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Eurofins DiscoverX Corporation

Fremont, California, US

To fully assist drug discovery and development, DiscoverX® provides unique and proprietary target- and phenotypic assay solutions that span the entire drug discovery continuum, including:

  • The largest menu of thoroughly validated human assays against potential drug targets, removing lengthy in-house assay development efforts for screening and selectivity testing.
  • Over 15 years of experience working with large and small drug discovery groups in pharma and biotech, consistently ranked as offering the highest quality services.
  • Fast delivery of comprehensive data reports ensuring critical decisions can be made with confidence and speed.

Highlights of DiscoverX Service Portfolio:

  • KINOMEscan® and PathHunter® Screening and Profiling Services are complete solutions for screening and profiling compounds using GPCR, Kinase, Epigenetic, NHR, Pathway, Interleukin, and Checkpoint targets to identify quality... Show more »

To fully assist drug discovery and development, DiscoverX® provides unique and proprietary target- and phenotypic assay solutions that span the entire drug discovery continuum, including:

  • The largest menu of thoroughly validated human assays against potential drug targets, removing lengthy in-house assay development efforts for screening and selectivity testing.
  • Over 15 years of experience working with large and small drug discovery groups in pharma and biotech, consistently ranked as offering the highest quality services.
  • Fast delivery of comprehensive data reports ensuring critical decisions can be made with confidence and speed.

Highlights of DiscoverX Service Portfolio:

  • KINOMEscan® and PathHunter® Screening and Profiling Services are complete solutions for screening and profiling compounds using GPCR, Kinase, Epigenetic, NHR, Pathway, Interleukin, and Checkpoint targets to identify quality hits and follow up for potency, selectivity, or kinetics determination
  • SAFETYscan™ Services offer a broad menu of assays, including targets recommended by major Pharmaceutical companies (Bowes, et.al. 2012) to provide early assessment of the potential hazard of a compound or chemical series
  • BioMAP® Phenotypic Profiling Services provides drug developers over 60 human primary cell-based models of tissue and disease biology enabling profiling of compound effects across 100’s of clinically relevant protein biomarkers and comparing its performance and mechanistic behavior to over thousands of reference compounds

Selected Publications:

  • E Jacoby, et al. Extending kinome coverage by analysis of kinase inhibitor broad profiling data. Drug Discovery Today. 2015 Jun;20(6):652-8.
  • P Ciceri, et al. Dual kinase-bromodomain inhibitors for rationally designed polypharmacology. Nature Chemical Biology 2014;10, 305–312.
  • DK Treiber, NP Shah. Ins and outs of kinase DFG motifs. Chem Biol. 2013 Jun 20; 745-6
  • LM Wodicka, et al. Activation state-dependent binding of small molecule kinase inhibitors: structural insights from biochemistry. Chem Biol. 2010 Nov 24;17(11):1241-9.
  • K. A. Houck, et al. Profiling bioactivity of the ToxCast chemical library using BioMAP primary human cell systems. Journal of Biomolecular Screening, 2009.
  • E. L. Berg, et al. Characterization of compound mechanisms and secondary activities by BioMAP analysis. Journal of Pharmacological and Toxicological Methods, vol. 53, no. 1, pp. 67–74, 2006.

DiscoveRx today announced the acquisition of BioSeek®, a drug discovery services company based in South San Francisco, California. BioSeek's phenotypic screening technology improves the success rate of pharmaceutical research and development by integrating primary cell profiling into the drug discovery process. The proprietary BioMAP® systems incorporate primary human cell-based disease models that generate predictive biological activity profiles for potential drugs. As a result, the technology drives the selection and development of new drug candidates and safer chemicals. The acquisition represents another significant step in DiscoveRx's strategy to provide next generation drug discovery solutions through internal development and strategic acquisitions.

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Ligand Binding Assays
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Stable Cell Line Generation
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Kinase Assays
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KINOMEscan® Kinase Screening & Profiling Services

With over 15 years of experience in kinase drug discovery, DiscoverX offers the largest collection of nearly 500 biochemical and cell-based kinase assays. KINOMEscan, our novel kinase inhibitor binding platform, includes an industry leading set of kinase assays that allow... Show more »

KINOMEscan® Kinase Screening & Profiling Services

With over 15 years of experience in kinase drug discovery, DiscoverX offers the largest collection of nearly 500 biochemical and cell-based kinase assays. KINOMEscan, our novel kinase inhibitor binding platform, includes an industry leading set of kinase assays that allow rapid determination of kinome-wide compound potency and selectivity.

The KINOMEscan Services Offering:

-scanMAX® Kinase Assay Panel - Broadest Kinome Coverage for Selectivity Screening
-The scanMAX service leverages a panel of 468 kinases covering AGC, CAMK, CMGC, CK1, STE, TK, TKL, lipid and atypical kinase families, plus important mutant forms. scanMAX is an ideal choice for all stages of drug discovery and development, to identify the most selective drug candidates from the largest collection of wildtype kinase assays.

-KdELECT® Kinase Service - 5-Day Turnaround
-The KdELECT service measures compound potency to support your SAR analysis. Select from the largest collection of kinases, with the broadest dynamic range, KdELECT service allows you to differentiate high potency compounds down to 100pM level.

