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FRIZ Biochem GmbH

Neuried, DE

FRIZ Biochem GmbH, founded in 2004, is a privately held biotech company located in Neuried near Munich. The company, focused on advanced electronic biochips and molecular diagnostics, is knowledge driven and dedicated to provide superior solutions. Direct electrical detection of molecular recognition processes forced us to establish a proprietary anchoring of molecular probes on metal surfaces via DTPA (dithiolphosphoreamidite, US7601848, EP1626952).

This DTPA anchoring chemistry allows to provide easily customizable nanoparticle building kits with unique yet individually adjustable physico-chemical properties.

Research in nanomedicine gives insight to metabolism at molecular and nanometric level. Several areas of medical care are already benefiting from the advantages of nanotechnology.

It is our concern to support these efforts by introducing nanoSatellite kits for easy customisable biological... Show more »

FRIZ Biochem GmbH, founded in 2004, is a privately held biotech company located in Neuried near Munich. The company, focused on advanced electronic biochips and molecular diagnostics, is knowledge driven and dedicated to provide superior solutions. Direct electrical detection of molecular recognition processes forced us to establish a proprietary anchoring of molecular probes on metal surfaces via DTPA (dithiolphosphoreamidite, US7601848, EP1626952).

This DTPA anchoring chemistry allows to provide easily customizable nanoparticle building kits with unique yet individually adjustable physico-chemical properties.

Research in nanomedicine gives insight to metabolism at molecular and nanometric level. Several areas of medical care are already benefiting from the advantages of nanotechnology.

It is our concern to support these efforts by introducing nanoSatellite kits for easy customisable biological functionality for

  • tumor targeting
  • agent transport
  • bio sensoric
  • diagnostics

DTPA anchoring chemistry allows full control over thermo - and biostability as well as (mixed) loading density for individual application specifications.

Publications

  • M. Gebala, G. Hartwich, W. Schuhmann, Amplified detection of DNA hybridisation using post-labelling with a biotin-modified intercalator (2011) Faraday Disc. 149, 11 - 22.
  • M. Bandilla, A. Zimdars, S. Neugebauer, M. Motz, W. Schuhmann, G. Hartwich, A microelectrochemical sensing system for the determination of Epstein-Barr-Virus antibodies Anal. Bioanal. Chem. (2010) 398, 2617 - 2623.
  • M. Gebala, L. Stoica, D. Guschin, L. Stratmann, G. Hartwich, W. Schuhmann, A biotinylated intercalator for selective post-labeling of double-stranded DNA as a basis for high-sensitive DNA assays, Electrochem. Commun. (2010) 12, 684 - 688.
  • P. Kruppa, A. Frey, I. Kuehne, M. Schienle, N. Persike, T. Kratzmüller, G. Hartwich, D. Schmitt-Landsiedel, A digital CMOS-based 24×16 sensor array platform for fully automatic electrochemical DNA detection, Biosensors and Bioelectronics (2010) 12414 - 12419.
  • S. Neugebauer, A. Zimdars, P. Liepold, M. Gebala, W. Schuhmann, G. Hartwich, An Electrochemical DNA Assay for Salmonella spp.: Optimisation, Scanning Electrochemical Microscopy Studies and Amplification, ChemBioChem (2009) 10, 1193 - 1199.
  • P. Liepold, T. Kratzmüller, N. Persike, M. Bandilla, M. Hinz, H. Wieder, H. Hillebrandt, E. Ferrer, G. Hartwich, Electrically Detected Displacement Assay (EDDA): A Practical Approach to Nucleic Acid Testing in Clinical or Medical Diagnosis, Anal. Bioanal. Chem. (2008) Vol. 391, S. 1759 – 1772.
  • Augustyniak, M.; Paulus, C.; Brederlow, R.; Persike, N.; Hartwich, G.; Schmitt-Landsiedel, D.; Thewes, R. (2006), A 24x16 CMOS-Based Chronocoulometric DNA Microarray, Solid-State Circuits, 2006 IEEE International Conference Digest of Technical Papers, 59 - 68.
  • F. Turcu, G. Hartwich, D. Schäfer, W. Schuhmann), Ink-jet Microdispensing for the Formation of Gradients of Immobilised Enzyme Acitivity, Macromolecular Rapid Communication (2005) 26, 325 -330.
  • Flechsig, G.U., Peter, J., Hartwich, G., Wang J., Gründler, DNA Hybridisation Detection at Heated Elektrodes, Langmuir (2005) P21, 7848 - 7853.
  • M. Etienne, J. Oni, A. Schulte, G. Hartwich, W. Schuhmann, Solvent-free electrodeposition of polypyrrole as a base for the preparation of carbonised platinum microelectrodes, Electrochim. Acta (2005) 50, 5001 - 5008.
  • P. Liepold, H. Wieder, H. Hillebrandt, A. Friebel, G. Hartwich, DNA-arrays with electrical detection: A label-free low cost technology for routine use in life sciences and diagnostics, Bioelectrochemistry 6, (2005),7, 143 - 150.
  • F. Turcu, A. Schulte, G. Hartwich, W. Schuhmann, Imaging immobilised ssDNA and detecting DNA hybridisation by means of the repelling mode of scanning electrochemical microscopy (SECM) Biosensors & Bioelectronics (2004) 20, 925 - 932.
  • F. Turcu, A. Schulte, G. Hartwich, W. Schuhmann, Markerfreie elektrochemische Detektion von DNA-Hybridisierung durch Modulation des Feedbackstroms in der elektrochemischen Rastermikroskopie, Angewandte Chemie (2004) 116 3564 – 3567.
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Nanoparticle Synthesis
Price on request

FRIZ Biochem offers Gold Nanoparticles, users can easily customise to their specific needs and applications. The Au-NP Configurator is an internet based building set providing a variety of configuration options, like

  • Size
  • Anchoring chemistry
  • Density
  • No of docking oligos
    -... Show more »

FRIZ Biochem offers Gold Nanoparticles, users can easily customise to their specific needs and applications. The Au-NP Configurator is an internet based building set providing a variety of configuration options, like

  • Size
  • Anchoring chemistry
  • Density
  • No of docking oligos
  • Distal modification

Use our Au-NP Configurator to create your own customised Gold Nanoparticles!

