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Gene Targeting and Transgenic Core

Rochester, New York, US

The University of Rochester Gene Targeting and Transgenic Core's services include the production of mouse models using DNA microinjection, Cas9/CRISPR, and embryonic stem cell injection (from gene targeting in mouse ES cells to production of chimeric mice) methodologies. Additional services have included: design and construction of transgenic/knockout/CRISPR vectors and reagents, various analytical techniques, embryo cryopreservation, rederivation of pathogen-free rodents, and assisted reproduction techniques (e.g., in vitro fertilization [IVF], superovulation, and embryo transfer). Specific strains, and alternative species and methods are considered when appropriate for individual research applications.

Gene Targeting and Transgenic Core has not listed any services.

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Transgenic Animal Model Development
Starting at $6,000.00 per animal

Indel, point-mutation, knock-in and conditional knockout mouse production by CAS9/CRISPR technology
Price: $6,000 and up.

Our services include project design, gRNA and template preparation, PCR genotyping, microinjection and mouse founder breeding. We guarantee germline transmission on projects.

Indel, point-mutation, knock-in and conditional knockout mouse production by CAS9/CRISPR technology
Price: $6,000 and up.

Our services include project design, gRNA and template preparation, PCR genotyping, microinjection and mouse founder breeding. We guarantee germline transmission on projects.

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Embryonic Stem Cell Gene Targeting
Starting at $15,000.00 per animal

Production of knockout, knock-in or knock-in/conditional knockout mice.

Our services include project design, targeting vector construction, electroporation and drug screening of ES cells, Southern blot or PCR genotyping, microinjection of ES cells into blastocysts and chimera breeding. We guarantee germline transmission on projects using our ES cells.

Production of knockout, knock-in or knock-in/conditional knockout mice.

Our services include project design, targeting vector construction, electroporation and drug screening of ES cells, Southern blot or PCR genotyping, microinjection of ES cells into blastocysts and chimera breeding. We guarantee germline transmission on projects using our ES cells.

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Knockout Mouse Development
Price on request

Production of knockout, knock-in or knock-in/conditional knockout mice.

Our services include project design, targeting vector construction, electroporation and drug screening of ES cells, Southern blot or PCR genotyping, microinjection of ES cells into blastocysts and chimera breeding. We guarantee germline transmission on projects using our ES cells.

Production of knockout, knock-in or knock-in/conditional knockout mice.

Our services include project design, targeting vector construction, electroporation and drug screening of ES cells, Southern blot or PCR genotyping, microinjection of ES cells into blastocysts and chimera breeding. We guarantee germline transmission on projects using our ES cells.

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Rodent Rederivation
Price on request

The Core will use IVF and embryo transfer approach to rederive contaminated mouse lines to SPF status. After birth and weaning, all offspring will be genotyped and transferred to the investigator.

The Core will use IVF and embryo transfer approach to rederive contaminated mouse lines to SPF status. After birth and weaning, all offspring will be genotyped and transferred to the investigator.

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Mouse
Rodent in vitro Fertilization
Price on request

We provide IVF services for cryorecovery and rederivation.

We provide IVF services for cryorecovery and rederivation.

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Animal Models and Studies
Price on request
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Animal Model Housing and Maintenance
Price on request
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Biology
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Genetic Engineering
Price on request
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Transgenic Mouse Services
Price on request
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