Spfng0nptgelnpegshzn logo fisabio

FISABIO Sequencing and Bioinformatics Service

Valencia, ES

During the last 10 years, we have observed an exponential growth of DNA sequencing methods with the development of Next Generation Sequencing (NGS). Currently in use are mostly second and third generation NGS methods, better known as high throughput sequencing or massive sequencing.
NGS opens nowadays a wide landscape of possibilities in several fields related with biological science and public health.

Massive sequencing is allowing incredible advances in several disciplines such as:

Clinical microbiology and epidemiology
Human microbiome communities and their relationships with health and disease
Microbiological safety in Public Health in agro-food industry
Plague control
Human genetics

Sequencing facilities

The FISABIO Sequencing and Bioinformatics Service currently operates two Illumina MiSeq sequencing machines. Moreover the Service is part of the Oxford Nanopore MinIon access... Show more »

During the last 10 years, we have observed an exponential growth of DNA sequencing methods with the development of Next Generation Sequencing (NGS). Currently in use are mostly second and third generation NGS methods, better known as high throughput sequencing or massive sequencing.
NGS opens nowadays a wide landscape of possibilities in several fields related with biological science and public health.

Massive sequencing is allowing incredible advances in several disciplines such as:

Clinical microbiology and epidemiology
Human microbiome communities and their relationships with health and disease
Microbiological safety in Public Health in agro-food industry
Plague control
Human genetics

Sequencing facilities

The FISABIO Sequencing and Bioinformatics Service currently operates two Illumina MiSeq sequencing machines. Moreover the Service is part of the Oxford Nanopore MinIon access program, one of the last third generation sequencers.

The service also has access to molecular biology laboratories and all accessories for biological sample processing, nucleic acids extraction and sequencing libraries preparation.

The work flow goes through four separated areas to avoid cross contamination before and after amplification steps.

Informatics infrastructure

The FISABIO Sequencing and Bioinformatics Service is equipped with informatics facilities for data managing that are well adapted to the needs of massive sequencing data. Currently, the Service is equipped with a 32 Core, 128 Gb Ram server, backup units, several desktop computers and has personnel in charge of sequencing quality assessment and data delivery.

Some of last pubblications:

  • Del Campo-Moreno, R, Alarcón-Cavero, T, D'Auria, G, Delgado-Palacio, S, Ferrer-Martínez, M (2017). Microbiota and Human Health: characterization techniques and transference. Enferm Infecc Microbiol Clin. 2017 Mar 31. pii: S0213-005X(17)30101-5. http://www.ncbi.nlm.nih.gov/pubmed/28372875
  • Villar-García J, Güerri-Fernández R, Moya A, González A, Hernández JJ, Lerma E, Guelar A, Sorli L, Horcajada JP, Artacho A, D'Auria G, Knobel H. Impact of probiotic Saccharomyces boulardii on the gut microbiome composition in HIV-treated patients: A double-blind, randomised, placebo-controlled trial. PLoS One. 2017 Apr 7;12(4):e0173802. http://www.ncbi.nlm.nih.gov/pubmed/28388647
  • Moñino P., Zamorano-López N., Aguado D., Barat R., Borrás L., Seco A. and Ferrer J.. Effect of treating OFMSW and wastewater in SAnMBR technology over microbial community. 3rd IWA Specialized International Conference Ecotechnologies for Wastewater Treatment (2016) Jun 27-30; Cambridge, UK
  • Alarcón Cavero T, D'Auria G, Delgado Palacio S, Del Campo Moreno R, Ferrer Martínez M, Microbiota. 2016 59. Del Campo Moreno R (coordinadora). Procedimientos en Microbiología Clínica. Cercenado Mansilla E, Cantón Moreno R (editores). Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (SEIMC). 2016.
  • Quagliariello A, Aloisio I, Bozzi Cionci N, Luiselli D, D'Auria G, Martinez-Priego L, Pérez-Villarroya D, Langerholc T, Primec M, Micetic-Turk D, Di Gioia D. Effect of Bifidobacterium breve on the Intestinal Microbiota of Coeliac Children on a Gluten Free Diet: A Pilot Study. Nutrients. 2016 Oct 22;8(10). pii: E660. PubMedLink: http://www.ncbi.nlm.nih.gov/pubmed/27782071
  • Pérez-Lago L, Martínez-Lirola M, García S, Herranz M, Mokrousov I, Comas I, Martínez L, Bouza E, García de Viedma D. Urgent implementation in a hospital setting of a strategy to rule out secondary cases caused by imported XDR tuberculosis at diagnosis. J Clin Microbiol. 2016 Sep 28. pii: JCM.01718-16. PubMedLink: http://www.ncbi.nlm.nih.gov/pubmed/27682128.
  • Albuquerque L, Kowalewicz-Kulbat M, Drzewiecka D, Staczek P, D'Auria G, Rosselló-Móra R, da Costa MS. Halorhabdus rudnickae sp. nov., a halophilic archaeon isolated from a salt mine borehole in Poland. Syst Appl Microbiol. 2015 Dec 18. pii: S0723-2020(15)00185-X. doi: 10.1016/j.syapm.2015.12.004. PubMed PMID: 26749115. PubMedLink: http://www.ncbi.nlm.nih.gov/pubmed/26749115
  • D'Auria G, Torrents E, Luquin M, Comas I, Julián E. Draft Genome Sequence of Mycobacterium brumae ATCC 51384. Genome Announc. 2016 Apr 28;4(2). PubMedLink: http://www.ncbi.nlm.nih.gov/pubmed/27125480
« Show less

