EpigenDx
9.6/10 Overall Rating
100% Positive
32 Orders Completed
Hopkinton, Massachusetts, US
EpigenDx is a genomic and epigenomic research company specializing in disease biomarker discovery and molecular diagnosis.
The company provides products related to DNA methylation analysis research. Currently available products include DNA methylation controls and validated DNA methylation assays for human, mouse, and rat.
EpigenDx also provides products and laboratory services for scientific researchers from academic, government and industrial communities. Our commitment to quality comes from our desire and dedication to provide the best products and services to our customers. EpigenDx has knowledge and expertise in Pyrosequencing and its many applications.
CpG methylation and allele quantification analysis are conducted using Qiagen-Pyrosequencing PSQ MD system, while short-read sequence analysis is carried out using Qiagen-Pyrosequencing PSQ ID system.
EpigenDx perform Affymetrix CytoScan array for the research communities
Targeted NextGen Bisulfite Sequencing: EpigenDx uses Ion Torrent for targeted NextGen bisulfite sequencing. The custom targets are ranged from 10 to more than 1000 CpG loci. The base-pair resolution and validation is provided. Each analysis includes up to 20 PCR amplicons at a time.
Pyrosequencing quantitative genotyping and allele quantification analysis
Human Global Methylation Analysis Assays:
Human Line-1 Methylation Analysis: Long interspersed elements make up about 15% of human genome and contain much of the CpG methylation found in normal postnatal somatic tissues.
Human Alu Repetitive Elements: Alu repeat is the most plentiful short interspersed nucleotide element in human DNA. It comprises 10% of the human genome.
Mouse Global Methylation Analysis Assays:
Mouse B1 Repetitive Element: There are 30,000 copies of the B1 element are randomly dispersed in the total mouse genome.
Mouse Line-1 Methylation Analysis: Long interspersed elements make up about 15% of mouse genome and contain much of the CpG methylation found in normal postnatal somatic tissues...
Rat Global Methylation Analysis Assays:
Rat ID Element Methylation Analysis:
Rat Line-1 Methylation Analysis: Long interspersed elements make up about 15% of rat genome and contain much of the CpG methylation found in normal postnatal somatic tissues...
PCR from genomic DNA for Pyrosequencing allele quantification analysis
Design and optimize PCR and Pyrosequencing primers for DNA methylation analysis, SNP genotyping, and allele specific expression analysis:
Design PCR and Pyrosequencing primers
Develop and optimize PCR condition
Develop and optimize Pyrosequencing condition
Perform assay validation
Customers will submit the DNA sequence containing the target region of interest. We deliver an assay design report with recommendations for PCR and Pyrosequencing primers. Customer will run PCR and Pyrosequencing based on our design.
or
Customers submit the DNA sequence containing the target region of interest. We deliver an assay design report including PCR and Pyrosequencing primers. Customers will run PCR based on our design and send the PCR product to us for Pyrosequencing analysis. Inquiry / or
Customers submit the DNA sequence containing target region of interest. We optimize the PCR and Pyrosequencing assay on 10 DNA samples or controls and deliver an assay design report with PCR and Pyrosequencing primers and assay conditions along with performance data.
Perform PCR
Perform Pyrosequencing genotyping and allele quantification analysis
"EpigenDx provided high quality service. They gave me information about the pyrosequencing assays that were relevant to my gene of interest and helped me to choose the most appropriate assay. They were also thorough in testing the quality of my samples before doing the assay. EpigenDx was professional and timely in performing pyrosequencing for my project, and working with EpigenDx was a very positive experience."
"Excellent job."
"Yes, it is very efficient. Good Job!"
"It is very efficient, having quick turn-around time. Quality is very good."
"Great, fast, and professional genotyping service. I would highly recommend them."
"I have a marvelous experience in collaborating with this lab. Everything, including the price quotes, the quality of experimentation and results, the time schedule, was fantastic. I am highly impressed by their services and would be very happy to collaborate in my future projects also! Thanks to the entire Science Exchange team especially Ms Sarah Miller, Ms Liying, and Ms. Melissa"
EpigenDx has not received any endorsements.