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ES Cell Targeting Core

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San Francisco, California, US

About ES Cell Targeting Core

The UCSF ES Cell Targeting Core provides high-quality ES cell services with a high probability of germline transmission. The core offers ES cell targeting, genomic DNA extraction from 96-well plates, expansion of targeted ES cells, chromosome counts, and preparation of ES cells for microinjection, to all UCSF... Show more »

The UCSF ES Cell Targeting Core provides high-quality ES cell services with a high probability of germline transmission. The core offers ES cell targeting, genomic DNA extraction from 96-well plates, expansion of targeted ES cells, chromosome counts, and preparation of ES cells for microinjection, to all UCSF investigators as well as external investigators.

Prior to initiation of a project, consultation is available from the director on the entire procedures of generating knockout mice. This meeting will outline specifics of the project including knockout strategy, KO construct design, screening assays, and other issues relevant to microinjection and chimera breeding.

We work with the Gladstone Transgenic Gene Targeting Core for your microinjections to deliver a full-range gene targeting service. On the day of injection, we prepare cells and deliver them to Gladstone.

Recent publications by Core Facility users:

  • Theodoris C et al. Human disease modeling reveals integrated transcriptional and epigenetic mechanisms of NOTCH1 Haploinsufficiency. Cell 2015.
  • Ye L et al. Seamless modification of wild-type induced pluripotent stem cells to the natural CCR5d32 mutation confers resistance to HIV infection. PNAS 2014.
  • Cunningham JT et al. Protein and nucleotide biosynthesis are coupled by a single rate-limiting enzyme, PRPS2, to drive cancer. Cell 2014.
  • Yuan K et al. Illuminating DNA replication during Drosophila development using TALE-lights. Current Biol 2014.
  • Martinez-Llordella M et al. CD28-inducible transcription factor DEC1 is required for efficient autoreactive CD4+ T cell response. JEM 2013.
  • Chen C et al. Integrin a9B1 in airway smooth muscle suppresses exaggerated airway narrowing. JCI 2012.
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Our Services (23)


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Genomic DNA Extraction

Starting at $641.82 per cell line

We can extract genomic DNA from ES cells on 96-well plate with minimal cost for your own Southern or PCR genotyping.

We can extract genomic DNA from ES cells on 96-well plate with minimal cost for your own Southern or PCR genotyping.

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TALEN Design and Validation

Starting at $6,202.87 per prep

Service available only to UCSF researchers. Tell us where you would like to make double-strand breaks in the genome. We design and make TALEN expression plasmids that you can use for either “knockout” (by non-homologous end joining mechanism) or “knock-in” (by homologous recombination mechanism with a donor vector/oligos)... Show more »

Service available only to UCSF researchers. Tell us where you would like to make double-strand breaks in the genome. We design and make TALEN expression plasmids that you can use for either “knockout” (by non-homologous end joining mechanism) or “knock-in” (by homologous recombination mechanism with a donor vector/oligos) approaches to edit the genome. TALENs can be applied to cell lines and embryos of fly, worm, fish, and mammals.

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Southern Blot

Price on request
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Cell Line Generation

Price on request
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Karyotyping

Starting at $857.41 per clone
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CRISPR/Cas9 Genome Editing

Price on request
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Embryonic Stem Cell (ESC) Culture

Starting at $1,037.03 per clone

Investigators provide one vial of frozen ES cells with information about culture condition from original resource. We will revive, nurture, and refreeze ES cells (5 vials) when they are ready. In addition, we will give you 1~2 million cells for your genotyping verification.

Investigators provide one vial of frozen ES cells with information about culture condition from original resource. We will revive, nurture, and refreeze ES cells (5 vials) when they are ready. In addition, we will give you 1~2 million cells for your genotyping verification.

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ES Cell Targeting

Starting at $5,578.83 per cell line

Investigator’s targeting construct will be electroporated by core personnel. We have two feeder-independent ES cell lines, E14 (129-derived) and JM8A3.N1 (C57BL/6-derived) you can choose from. After drug selection for about one week, up to 300 colonies will be picked. When they are about to be confluent, we will split them as... Show more »

Investigator’s targeting construct will be electroporated by core personnel. We have two feeder-independent ES cell lines, E14 (129-derived) and JM8A3.N1 (C57BL/6-derived) you can choose from. After drug selection for about one week, up to 300 colonies will be picked. When they are about to be confluent, we will split them as duplicate, one master plate to freeze for future expansion of positive clones and one plate for genotyping to identify targeted ES cell clones. Your plates for genotyping will be ready for pick-up 2-3 weeks after the electroporation date.

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CRISPR Cloning

Starting at $1,463.45 per prep

Tell us where you would like to make a double-strand cut for knockout, knockin, or a larger genomic deletion. We will design and subclone sgRNAs to modified PX330/PX335 and sequence-verify them. We make 3~4 gRNAs per target site.

Tell us where you would like to make a double-strand cut for knockout, knockin, or a larger genomic deletion. We will design and subclone sgRNAs to modified PX330/PX335 and sequence-verify them. We make 3~4 gRNAs per target site.

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Genetic Engineering

Price on request
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DNA Extraction and Purification

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DNA Services

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Clinical Laboratory Services

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Clinical Research

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Nucleic Acid Services

Price on request
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Biochemistry & Molecular Biology

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Cells and Tissues

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Cell and Tissue Culture

Price on request
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DNA Fragment Analysis

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Chromosome Analysis

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Biology

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Mammalian Cell Culture

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Clinical Molecular Diagnostics

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Jennifer Page

Manager

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