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ExonBio

San Diego, California, US

With a longstanding tradition of quality and service, ExonBIO expands the capabilities of academic laboratories, biotechnology companies and pharmaceutical companies involved in generating new leads and developing therapeutics. We work in partnership with our clients to ensure the most efficient and cost effective way to generate superior recombinant proteins for structural based drug discovery, HTS, and functional validation for drug discovery.

Our novel proprietary Single Plasma Cell Interrogation (SPIN®) technology capable of developing monoclonal antibodies from any animals (rabbit, Llama, chicken, cow, sheep or human), the broad epitope coverage and high affinity made the technology perfect for therapeutics and diagnostic antibody development. Our mammalian transient expression platforms are revolutionary in recombinant protein and antibody production. Our know-how and experience enable us to provide premium... Show more »

With a longstanding tradition of quality and service, ExonBIO expands the capabilities of academic laboratories, biotechnology companies and pharmaceutical companies involved in generating new leads and developing therapeutics. We work in partnership with our clients to ensure the most efficient and cost effective way to generate superior recombinant proteins for structural based drug discovery, HTS, and functional validation for drug discovery.

Our novel proprietary Single Plasma Cell Interrogation (SPIN®) technology capable of developing monoclonal antibodies from any animals (rabbit, Llama, chicken, cow, sheep or human), the broad epitope coverage and high affinity made the technology perfect for therapeutics and diagnostic antibody development. Our mammalian transient expression platforms are revolutionary in recombinant protein and antibody production. Our know-how and experience enable us to provide premium quality molecular biology and protein expression services to academia, biotech and pharmaceutical companies.

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ExonBio has not listed any services.

Hybridoma Sequencing
Price on request

ExonBIO offers fast and professional antibody cloning and sequencing services for your valuable monoclonal antibodies. We can sequence from any antibody producing cell line with a service that can be tailored to your requirements.

Why sequence your McAb:

  • Patent Application. If you intend to patent your MAb, antibody... Show more »

ExonBIO offers fast and professional antibody cloning and sequencing services for your valuable monoclonal antibodies. We can sequence from any antibody producing cell line with a service that can be tailored to your requirements.

Why sequence your McAb:

  • Patent Application. If you intend to patent your MAb, antibody sequence will be necessary in order to distingush your novel antibody from others.
  • Antibody Recombination. To determine the sequence of the MAb, the cDNA of your antibody can be isolated from the hybridoma cell line and its subcloning and expression in mammalian cells.
  • Antibody Humanization. Antibody sequence data is vital for any downstream antibody engineering/humanization.

What we offer:

  • V-region cloning and sequencing. V-regions of heavy and light chains of antibody were amplified by degenerated primers and cloned into a pSUPER-blunt TOPO cloning vector. At least 10 clones each from heavy and light chains were sequenced.
  • Full-length antibody cloning service.Clone and seqeuncing the full-length IgG gene by 5'RACE to amplify, clone and sequencing the 5' complete sequence of hevy and light chains. After obtained the 5'end sequences, amplify the full-length heavy and light chains and clone into a cloning vector or expression vector for downstream work.
  • No Clone Left Behind. It is often observed that more than one clone is found in a monoclonal hybridoma cell line. We will make sure that all the IgG clones are sequenced from your hybridoma with the highest quality.
  • 100% Success Rate. Some of the hybrdomas are more difficulty to be cloned due to the abundant endogenous aberrant kappa chain. We take multiple approaches and extra efforts to find the right clone. We never deliver a single heavy chain to our customers.
  • Fast Turnaround. Complete amplification, cloning, sequencing and analysis of antibody in only 2 weeks. Fastest service in the field.
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Antibody Engineering Services
Price on request

Antibody engineering holds the potential for creating antibodies with multiple specificities, greater affinity for their targets, and fewer side effects. Among these approaches, more-or-less traditional protein engineering remains a topic of intense interest.

  • V-region Cloning and Sequencing: Clone V-region from hybridomas or... Show more »

Antibody engineering holds the potential for creating antibodies with multiple specificities, greater affinity for their targets, and fewer side effects. Among these approaches, more-or-less traditional protein engineering remains a topic of intense interest.

