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Flow Cytometry Core

Belfield, Dublin, IE

Dr Alfonso Blanco directs the UCD Conway Flow Cytometry Core, which uses this incredibly efficient technique for counting and examining the physical and biological characteristics of cells and particles. The technique has many applications in humans, animals, plants and microorganisms. What really differentiates our service is the expertise of our team in assisting with the analysis of complex results for our clients and research partners.

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Biomarker Analysis
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Flow cytometry
Luminex
FACS
Fluorescence Activated Cell Sorting
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Expertise & Services:
The flow cytometry facility provides a range of services including instrumentation, workstations, training, software for analyses as well as technical expertise and advice. Trained users prepare and run their own samples. Untrained users may avail of the sample running service and results... Show more »

Expertise & Services:
The flow cytometry facility provides a range of services including instrumentation, workstations, training, software for analyses as well as technical expertise and advice. Trained users prepare and run their own samples. Untrained users may avail of the sample running service and results analyses.

Available instrumentation:
BC FC500
BC Cyan ADP "violet" (405, 488, 633nm lasers)
BC Cyan ADP "green" (488, 561, 633nm lasers)
BD Accuri C6
BD FACSAriau III cell sorter
Luminex xMAP200
MSD (MesoScale Discovery)

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Flow Cytometry
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Flow cytometry technology can be used to measure intrinsic cell characteristics such as size, shape and granularity and extrinsic cell characteristics such as DNA, internal and external receptors. This has many applications in humans, animals, plants and microorganisms with the most common applications in cell cycle,... Show more »

Flow cytometry technology can be used to measure intrinsic cell characteristics such as size, shape and granularity and extrinsic cell characteristics such as DNA, internal and external receptors. This has many applications in humans, animals, plants and microorganisms with the most common applications in cell cycle, apoptosis/necrosis, ploidy determination, immunophenotyping, protein expression and Ca2+ concentration.


Instrumentation:
- Beckman Coulter FC500
The next generation of the Beckman Coulter Epics XL-MCL is able to analyse up to 5 colours using a 488nm laser and has an automated sample loader.

  • Beckman Coulter Cyan™ADP
    Two instruments are available, each with three lasers: one with 488nm (blue), 561nm (green) and 635nm (red) and the other one with 405 nm (violet), 488nm (blue) and 635nm (red). This allows us to detect up to 11 parameters at the same time and offers complete compensation and analysis rates of up to 50,000 events/second.

  • BD Accuri C6
    Flow Cytometer® System has a high specification with 2 lasers, 2 scatter and 4 fluorescence detectors. Designed to work well with current protocols and reagents, the C6 is a high performance flow cytometer, simplified.. Extremely easy to use, it is an ideal tool for less experienced users. It is portable and can be easily moved to other laboratories.


Expertise & Services:
The flow cytometry facility provides a range of services including instrumentation, workstations, training, software for analyses as well as technical expertise and advice. Trained users prepare and run their own samples. Untrained users may avail of the sample running service and results analyses.

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Flow Cytometry Data Analysis
Price on request

Dr. Alfonso Blanco is available to assist with the design and set up of experiments, subsequent data analysis and the preparation of results for oral/ poster presentations and publication. This will facilitate the correct presentation of information and may inform the planning of future experiments.

Dr. Alfonso Blanco is available to assist with the design and set up of experiments, subsequent data analysis and the preparation of results for oral/ poster presentations and publication. This will facilitate the correct presentation of information and may inform the planning of future experiments.

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Flow Cytometry Training
Price on request

Dr. Alfonso Blanco is fully involved in several educational initiatives at national and international level.
In the UCD Flow Cytometry Core Facility.

We offer a Summer School in Flow Cytometry. Our major goal is to promote high standards in flow cytometry practice and support the development of applications. This course is... Show more »

Dr. Alfonso Blanco is fully involved in several educational initiatives at national and international level.
In the UCD Flow Cytometry Core Facility.

