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Enzymax, LLC.

3 Orders Completed
Lexington, Kentucky, US

About Enzymax, LLC.

Enzymax, LLC, was formed in August of 2002 as a Biotech company in Lexington, Kentucky. The Enzymax laboratory started on the University of Kentucky campus in the ASTeCC building (Advanced Science and Technology Commercialization Center). In October of 2006, the Enzymax laboratory moved to its present location with much larger space and better equipment. Our goal is to maximize your research productivity by providing various molecular and biochemical services, by providing purified tool enzymes and proteins, and by providing a wide variety of purified specialty enzymes and proteins for your biological/biomedical research needs. In today's highly competitive research environment, top quality and high efficiency are two important factors contributing to one's competitive advantage. Enzymax is your quality and efficiency facilitator.

Our Services (27)


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Animal Immunization for Antibody Production

Price on request
Request a quote for more information about this service.
Rabbit Mouse Rat Chicken Pig Guinea pig Goat Show 7 more tags Show less

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Bacterial Protein Expression

Starting at $1,900.00 per prep

Custom Protein Purification in Bacterial Expression System;
Step 1: Gene synthesis with codon optimization, sequencing confirmation, protein express checking in small scale Step2: large scale protein production from 1 liter culture

We can purify your protein from the following systems. His Tag (or GST tag) can be removed nearly ~99% with our ULP1 Sumo Protease
(a). E. coli expression system ($1,900).
(b). Baculovirus system


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Protein Expression in Yeast

Starting at $3,200.00 per prep

Custom Protein Purification in Yeast Expression System (for both Pichia pastoris and Saccharomyces cerevisiae):
Step 1: Gene synthesis with codon optimization, Sequencing confirmation, checking protein expression in small scale. Step 2: Large scale protein expression from 1-3 liters yeast culture


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Antibody mRNA Sequencing

Starting at $900.00 per sample

Description:
Monoclonal Antibody Sequencing or Mab Sequencing Services are provided by Enzymax. You just need to send us the pellet of snap frozen cells (1x107) from any antibody producing cell line (hybridoma cell lines or Clonal B-cell line). We will send the sequencing data of variable regions of heavy chain (VH), light chain (VL), constant domains, and leader sequence (signal sequence). Here is our standard sequencing procedures:
• Isolate mRNA from hybidoma or B cells
• cDNA synthesis by reverse transcription: mRNA to cDNA
• 5’ RACE amplification of the constant regions of Heavy (CH) and Light Chain (CL) for leader sequence sequencing
• PCR amplification of DNA fragments corresponding to VH and VL
• Cloning into a standard sequencing vector
• Sequencing analysis from at least 5-10 independent clones for each of VH and VL
• Report of sequencing data or provide plasmid containing coding sequence (by request)

Three services available:

  1. Variable Domains Sequencing for both Light chain and Heavy chain:
    Cat# CS8-1: $900, 7-10 business day

  2. Variable Domains Sequencing for both LV and HV, plus leading sequence:
    Cat# CS8-2: $1400, 10-12 business days

  3. Full Length sequencing for Variable regions, constant domains and leader sequences:
    Cat# CS8-3: $2200, 12-14 business days


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Hybridoma Sequencing

Starting at $900.00 per cell line

Description:
Monoclonal Antibody Sequencing or Mab Sequencing Services are provided by Enzymax. You just need to send us the pellet of snap frozen cells (1x107) from any antibody producing cell line (hybridoma cell lines or Clonal B-cell line). We will send the sequencing data of variable regions of heavy chain (VH), light chain (VL), constant domains, and leader sequence (signal sequence). Here is our standard sequencing procedures:
• Isolate mRNA from hybidoma or B cells
• cDNA synthesis by reverse transcription: mRNA to cDNA
• 5’ RACE amplification of the constant regions of Heavy (CH) and Light Chain (CL) for leader sequence sequencing
• PCR amplification of DNA fragments corresponding to VH and VL
• Cloning into a standard sequencing vector
• Sequencing analysis from at least 5-10 independent clones for each of VH and VL
• Report of sequencing data or provide plasmid containing coding sequence (by request)

Three services available:

  1. Variable Domains Sequencing for both Light chain and Heavy chain:
    Cat# CS8-1: $900, 7-10 business day

  2. Variable Domains Sequencing for both LV and HV, plus leading sequence:
    Cat# CS8-2: $1400, 10-12 business days

  3. Full Length sequencing for Variable regions, constant domains and leader sequences:
    Cat# CS8-3: $2200, 12-14 business days


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BAC DNA Extraction

Starting at $253.00 per sample

Enzymax offers high quality custom nucleic acid and plasmid DNA, BAC DNA purification services at a
competitive price. Among the services we provide, plasmid DNA purification is the most popular one
benefited from our proprietary DNA purification protocols. Other than the purification of plasmids for
routine molecular biology research uses, we provide our customers with the highest quality plasmid DNA
that are "Transfection Grade" for mammalian cells, or "Ready-to-Inject" for transgenic applications.
Furthermore, we also provide other services based on the specific needs of our customers. Please contact us
for your special service or quotation.


