EpigenDx

EpigenDx perform Affymetrix CytoScan array for the research communities

Targeted NextGen Bisulfite Sequencing: EpigenDx uses Ion Torrent for targeted NextGen bisulfite sequencing. The custom targets are ranged from 10 to more than 1000 CpG loci. The base-pair resolution and validation is provided. Each analysis includes up to 20 PCR amplicons at a time.

Pyrosequencing quantitative genotyping and allele quantification analysis

Human Global Methylation Analysis Assays:
Human Line-1 Methylation Analysis: Long interspersed elements make up about 15% of human genome and contain much of the CpG methylation found in normal postnatal somatic tissues.

Human Alu Repetitive Elements: Alu repeat is the most plentiful short interspersed nucleotide element in human DNA. It comprises 10% of the human genome.

Mouse Global Methylation Analysis Assays:
Mouse B1 Repetitive Element: There are 30,000 copies of the B1 element are randomly dispersed in the total mouse genome.

Mouse Line-1 Methylation Analysis: Long interspersed elements make up about 15% of mouse genome and contain much of the CpG methylation found in normal postnatal somatic tissues...

Rat Global Methylation Analysis Assays:
Rat ID Element Methylation Analysis:
Rat Line-1 Methylation Analysis: Long interspersed elements make up about 15% of rat genome and contain much of the CpG methylation found in normal postnatal somatic tissues...

PCR from genomic DNA for Pyrosequencing allele quantification analysis

Design and optimize PCR and Pyrosequencing primers for DNA methylation analysis, SNP genotyping, and allele specific expression analysis:

Design PCR and Pyrosequencing primers
Develop and optimize PCR condition
Develop and optimize Pyrosequencing condition
Perform assay validation

Customers will submit the DNA sequence containing the target region of interest. We deliver an assay design report with recommendations for PCR and Pyrosequencing primers. Customer will run PCR and Pyrosequencing based on our design.
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Customers submit the DNA sequence containing the target region of interest. We deliver an assay design report including PCR and Pyrosequencing primers. Customers will run PCR based on our design and send the PCR product to us for Pyrosequencing analysis. Inquiry / or
Customers submit the DNA sequence containing target region of interest. We optimize the PCR and Pyrosequencing assay on 10 DNA samples or controls and deliver an assay design report with PCR and Pyrosequencing primers and assay conditions along with performance data.

Perform PCR

Perform Pyrosequencing genotyping and allele quantification analysis