We offer a Summer School in Flow Cytometry. Our major goal is to promote high standards in flow cytometry practice and support the development of applications. This course is designed for scientists who wish to critically understand the principles, practice and applications of flow cytometry, appreciate the complexity of flow, be able to design flow experiments, and become competent and independent users of this technology and its tools.
Several manufacturing companies and suppliers will be involved and they will provide precise up to date information of the new instruments and reagents available.
This international course will be a 5 day program comprising of seminars and practical sessions using several instruments and remote sites. The practical portion will reinforce the concepts of the morning lectures.
We encourage participants to bring their own samples to analyse during the practical sessions. If any of the participants would like to perform the practical sessions in their mother language.
Facilities will be available in the university, including accommodation, in order to lower the cost.

The facility also provides two-day training course, which is designed to familiarise participants with the principles, practice and application of flow cytometry technology. It will comprise 2 full days of theoritical and practical sessions with different software and instrumentation.

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Luminex Multiplex Assays
Price on request

Multiplexing or multiple analyte detection offers a broad picture of the cytokines involved in a certain biological process. The measurement of cytokines and other soluble factors is becoming increasingly important in the study and management of numerous diseases.


Instrumentation:
- Luminex xMAP200
The... Show more »

Multiplexing or multiple analyte detection offers a broad picture of the cytokines involved in a certain biological process. The measurement of cytokines and other soluble factors is becoming increasingly important in the study and management of numerous diseases.


Instrumentation:
- Luminex xMAP200
The bead-based assays follow the same principle as a sandwich immunoassay. Fluorescent beads are coated with antigen-specific antibodies. A mixture of coated beads is incubated with the sample. Analytes in the sample bind to the Ab coating the beads. A biotin-conjugated Ab mix is added, which binds to the analytes bound to the capture Ab. A fluorochrome binds the biotin conjugates. One of our instruments differentiates the bead populations and calculates the analyte concentration in the samples being analysed

  • MesoScale Discovery (MSD)
    MSD's products are based on MULTI-ARRAY® technology, a proprietary combination of electrochemiluminescence detection and patterned arrays. Electrochemiluminescence detection offers a unique combination of sensitivity, dynamic range and convenience. Arrays bring speed and high density of information to discovery through miniaturization, organization and parallel processing of biological assays.

Both of the technologies provide a rapid, reliable, cost-effective and informative solution, the main benefits of multiple analyte detection are:

  • Reduced cost and labour by multiplexing
  • Requires less than 50 µl of sample
  • Shortened time-to-results by favourable reaction kinetics
  • Give faster, more reproducible results than ELISA
  • Flexible multiplexing in the range of 1 to 35 analytes meets the needs of a wide variety of applications such as protein expression profiling, RNA, autoimmune disease, genetic disease, molecular infectious disease, and HLA testing
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Expertise & Services:
The flow cytometry facility provides a range of services including instrumentation, workstations, training, software for analyses as well as technical expertise and advice. Trained users prepare and run their own samples. Untrained users may avail of the sample running service and results analyses.

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User-Run Cell Sorting
Price on request

Cell sorting allows the physical isolation of cell populations for further procedures such as cell culture and studies of protein expression.


Instrumentation:
- BD FACSAriau III Cell Sorter
This high-speed cell sorter (40,000 events/second) has 4 lasers: 488nm, 561nm, 633nm and 407nm for detection of FSC, SSC... Show more »

Cell sorting allows the physical isolation of cell populations for further procedures such as cell culture and studies of protein expression.


Instrumentation:
- BD FACSAriau III Cell Sorter
This high-speed cell sorter (40,000 events/second) has 4 lasers: 488nm, 561nm, 633nm and 407nm for detection of FSC, SSC and up to 10 fluorescent parameters. It is able to sort 4 populations of cells at the same time and perform single cell cloning in well plate platforms with a 100% purity level and 95% viability.


Expertise & Services:
The flow cytometry facility provides a range of services including instrumentation, workstations, training, software for analyses as well as technical expertise and advice. Trained users prepare and run their own samples. Untrained users may avail of the sample running service and results analyses.

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Data Services
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Computational Modeling
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Biology
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Cells and Tissues
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Cytometry
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Pharmacology & Toxicology
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Bioanalysis
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Biomarkers
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