Effective Sept, 3rd 2020, Fasteris and its medical genetics partners has merged into a single company named Genesupport SA. The legal name of the new company-founded is Genesupport SA, while all NGS and Sanger services will continue under the name "Fasteris". The company and its owners remain the same.
Fasteris provides standardized and custom services for industrial and academic research laboratories, focusing on highest quality and customer satisfaction.
Our core values are Quality, Flexibility and Advice.
The company was founded in 2003 by Laurent Farinelli and Magne Osteras. In 1996, Dr Farinelli and Dr Pascal Mayer co-invented the DNA colonies, a massively parallel DNA sequencing technology that is the key of illumina NGS technology and used on HiSeq, NextSeq and MiSeq. In 2007, Fasteris was the first service company in the world to acquire an illumina NGS instrument, the Solexa Genome Analyzer.
Fasteris is one of the world's leading biotechnology company, continuously developing a wide range of applications and services in the areas of next-generation (NGS) and Sanger sequencing.
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Sample preparation, sequencing method and personalized advice are the keys to successful NGS projects. Fasteris SA is offering you the best expertise in each of these areas.
Sample preparation : We tailor options for each protocol to perfectly fit your project needs and to answer your biological questions.
DNA sequencing methods : Depending on your project aim and need, the necessary number of reads and also your time constraints, we choose together the best sequencing platform and run mode for your NGS project.
Transcriptome sequencing (RNA-seq) is a Next-Generation Sequencing method that enables to explore the complete set of transcripts of living cells of model and non-model organisms. Such investigation is essential for interpreting the unique functional organism status in normal physiological conditions or diseases, in response to various stimuli or at different developmental stages.
Transcriptome sequencing generates millions of strand-orientated reads derived from mRNA or/and non-coding RNA populations converted into cDNA. The method allows not only precise and accurate quantification of gene expression, but also identification of novel transcripts and isoforms (i.e. alternatively-spliced transcripts).
Fasteris offers a wide range of protocols to help finding the answer to your biological questions:
Please note: None of the above three protocols is adapted or suitable for transcripts smaller than 100 bp. Thus, if you are looking for miRNA expression, please consider the small RNA protocol. In addition, it is essential to perform DNase treatment prior to library preparation. It is strongly recommended to perform it during the RNA extraction procedure.
NGS sequencing options:
Fasteris offers sequencing on Illumina HiSeq 2500, HiSeq 4000 and MiSeq platforms, with up to 96 libraries multiplexed per lane. Coverage can be adjusted to your needs.
De novo sequencing is a Whole-Genome Sequencing (WGS) method that allows sequence determination of a novel genome with high resolution and accuracy. It is suitable for analysis of genomes of any size and complexity.
De novo sequencing approach differs from the genome re-sequencing method by the library construction setup. In addition to shotgun libraries it is highly recommended making use of libraries derived from larger genomic fragments. This approach will significantly improve the quality of the assembly and the average scaffold size of the derived contigs.
For organisms without reference (de novo) Fasteris offers a combination of following library preparation protocols:
NB: For de novo sequencing approach we recommend aiming at least 50-fold to 70-fold genome coverage.
NGS sequencing options:
Fasteris offers sequencing on Illumina HiSeq 2500, HiSeq 4000 and MiSeq platforms, coverage can be adjusted to your needs.
Small RNA sequencing (small RNA-seq) is a Next-Generation Sequencing method allowing to explore small non-coding RNAs (18-35 nt long). Small RNA molecules are the powerful mediators, regulating gene expression at the transcriptional, post-transcriptional and translational levels in organisms of different complexity. Small RNAs play an important role in organism functional regulation in normal physiological conditions (cell proliferation, apoptosis) or diseases, such as cancer. Small RNA-seq also allows analysing virus and infected host (such as plants) interactions and even reconstructing viral genome.
The three major well-known and well-described small RNA classes are: microRNAs (miRNAs), interfering RNAs (siRNAs) and Piwi-associated RNAs (piRNAs). Small RNA-seq allows to examine the differential expression of all these 3 small RNA classes and also to discover novel small RNAs.
For small RNA studies Fasteris offers the following protocols:
NGS sequencing options:
Fasteris offers sequencing on Illumina HiSeq 2500, HiSeq 4000 and MiSeq platforms, coverage can be adjusted to your needs.
Chromatin immunoprecipitation (ChIP) is a method used for detection of interactions between protein and DNA in living cells. Next-Generation Sequencing of the derived and purified DNA allows the identification of genomic regions associated with specific proteins even if the sequence of the organism is unknown. The approach is used for identification of DNA binding sites of transcription factors, histone localization, etc.
ChIP-seq library preparation is performed with illumina TruSeq ChIP kit and up to 24 samples can be multiplexed in the sequencing lane.
NGS sequencing options:
Fasteris offers sequencing on Illumina HiSeq 2500, HiSeq 4000 and MiSeq platforms, coverage can be adjusted to your needs.
Transposon sequencing (Tn-seq) is a Next-Generation Sequencing (NGS) method for genome-wide scale profiling allowing accurate detection of transposon insertions sites.
Most commonly Tn-seq is used for the analysis of microorganism mutant pools to identify genetic aspects associated with certain phenotypes or to get insight to genetic basis required for fitness; for example such as antibiotic resistance gene identification by comparison of the mutant abundance after the growth in the selective conditions.
Fasteris in house developed Tn-seq protocol can be adjusted to your experimental needs, depending on the transposon used.
We use the highest quality Sanger (Capillary) DNA Sequencing methods, personalized advice and next day results.
From Economy to Full service or from Ready-to-Run to Customized service, we offer the most flexible solutions to suit your needs.
We are dedicated to providing the highest quality sequences from any sample.
We have one of the longest and most extensive experience as service provider with the Illumina platform.
We provide unparalleled customer support, not only with experimental design, library preparation options and sequencing methods, but also with bioinformatics analyses.
Our combined expertise is particularly useful for difficult projects that require innovative solutions, but also quite valuable for new customers.
We strive to provide biologically relevant information by using a variety of tools to visualize and interpret the results.
We are always ready to discuss innovative ideas and help you achieve the optimal results.
"Fasteris SA was the first company to respond. They called me on the phone to discuss the project. We agreed for them to analyze existing third-party data, based on which Fasteris SA was supposed to provide a quote for the full genome sequencing. Their analysis didn't add any new information to the project. They suggested not to proceed with the full genome sequencing and never provided the quote for the service. Wasted time and money."
"Extremely professional and very helpful"
"Excellent service on time!"
"Excellent service, on time, very helpful technical support."
Genesupport SA ("Fasteris") has not received any endorsements.