Gateway Pharmacology Laboratories

VisualSonics Vevo 3100 Ultrasound System

Echocardiography Modes:

M-Mode

B-Mode

Pulse-Wave Doppler

Tissue Doppler

Color Doppler

Parameters:

Chamber Geometry and Thickness

Systolic and Diastolic Function

Gateway offers numerous cardiac models including:  

Myocardial Infarction (MI) - permanent and ischemia/reperfusion

Trans-Aortic Constriction (TAC) - myocardial hypertrophy

Dahl Salt Sensitive Rat (DSS) - systolic heart failure

ZSF1 Rat

Spontaneous Hypertensive Heart Failure Rat (SHHF)

ENDPOINTS:

Echocardiography: Systolic and diastolic function and geometry (M-Mode, B-Mode & Doppler)

Radio-Telemetry: Blood pressure, ECG, temperature and activity

Left Ventricular Hemodynamics: +/-dP/dt, Tau, LVEDP and LVESP

Disease Models:

5/6 Nephrectomy

Uninephrectomized

Dahl Salt Sensitive (DSS)

ZSF1 Rat

Streptozotocin (STZ)

Unilateral Urethral Obstruction (UUO)

Renal Ischemia/reperfusion (IR)

Puromycin Aminonucleoside (PAN)

Endpoints:

Proteinuria, Glomerular Filtration Rate (GFR), Creatinine Clearance (eGFR), Blood Pressure, Plasma/Urine Biomarkers, Histology/IHC 

Models:

SHR

Dahl Salt Sensitive (DSS)

ZSF1

5/6 Nephrectomy

ANGII-induced

Endpoints:

Radio-Telemetry – Continuous measurement of blood pressure, heart rate, ECG, respiration, activity and temperature in conscious, unrestrained animals for up to 6 months.

Anesthetized measurement via cannulation of carotid artery

VisualSonics Vevo 3100 Ultrasound System

Echocardiography Modes:

M-Mode

B-Mode

Pulse-Wave Doppler

Tissue Doppler

Color Doppler

Parameters:

Chamber Geometry and Thickness

Systolic and Diastolic Function

Data Sciences International (DSI) System  (120 units)

Species:

Rat, Mouse Guinea Pig

Parameters:

MAP, SBP, DBP, HR, ECG, Body Temperature and Activity

Models:

Unilateral Ureteral Obstruction (UUO)

Renal Ischemia/Reperfusion Injury (IRI)

5/6 Nephrectomy

Dahl Salt Sensitive Rat (DSS)

Endpoints:

Glomerular Filtration Rate (GFR), Proteinuria, Creatinine, BUN, Collagen/Hydroxyproline Content, Histological Evaluation

Models:

Myocardial Infarction (MI)

Myocardial Ischemia/reperfusion (IR)

Trans-Aortic Constriction (TAC)

Dahl Salt Sensitive (DSS)

Spontaneous Hypertensive Heart Failure (SHHF)

Endpoints:

Echocardiography (M-Mode, B-Mode & Doppler)

Invasive LV Hemodynamics (+/-dP/dT, Tau, LVEDP and LVESP)

Histological evaluation

Models:

Bile Duct Ligation (BDL)

Carbon Tetrachloride (CCl4)

Endpoints:

Portal Vein Pressure

Liver enzymes and Bilirubin

Collagen/Hydroxyproline Content

Histological Evaluation

Model: Monocrotaline Rat

• Right Ventricular Systolic Pressure/Pulmonary Arterial Pressure
• Heart and Lung Weight
• Myocardial, Pulmonary Artery and Lung Histology

Infusion Pharmacology Platform in unrestrained rodents

• Continuous infusion of test article for up to 14 days
• Simultaneous blood pressure & heart rate analysis
• Clinical chemistry and hematology
• Clinical observations
• Histopathology

