less than 85 markers) is $40.

We have extensive experience in mutation detection and congenic breeding, we will be able to assist you on experimental design if you have such a project.

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Illumina RNA Microarray

Starting at $300.00 per sample

We offer the full service from tissues** to microarray data at $300.00 per sample***

Our service includes:

  1. extract total RNA
  • purification of the total RNA
  • analysis of the quality and quantity of total RNA (inform users if there is a problem at no addition cost)
  • cDNA synthesis, cDNA purification, QC of cRNA... Show more »

We offer the full service from tissues** to microarray data at $300.00 per sample***

Our service includes:

  1. extract total RNA
  • purification of the total RNA
  • analysis of the quality and quantity of total RNA (inform users if there is a problem at no addition cost)
  • cDNA synthesis, cDNA purification, QC of cRNA synthesis, cRNA purification, hybridization, wash, staining, and scanning of slides
  • upload raw data of scanning to our server for users to download.

Users need to provide enough tissues about 10mg-1000mg, depending on tissue types (or at least 1000ng of total RNA, at a minimum concentration of 30ng/ul, preferable concentration > 50ng/ul. We will purify the RNA and recheck the RNA's quality and quantity. If your RNA is degraded or otherwise unsuitable for application to an array, we will notify you immediately).

We also offer post-microarray service

We also offer the post-microarray service at a cost of $30 per sample. The post-microarray service includes:

  1. list of up/ down-regulated genes with 1, 2 or 3 fold changes upon request. The up/down-regulated genes will be listed separately decently on spreadsheets with known information about every gene
  • cluster analysis or pathway analysis of gene expression data (limited to 8) upon request.

Costs for alternative service

If users provide chips, the charge will be $145 per sample. If users provide both chips and reagents, the charge will be $120.

Notes

*Price is based on current mouse expression chips of whole genome from Illumina. It may vary based on price of types of chips and the prices. Please contact us if you want to use Affymetrix platform.

**We do not recommend users to provide RNA or to purify RNA. If users do RNA extraction, purification, and provide information on qualification and quantification, the charge will be $290. In this case, users need to provide evidence of RNA quality and quantity. More importantly, we will not be responsible for the failure because of the problem of RNA. If users provide chips, reagents, and data of RNA quality and quantity measurements, the charge will be $110 per sample.

*** Please contact us if you have a large number of samples.

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RNA Microarray Platforms

Price on request
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DNA Services

Price on request
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Biochemistry & Molecular Biology

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Biology

Price on request
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Genotyping and Gene Expression Assays

Price on request
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RNA Services

Price on request
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Nucleic Acid Services

Price on request
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DNA Microarrays

Price on request
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RNA Microarray

Price on request
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Weikuan Gu

Director

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