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FlowMetric

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Doylestown, Pennsylvania, US

About FlowMetric

Founded: 2010 Type: Privately Held Size: 11-50 employees

FlowMetric, Inc., is a leading provider of high complexity flow cytometry and cell sorting services. We serve partners in multiple fields including biotechnology, small and large cap pharmaceuticals and biotechnology companies. Established in 2010 at the Pennsylvania Biotechnology center in Doylestown, FlowMetric provides state-of-the-art GLP/GCP flow cytometry techniques that combine proprietary assays with advanced analytics to support all stages of drug development including pre-clinical, non-clinical and clinical development.

Building on over a combined 80 years of experience in flow cytometry and advanced statistical methods, our team adds actionable value to global organizations by providing solutions to your research and development needs.

Publications

  • Host Genetic Factors and Dendritic Cell Responses Associated with the Outcome of Interferon/Ribavirin Treatment in HIV-1/HCV Co-Infected Individuals Authored By: Mohit Sehgal, Marija Zeremski, Andrew H. Talal, Zafar K. Khan, Renold Capocasale, Ramila Philip, and Pooja Jain
  • Remodeling somatic nuclei via exogenous expression of protamine 1 to create spermatid-like structures for somatic nuclear transfer Authored By: Marta Czernik, Domenico Iuso, Paola Toschi, Saadi Khochbin & Pasqualino Loi
  • Discovering Molecules That Regulate Efferocytosis Using Primary Human Macrophages and High Content Imaging Authored By: Sandra Santulli-Marotto, Alexis Gervais, Jamie Fisher, Brandy Strake, Carol Anne Ogden, Chelsea Riveley, Jill Giles-Komar

Diversity Certificates

Small business

Our Services (17)


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Flow Cytometry

Price on request

Preclinical Flow Cytometry Services

Flow Cytometry is a technology that is used in all aspects of pre-clinical development ranging from target selection and validation, to mechanism of action identification. Using cells in suspension, either from cell lines or from peripheral blood, we will quantitate the response on a per-cell basis. This is especially valuable in a setting where there are multiple phenotypes in the cell suspension and the biological response is different in each subset. This is possible with cells from multiple species including, but not limited to, human, non-human primates, rodents, and canine.

  • Cell cycle analysis – Identification of cellular division stages.
  • Cell proliferation – Identification and enumeration of cellular proliferation levels.
  • Cell viability – Identification and numeration of dead (necrotic) and dying (apoptotic) cells.
  • Cell phenotyping – Identification and enumeration of different types of cells in heterogeneous mixture.
  • Cell signaling – Identification and enumeration of messaging within and between cell.
  • Intracellular Cytokine Secretion (ICS) – Identification and enumeration of cells that are secreting factors (cytokines)

Clinical Flow Cytometry Services

Flow cytometry is routinely used in clinical laboratories for the diagnosis, prognosis and monitoring of disease. This leads to familiarity within the physician and nurse practitioner community on the use and collection of blood and other fluids for flow cytometry.

At FlowMetric, we have the ability to support partners in all phases of clinical development. A few of the applications include:

Phase 0 / Translational studies:

  • Gain information and knowledge on the disease status
  • Identify patient stratification criteria
  • Identify biomarkers that are correlated with disease progression
  • Isolate / sort relevant cells for nucleic acid extraction

Phase I – Safety, Tolerability and Efficacy studies

  • Identify and quantitate activity of compound with different dosage levels (biomarker of activity)
  • Identifying PK/PD dynamics in periphery and correlate it with clinical benefit
  • Receptor Occupancy Assays

Phase II – Proof of Concept (disease)

  • Identify and confirm biomarkers to designate responders vs. non-responders

Phase III / IV

  • Generate data to support regulatory filings such as immune response to antigen post-treatment

Acute Myeloid Leukemia (AML) Chronic Myelogenous Leukemia (CML) Rheumatoid Arthritis BD LSRFortessa BD FACSAria Cell Sorting System FACS Flow cytometry Immunophenotyping Human tissue Bone marrow Blood PBMC's Biofluids Human Humanized Mouse Humanized Rat Non-human primates (NHP) Pig Rodents Immunology Oncology Hematology Show 22 more tags Show less

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Fluorescence Activated Cell Sorting (FACS)

Fluorescence Activated Cell Sorting
Price on request

Fluorescence activated cell sorting (FACS) is routinely used in pharmaceutical and biotechnology companies to isolate cells. Typically, the cells in suspension are “tagged” with fluorescent antibodies. This tag allows for pertinent cells to be identified and isolated into a liquid medium for further analysis.

