The UTHSCSA Flow Cytometry Core Facility operates at two locations; at the Long (main) campus and Greehey (north) campus in the newly-built South Texas Research Facility (STRF). The facility at the Long campus is located in room 5.044V and occupies 800 sq. ft. The STRF site is located in room 251 and occupies a total of 1306 sq. ft. The FCF is administered through the Office of the Vice President for Research in coordination with the Executive Director of the Cancer Center. Access to the flow labs is available to all UTHSCSA and external investigators with priority given to NIH-funded investigators. FCF services are based on the presence of a flow cytometry cell sorter and an analyzer at each of its two locations. The main campus site houses a Becton Dickinson Biosciences (BD) FACSAria and a BD SORP LSRII. The FACSAria cell sorter is equipped with 5-lasers enabling the analysis of up to 13 parameters and concurrent sorting of 4 different cell populations. The LSRII is equipped with 3 lasers and allows for detection of up to 8 colors at a time. The STRF site houses a MoFlow Astrios and a FACSCalibur. The MoFlow Astrios cell sorter is equipped with 4 lasers that enables analysis of up to 14 colors and concurrent sorting of 6 different cell populations. The FACSCalibur analyzer is equipped with 2-lasers and allows for the parallel detection of up to 4 colors.
The MoFlow Astrios cell sorter is equipped with 4 lasers (405nm, 488nm, 561nm, 640nm) that enable analysis of up to 14 colors and concurrent sorting of 6 different cell populations. It is one of the fastest sorters available.
The Astrios is superior in droplet accuracy and can sort into 1500 well plates, good for single cell sorting for genomic profiling. It has biosafety controls that meet the International Society for Advancement of Cytometry (ISAC) recommendation of zero aerosol escape.
$110 / hr academic / non-profit
$165 / hr commercial users
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