  • ... for customised live cell RNA Detection
  • ... or easily develope your own tumor targeting and/or agent transport - with Distal Modifications
  • ... or any functionalisation mixture as well as fully customised functional nano-particle modification
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functional gold nanoparticles
nanomedicine
Dithiol Phosphoramidite (DTPA)
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DTPA / DTPA CPG are a disulfide-containing phosphoramidite / solid support to modify synthetic DNA or RNA with multiple thiol groups. These synthons have been developed to improve the attachment of oligonucleotides but also proteins or other molecules to Au, AuNO, Ag, AgNP and other thiol reactive surfaces. The dithiol can be... Show more »

DTPA / DTPA CPG are a disulfide-containing phosphoramidite / solid support to modify synthetic DNA or RNA with multiple thiol groups. These synthons have been developed to improve the attachment of oligonucleotides but also proteins or other molecules to Au, AuNO, Ag, AgNP and other thiol reactive surfaces. The dithiol can be inserted into an oligonucleotide at any position. Download DTPA-flyer

Superior stability

  • DTPA can be inserted into an oligo at the 5’ position, internally or at the 3’ position (via DTPA-CPG)
  • After reduction with TCEP or DTT each insertion results in 2 SH groups for coupling with ligands or surfaces.

Technical Information DTPA

  • C34H43N2O5PS2
  • MW 654.81
  • CAS Reg. No. 556776-24-0
  • 1,2-Diathiane-4-O-dimethoxytrityl-5-[(2-cyanoethyl)-N, N-diisopropyl)]-phosphoramidite
  • Diluent: Acetonitrile less than or equal 10 ppm water
  • Coupling: A prolonged coupling time (6 min) is recommended. To avoid oxidative cleavage of the disulfide linkage, all oxidation steps should use 0.02 M Iodine solution.
  • Deprotection: no changes needed from standard method recommended by synthesizer manufacturer for the other nucleobases used in the oligonucleotide.
  • Storage: freeze at -10 to -30 °C, dry.
  • Stability in solution: 0.1 M solution on synthesizer: approx. 24 h.

Technical Information DPTA-CPG

  • 1,2-Diathiane-4-O-dimethoxytrityl-5-succinoyl-long-chain-aminoalkyl-CPG
  • Diluent: n.a.
  • Coupling: To avoid oxidative cleavage of the disulfide linkage, all oxidation steps should use 0.02 M Iodine solution.
  • Deprotection: no changes needed from standard method recommended by synthesizer manufacturer for the other nucleobases used in the oligonucleotide.
  • Storage: freeze at -10 to -30 °C, dry.
  • Stability in solution: n.a.

TCEP Reduction Protocol

TCEP Reduction Protocol (20 - 200 nmol synthesis scale)
We recommend using Pierce’s bond-Breaker TCEP Solution (product # 77720)

  1. Add 388 µL of ultra pure water or buffer (phosphate buffer is not recommended) directly to the lyophilized oligo. Vortex until dissolved.
  2. Add 12 µL of Bond-Breaker TCEP solution to the oligonucleotide solution.
  3. For some applications, this solution can be used as is, otherwise precipitate the oligo.
  4. Add 50 µL of 3 M sodium acetate pH 5.2 and vortex.
  5. Add 1.5 mL of absolute ethanol, vortex and store at -80 °C for 20 minutes.
  6. Centrifuge at 12000 g for 10 minutes. Decant ethanol and air dry pellet.
  7. Dissolve in 200 µL of ultra pure water or buffer of choice, volume as required.
  8. Determine sample concentration by obtaining an absorbance at 260 nm.

Short cut protocols for the reduction have been established by several groups, e.g. simply adding an approx. 50-fold molar excess of TCEP to the DTPA-oligo solution for immobilization on gold.

For technical assistance please inquire. Order information

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dithiol phosphoramidite (DTPA)
Oligonucleotide Synthesis
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Highest quality multiple modified oligonucleotides are used for FRIZ Biochem's DNA -microarrays. Research & development experience in the synthesis of multiple modified probes enables FRIZ Biochem to offer customers superior reliable technologies & services to optimise their scientific endeavours.

A wide variety of... Show more »

Highest quality multiple modified oligonucleotides are used for FRIZ Biochem's DNA -microarrays. Research & development experience in the synthesis of multiple modified probes enables FRIZ Biochem to offer customers superior reliable technologies & services to optimise their scientific endeavours.

A wide variety of modifications can be incorporated into an oligonucleotide at the time of synthesis. When possible, this is done using a modified solid support (CPG) for 3'-modifications or a specialised phosphoramidite reagent for internal and 5'-modifications.

Certain modifications are not available as a modified-CPG or phosphoramidite and must be attached to the oligo after synthesis using NHS Ester chemistry. NHS Esters react with free primary amines and result in stable, covalent attachment. A primary amine is therefore added to the oligo during synthesis to permit reaction with the desired NHS Ester.

Catalog prices for modifications and prices for standard synthesis are found here.

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multiple modified oligonucleotides
Biology
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Biochemistry & Molecular Biology
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Nucleic Acid Services
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DNA Services
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DNA Synthesis and Probe Development
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Chemistry and Materials
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Materials Science
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