FISABIO Sequencing and Bioinformatics Service has not listed any services.

Ask A Question Find what you're looking for? If not, you can ask this lab a question:
16S rRNA Sequencing
Starting at $58.00 per sample

16S rRNA sequencing Illumina MiSeq 2x300 (or 2x250) chemistries. PhiX control and negative controls are also included in the sequencing protocol.
After sequencing the service provide to the user:
- raw fastq data;
- cleaned fastq data after quality assessment;
- joined reads fastq files;
- a complete quality report including... Show more »

16S rRNA sequencing Illumina MiSeq 2x300 (or 2x250) chemistries. PhiX control and negative controls are also included in the sequencing protocol.
After sequencing the service provide to the user:
- raw fastq data;
- cleaned fastq data after quality assessment;
- joined reads fastq files;
- a complete quality report including all statistics before and after quality assessment.
The price is calculated on the basis of 96 samples per run. The service allows scaling up or down the number of samples.

« Show less
Illumina MiSeq sequencing system
Biology
16S rRNA
Amplicons
Human Gut Microbiome
Environmental samples
Human Microbiome
Microbial Isolates
Next Generation Sequencing
ITS Sequencing
Internal Transcribed Spacer Sequencing
Starting at $58.00 per sample

Intergenic Transcribed Spacers (ITS) sequencing by Illumina MiSeq 2x300 (or 2x250) chemistries. PhiX control and negative controls are also included in the sequencing protocol.
After sequencing the service provide to the user:
- raw fastq data;
- cleaned fastq data after quality assessment;
- joined reads fastq files;
- a... Show more »

Intergenic Transcribed Spacers (ITS) sequencing by Illumina MiSeq 2x300 (or 2x250) chemistries. PhiX control and negative controls are also included in the sequencing protocol.
After sequencing the service provide to the user:
- raw fastq data;
- cleaned fastq data after quality assessment;
- joined reads fastq files;
- a complete quality report including all statistics before and after quality assessment.
The price is calculated on the basis of 96 samples per run. The service allows scaling up or down the number of samples.

« Show less
Illumina MiSeq sequencing system
Eukaryotes
Amplicons
Environmental samples
Human Microbiome
Fungal ITS
Illumina MiSeq
Phylogenetic Assignation
Starting at $27.00 per sample

Taxonomy assignation of:
- 16S rRNA gene amplicons;
- Intergenic Transcribed Spacers (ITS) amplicons.
The pipeline provides the users with taxonomic assignation using user choice databases (rdp, silva, greengene). The analysis includes (see the DemoTaxonomyV4.html on the resources box):
- taxonomic assignation;
- alpha... Show more »

Taxonomy assignation of:
- 16S rRNA gene amplicons;
- Intergenic Transcribed Spacers (ITS) amplicons.
The pipeline provides the users with taxonomic assignation using user choice databases (rdp, silva, greengene). The analysis includes (see the DemoTaxonomyV4.html on the resources box):
- taxonomic assignation;
- alpha diversity analysis.
The report will include all taxonomy assignation contingency tables carried out at phylum, family and genus taxonomic ranks (species assignation depends on chosen database).