  • V-region Cloning and Sequencing: Clone V-region from hybridomas or B-lymphocytes with 5’RACE technology.
  • Full-length Antibody Cloning: Clone full-length HC and LC from hybridomas into a mammalian expression vector.
  • Isotype Switching: Switch isotypes of antibodies to increase the production or activities.
  • Antibody Affinity Maturation: Enhance the antibody affinity by phage display technology - PhD Antibodies
  • Antibody Humanization: Humanization of antibodies from mouse hybridomas and maintains the affinity - PhD Antibodies
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Stable Cell Line Generation
Price on request

Stable Cell Pool: With stable promoter and antibiotic selection, the stale cell pool can produce grams of antibody within short timeline.
Stable Cell Line: Either for research or for manufacture, we can generate stable cell line at the yield of 1 gram/liter industrial production level.

Stable Cell Pool: With stable promoter and antibiotic selection, the stale cell pool can produce grams of antibody within short timeline.
Stable Cell Line: Either for research or for manufacture, we can generate stable cell line at the yield of 1 gram/liter industrial production level.

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Monoclonal Antibody (mAb) Production
Price on request

Developing monoclonal antibody from common species, such as rabbit, llama or chicken; or not so common species, such as shark, cow, sheep or vaccinated/pathogen infected human.
SPIN® technology started with plasma cells, the most mature B cell population and screening antigen-specific antibody clones one by one avoid the bias of... Show more »

Developing monoclonal antibody from common species, such as rabbit, llama or chicken; or not so common species, such as shark, cow, sheep or vaccinated/pathogen infected human.
SPIN® technology started with plasma cells, the most mature B cell population and screening antigen-specific antibody clones one by one avoid the bias of epitope selection. Antibody generate by SPIN® shows high affinity and broad diversity.

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Rabbit
Chicken
Camelids
Antibody Discovery
Price on request

Our proprietary Single Plasma Cell Interrogation (SPIN® ) platform uses microfluidic technology to combine the identification and isolation of antigen-specific matured plasma cells. Automation-facilitated high-throughput screening has made the interrogation of the natural antibody repertoire possible.

Our proprietary Single Plasma Cell Interrogation (SPIN® ) platform uses microfluidic technology to combine the identification and isolation of antigen-specific matured plasma cells. Automation-facilitated high-throughput screening has made the interrogation of the natural antibody repertoire possible.

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Peptide Synthesis
Price on request

As part of our additional services, exonbio provides SPIN® Grade peptide synthesis services.

SPIN® Grade: purity > 95%, LC-MS verified.

Applications: Immunization, Antigen specific cell selection and Antibody screening

As part of our additional services, exonbio provides SPIN® Grade peptide synthesis services.

SPIN® Grade: purity > 95%, LC-MS verified.

Applications: Immunization, Antigen specific cell selection and Antibody screening

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Bacterial Protein Expression
Price on request
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Protein Expression in Mammalian Cells
Mammalian cell based recombinant protein production
Price on request

Mammalian transient expression with proprietary transfection reagent (TransPERfect®), 5-fold cheaper than the MAX reagent from Invitrogen, 2-fold higher yield than MAX reagent.
High yield and efficient purification (protein A, Nickel, Ion exchange, Heparin, SEC), QC with SDS-PAGE, analytic SEC, Endotoxin-free.

Mammalian transient expression with proprietary transfection reagent (TransPERfect®), 5-fold cheaper than the MAX reagent from Invitrogen, 2-fold higher yield than MAX reagent.
High yield and efficient purification (protein A, Nickel, Ion exchange, Heparin, SEC), QC with SDS-PAGE, analytic SEC, Endotoxin-free.

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Chemistry and Materials
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Mammalian Cell Culture
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Therapeutic Monoclonal Antibody (mAb) Development
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DNA Sequencing
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Synthetic Chemistry
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Biochemistry & Molecular Biology
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Protein Production
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Biology
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Nucleic Acid Services
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Cells and Tissues
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Antibody Services
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DNA Services
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Protein Services
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