The Flow Cytometry Facility, located at UMass Medical School in Worcester, provides a wide variety of cytometry services for internal and external investigators with state-of-the-art sorting and analyzing capabilities.
For analysis we currently have three 4-color FACSCaliburs, a 2 laser LSR II, a 4 laser LSRII and a 5 laser LSRII. Our cell sorters include a 3 laser Beckman Coulter MoFlo XDP hi-speed cell sorter, an all digital 3 laser 11 color FACSVantage DE DiVa sorter, a 19 parameter, 5 laser FACSAria, and a 14 parameter, 4 laser FACSAria dedicated to BSL3 cell sorting.
Two LSR II's and one Calibur are dedicated for investigator self use, after training is completed. We encourage all investigators to come to the main lab at S5-322 and learn how flow cytometry can be applied to your research.
Rates are based per hour and billed in 15 minute increments.
Training rates are based per person, per hour. Sorting includes a 1 hour setup fee unless there is a second sort on that instument that day, in which case each sort is billed 30 minutes for setup. BSL3 Sorting is billed 30 minutes for setup in all cases
These are dual-beam (air-cooled lasers) analyzers similar to the FACScan in capabilities with the addition of the second laser for four color immunofluorescence analysis. The second laser is a red-emitting diode, with output at 635nm only, suitable for excitation of APC (Allophycocyanin) or Alexa 647.
Aside from the two dedicated FACSCaliburs for core facility analysis, we also have a third instrument for investigator performed analysis (after completing our training course, or demonstrating proficiency).
The three laser MoFlo XDP digital cell sorter is configured with 2 colors on the 633nm laser, 3 on the 405nm laser which can be configured for Quantum dots as well as violet excited dyes, and 5 colors on the 488nm laser. This machine is ideal for sorting large numbers of small cells. Please note all cell sorting is a highly complex task, and is only performed by core facility personell.
The BSL3 Aria is configured with 5 parameters on the 488nm laser, 3 parameters on the 633nm laser, 3 parameters on the 405nm laser, and 3 parameters on the 350nm laser. This machine is housed in a BSL3 facility allowing us to sort unscreened human and/or infectious samples up to biosafety level 3.
The Core Lab Aria is configured with 5 parameters on the 488nm laser, 3 parameters of the 633nm laser, 3parameters on the 405nm laser, 3 parameters on the 350nm laser, and five parameters on the 561nm laser. With this machine, we are now able to sort 561nm excited fluorochromes such as mCherry. The 561 nm laser is also ideal for sorting RFP.
The LSR II is a state of the art multiparameter flow cytometer analyzer. It features the Digital DIVA hardware and software. There are 2 LSR II's housed in the LRB; LSR A "Fester" has 4 lasers and 10 fluorescent detectors and LSR B "Lurch" has 2 lasers and 8 fluorescent detectors. These LSR II's are primarily for investigator performed analysis and can be used after completing training from the core facility. The core facility LSR II has 5 lasers and 18 fluorescent detectors.
Due to the more complex nature, please schedule LSR II experiments that you would like us to run well in advance with any staff member.
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