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Elisa Kit Development

Starting at $18,000.00 per sample

Monoclonal - Polyclonal Sandwich Assay Development
Required materials and reagents to begin assay development:

  1. Analyte.
  2. Antibodies.
  3. Analyte negative samples.
  4. Analyte positive samples.
    Step I: Reagent Preparation and Initial Assay Titration
    Step II: Assay Optimization
    Step III: Assay Validation
    Step IV: Production of 25 Finished Kits


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Molecular Cloning

Starting at $450.00 per sample

You provide us with a gene name, or gene sequence, or an open reading frame (ORF) name, we will clone the gene into our vector or your vector. We can build your gene into various vectors for: (a) RNA interference (RNAi); (b) tissue-specific gene expression; (c) bacterial, yeast, mammalian, and baculovirus expression; (d) reporter gene studies (including drug resistance, green, red, blue, yellow fluorescence, etc); and (e) targeted gene knock-out.


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Plasmid DNA Preparation

Starting at $89.00 per sample

Enzymax offers high quality custom nucleic acid and plasmid DNA, BAC DNA purification services at a competitive price. Among the services we provide, plasmid DNA purification is the most popular one benefited from our proprietary DNA purification protocols. Other than the purification of plasmids for routine molecular biology research uses, we provide our customers with the highest quality plasmid DNA that are "Transfection Grade" for mammalian cells, or "Ready-to-Inject" for transgenic applications. Furthermore, we also provide other services based on the specific needs of our customers. Please contact us for your special service or quotation.


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Polyclonal Antibody Production

Starting at $599.00 per sample

We offer custom polyclonal antibody production in Rat, Guinea pig, rabbit, chicken, mouse, and goat. ELISA results of 1:50,000 for any peptide polyclonal antibody project. No cross-reactivity for phosphopeptide-specific polyclonal projects, and a guaranteed 1:10,000 ELISA titer
(a) Custom antibody production from your antigen ($599):
We use your antigen (about 1 mg of protein for each rabbit). This service includes 2 rabbits and ELISA.
(b) Custom peptide antibody production ($899):
We design and synthesize peptide antigen (15 amino acids, 95% purity) for you. This service also includes 2 rabbits and ELISA.
(c) Custom phosphor-peptide antibody production ($2120):
The Service includes phosphor and non-phosphor peptide synthesis, peptide-KLH conjugation, two rabbit immunizations with 70 days standard protocol, and antisera purification with two steps phosphor-peptide affinity purification. The price of without antisera purification is $1,120.
(d) Custom fusion protein purification and antibody production ($2400):
We will choose a coding region (~300 amino acids), which is the most antigenic and divergent, as the antigen, and clone the selected region into an E. coli expression vector. We will purify about 2 mg fusion protein to inject two rabbits for antibody production. We will send the full-length cDNA to you if available. This service includes 2 rabbits and ELISA.

Rat Mouse Rabbit Guinea pig Pig Chicken Goat Show 7 more tags Show less

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Monoclonal Antibody (mAb) Production

Starting at $3,999.00 per sample

The service includes 5 balb/c mice immunization, fusions, clone screen, subclone culture, stabilization and expansion.
ELISA titers of at least 1:20,000 for all peptide monoclonal antibody projects
1-3 positive clones for all monoclonal antibody projects

10mg of purified protein will be provided by customers. The ideal concentration will be 1 mg/ml within PBS buffer or other physical buffers.
If using peptide as antigen, there will be $600 additional cost for peptide synthesis and KLH conjugation.


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Protein Services

Price on request
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RNA Services

Price on request
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Plasmids and Vectors

Price on request
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Protein Quantification

Price on request
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Antibody Services

Price on request
Request a quote for more information about this service.

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DNA Extraction and Purification

Price on request
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Nucleic Acid Services

Price on request
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DNA Synthesis and Probe Development

Price on request
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Bacterial Plasmid Vector

Price on request
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Yeast Artificial Chromosome (YAC)

Price on request
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Therapeutic Monoclonal Antibody (mAb) Development

Price on request
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Biology

Price on request
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Biochemistry & Molecular Biology

Price on request
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DNA Sequencing

Price on request
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Protein Production

Price on request
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DNA Services

Price on request
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Michelle Wu

LLC Member

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