-scanELECT® Kinase Selectivity & Profiling Service – Ideal for Screening
-Select from KINOMEscan's collection of 468 kinase assays and build your own customized assay panel of any size comprising only of kinases relevant to your program. scanELECT is a flexible solution to meet your screening needs of any library size or any stage in the drug discovery pipeline.

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Cancer
Drug discovery
GPCR Screening and Profiling
Price on request

GPCR Screening & Profiling-GPCRscan®
Evaluate Compound Mechanism of Action or Binding Mode

The GPCR profiling service offers a collection of over 600 naturally coupled stable GPCR cell lines that enable multiple downstream signaling events to be studied in response to compound activation. Whether your focus is hit... Show more »

GPCR Screening & Profiling-GPCRscan®
Evaluate Compound Mechanism of Action or Binding Mode

The GPCR profiling service offers a collection of over 600 naturally coupled stable GPCR cell lines that enable multiple downstream signaling events to be studied in response to compound activation. Whether your focus is hit identification, selectivity profiling, or SAR optimization, these services can reduce the time and cost of GPCR drug discovery. Determine compound mechanism of action or choose a compound that shows ligand bias for development in the clinic.

GPCR Services

gpcrMAX and orphanMAX panels

The largest commercial panel of functional cell-based assays available gpcrMAX and orphanMAX contain 241 assays covering 60 distinct receptor families.
gpcrMAX and orphanMAX panels are an ideal choice to validate new targets or identify off target liabilities early so the best candiates can move into clinical trials

gpcrE/IC50ELECT℠

Quantitate compound potency against any GPCRscan functional cell-based assay. Agonist half maximal effective concentrations (EC50s) and antagonist half maximal inhibitory concentrations (IC50s) are calculated from duplicate 10-point dose-response curves. Measurements are made under optimized conditions to generate consistent and reproducible data that reliably reflects the drug’s potency.

gpcrELECT℠ Personalized Screening & Profiling

gpcrELECT provides you access to the world's largest menu of naturally coupled known, orphan and ortholog GPCR cell lines. Select your target in any one of our convenient assay formats – Arrestin recruitment, 2nd messenger signaling (cAMP or Calcium) or receptor internalization. Screen your compounds against a single GPCR target or design a customized panel of receptors to study off-target selectivity or screen or profile the same target in multiple modes for biased ligand discovery. gpcrELECT is an extremely flexible solution with multiple applications including, primary HTS & lead discovery and ongoing lead optimization & SAR.

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Human
Drug discovery
Hit to Lead and Lead Optimization
Price on request

DiscoverX Drug Discovery Services

Our portfolio of drug discovery services and screening solutions are designed to support you at every stage of development - from HTS & library profiling, to lead optimization and preclinical development. Powered by our EFC and competition binding technology platforms, we offer the largest... Show more »

DiscoverX Drug Discovery Services

Our portfolio of drug discovery services and screening solutions are designed to support you at every stage of development - from HTS & library profiling, to lead optimization and preclinical development. Powered by our EFC and competition binding technology platforms, we offer the largest portfolio of biochemical and functional cell-based assays. Reduce the time and cost of your discovery programs by taking advantage of our expertise and innovative technology platforms to find a solution that meets your project needs.

LeadHunter® Drug Discovery Services Portfolio:

  • Kinase Profiling - KINOMEscan® Kinase Screening & Profiling Services
  • Functional Cell-Based GPCR Assay Panel - GPCRscan® service
  • Off-target Safety Screening – Safety44™ Panel
  • Checkpoint Assay Service - iONCscan Screening and Profiling Services
  • Interleukin Screening and Profiling Services-ILscan Services
  • Epigenetic Profiling - BROMOscan® Bromodomain Assay Panel
  • Phenotypic Drug Discovery Portfolio – BioMAP® Phenotypic Screening Platform utilizing Broad and Translational Human Disease Models
    Pathway Profiling
    Nuclear Receptor (NHR) Profiling
    Therapeutic Panel Services
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In vitro Pharmacology Studies
Price on request

SAFETYscan™ In Vitro Pharmacological Profiling Services

In vitro pharmacological profiling is increasingly being used earlier in the drug discovery process to identify undesirable off-target activity profiles. SAFETYscan services offer a broad menu, including the assays recommended by major pharmaceutical companies (Bowes... Show more »

SAFETYscan™ In Vitro Pharmacological Profiling Services

In vitro pharmacological profiling is increasingly being used earlier in the drug discovery process to identify undesirable off-target activity profiles. SAFETYscan services offer a broad menu, including the assays recommended by major pharmaceutical companies (Bowes et.al. 2012). All SAFETYscan assays are human orthologs and provide functional data for efficient decision making, while other commercial panels are primarily binding assays for humans and rodents. Assessing the specificity of lead compounds early in development using highly relevant and predictive assays allows more informed decisions about compound safety, ultimately leading to the development of safer and more effective drugs.