With cell sorting At FlowMetric, we guide partners to achieve their biological goals, whether it is isolation of rare or abundant eukaryotic cell types. We have capabilities to isolate cells using up to 14 colors/16 parameters into 4 independent cell populations using aseptic or non-aseptic techniques.

BD FACSAria Cell Sorting System FACS

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Immunophenotyping

Price on request

At FlowMetric, we have capabilities to analyze up to 18 colors/20 parameters on a single cell simultaneously. We can interrogate intracellular and extracellular markers in the same panels. We regularly develop, validate and run pre-clinical/clinical samples for immunophenotyping in various species.

BD LSRFortessa Flow cytometry BD FACSAria Cell Sorting System FACS Immunophenotyping Blood PBMC's Human tissue Bone marrow Skin Human Humanized Mouse Humanized Rat Non-human primates (NHP) Pig Rodents Immunology Oncology Hematology Show 19 more tags Show less

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Monoclonal Antibody (mAb) Production

Price on request

FlowMetric uses proven and tested methodologies for generating your next monoclonal antibody. As a one stop source for both your hybridoma production and screening needs using your in-house/vendor generated tissue, FlowMetric can perform any, and all of the following phases in our GxP laboratories:

Monoclonal Antibody Production

  • Phase I: Fusion of Cells with the Appropriate Immortal Cell Line
  • Phase II: Primary ELISA Screen
  • Phase III: Confirmation Screens
  • Phase IV: Sub-Cloning by Limiting Dilution Assay
  • Phase V: Functional Screening & Biological Testing

The choice and flexibility are all yours! We can perform all these phases or apply only those required to fit your needs.

hybridoma fusion monoclonal

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Toxicology

Price on request

Post pre-clinical phase of drug development, the questions that arise are critical for each drug program. These questions directly relate to preparedness towards human clinical trials and enables the design of trials to allow adequate benefit-risk profiles for normal healthy volunteers as well as patients.

At FlowMetric, to answer some of the above questions for our partners, we perform flow cytometry assays that:

  • Identify changes in the number and percentage of cell types before and after treatment (Cell Phenotype).
  • Identify changes in biological (cellular) response before and after treatment by evaluating cell proliferation, cell signaling, cytokine secretion and other functional readouts.
  • Identification of change in cellular signaling before and after treatment.

Flow cytometry BD LSRFortessa BD FACSAria Cell Sorting System Toxicology GLP Bone marrow Blood PBMC's Pig Non-human primates (NHP) Rodents Human Humanized Rat Humanized Mouse Immunology Oncology Hematology Show 17 more tags Show less

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In vivo Drug Efficacy Testing

Price on request

Flow Cytometry is a technology that is used in all aspects of pre-clinical development ranging from target selection and validation, to mechanism of action identification. Using cells in suspension, either from cell lines or from peripheral blood, we will quantitate the response on a per-cell basis. This is especially valuable in a setting where there are multiple phenotypes in the cell suspension and the biological response is different in each subset. This is possible with cells from multiple species including, but not limited to, human, non-human primates, rodents, and canine.

Some examples of ways that FlowMetric routinely performs pre-clinical assays using flow cytometry are:

  • Cell cycle analysis – Identification of cellular division stages.
  • Cell proliferation – Identification and enumeration of cellular proliferation levels.
  • Cell viability – Identification and numeration of dead (necrotic) and dying (apoptotic) cells.
  • Cell phenotyping – Identification and enumeration of different types of cells in heterogeneous mixture.
  • Cell signaling – Identification and enumeration of messaging within and between cell.
  • Intracellular Cytokine Secretion (ICS) – Identification and enumeration of cells that are secreting factors (cytokines)

Flow cytometry BD FACSAria Cell Sorting System BD LSRFortessa Immunophenotyping FACS Bone marrow Blood PBMC's Human Rodents Non-human primates (NHP) Pig Immunology Oncology Hematology Show 15 more tags Show less

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Cell Cycle Analysis

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Protein Services

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Antibody Services

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Animal Models and Studies

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Biology

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Pharmacology & Toxicology

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Biomarkers

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Cells and Tissues

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Cytometry

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Bioanalysis

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Biochemistry & Molecular Biology

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Mark Maxwell

Global Director Sales & Marketing

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