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Expression Systems

Davis, California, US

Headquartered in Davis, California, Expression Systems is dedicated to supplying and servicing the cell culture and bio-industrial markets with innovative media formulations, complementary products and contract services. We specialize in baculovirus/host cell systems with an emphasis on cell culture of insect and mammalian cell lines.

Our unique focus on BEVS (Baculovirus Expression Vector System) applications helps researchers and clinicians in academic, biotech and pharmaceutical fields around the world work at the highest level of consistency. Our products and services are used by elite institutions, companies and labs around the world. At any step in the process, from gene synthesis to protein purification, Expression Systems helps achieve best-in-class results.

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Bacmid Preparation
Starting at $2,000.00 per prep

Construction of a Bacmid using a pre-made pFastBac vector. Includes PCR confirmation of Bacmid clone containing GOI

Construction of a Bacmid using a pre-made pFastBac vector. Includes PCR confirmation of Bacmid clone containing GOI

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Large Scale Plasmid Prep
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Plasmids and Vectors
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Cloning Vectors
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Gene Synthesis
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Baculovirus Titering Service
Starting at $165.00 per sample

Expression Systems is pleased to offer baculovirus titering services to help our customers maximize expression and reproducibility. Baculovirus titering is routinely set up on Wednesdays with data delivery Thursday afternoon PST. Samples should be received by 11AM Wednesday to be set up that week.

Description

Accurate... Show more »

Expression Systems is pleased to offer baculovirus titering services to help our customers maximize expression and reproducibility. Baculovirus titering is routinely set up on Wednesdays with data delivery Thursday afternoon PST. Samples should be received by 11AM Wednesday to be set up that week.

Description

Accurate titering of recombinant baculovirus is an important step in protein production optimization. A high throughput, flow cytometric assay is utilized to quantitate infectious baculovirus particles produced in insect cell culture, allowing for accurate virus titer determination for multiple conditions in less than 24 hours. The assay detects the cell surface expression of gp64 after a short incubation period allowing for a single round of infection. Virus infected cells are then assessed by flow cytometric analysis for gp64 expression.

Ship 0.5-1 ml of virus stock (as little as 200 µl can be titered with advance notice)

Ship sterile aliquots in 2 ml screw cap tubes or equivalent. Ship overnight on cold packs.

Please use the form on this page under the “Titering Service Form” tab or at checkout.

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Flow Cytometry
Flow cytometry
Sf9
Baculovirus Protein Expression
Price on request

Expression Systems will optimize the production parameters for your product. Recombinant protein, virus-like particle or ene therapy vector expression will be optimized in one or more cell lines by doing a time-course study of infection, varying infection density, multiplicity of infection (MOI), and harvest time. Scale up... Show more »

Expression Systems will optimize the production parameters for your product. Recombinant protein, virus-like particle or ene therapy vector expression will be optimized in one or more cell lines by doing a time-course study of infection, varying infection density, multiplicity of infection (MOI), and harvest time. Scale up conditions and process development are also offered by Expression Systems.

Expression Systems has dedicated clean rooms for your project. Inquire about GMP/GLP manufacturing. Expression Systems will produce your protein of interest using conditions specified by you or determined by the expression optimization.

Protein production services include:

  • Optimization of expression and scale up parameters
  • Production of protein of interest
  • Bench scale to production scale
  • Subunit vaccine, Virus Like Particle or Gene therapy (rAAV) active pharmaceutical ingredient
  • Protein purification
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BIIC (Baculovirus) Stock Generation Service
Price on request

Expression Systems can quickly produce baculovirus stock from your gene of interest. The final product can be a minimum of 500 ml up to hundreds of liters at a titer of at least 5 x 108 infectious units per ml. Titer is determined using the flow cytometric based assay that is also offered as a service. The stock can be generated... Show more »

Expression Systems can quickly produce baculovirus stock from your gene of interest. The final product can be a minimum of 500 ml up to hundreds of liters at a titer of at least 5 x 108 infectious units per ml. Titer is determined using the flow cytometric based assay that is also offered as a service. The stock can be generated from a pre-existing virus stock or by cotransfection using a transfer vector containing the gene of interest provided by you. Baculovirus stocks are generated using Sf9 cells in your choice of ESF 921 or ESF AF media. If desired, baculovirus stocks can be purified by end point dilution or plaque purification.

Baculovirus stock production services include:

  • Production of high titer (> 5 x 10e8) baculovirus stock
  • Virus stock scale up to 100s of liters
  • Baculovirus stock purification by end point dilution or plaque purification
  • Titering services
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Biochemistry & Molecular Biology
Price on request

Expression Systems can clone your gene of interest into any commercial baculovirus expression vector or our own vectors. Starting from your cDNA clone or an accession number we will guide expression cassette design and clone your gene into a transfer vector. We can add tags at the N or C terminus, add secretion signals, and do... Show more »

Expression Systems can clone your gene of interest into any commercial baculovirus expression vector or our own vectors. Starting from your cDNA clone or an accession number we will guide expression cassette design and clone your gene into a transfer vector. We can add tags at the N or C terminus, add secretion signals, and do site-directed mutagenesis. We will sequence validate the expression vector for you and produce recombinant baculovirus from your clone.

Molecular biology services include:

  • Gene sequence codon optimization
  • Gene synthesis
  • Vector design and cloning
  • Plasmid scale up and purification
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Custom Gene Cloning
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Biology
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Protein Services
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Protein Expression
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Nucleic Acid Services
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DNA Services
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DNA Synthesis and Probe Development
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Molecular Cloning
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Recombinant Protein Expression
Price on request
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Protein Expression Optimization
Price on request
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