Expedeon Ltd

Antibody micro-optimization service

Optimizing your antibody conjugates can be time consuming and difficult and can require significant amounts of antibody and label. Expedeon antibody micro-optimization service is a fast and efficient way to optimize your antibody conjugates using very small quantities of antibody.

Our Lightning-Link® conjugation kits enable scientists to generate high quality conjugates with only 30 seconds hands-on time. However, for those scientists that require that little bit extra performance, be it greater sensitivity or lower background, our micro-optimization service may well be the answer.

The conjugation service is performed by our experienced conjugation scientists here at Expedeon. They use a combination of proprietary and traditional conjugation techniques to generate a range of different conjugates for you to test in your chosen application.

Benefits of this service include:

• A choice of 3 different scales of conjugate 50ug, 100ug and 500ug
• Over 30 different labels available including enzymes, fluorescent dyes, fluorescent proteins and tandem labels
• Antibody clean-up prior to conjugation
• Level of conjugation controlled by a variety of parameters unique to Expedeon
• Evidence of successful conjugation provided
• Scalable
• Fast turnaround time
• Reproducible
• Full technical support

What’s included?

• 6 different conjugates at the chosen scale
• Antibody clean-up prior to conjugation
• Proof of successful conjugation
• Fast turnaround time
• Full technical support

Furthermore if you need to perform multiple conjugations there is the option to convert your favoured formulation to a bespoke Lightning-Link DIY kit!

We can make your life even easier by supplying and purifying your antibody if required.

Our standard Lightning-Link kits have been designed to work with all IgGs irrespective of species or subtype and will generate excellent conjugates. However sometimes our customers find time restraints can mean outsourcing their conjugation is more effective. Our antibody conjugation service is performed in-house by our expert conjugation scientists, providing you with your required antibody conjugate at your agreed conjugation ratio and scale.

Benefits of this service include:

• Performed by conjugation professionals
• Agreed conjugation ratio and scale
• Evidence of successful conjugation provided
• Reproducible
• Fast turnaround time
• Full technical support
• Applicable to other proteins

If you have a need to perform multiple conjugations we can also provide you with a bespoke DIY kit!

What’s included?

• Generation of conjugate at required scale from 1mg to 1g
• Antibody clean up prior to conjugation (removal of BSA or azide)
• Antibody buffer exchanged in to a ‘conjugation friendly’ buffer if required
• Evidence of successful conjugation provided
• Fast turnaround time
• Full technical support

We can make your life even easier by supplying and purifying your antibody if required.

Antibody-oligo Conjugation

Generating antibody/oligonucleotide conjugates can be time-consuming and difficult. Our antibody/oligonucleotide conjugation service can provide you with the antibody/oligonucleotide conjugates you require.

Benefits of this service include:

• Performed by trained professionals
• Agreed conjugation ratio and scale
• Evidence of successful conjugation provided
• Reproducible
• Fast turnaround time
• Full technical support
• Applicable to other proteins

If you have a need to perform multiple conjugations we can also provide you with a DIY kit!

What’s included?

• Generation of conjugate at required scale from 1mg to 1g
• Conjugate at required antibody:oligo ratio
• Antibody clean up prior to conjugation (removal of BSA or azide)
• Antibody buffer exchanged in to a ‘conjugation friendly’ buffer if required
• Unbound oligo removal post conjugation
• 1D gel showing successful conjugation
• Fast turnaround time

We can make your life even easier by supplying the oligo, sourcing your antibody and purifying your antibody if required.

Lateral Flow Assay Development Services

Expedeon’ full lateral flow assay (LFA) development service will take your assay development from initial idea, through to R&D and then transfer to trusted partners for bulk manufacturing, due to the recent partnership with Abingdon Health Ltd (“Abingdon”) which will enable Expedeon to provide its customers with full lateral flow assay design and supply services .

Although this service is a natural follow on from our InnovaCoat® GOLD Optimization service, the service is divided into four modules so customers have the flexibility to pick and choose only the services they require.

The full service (all four modules) is a multi-step project providing you with different options, from proof of principle LFA using a dipstick (with either spots or lines on the nitrocellulose membrane), to full strips with lines (or spots), sample pad, conjugate pad and absorbent pad.

Other services available include our LFA optimization service and strip manufacturing service for LFA, please see below for more details.

Custom gold nanoparticle conjugation service

Our custom conjugate formulation service can either be used as a follow-up service from our micro-optimization service, providing you with bulk quantities of your bespoke antibody-gold nanoparticle conjugates or as an independent conjugation service providing you with specialized conjugates of your choice.

Our in-house team of expert conjugation scientists will quickly generate your conjugates at any scale in your chosen buffer and at an optical density of your choice.

This service simply requires that you provide us with your purified antibody and selected conjugate formulation.

Alternatively, we can provide a full purification service prior to conjugation or purchase commercially available antibodies on your behalf.

Benefits of this service:

• Your preferred conjugate formulation at any scale
• Your preferred concentration and buffer
• Batch-to-batch reproducibility
• Fast turnaround
• No loss of materials

What’s included?

• Liquid InnovaCoat® GOLD conjugates at any scale
• Clean-up of purified antibody prior to conjugation (if required)
• Removal of unbound antibody post conjugation
• Proof of successful conjugation

Additional extras

• The antibody can be purified or provided if required

TrueAdvance Whole Genome Amplification Services

Based on a technology with higher sensitivity, suitable for very reduced DNA samples

High quality samples with high genome coverage due to reduced random primers bias

Additional QC steps to ensure quality before any downstream application

TrueAdvance is Expedeon’s services for Whole Genome Amplification (WGA), for single-cells, circulating cancer DNA (cell-free DNA) or purified DNA. We offer a unique set of expertise, innovative technologies and competitive pricing to open the way to single-cell genomics, circulating tumor DNA (cell-free DNA) analysis, and any other low-level DNA studies.

Benefits of this service include:

• It is based on a technology with higher sensitivity that can amplify even very low input DNA samples (down to femtograms)
• The amplified DNA is of higher quality in terms of genome coverage because TruePrime technology avoids random primers bias
• The service includes additional QC steps that ensure the quality of the final sample: DNA purification, DNA quantification and a PCR-based genome coverage check

What’s included?

We take care of whole workflow after sample collection to single-cell or cell-free DNA genomics

Electrophoresis Equipment and Reagents, Western Blotting Equipment and Reagents, Bioconjugation Kits, Genomics Products, Mass Spectrometry preparation products, Protein Purification Resins, and later flow assay kits.

Electrophoresis Equipment and Reagents, Western Blotting Equipment and Reagents, Bioconjugation Kits, Genomics Products, Mass Spectrometry preparation products, Protein Purification Resins, and later flow assay kits.

Antibody Services

Protein Services

Materials Science Services

Chemistry and Materials Services

DNA Amplification Services

DNA Services

Nucleic Acid Services

Biochemistry & Molecular Biology Services

Cell Culture Media and Reagents Services

Cells and Tissues Services

Biology Services

Laboratory Equipment and Facility Management Services