Headquartered in Davis, California, Expression Systems is dedicated to supplying and servicing the cell culture and bio-industrial markets with innovative media formulations, complementary products and contract services. We specialize in baculovirus/host cell systems with an emphasis on cell culture of insect and mammalian cell lines.
Our unique focus on BEVS (Baculovirus Expression Vector System) applications helps researchers and clinicians in academic, biotech and pharmaceutical fields around the world work at the highest level of consistency. Our products and services are used by elite institutions, companies and labs around the world. At any step in the process, from gene synthesis to protein purification, Expression Systems helps achieve best-in-class results.
Construction of a Bacmid using a pre-made pFastBac vector. Includes PCR confirmation of Bacmid clone containing GOI
Expression Systems is pleased to offer baculovirus titering services to help our customers maximize expression and reproducibility. Baculovirus titering is routinely set up on Wednesdays with data delivery Thursday afternoon PST. Samples should be received by 11AM Wednesday to be set up that week.
Accurate titering of recombinant baculovirus is an important step in protein production optimization. A high throughput, flow cytometric assay is utilized to quantitate infectious baculovirus particles produced in insect cell culture, allowing for accurate virus titer determination for multiple conditions in less than 24 hours. The assay detects the cell surface expression of gp64 after a short incubation period allowing for a single round of infection. Virus infected cells are then assessed by flow cytometric analysis for gp64 expression.
Ship 0.5-1 ml of virus stock (as little as 200 µl can be titered with advance notice)
Ship sterile aliquots in 2 ml screw cap tubes or equivalent. Ship overnight on cold packs.
Please use the form on this page under the “Titering Service Form” tab or at checkout.
Expression Systems will optimize the production parameters for your product. Recombinant protein, virus-like particle or ene therapy vector expression will be optimized in one or more cell lines by doing a time-course study of infection, varying infection density, multiplicity of infection (MOI), and harvest time. Scale up conditions and process development are also offered by Expression Systems.
Expression Systems has dedicated clean rooms for your project. Inquire about GMP/GLP manufacturing. Expression Systems will produce your protein of interest using conditions specified by you or determined by the expression optimization.
Protein production services include:
Expression Systems can quickly produce baculovirus stock from your gene of interest. The final product can be a minimum of 500 ml up to hundreds of liters at a titer of at least 5 x 108 infectious units per ml. Titer is determined using the flow cytometric based assay that is also offered as a service. The stock can be generated from a pre-existing virus stock or by cotransfection using a transfer vector containing the gene of interest provided by you. Baculovirus stocks are generated using Sf9 cells in your choice of ESF 921 or ESF AF media. If desired, baculovirus stocks can be purified by end point dilution or plaque purification.
Baculovirus stock production services include:
Expression Systems can clone your gene of interest into any commercial baculovirus expression vector or our own vectors. Starting from your cDNA clone or an accession number we will guide expression cassette design and clone your gene into a transfer vector. We can add tags at the N or C terminus, add secretion signals, and do site-directed mutagenesis. We will sequence validate the expression vector for you and produce recombinant baculovirus from your clone.
Molecular biology services include:
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