Spfng0nptgelnpegshzn logo fisabio

FISABIO Sequencing and Bioinformatics Service

ic 9.0/10 Overall Rating No Reviews Yet
ic 4 Orders Completed
Valencia, ES

About FISABIO Sequencing and Bioinformatics Service

During the last 10 years, we have observed an exponential growth of DNA sequencing methods with the development of Next Generation Sequencing (NGS). Currently in use are mostly second and third generation NGS methods, better known as high throughput sequencing or massive sequencing.
NGS opens nowadays a wide... Show more »

During the last 10 years, we have observed an exponential growth of DNA sequencing methods with the development of Next Generation Sequencing (NGS). Currently in use are mostly second and third generation NGS methods, better known as high throughput sequencing or massive sequencing.
NGS opens nowadays a wide landscape of possibilities in several fields related with biological science and public health.

Massive sequencing is allowing incredible advances in several disciplines such as:

Clinical microbiology and epidemiology
Human microbiome communities and their relationships with health and disease
Microbiological safety in Public Health in agro-food industry
Plague control
Human genetics

Sequencing facilities

The FISABIO Sequencing and Bioinformatics Service currently operates two Illumina MiSeq sequencing machines. Moreover the Service is part of the Oxford Nanopore MinIon access program, one of the last third generation sequencers.

The service also has access to molecular biology laboratories and all accessories for biological sample processing, nucleic acids extraction and sequencing libraries preparation.

The work flow goes through four separated areas to avoid cross contamination before and after amplification steps.

Informatics infrastructure

The FISABIO Sequencing and Bioinformatics Service is equipped with informatics facilities for data managing that are well adapted to the needs of massive sequencing data. Currently, the Service is equipped with a 32 Core, 128 Gb Ram server, backup units, several desktop computers and has personnel in charge of sequencing quality assessment and data delivery.

Some of last pubblications:

  • Del Campo-Moreno, R, Alarcón-Cavero, T, D'Auria, G, Delgado-Palacio, S, Ferrer-Martínez, M (2017). Microbiota and Human Health: characterization techniques and transference. Enferm Infecc Microbiol Clin. 2017 Mar 31. pii: S0213-005X(17)30101-5. http://www.ncbi.nlm.nih.gov/pubmed/28372875
  • Villar-García J, Güerri-Fernández R, Moya A, González A, Hernández JJ, Lerma E, Guelar A, Sorli L, Horcajada JP, Artacho A, D'Auria G, Knobel H. Impact of probiotic Saccharomyces boulardii on the gut microbiome composition in HIV-treated patients: A double-blind, randomised, placebo-controlled trial. PLoS One. 2017 Apr 7;12(4):e0173802. http://www.ncbi.nlm.nih.gov/pubmed/28388647
  • Moñino P., Zamorano-López N., Aguado D., Barat R., Borrás L., Seco A. and Ferrer J.. Effect of treating OFMSW and wastewater in SAnMBR technology over microbial community. 3rd IWA Specialized International Conference Ecotechnologies for Wastewater Treatment (2016) Jun 27-30; Cambridge, UK
  • Alarcón Cavero T, D'Auria G, Delgado Palacio S, Del Campo Moreno R, Ferrer Martínez M, Microbiota. 2016 59. Del Campo Moreno R (coordinadora). Procedimientos en Microbiología Clínica. Cercenado Mansilla E, Cantón Moreno R (editores). Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (SEIMC). 2016.
  • Quagliariello A, Aloisio I, Bozzi Cionci N, Luiselli D, D'Auria G, Martinez-Priego L, Pérez-Villarroya D, Langerholc T, Primec M, Micetic-Turk D, Di Gioia D. Effect of Bifidobacterium breve on the Intestinal Microbiota of Coeliac Children on a Gluten Free Diet: A Pilot Study. Nutrients. 2016 Oct 22;8(10). pii: E660. PubMedLink: http://www.ncbi.nlm.nih.gov/pubmed/27782071
  • Pérez-Lago L, Martínez-Lirola M, García S, Herranz M, Mokrousov I, Comas I, Martínez L, Bouza E, García de Viedma D. Urgent implementation in a hospital setting of a strategy to rule out secondary cases caused by imported XDR tuberculosis at diagnosis. J Clin Microbiol. 2016 Sep 28. pii: JCM.01718-16. PubMedLink: http://www.ncbi.nlm.nih.gov/pubmed/27682128.
  • Albuquerque L, Kowalewicz-Kulbat M, Drzewiecka D, Staczek P, D'Auria G, Rosselló-Móra R, da Costa MS. Halorhabdus rudnickae sp. nov., a halophilic archaeon isolated from a salt mine borehole in Poland. Syst Appl Microbiol. 2015 Dec 18. pii: S0723-2020(15)00185-X. doi: 10.1016/j.syapm.2015.12.004. PubMed PMID: 26749115. PubMedLink: http://www.ncbi.nlm.nih.gov/pubmed/26749115
  • D'Auria G, Torrents E, Luquin M, Comas I, Julián E. Draft Genome Sequence of Mycobacterium brumae ATCC 51384. Genome Announc. 2016 Apr 28;4(2). PubMedLink: http://www.ncbi.nlm.nih.gov/pubmed/27125480
Show less