SAFETYscan Services Offerings:

Safety44™ panel of functional assays with all human targets for safety screening are now available. Additionally, DiscoverX offers the broadest portfolio of GPCR and kinases targets to meet your safety screening needs.
SAFETYscan In Vitro Pharmacological Profiling Services Highlights:

-Better confidence using human target assays versus rodent orthologs
-Functional data to support SAR analysis in lead optimization
-Reduce expensive follow up studies

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Cancer
Drug discovery
neuroscience
hERG
Ion Channel Screening Assays
Price on request

SAFETYscan™ Ion Channel Services

Expanding our ion channel services to support safety pharmacology studies, we now offer SAFETYscan patch clamping service in addition to fluorescence ion channel assays. From manual patch clamp, the gold standard in ion channel work, to an automated platform, the SAFETYscan patch clamp service... Show more »

SAFETYscan™ Ion Channel Services

Expanding our ion channel services to support safety pharmacology studies, we now offer SAFETYscan patch clamping service in addition to fluorescence ion channel assays. From manual patch clamp, the gold standard in ion channel work, to an automated platform, the SAFETYscan patch clamp service provides efficient and high quality electrophysiology test for off-target activity of compounds.

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Compound Profiling
Price on request

DiscoverX contract screening and profiling services provides you flexible and convenient access to our technology and expertise to help drive your drug discovery programs. Outsource high-throughput screens, design custom panels by selecting from a comprehensive portfolio of targets, take part in our regularly scheduled panel runs,... Show more »

DiscoverX contract screening and profiling services provides you flexible and convenient access to our technology and expertise to help drive your drug discovery programs. Outsource high-throughput screens, design custom panels by selecting from a comprehensive portfolio of targets, take part in our regularly scheduled panel runs, or use our lead optimization services as a follow up study tool to support all your medicinal chemistry programs.

Add the convenience of outsourced profiling with consistent, high quality data and accelerated turnaround times to reduce the time and cost of your discovery programs while freeing up internal scientists to focus on other high value discovery activities.
Service Features & Benefits

  • Flexible access to all major drug targets, technology formats & assay readouts
  • Reliable and quick turnaround times (5-20 business days depending on the assay type)
  • Convenient on line ordering and integrated invoicing, standardized globally
  • Customized projects and dedicated FTE’s to expand your capacity and throughput
  • Integration with custom assay development to provide novel solutions
  • Secure access to your data and a powerful visualization tool for better decision making and publication quality data

BioMAP Phenotypic Profiling Services
BioMAP Phenotypic Profiling Services provides drug developers a simple, in vitro solution for gathering the most comprehensive insights about efficacy and safety of a compound or lead molecule. With over 60 human primary cell-based models of tissue and disease biology to choose from, you can profile your compound‘s effects across 100’s of clinically relevant protein biomarkers and compare its performance and mechanistic behavior to over thousands of reference compounds. The end result is physiologically relevant insights into the efficacy, safety, and mechanism of action of your candidate drug molecule to accelerate the time from testing to therapy.

Highlights:
Human targets for data relevant to human biology ƒ
Prioritize candidates prior to in vivo experiments or IND submission ƒ
Test compounds for safety, efficacy, and better understanding of mechanism of action ƒ
Inform preclinical design and biomarker selection for smooth transition into trials ƒ
Manage product lifecycle and reposition existing drugs by finding better efficacy through combinations and novel indications

Diversity PLUS:
Test agenst across a broad range of human disease models
Take advantage of a database of over 4,000 reference compounds to better predict clinical indication, safety and mechanism of action

Oncology Tumor Microenvironment Panels:
The perfect assay to gain insight into the efficacy of Immuno-Oncology and Oncology drugs and compounds
Fibrosis Panels and T Cell/Autoimmune Panels
Take a deeper look at agent effects across a different disease-focused panels
60+ Additional individual human primary cell models of disease

Fibrosis Panel

The BioMAP Fibrosis Panel models fibrotic diseases, wound healing and extracellular matrix remodeling under inflammatory and pro-fibrotic conditions. The systems in this panel are comprised of human primary fibroblasts cultured alone or co-cultured with either primary renal or small airway epithelial cells to allow the measurement of drug or test agent effects on protein biomarkers. The biomarker activity profiles can be compared to the profiles of selected clinical drugs and reference compounds from BioMAP database to gain insights into mechanism of action, efficacy and safety-related effects of test agents.

Drug Discovery Applications

  • Investigation of effects of the test agent on fibrosis, wound healing, matrix remodeling and kidney and lung biology
  • Biological mechanism of action, efficacy and safety
  • Biomarker discovery
  • Benchmarking to clinical drugs and reference compounds

Fibrosis Panel Advantages

Select benchmark compounds exhibited differential modulation of readouts across MyoF, REMyoF and SAEMyoF systems, indicating the key influence of the tissue-specific epithelia on the fibrotic disease biology
Common protein biomarker readouts across REMyoF and SAEMyoF systems enable comparison of effects on organ-specific fibrosis and inflammation models
Screening in one or more Fibrosis systems could be used to differentiate compounds and help guide indication selection

Service

Test agent (chemical compound or biologic) profiling in selected BioMAP Systems
4 concentrations in triplicate

Description

Annotation Identification and biological interpretation of relevant BioMAP biomarker activities (readouts)
Benchmarking Direct comparison of test agent to a specified clinical drug or reference compound from BioMAP database (list available upon request)

Deliverables

Study Report BioMAP profile plots, annotation of activities with respect to biological significance, graphical overlays of test agent and drug or reference compound profiles, expert data interpretation and analysis
Data Report Log ratio data tables, table of cytotoxicities, table of active biomarker readouts

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Cell-Based Compound Screening
Price on request

Enjoy the convenience of outsourced high-throughput screening with ready access to a portfolio of more than 1000 highly validated and robust assays that can be deployed for testing against compound collections of any size. Outsource with confidence knowing that our assays are performed under highly standardized conditions using... Show more »

Enjoy the convenience of outsourced high-throughput screening with ready access to a portfolio of more than 1000 highly validated and robust assays that can be deployed for testing against compound collections of any size. Outsource with confidence knowing that our assays are performed under highly standardized conditions using best-in-class lab automation to provide exceptional assay performance and reproducible data. Our library screening services save you time and frees up internal resources to focus on other high value discovery activities. From single target HTS to library profiling and follow-up services, DiscoveRx delivers high quality data that ensures project timelines are met and maximizes the discovery of potent & selective lead compounds for new drug discovery programs.