« Show less
Illumina MiSeq sequencing system
Clostridium difficile
Mycobacterium tuberculosis
Prokaryotes
R package
Dental
Biology
Amplicons
Shotgun DNA sequencing
16S
ITS
Legionella pneumophila
Human Gut Microbiome
Environmental samples
Human Microbiome
Bioinformatics pipelines
Obesity
Illumina
Inflammatory Bowel Disea
crohn disease
Illumina NextSeq
chronic obstructive pulmonary disease
16S Phylogenetic Analysis
Starting at $38.00 per sample

Taxonomy assignation and comparative analysis among multiple 16S /18S gene amplicons.
The pipeline provides the users with an extended comparative analysis
(see the DemoTaxComparativeV4.html on the resources box) which include:
- taxonomic assignation;
- alpha diversity analysis;
- beta diversity analysis;
- canonical... Show more »

Taxonomy assignation and comparative analysis among multiple 16S /18S gene amplicons.
The pipeline provides the users with an extended comparative analysis
(see the DemoTaxComparativeV4.html on the resources box) which include:
- taxonomic assignation;
- alpha diversity analysis;
- beta diversity analysis;
- canonical correspondence analysis;
- non-metric multidimensional scaling;
- mean analysis;
- etc...
The report will include all taxonomy assignation contingency tables carried out at phylum, family and genus taxonomic ranks and all figures in low and high resolution.

« Show less
Illumina MiSeq sequencing system
Clostridium difficile
Mycobacterium tuberculosis
Prokaryotes
R package
PCR
Biology
Amplicons
16S rRNA
Legionella pneumophila
Human Gut Microbiome
Environmental samples
Human Microbiome
Bioinformatics pipelines
ITS Amplicon Sequence Analysis
Internal Transcribed Spacer amplicon sequence analysis
Starting at $38.00 per sample

Taxonomy assignation and comparative analysis among multiple ITS gene amplicons.
The pipeline provides the users with an extended comparative analysis
(see the DemoTaxComparativeV4.html on the resources box) which include:
- taxonomic assignation;
- alpha diversity analysis;
- beta diversity analysis;
- canonical... Show more »

Taxonomy assignation and comparative analysis among multiple ITS gene amplicons.
The pipeline provides the users with an extended comparative analysis
(see the DemoTaxComparativeV4.html on the resources box) which include:
- taxonomic assignation;
- alpha diversity analysis;
- beta diversity analysis;
- canonical correspondence analysis;
- non-metric multidimensional scaling;
- mean analysis;
- etc...
The report will include all taxonomy assignation contingency tables carried out at phylum, family and genus taxonomic ranks and all figures in low and high resolution.

« Show less
Biology
Price on request
Request a quote for more information about this service.
Biochemistry & Molecular Biology
Price on request
Request a quote for more information about this service.
Nucleic Acid Services
Price on request
Request a quote for more information about this service.
Nucleic Acid Analysis
Price on request
Request a quote for more information about this service.
Nucleic Acid Data Analysis
Price on request
Request a quote for more information about this service.
Sequencing Data Analysis
Price on request
Request a quote for more information about this service.
DNA Services
Price on request
Request a quote for more information about this service.
DNA Sequencing
Price on request
Request a quote for more information about this service.
Next Generation Sequencing (NGS)
Price on request
Request a quote for more information about this service.
RNA Services
Price on request
Request a quote for more information about this service.
RNA Sequencing
Price on request
Request a quote for more information about this service.
Computational Modeling
Price on request
Request a quote for more information about this service.
NGS Data Analysis
Next generation sequencing data analysis
Price on request
Request a quote for more information about this service.
Phylogenetic Analysis
Price on request
Request a quote for more information about this service.
2017-11-05 23:57:24 -0800

Net Promoter Score of 10 received for 16S rRNA Sequencing.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.

Be the first to endorse FISABIO Sequencing and Bioinformatics Service

Endorse this lab