Our Services (19)


ic

16S rRNA Sequencing

Starting at €58.00 per sample

16S rRNA sequencing Illumina MiSeq 2x300 (or 2x250) chemistries. PhiX control and negative controls are also included in the sequencing protocol.
After sequencing the service provide to the user:
- raw fastq data;
- cleaned fastq data after quality assessment;
- joined reads fastq files;
- a complete quality report including... Show more »

16S rRNA sequencing Illumina MiSeq 2x300 (or 2x250) chemistries. PhiX control and negative controls are also included in the sequencing protocol.
After sequencing the service provide to the user:
- raw fastq data;
- cleaned fastq data after quality assessment;
- joined reads fastq files;
- a complete quality report including all statistics before and after quality assessment.
The price is calculated on the basis of 96 samples per run. The service allows scaling up or down the number of samples.

Show less
Illumina MiSeq Amplicons Next Generation Sequencing 16S rRNA Biology Human Gut Microbiome Environmental samples Human Microbiome Microbial Isolates Show 9 more tags Show less

ic

ITS Sequencing

Internal Transcribed Spacer Sequencing
Starting at $58.00 per sample

Intergenic Transcribed Spacers (ITS) sequencing by Illumina MiSeq 2x300 (or 2x250) chemistries. PhiX control and negative controls are also included in the sequencing protocol.
After sequencing the service provide to the user:
- raw fastq data;
- cleaned fastq data after quality assessment;
- joined reads fastq files;
- a... Show more »

Intergenic Transcribed Spacers (ITS) sequencing by Illumina MiSeq 2x300 (or 2x250) chemistries. PhiX control and negative controls are also included in the sequencing protocol.
After sequencing the service provide to the user:
- raw fastq data;
- cleaned fastq data after quality assessment;
- joined reads fastq files;
- a complete quality report including all statistics before and after quality assessment.
The price is calculated on the basis of 96 samples per run. The service allows scaling up or down the number of samples.

Show less
Illumina MiSeq Eukaryotes Fungal ITS Amplicons Environmental samples Human Microbiome Show 6 more tags Show less

ic

Phylogenetic Assignation

Starting at $27.00 per sample

Taxonomy assignation of:
- 16S rRNA gene amplicons;
- Intergenic Transcribed Spacers (ITS) amplicons.
The pipeline provides the users with taxonomic assignation using user choice databases (rdp, silva, greengene). The analysis includes (see the DemoTaxonomyV4.html on the resources box):
- taxonomic assignation;
- alpha... Show more »

Taxonomy assignation of:
- 16S rRNA gene amplicons;
- Intergenic Transcribed Spacers (ITS) amplicons.
The pipeline provides the users with taxonomic assignation using user choice databases (rdp, silva, greengene). The analysis includes (see the DemoTaxonomyV4.html on the resources box):
- taxonomic assignation;
- alpha diversity analysis.
The report will include all taxonomy assignation contingency tables carried out at phylum, family and genus taxonomic ranks (species assignation depends on chosen database).