Library Screening Features and Benefits

  • Comprehensive menu of over 1000 validated assays
  • Capacity to screen more than 100,000 wells/day
  • Flexible: screen any number of compounds against any number of targets
  • Accurate, precise & reproducible data
  • Rapid data turnaround
  • Identify potent and selective high quality leads in a single screen
  • Identify new target opportunities from existing chemical assets
  • Free internal resources to focus on other activities

Accurate, Precise & Reproducible Data

LeadHunter Discovery Services partners have long recognized that accurate, precise and reproducible data are critical components of the discovery and development process and are the cornerstone of a successful screening partnership. All our assays undergo thorough validation prior to being released into production and assay performance is continually monitored to ensure accurate pharmacology and data consistency.

Learn what our customers are saying in peer reviewed publications:

  • KINOMEscan team and from our partners
  • PathHunter Arrestin
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In vitro Drug Safety Evaluation
Price on request

SAFETYscan™ In Vitro Pharmacological Profiling Services

In vitro pharmacological profiling is increasingly being used earlier in the drug discovery process to identify undesirable off-target activity profiles. SAFETYscan services offer a broad menu, including the assays recommended by major pharmaceutical companies (Bowes... Show more »

SAFETYscan™ In Vitro Pharmacological Profiling Services

In vitro pharmacological profiling is increasingly being used earlier in the drug discovery process to identify undesirable off-target activity profiles. SAFETYscan services offer a broad menu, including the assays recommended by major pharmaceutical companies (Bowes et.al. 2012). All SAFETYscan assays are human orthologs and provide functional data for efficient decision making, while other commercial panels are primarily binding assays for humans and rodents. Assessing the specificity of lead compounds early in development using highly relevant and predictive assays allows more informed decisions about compound safety, ultimately leading to the development of safer and more effective drugs.

SAFETYscan Services Offerings:

Safety44™ panel of functional assays with all human targets for safety screening are now available. Additionally, DiscoverX offers the broadest portfolio of GPCR and kinases targets to meet your safety screening needs.
SAFETYscan In Vitro Pharmacological Profiling Services Highlights:

-Better confidence using human target assays versus rodent orthologs
-Functional data to support SAR analysis in lead optimization
-Reduce expensive follow up studies

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Drug Target Identification
Price on request
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Drug Mechanism of Action Studies
Mechanism of Action Studies
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Drug Target Validation
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Drug Discovery
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Biomarker Discovery
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Receptor Deorphanization
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Signaling Pathway Analysis
Price on request

DiscoverX® Discovery Services provides you access to a comprehensive collection of cell-based pathway indicator assays designed to detect activation or inhibition of complex signal transduction pathways in response to compound treatment. Based on the proven PathHunter® technology, these stable cell lines allow you to measure... Show more »

DiscoverX® Discovery Services provides you access to a comprehensive collection of cell-based pathway indicator assays designed to detect activation or inhibition of complex signal transduction pathways in response to compound treatment. Based on the proven PathHunter® technology, these stable cell lines allow you to measure distinct events within a variety of pathways involved in compound toxicity, cholesterol metabolism, antioxidant pathways, DNA damage, ER stress and many others to drive your lead discovery and optimization without relying on reporter gene assays or complex phenotypic screens.
Comprehensive Menu

PathHunter® Pathway Assays are novel, non-imaging functional whole cell assays for measuring multiple cellular signaling events such as protein translocation, secretion, degradation and expression using highly sensitive and quantitative chemiluminescent detection. This approach can be applied to any cell signaling cascade in which a target protein either degrades, translocates or is secreted. These assays serve as pathway indicators.

Panel Highlights and Benefits

  • Functionally validated cell lines – high quality results every time
  • Single functional readout – applicable to different kinase receptors and families
  • High quality, reproducible data
  • Superior assay windows & robust performance (Z' > 0.6)
  • Agonist and antagonist modes available
  • Broad applications – specificity and compound toxicity/liability
  • Rapid data turnaround time
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Nuclear Receptor Assays
Price on request

Nuclear Hormone Receptor Screening & Profiling-NHRscan ℠
Measure NHR Translocation or Co-Activator Interaction

Nuclear Hormone Receptor Screening and Profiling Services offers over 18 different cellular assays that monitor receptor activation by either nuclear translocation or NHR co-activator interaction. These services... Show more »

Nuclear Hormone Receptor Screening & Profiling-NHRscan ℠
Measure NHR Translocation or Co-Activator Interaction

Nuclear Hormone Receptor Screening and Profiling Services offers over 18 different cellular assays that monitor receptor activation by either nuclear translocation or NHR co-activator interaction. These services can be used to profile compounds against a broad panel of assays for identification of new targets or determination of safety or selectivity of potential drug candidates. They can also be used to determine potency or rank ordering of potential hit or lead molecules.

