The Bindley Bioscience Flow Cytometry and Cell Separation Facility provides advanced cell and particle analysis and sorting using flow cytometry based technology. The facility also provides training in flow cytometry analysis and sorting techniques. The facility also provides expert consultation for assay and protocol development for flow cytometry analysis and cell separation.
The FACS Aria III in our facility is housed within a biosafety cabinet making it ideal for sorting or analyzing human samples. The instrument has 5 lasers (UV, 405, 488, 561, 640) and can detect up to 14 colors. While it is primarily used for cell sorting experiments (up to four populations simultaneously) and serves as the only open access cell sorter on the Purdue campus, we will use the instrument for multi-color experiments requiring more than 5 colors and have collaborated with a number of investigators on and off campus for such studies.
The FC500 is a flow cytometry analyzer with two laser excitation wavelengths, 488nm and 633nm that can measure up to 5 colors simultaneously. This cytometer is capable of 'walk-away' operation. It runs samples in 96 well plates or 12x75 tubes. This instrument is used for immunophenotyping, phophoprotein analysis, DNA analysis, cell cycle, and functional assays.
Laser Enabled Analysis and Processing
The LEAP (Laser Enabled Analysis and Processing) is a microplate-based, interactive image cytometer with laser photoablative sorting of either attached or suspension cells.
"Jeff was very communicative with me in regards to the Validation projects he performed for us, going out of his way to help us find proper reagents and doing a great job with the work itself. I highly recommend Jeff for flow cytometry projects."
Flow Cytometry & Cell Separation Facility has not received any endorsements.