Show less
Illumina MiSeq Clostridium difficile Mycobacterium tuberculosis Prokaryotes Legionella pneumophila R package Dental Obesity Inflammatory Bowel Disease Chronic obstructive pulmonary disease (COPD) Crohn's disease 16S Bioinformatics pipelines Amplicons Shotgun DNA sequencing ITS Biology Human Gut Microbiome Environmental samples Human Microbiome Illumina Illumina NextSeq Show 22 more tags Show less

ic

16S Phylogenetic Analysis

Starting at $38.00 per sample

Taxonomy assignation and comparative analysis among multiple 16S /18S gene amplicons.
The pipeline provides the users with an extended comparative analysis
(see the DemoTaxComparativeV4.html on the resources box) which include:
- taxonomic assignation;
- alpha diversity analysis;
- beta diversity analysis;
- canonical... Show more »

Taxonomy assignation and comparative analysis among multiple 16S /18S gene amplicons.
The pipeline provides the users with an extended comparative analysis
(see the DemoTaxComparativeV4.html on the resources box) which include:
- taxonomic assignation;
- alpha diversity analysis;
- beta diversity analysis;
- canonical correspondence analysis;
- non-metric multidimensional scaling;
- mean analysis;
- etc...
The report will include all taxonomy assignation contingency tables carried out at phylum, family and genus taxonomic ranks and all figures in low and high resolution.

Show less
Illumina MiSeq Clostridium difficile Mycobacterium tuberculosis Prokaryotes Legionella pneumophila R package PCR Bioinformatics pipelines Amplicons 16S rRNA Biology Human Gut Microbiome Environmental samples Human Microbiome Show 14 more tags Show less

ic

ITS Amplicon Sequence Analysis

Internal Transcribed Spacer amplicon sequence analysis
Starting at $38.00 per sample

Taxonomy assignation and comparative analysis among multiple ITS gene amplicons.
The pipeline provides the users with an extended comparative analysis
(see the DemoTaxComparativeV4.html on the resources box) which include:
- taxonomic assignation;
- alpha diversity analysis;
- beta diversity analysis;
- canonical... Show more »

Taxonomy assignation and comparative analysis among multiple ITS gene amplicons.
The pipeline provides the users with an extended comparative analysis
(see the DemoTaxComparativeV4.html on the resources box) which include:
- taxonomic assignation;
- alpha diversity analysis;
- beta diversity analysis;
- canonical correspondence analysis;
- non-metric multidimensional scaling;
- mean analysis;
- etc...
The report will include all taxonomy assignation contingency tables carried out at phylum, family and genus taxonomic ranks and all figures in low and high resolution.

Show less

ic

Biology

Price on request
Request a quote for more information about this service.

ic

Nucleic Acid Services

Price on request
Request a quote for more information about this service.

ic

Nucleic Acid Analysis

Price on request
Request a quote for more information about this service.

ic

Biochemistry & Molecular Biology

Price on request
Request a quote for more information about this service.

ic

Sequencing Data Analysis

Price on request
Request a quote for more information about this service.

ic

Computational Modeling

Price on request
Request a quote for more information about this service.

ic

RNA Sequencing

Price on request
Request a quote for more information about this service.

ic

DNA Sequencing

Price on request
Request a quote for more information about this service.

ic

DNA Services

Price on request
Request a quote for more information about this service.

ic

RNA Services

Price on request
Request a quote for more information about this service.

ic

NGS Data Analysis

Next generation sequencing data analysis
Price on request
Request a quote for more information about this service.

ic

Phylogenetic Analysis

Price on request
Request a quote for more information about this service.

ic

Next Generation Sequencing (NGS)

Price on request
Request a quote for more information about this service.

ic

Nucleic Acid Data Analysis

Price on request
Request a quote for more information about this service.

Not finding what you're looking for?

Get info on this provider's capabilities without requesting a quote.
Missing Avatar

Giuseppe D'Auria

Sequencing and Bioinformatics Service
FileType/Other Created with Sketch.

DemoTaxComparativeV4.html

FileType/Other Created with Sketch.

DemoTaxonomyV4.html

FISABIO Sequencing and Bioinformatics Service has not received any reviews.

FISABIO Sequencing and Bioinformatics Service has not received any endorsements.