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Drug discovery
Bromodomain Assays
Price on request

BROMOscan® Epigenetic Screening & Profiling Services

DiscoverX offers a suite of assays for epigenetic targets to enable identification and classification of inhibitors based on potency, selectivity and in vitro efficacy. BROMOscan, is an industry leading collection of 40 validated, quantitative binding assays for... Show more »

BROMOscan® Epigenetic Screening & Profiling Services

DiscoverX offers a suite of assays for epigenetic targets to enable identification and classification of inhibitors based on potency, selectivity and in vitro efficacy. BROMOscan, is an industry leading collection of 40 validated, quantitative binding assays for identifying small molecule bromodomain inhibitors. Bromodomains are epigenetic “reader” proteins that have emerged as an important new druggable target class in small molecule inhibitor drug discovery with several bromodomain-containing proteins being associated with disease.

bromoMAX℠ - Largest Bromodomain Assay Panel

With a panel of 32 bromodomain assays, bromoMAX includes the entire BET family along with other diverse bromodomains including ATAD2, BAZ2A, CREBBP, SMARCA2, PBRM1(5), TAF1L and others. This comprehensive collection of validated assays virtually eliminates assay development bottlenecks and provides a comprehensive selectivity screening tool that can accelerate the identification and optimization of potent and selective small molecule bromodomain inhibitors.

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Custom Biochemical Assay Development
Price on request

BCL2scan: Biochemical ligand binding assays for anti-apoptotic BCL2 family members

Biochemical Ligand Binding Assays for BCL2, BCLXL, BCLW, BCL2A1 and MCL1

DiscoverX has developed a best in class comprehensive panel of quantitative biochemical ligand binding assays for anti-apoptotic BCL2 family proteins that can be used to... Show more »

BCL2scan: Biochemical ligand binding assays for anti-apoptotic BCL2 family members

Biochemical Ligand Binding Assays for BCL2, BCLXL, BCLW, BCL2A1 and MCL1

DiscoverX has developed a best in class comprehensive panel of quantitative biochemical ligand binding assays for anti-apoptotic BCL2 family proteins that can be used to identify and to optimize therapeutic small molecule inhibitors targeting proteins from this target class. The BCL2 family of proteins determine the commitment of cells to apoptosis and are emerging therapeutic targets for oncology, auto-immune disease, neurodegeneration, and aging-related morbidities. The BCL2 family consists of pro-apoptotic and anti-apoptotic members that interact to regulate the MOMP pathway to apoptosis. The anti-apoptotic family members are the focus of many drug discovery efforts and include BCL2, BCLXL, BCLW, BCL2A1 and MCL1.

The DiscoverX BCL2 family assays enable measurement of potency and selectivity across these anti-apoptotic family members. You can screen, profile and measure Kd values in one flexible format in both high or low-throughput modes.

Platform Benefit & Technology Details

  • Menu of fully validated quantitative ligand binding assays for anti-apoptotic BCL2 family targets
  • Measure potency and selectivity in the same assay format enabling inter-protein inhibitor potency rank-ordering - and SAR
  • Screen, profile and measure Kds in one flexible format in high and low-throughput modes
  • Broad dynamic range for accurate affinity measurements (tight binding limit <70 pM)

How the Technology Works

Compounds interacting with the BCL2 family protein prevent binding of these proteins to an immobilized known peptide ligand and reduce the amount of target protein captured on the solid support (Panels A & B). Conversely, test molecules that do not bind the BCL2 family protein have no effect on the amount of target protein captured on the solid support (Panel C). Screening “hits” are identified by measuring the amount of BCL2 family protein captured in test versus control samples by using a quantitative, precise and ultra-sensitive qPCR method that detects the associated DNA label (Panel D). In a similar manner, dissociation constants (Kds) for test compound-BCL2 family protein interactions are calculated by measuring the amount of target protein captured on the solid support as a function of the test compound concentration. Assay conditions are optimized for the measurement of true thermodynamic test compound Kd values.

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Scale-up Production
Price on request

DiscoverX offers a convenient bulk cell scale up option with the purchase of any PathHunter® or cAMPHunter™ clone. Package sizes range from 100 million to 10 billion cells. Cells are functionally tested for optimal assay performance and are confirmed to be Mycoplasma-free. Combine frozen, assay-ready bulk cells with PathHunter®... Show more »

DiscoverX offers a convenient bulk cell scale up option with the purchase of any PathHunter® or cAMPHunter™ clone. Package sizes range from 100 million to 10 billion cells. Cells are functionally tested for optimal assay performance and are confirmed to be Mycoplasma-free. Combine frozen, assay-ready bulk cells with PathHunter® certified Reagents and Control Ligands to expedite your screening campaigns and ensure optimal and reproducible results every time.

Rapidly growing use of cells for high-throughput screening (HTS) has posed a number of challenges including batch performance variation, cell production scheduling, capability and capacity management.

