The cell sorting and analysis lab provides access to the most sophisticated flow cytometers presently available. The core is able to analyze and physically separate cells based upon features detectable with fluorescently labeled monoclonal antibodies.
The Simches site is located in the newly constructed space located in room 3.434 in the Simches Building.
Major Equipment (Simches):
Analytic Seven Laser LSR configured with 355 nm, 407 nm, 488 nm, 532 nm, 594 nm, 633 nm, and 780 nm air cooled lasers providing a wide range of excitation possibilities.
Modified Digital Vantage cell sorter with 2 water-cooled argon, 2 krypton and one air cooled 633 nm laser.
The CNY site is located in the newly constructed space located in room 6.307 in Building 149, 13th Street, Charlestown Navy Yard.
Major Equipment (CNY):
Analytic three Laser LSR II configured with 405 nm, 488 nm, and 633 nm air cooled lasers providing a wide range of excitation possibilities.
A four laser FACS Aria II cell sorter configured with 405 nm, 488 nm, 594 nm and 633 nm air cooled lasers providing a wide range of excitation and sorting possibilities.
Our custom built BD FACSAria and FACSAriaII are high-speed cell sorters (up to 20,000 cells/second) and analyzers (up to 50,000 cells/second). They each have five lasers: 355nm UV, 407nm Violet, 488nm Blue, 532nm Green, and 633nm Red (FACSAria) or 639nm Red (FACSAriaII). Both can sort cells into plates, onto slides, or up to four different populations simultaneously into tubes.
HSCI: $50.00/hr
Other Partners: $60.00/hr
Other Academic: $125.87/hr
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