Speed and Quality:
End-to-end chromatin immunoprecipitation sequencing includes all of the following:
Chromatin Isolation and Fragmentation, Chromatin Immunoprecipitation, Library Preparation, qPCR Quantification of Library, Agilent Bioanalyzer Library Analysis QC, Illumina Sequencing (single read 50 nts), Bioinformatics Data Analysis
Prices negotiable for qualifying institutions, order size, as well as price matching.
End-to-end DNA methylation sequencing services includes DNA isolation, bisulfite treatment, library preparation, qPCR quantification of library, Agilent Bioanalyzer QC, Illumina Sequencing Single Read 50 nts, bioinformatics data analysis. The following options are:
Bisulfite sequencing method to detect 3-4 million unique CpG sites, covering nearly all CpG islands and gene promoters.
Bisulfite sequencing method to detect 7-8 million unique CpG sites, covering nearly all CpG islands, gene promoters , and most genetic regulatory elements, gene bodies, and repeated DNA sequences.
Bisulfite sequencing method to detect in-depth DNA methylation across the entire genome, including methylation at CpG sites and less common non-CpG site such as CNG.
Antibody capture method to identify methylated sequences on a whole genome basis. End-to-end service comprehensively includes DNA isolation, fragmentation, library prep and QC, sequencing, and bioinformatics.
End-to-end DNA hydroxymethylation sequencing services include hydroxymethylated DNA enrichment, library preparation, qPCR quantification of library, Agilent Bioanalyzer QC, Illumina Sequencing Single Read 50 nts, bioinformatics data analysis.
Methylation-specific PCR approach to detect and identify methylated cytosines at single base resolution within one or several gene targets.
Utilizes MethylFlash(TM) method to deliver absolute quantification data of known amounts of methylated (5mC), hydroxymethylated (5hmC), or formylcytosine (5fC) DNA in your samples at a more cost effective price than HPLC, mass spec, or other chromatography based methods.
Screen histone samples for amount of post-translationally modified histones, including methylated, acetylated, and phosphorylated modifications at specific sites/patterns.
Add histone extraction from cells/tissues: +$25 per sample
Investigate DNA-protein interactions with a provided ChIP-grade antibody of interest. End-to-end service comprehensively includes chromatin isolation, fragmentation, antibody validation, primer design and synthesis, chromatin immunoprecipitation, qPCR, and data reporting.
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