DiscoverX offers the solution – a convenient scale up service for any PathHunter®, cAMP Hunter™ or InCELL Hunter™ cell line. We provide single batches of cells in custom package sizes ranging from 50 million to 10 billion cells, quantities sufficient for complete screening campaigns, effectively decoupling cell production from screening. These cells undergo the same rigorous testing as our portfolio products, i.e., they are functionally tested to ensure optimal performance and stability, and are guaranteed to be Mycoplasma free.

Ensure speed and success in your screening campaign through the use of our single batch cell production service.
Key Features and Advantages

  • Single batch production, removing batch performance variance
  • On demand, custom scale production up to 10 billion cells
  • Top quality cells, validated for optimal performance
  • No additional resources required
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Custom Cell-Based Assays
Price on request

When existing off-the-shelf solutions are not enough, trust DiscoverX to develop a solution that will produce the quality results you expect. DiscoverX offers a range of custom development services including bioassays for drug discovery, process development and basic research. Our team of molecular and cellular biologists will... Show more »

When existing off-the-shelf solutions are not enough, trust DiscoverX to develop a solution that will produce the quality results you expect. DiscoverX offers a range of custom development services including bioassays for drug discovery, process development and basic research. Our team of molecular and cellular biologists will work with you to match one of our proprietary platforms such as PathHunter®, KINOMEscan®, InCell Hunter® or HitHunter® to deliver a cellular or biochemical solution that provides the quality results you expect.

Available Services

  • Orthologs and mutants
  • Custom cell backgrounds
  • Custom cell lines
  • Protein overexpression
  • Kit formulation/packaging

PathHunter® Cell-based Assay Development Services

PathHunter is a versatile cell-based assay platform, based on our proprietary EFC technology, successfully employed to develop validated assay solutions for over 300 targets spanning many target classes including GPCRs, Kinase, NHRs, and more. Innovative application of this core technology, has enabled the development of robust, reliable, and high-throughput-friendly chemiluminescent assays to measure protein-protein interaction, protein translocation and degradation.
PathHunter CAD Services will work closely with you to develop a custom cell-based assay specific for your target, providing comprehensive solutions where you need them most.
PathHunter - cell-based technology format can query many types of interactions

  • Measure inducible and constitutive protein interactions
  • Measure protein translocation to various intracellular locations in a
  • non-imaging HTS friendly assay
  • Accurately measure protein levels and stabilization

Engineered Cell Lines

A flexible approach to customized cell line generation
Using proprietary DiscoveRx vectors and viral transduction techniques, we can build custom engineered cell lines specific to your project requirements in as little as 6-8 weeks. Need a modified cell background for an existing catalog product, mutant or ortholog? These can be fast-tracked and in certain cases developed in as little as 3 weeks from project acceptance.
Capabilities

  • Cell background variants
  • Mutant or orthologs of existing catalog products
  • Protein overexpression
  • Bulk cell-scale up

HitHunter® Biochemical Assay Development Services

The HitHunter Assay is a validated platform for biochemical quantification of second messenger signaling for cAMP and cGMP. Extension of the HitHunter principle to novel targets allows for investigation of protease and substrate activity. HitHunter assays are formatted as competitive immunoassays, where analytes are detected in solution, without separation or wash steps, providing improved sensitivity and precision compared to conventional immunoassays.

HitHunter Biochemical Assay Development Services will work closely with you to develop a custom assay to detect protease and substrate activity or measure an analyte, providing comprehensive solutions where you need them most.
HitHunter biochemical technology format has these advantage

  • Develop novel assays for identifying analytes
  • Develop cleavage assays to assay protease activity
  • Develop assays for use with primary cells, etc
  • All assays will be HTS compatible, etc.
  • Chemiluminescent detection - minimizes false positives from auto fluorescence
  • Reliable and robust assay with high Z' (0.75)
  • HTS-friendly - adaptable to 1536 applications
  • Large assay windows
  • Broad application to multiple target classes

InCell Hunter™ Assay Development Services

InCELL Hunter is a novel, yet powerful in vitro cellular ligand binding assay platform that can be used to determine compound binding in a native cellular environment. InCELL Hunter uses enzyme fragment complementation technology to measure binding to the protein of interest and is a powerful development tool for intractable targets where other technologies have been unsuccessful.
InCell Hunter – A simple cell-based assay for complex targets

  • Cell-based assay broadly applicable to several target classes
  • Target-specific response
  • Direct measure of intracellular binding to target
  • Activity independent
  • HTS compatible
  • Bridge between in vitro binding assay and cellular functional assay
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Compound Profiling in Cancer Cell Panels
Price on request

BioMAP Oncology and Immune Oncology PanelsSystems consist of complex co-cultures of PBMCs pooled from healthy donors with early passage human primary fibroblasts or endothelial cells, with or without a tumor cell line. These systems are stimulated to recapitulate and model tumor-immune-stromal, immune-stromal,... Show more »

BioMAP Oncology and Immune Oncology PanelsSystems consist of complex co-cultures of PBMCs pooled from healthy donors with early passage human primary fibroblasts or endothelial cells, with or without a tumor cell line. These systems are stimulated to recapitulate and model tumor-immune-stromal, immune-stromal, tumor-immune-vascular or immune-vascular microenvironments and disease relevant signaling pathways that manifest during tumorigenesis. By measuring and analyzing biomarker activities and/or comparing the resulting activity profiles of a test agent to the profiles of selected reference compounds or drugs, one can gain insight into the mechanism of action, efficacy and safety-related effects.

