EUPROTEIN INC. offers custom development and production of high-quality bioresearch reagents, with a particular focus on human and other mammalian proteins, as well as recombinant monoclonal antibodies. In support of biopharmaceutical drug discovery and development, EUPROTEIN, Inc. develops and implements primarily the mammalian cell-based technology platforms for custom protein and monoclaonal antibody production services. Our complete US-New Jersey based laboratories with skilled scientists and customer service professionals accommodate your requirements for premium quality, fast delivery and effective communication.
To contribute to the global effort of combating the virus, EUPROTEIN has developed new products of the SARS-CoV-2 and the associated cellular receptor protein products. These high-quality proteins are all expressed in Human cell, can be used for basic research study of 2019-nCoVs, and would also help in potential diagnostic and therapeutic developments for the SARS-CoV-2 and related COVID-19 diseases.
Large quantity can also be available per request. Please do let us know and we'll provide bulk discount.
EUPROTEIN offers a variety of recombinant protein expression services ranging from protein engineering, production, and purification at various scales. We specialize in protein services with mammalian expression systems, primarily Human cells and Chinese Hamster Ovary (CHO) cells.
Mammalian cells produce protein products with unique advantages of correct folding, proper post-translational modification, and native or the most near-native conformation. At EUPROTEIN, we have been successful in producing several thousand proteins in mammalian cells, and offering custom services at different scales that fit your project needs.
EUPROTEIN provides customers with integrated process solutions from customer-driven project designs, gene synthesis, molecular biology services, protein expression, to protein purification and analytical characterizations.
We can start a pilot expression study of your protein project with as low as $498!
1) What service does this include?
Unless we know the expression level and yield of recombinant proteins and antibodies, we would start standard pilot expression service with human 293 cells, this covers gene synthesis, construction and expression/purification assessment, and will deliver all the protein obtained from pilot study if it can be purified. Expi293 or ExpiCHO is used for secreted expression of recombinant proteins in serum free medium. EUPORTEIN's own custom expression vector platform with the optimized designs will be used for the secreted expression and affinity purification such as His, Fc as well as other affinity tags. Due to the variable length of target genes, the actual price of pilot expression will primarily depend upon actual length or the number of base pairs. If the target genes are under <500 bp, for example, the VH, VK domains, scFv fragments or SdAb of the recombinant antibodies, there will not be extra charge for pilot expression. If the genes or proteins are big, extra charges might apply to cover cost of molecular biology. Also please note that particular analytical assays such as Endotoxin measurement or HPLC assessment etc are at extra cost and NOT covered under this.
2) What's the scale of the expression? How much protein and what purity would you provide
Typically the pilot expression study start 100~200 mL. If successful, 0.1mg to 5mg of purified recombinant proteins or monoclonal antibodies can be delivered. After pilot expression, if more materials is demanded, process will be implemented for producing protein/antibodies based upon desired volume of up to 20Liters, or the desired quantity of 200 ug to 100 mg, or even a few hundred mgs for particular proteins. Depending upon each individual protein, the purity of final deliverables should be 85% to 95%, based upon SDS-PAGE analysis.
3) What's your service, pricing and timeline for up to 1000ml scale expression?
The pricing for desired quantity can be different from protein to protein, however, it would be more standardized for the volume based expression. 1L scale of Expi293 expression is available.
4) Where will the work be actually done?
We are complete US-New Jersey based company, and our laboratories are physically located in NJ Bioscience Center at North Brunswick, New Jersey. All the work are done in the US. Site visit is available per request of reservation.
At EUPROTEIN, we understand the importance of recombinant antibodies in the discovery and development of novel biologic therapies. Therefore, we have developed the skillset to generate customized antibodies that show specialized binding properties, optimal half-lives and desirable effector functions, in support of your drug discovery efforts. Our offerings include:
- Hybridoma sequencing to identify variable regions of antibody heavy chain (VH) and the light chain (VL) from mouse, rabbit, and human clonal cells
- Antibody engineering for single chain, chimeric, and bispecific antibody products
- Assembly of full-length IgG constructs for antibody expression and production
- Research-grade antibody biosimilar expression and production in human or CHO cells
- Reverse engineering of monoclonal antibody through de novo antibody sequencing
Additional custom services can be requested for antibody purification from non-recombinant sources, antibody isotyping and antibody conjugation labelling.
EUPROTEIN offers recombinant monocloanl antibody services ranging from antibody engineering, expression construct design, production, and purification in flexible scales. We specialize in utilizing mammalian expression systems, primarily Human 293 and Chinese Hamster Ovary (CHO) cells to produce customized antibody.
At EUPROTEIN, we have been successful in producing over a thousand of recombinant murine and human monoclonal antibodies with serum-free 293 and CHO cell culture, and offering custom services at different scales and quality levels that fit your project needs.
EUPROTEIN offers a variety of recombinant protein expression services ranging from protein engineering, production, and purification services at various scales. We specialize in protein services in mammalian expression systems, primarily Human cells and Chinese hamster ovary (CHO) cells.
While the bacteria expression system is rapid and simpler to scale up, mammalian cells render unique advantages of correct folding, proper post-translational modification, and native or the most near-native conformation. At EUPROTEIN, we have been successful in producing several thousand proteins in mammalian cells, and offering custom services at different scales that fit your project needs.
In addition, EUPROTEIN provides customers with integrated process solutions from customer-driven project designs, molecular biology services, protein production, to purification and analytical characterizations.
EUPROTEIN has mastered the art of stable cell line development, in particular cell lines dedicated for assay development or high-level expression and production of recombinant monoclonal antibodies. The GS or DHFR expression system is the most widely used expression system in industry. Through serving our customers, we are able to bring you with a decade years of experience in GS and DHFR based stable cell line generation and upstream process development.
At EUPROTEIN, we have successfully developed over 30 stable CHO and other mammalian cell lines for research and early stage of biologic development. In addition to cell line development services, EUPROTEIN also offers the scale-up bioproduction using stable cell pools and selected stable cell lines.
Protein purification is very critical for the characterization of the function, structure, and interactions of proteins. Separation of the protein of interest from other impurities, is typically the most challenging aspect of protein purification. Through the complexicity of proteins we have previously worked with, we have acquired the skills in downstream purification processes to specific custom cases. Insoluble proteins can also be purified and refolded to the native state. We routinely purify proteins using affinity chromatography, ion-exchange, size-exclusion, hydrophobic interaction and other techniques.
EUPROTEIN develops and produces ten thousand of recombinant proteins from transient expression of DNA sequences or genes of different species, primarily human, mouse, rat, and monkeys genes.
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