Cardiovascular Safety

• Cardiovascular Telemetry Platform – Continuous measurement of blood pressure, heart rate, ECG, respiration, temperature & activity in conscious, unrestrained animals for up to 6 months. Blood, urine, fecal and tissue sampling available.
• Infusion Pharmacology Platform – Continuous infusion of test compound and simultaneous blood pressure & heart rate analysis for up to 14 days. Clinical observations, blood, urine, fecal and tissue sampling
• Cardiac, Lipid and Metabolic Biomarkers – Markers of tissue and biochemical damage (e.g. CK, CRP, chol, trigs, glucose, HbA1c, etc.)
• Echocardiography – Left Ventricular Function & Dimensions
• Left Ventricular Function – +/-dP/dt, Tau, LVEDP and LVESP

Renal Safety

• Renal Function – Creatinine Clearance, GFR, proteinuria and electrolytes
• Kidney Histology – Glomerulosclerosis, tubular damage, fibrosis, etc.
• Renal Biomarker Panel – Analysis of FDA/EMA approved urinary biomarkers that are predictive of drug-induced renal toxicity and translatable to human nephrotoxicity

General Safety and Toxicology

• Chronic Infusion Studies – Continuous infusion of test agent for up to 14 days. Clinical observations, blood, urine, fecal and tissue sampling
• Hematology – 23-parameter complete blood count plus 5-point WBC differential analysis on blood samples from in-life studies
• Clinical Chemistry – 180 analytes available including markers of liver, kidney, cardiac, pancreas and bone damage; as well as lipids, metabolic and inflammation
• Histopathology – Standard or tailored to your target tissue of interest

Gastrointestinal Safety and Function

• Intestinal Motility – Charcoal meal test
• Intestinal Secretion – Closed and Open loop models of fluid and electrolyte secretion as well as compound absorption

Microscopy & Histology

• We offer a wide range of microscopy and histology services to enable detailed quantitative organ/tissue analysis and morphometry
• Fixation and processing of samples into paraffin, OCT and plastic
• Sectioning and staining of tissue blocks for LM histology and EM ultrastructure
• Range of quantitative microscopy techniques including; Epi-fluorescence, Laser scanning & capture, Electron microscopy

Gateway Pharmacology Laboratories is an in vivo pharmacology laboratory that specializes in cardiovascular, renal and hepatic models and methods with unique expertise in surgical models. Contact us for more information.

Pharmacokinetic and ADME Studies

• Standard PK
• 1st Pass Metabolism
• Enterohepatic Re-circulation
• Biliary/Renal/Fecal Clearance

Cassette dosing and analysis is available and economical!

Routes of Dosing:

• Oral, Intravenous (bolus and continuous), Intraperitoneal, Subcutaneous, Intramuscular, Mini-osmotic pump

Sample Collection:

• Simultaneous blood, bile, urine and fecal sampling in conscious, unrestrained rats
• Repeated, small volume blood sampling and fluid replenishment using the Automated Blood Sampler allows for reduction in animal use and minimizes test compound quantity requirement

Species: Rat, Mouse and Guinea Pig

Gateway Pharmacology Laboratories specializes in in vivo Cardiovascular models and methods. Contact us for more information.

Models:

Bile Duct Ligation (BDL)

Carbon Tetrachloride (CCl4)

Endpoints:

Portal Vein Pressure

Liver enzymes and Bilirubin

Collagen/Hydroxyproline Content

Histological Evaluation

Pharmacology Services

Gateway Pharmacology Laboratories specializes in Cardiovascular models and methods with a core competency in surgical models. We are able to support customized models and studies within our field of expertise. Contact us for more information.

Data Sciences International (DSI) Telemetry System (120 units)

Species:

Rat, Mouse Guinea Pig

Parameters:

MAP, SBP, DBP, HR, ECG, Body Temperature and Activity

VisualSonics Vevo 3100 Ultrasound System

Echocardiography Modes:

M-Mode

B-Mode

Pulse-Wave Doppler

Tissue Doppler

Color Doppler

Parameters:

Chamber Geometry and Thickness

Systolic and Diastolic Function

Our laboratory specializes in standard and customized rodent surgical models and methods. Contact us for more information.