Drug Discovery Applications

  • Biological mechanism of action, efficacy and safety
  • Biomarker discovery
  • Benchmarking to clinical drug or reference compound
  • Testing of drug or compound combinations
  • Oncology and Immune Oncology Panel Advantages

PBMCs, human primary fibroblasts or endothelial cells, cancer cell lines

  • Microenvironment - immune, vascular or stromal, tumor
  • Additional custom protein biomarker readout options

Service

  • Test agent (chemical compound or biologic) profiling in selected BioMAP Systems
  • 4 concentrations in triplicate

Description

  • Annotation Identification and biological interpretation of relevant BioMAP biomarker activities (readouts)
  • Benchmarking Direct comparison of test agent to a specified clinical drug or reference compound from BioMAP database (list available upon request)

Deliverables

  • Study Report BioMAP profile plots, annotation of activities with respect to biological significance, graphical overlays of test agent and drug or reference compound profiles, expert data interpretation and analysis
  • Data Report Log ratio data tables, table of cytotoxicities, table of active readouts
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Compound Profiling in Immune Cell Panels
Price on request

The T Cell Autoimmune Panel includes BioMAP systems comprised of complex co-cultures of immune and adherent human primary cell types. These cell-based assay systems model adaptive immune cell microenvironment and T and B cell responses in a platform amenable to compound screening. BioMAP technology measures physiologically... Show more »

The T Cell Autoimmune Panel includes BioMAP systems comprised of complex co-cultures of immune and adherent human primary cell types. These cell-based assay systems model adaptive immune cell microenvironment and T and B cell responses in a platform amenable to compound screening. BioMAP technology measures physiologically relevant biomarker readouts (proteins, small molecule mediators, etc.), which permits insight into biological mechanism of action, efficacy and safety related effects of compounds.

Service

  • Compound profiling (chemicals or biologics) in selected BioMAP Systems
  • 4 doses in triplicate

Description

  • Annotation Identification and biological intrepretation of relevant BioMAP activities
  • Benchmarking Direct comparison of test agent to six standard-of-care reference compounds (Cyclosporine A, - - Everolimus, Ibrutinib, Idelalisib, Prednisolone, Tofacitinib)

Deliverables

  • Study Report BioMAP profile plots, annotation of activities with respect to biological significance, graphical overlays of test and standard-of-care reference compound profiles and cluster analysis (measure of overall similarity) of test compound with standard-of-care reference compounds
  • Data Report Log ratio data tables, 95% significance envelope, table of cytotoxicities, table of active readouts
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Assay Development
Price on request

Depending on the specific research needs, our scientists will develop new BioMAP Systems to supplement the existing collection of BioMAP Systems. With the extensive expertise in building complex assays with human primary cells, we can rapidly develop and validate new assays specifically tailored to meet the goals of your discovery... Show more »

Depending on the specific research needs, our scientists will develop new BioMAP Systems to supplement the existing collection of BioMAP Systems. With the extensive expertise in building complex assays with human primary cells, we can rapidly develop and validate new assays specifically tailored to meet the goals of your discovery programs. The human primary cell types that have been validated at BioSeek for assay development include:

Peripheral blood mononuclear cells
B-cells
Th0, Th1 and Th2 T cells
Monocytes
M1 macrophages
M2 macrophages
Neutrophils
Foam cells
Venular endothelial cells (umbilical cord)
Arterial endothelial cells (coronary artery)
Microvascular endothelial cells (brain)
Microvascular endothelial cells (dermal)
Sinusoidal endothelial cells (liver)
Arterial smooth muscle cells (coronary artery)
Venular smooth muscle cells (umbilical vein)
Dermal fibroblasts (foreskin)
Lung fibroblasts
Lung myofibroblasts (derived from lung fibroblasts)
Liver fibroblasts
Intestinal myofibroblasts
Bronchial epithelial cells
Intestinal epithelial cells
Proximal tubular epithelial cells
Skeletal muscle cells
Mesenchymal cells
MSC-derived osteoblasts, chondrocytes and adipocytes
Visceral and subcutaneous preadipocytes
Astrocytes
Hepatocytes

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Multi-Disease Gene Panel
Price on request

BioMAP® Diversity PLUS Panel

Diversity PLUS consists of 12 BioMAP Systems containing early passage primary human cell types from multiple tissues that represent a broad range of human biology relevant to multiple therapeutic areas. Cells are cultured alone or as co-cultures and stimulated with a combination of factors to... Show more »

BioMAP® Diversity PLUS Panel

Diversity PLUS consists of 12 BioMAP Systems containing early passage primary human cell types from multiple tissues that represent a broad range of human biology relevant to multiple therapeutic areas. Cells are cultured alone or as co-cultures and stimulated with a combination of factors to recapitulate the multi-component signaling networks associated with disease states. Compounds are interrogated in these systems to determine their phenotypic impact on physiologically relevant disease biology in terms of efficacy and safety.

