The Functional Genomics core facility provides infrastructure for RNAi-based Target Discovery services, from initial feasibility assessment, all the way through verification of identified targets. It also serves as a repository for reagents and assays, expertise and other technical information, and works towards technical improvements when necessary. The Core’s primary effort is dedicated to loss-of-function screening via RNAi, although gain-of-function screening for specific gene families is also available. In addition it supplies siRNAs, shRNA and ORF clones to aid the screen follow up process. Our personnel develop both plate reader and image-based cellular assays, and adapt them to HTS-siRNA/shRNA screening conditions in conjunction with the High Content Screening Core. We also collaborate with the Bioinformatics Shared Resource to aid the screening follow-up process.
Evaluate cell line transfectability through a battery of transfection reagents in parallel, both for transfection efficiency and impact on viability. For lentiviral screens, infectivity is assessed.
We generate a set of 6 lentiviral-shRNAs (either constitutive or inducible) against any given target and provide 2 ml of viral supernatant of each construct. For an additional fee we will create shRNA cell lines and determine silencing efficiency by QPCR.
Cellular assays are taken from the researcher, tested under transfection/ transduction conditions, and brought to a 384-well format. Z’ factor is assessed to determine the feasibility of the project. The assay development process is completed by performing a small screen (single 384-well plate) to determine hit frequency.
Functional Genomics (Sanford Burnham Medical Research Institute) has not received any reviews.
Functional Genomics (Sanford Burnham Medical Research Institute) has not received any endorsements.