Drug Discovery Applications

  • Translational biology for drug discovery
  • Phenotypic drug discovery
  • Competitive analysis and comparison to clinical standards of care
  • Analysis of compounds with novel mechanisms of action
  • Biomarker discovery

Diversity PLUS Advantages

  • Predictive – Regulatory and feedback mechanisms
  • Reproducible – Fully automated platform
  • Efficient – Hundreds of targets and mechanisms are interrogated simultaneously
  • Powerful – Comparison of test compounds to large database
  • Meaningful – Correlation of BioMAP responses to clinical data

Service

  • Compound profiling (chemicals or biologics) in 12 BioMAP Systems
  • 148 readouts at 4 concentrations

Description

Annotation Identification and biological intrepretation of relevant BioMAP activities
Similarity Search Results from unsupervised search for a biologically similar compound from the BioMAP reference database of >3000 compounds, biologics, approved drugs and experimental agents
Benchmarking Direct comparison of test agent to a specified reference compound from the BioMAP Database

Deliverables

  • Study Report BioMAP profile plots, annotation of activities with respect to biological significance, graphical overlays of test and reference compound profiles, results of similarity search, expert data interpretation and collaborative analysis
  • Data Report Log ratio data tables, 95% significance envelope, table of cytotoxicities, table of active readouts
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Pharmacochaperone Trafficking Assays
Price on request
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Interleukin Screening and Profiling
Price on request

Interleukin Screening & Profiling- ILscan

Discover Molecules that Activate or Inhibit Interleukin Receptors
DiscoverX offers cell-based assay services for interleukins that are highly specific and can be used to identify molecules that activate or inhibit Interleukin receptors and enable rapid development of drugs... Show more »

Interleukin Screening & Profiling- ILscan

Discover Molecules that Activate or Inhibit Interleukin Receptors
DiscoverX offers cell-based assay services for interleukins that are highly specific and can be used to identify molecules that activate or inhibit Interleukin receptors and enable rapid development of drugs targeting immune-system disorders. These assays can be used to determine potency or rank ordering of potential hit or lead molecules.

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Predictive toxicology
Price on request

During compound profiling, BioMAP analysis can identify unexpected drug activities and potential toxicities at an early stage in discovery. This knowledge will inform and complement animal studies, but most importantly it will provide insight into the safety-related effects of compounds on human biology before more costly stages... Show more »

During compound profiling, BioMAP analysis can identify unexpected drug activities and potential toxicities at an early stage in discovery. This knowledge will inform and complement animal studies, but most importantly it will provide insight into the safety-related effects of compounds on human biology before more costly stages of development. Assays based on cell lines and simpler cell –based models can miss these findings.

BioMAP Systems can detect and distinguish a range of biological mechanisms associated with cellular and specific human toxicities, including those that are difficult to detect or de-convolute by target-based assays such as cell dysfunction (stress response), immunosuppression: B cell, T cell, mitochondrial dysfunction, microtubule function and many more.

We have been working since 2007 with the EPA as a contractor for the EPA's ToxCastTM program. EPA launched the ToxCast™ program to develop ways to predict potential toxicity of chemicals and to develop a cost-effective approach for prioritizing the thousands of chemicals that need toxicity testing.

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Immunotherapy Screening
Price on request

iONCscan Services for Checkpoint Receptors- iONCscan

Evaluate Potency, Stability, or Rank Order Drug Molecules
DiscoverX’s iONCscan services offer an expanding menu of cell-based assays that can enable screening & potency determination for new immunotherapy drugs. Assays include signaling assays for PD1, OX40, CD137 and an... Show more »

iONCscan Services for Checkpoint Receptors- iONCscan

Evaluate Potency, Stability, or Rank Order Drug Molecules
DiscoverX’s iONCscan services offer an expanding menu of cell-based assays that can enable screening & potency determination for new immunotherapy drugs. Assays include signaling assays for PD1, OX40, CD137 and an expanding menu of targets. Assays are specific and sensitive and most show biologically relevant responses and can be used for the discovery of small molecules or biologics.

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Safety Pharmacology & Efficacy Testing
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Lead Identification and Validation
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Enzyme Assays
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Computational Modeling
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Hit Identification
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Cellular Ion Channel Assays
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Process Consulting
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Drug Discovery & Development
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Cell-Based Assays
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Medicinal Chemistry
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DNA
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Biomarkers
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Receptor Assays
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Pharmacology & Toxicology
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Gene Panel Testing
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Cell-Based Screening Methods
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Biology
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Experimental Design
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Immunoassays
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Cells and Tissues
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Protein Services
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Project Management, Consulting, & Support Services
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Antibody Services
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Nucleic Acid Services
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Biochemistry & Molecular Biology
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Biomolecular Interaction Analysis
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Bioanalysis
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Specialized Cell-Based Assays
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Cell Signaling Analyses
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Biochemical Assays
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Epigenetic Analysis
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Mammalian Cell Culture
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2018-01-26 13:57:41 -0800

Net Promoter Score of 10 received for Kinase Assays.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2017-12-21 06:14:42 -0800

Net Promoter Score of 8 received for GPCR Screening and Profiling.

Additional Ratings: satisfaction with deliverable: 8, satisfaction with timeliness: 8.
2017-06-13 10:15:30 -0700

Net Promoter Score of 10 received for Nuclear Receptor Assays.

Additional Ratings: satisfaction with deliverable: 7, satisfaction with timeliness: 9.
2017-05-25 13:20:16 -0700

Net Promoter Score of 9 received for GPCR Screening and Profiling.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 9.

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