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Electron Microscopy Core Facility

Omaha, Nebraska, US

The University of Nebraska Medical Center’s Electron Microscopy Core Facility (EMCF) provides instrumentation and technical assistance to research programs utilizing Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM). The EMCF is available to all University of Nebraska personnel, as well as outside institutions and industrial users. The Director and two full-time Electron Microscopy Specialists are available for consultation and service. The managing microscopist has 40 years of experience and is fully certified by the Microscopy Society of America (MSA). His expertise cover a diverse range of specimens including bacteria, viruses, algae, marine organisms, particulate samples, as well as eukaryotic cell structure and tissue architecture. The facility is supported, in part, by user fees to cover supplies, instrument time and the EM Specialists' time to carry out any part of a... Show more »

The University of Nebraska Medical Center’s Electron Microscopy Core Facility (EMCF) provides instrumentation and technical assistance to research programs utilizing Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM). The EMCF is available to all University of Nebraska personnel, as well as outside institutions and industrial users. The Director and two full-time Electron Microscopy Specialists are available for consultation and service. The managing microscopist has 40 years of experience and is fully certified by the Microscopy Society of America (MSA). His expertise cover a diverse range of specimens including bacteria, viruses, algae, marine organisms, particulate samples, as well as eukaryotic cell structure and tissue architecture. The facility is supported, in part, by user fees to cover supplies, instrument time and the EM Specialists' time to carry out any part of a project.

Visit our website at: http://www.unmc.edu/vcr/cores/vcr-cores/electron-microscopy/index.html

Featured Publications

  • Dutta, S., Roy, S., Polavaram, N. S., Stanton, M. J., Zhang, H., Bhola, T., Honscheid, P., Donohue, T. M., Band, H., Batra, S. K., Muders, M. H., Datta, K. (2016). Neuropilin-2 regulates endosome maturation and EGFR trafficking to support cancer cell pathobiology. Cancer Research, 76(2), 418.
    University of Nebraska Medical Center

  • Ericson, B. L., Carlson, D. J., & Carlson, K. A.* (2016). Characterization of nora virus structural proteins via western blot analysis. Scientifica, 2016.
    University of Nebraska Kearney

  • Wu, B., Huang, Y., Braun, A. L., Tong, Z., Zhao, R., Li, Y., Liu, F., Zheng, J. C. (2015). Glutaminase-containing microvesicles from HIV-1-infected macrophages and immune-activated microglia induce neurotoxicity. Molecular Neurodegeneration, 10(1), 61.
    University of Nebraska Medical Center

  • Ogun, O., & Zallocchi, M. (2014). Clarin-1 acts as a modulator of mechanotransduction activity and presynaptic ribbon assembly. The Journal of Cell Biology, 207(3), 375.
    Boystown Research Hospital

  • Romereim, S. M., Conoan, N. H., Baojiang, C., Dudley, A. T. (2014). A dynamic cell adhesion surface regulates tissue architecture in growth plate cartilage. Development, 141(10).
    University of Nebraska Medical Center

  • Mistry, P. H., Mohapatra, S. K., & Dash, A. K. (2012). Effect of high-pressure homogenization and stabilizers on the physicochemical properties of curcumin-loaded glycerol monooleate/chitosan nanostructures. Nanomedicine (London, England), 7(12), 1863.
    Creighton University

  • Thurlow, L. R., Hanke, M. L., Fritz, T., Angle, A., Aldrich, A., Williams, S. H., Engebretsen, I. L., Bayles, K. W., Horswill, A. R., Kielian, T. (2011). Staphylococcus aureus biofilms prevent macrophage phagocytosis and attenuate inflammation in vivo. Journal of Immunology (Baltimore, Md.: 1950), 186(11), 6585.
    University of Nebraska Medical Center

  • Kim, J. O., Sahay, G., Kabanov, A. V., & Bronich, T. K. (2010). Polymeric micelles with ionic cores containing biodegradable cross-links for delivery of chemotherapeutic agents. Biomacromolecules, 11(4), 919.
    University of Nebraska Medical Center

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Transmission Electron Microscopy (TEM)
Transmission Electron Microscopy services
Price on request

-Tissue processing and embedding
-Ultramicrotomy
-Standard analysis of thin sections
-Negative staining of particulate samples
-Quantitative analysis using stereology
-Array tomography
-Immunocytochemistry using immunogold antibodies

-Tissue processing and embedding
-Ultramicrotomy
-Standard analysis of thin sections
-Negative staining of particulate samples
-Quantitative analysis using stereology
-Array tomography
-Immunocytochemistry using immunogold antibodies

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Scanning Electron Microscopy (SEM)
Scanning Electron Microscopy services
Price on request

-Specimen preparation
-Critical point drying
-Sputter coating
-Elemental Analysis using EDS
-Environmental SEM for non-conductive specimens

-Specimen preparation
-Critical point drying
-Sputter coating
-Elemental Analysis using EDS
-Environmental SEM for non-conductive specimens

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Imaging & Spectroscopy
Price on request
Request a quote for more information about this service.
Microscopy
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Electron Microscopy
Price on request
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2018-07-05 09:05:55 -0500

Net Promoter Score of 10 received for Transmission Electron Microscopy (TEM).

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2018-06-19 08:48:05 -0500

Net Promoter Score of 10 received for Transmission Electron Microscopy (TEM).

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2018-04-26 14:43:19 -0500

Net Promoter Score of 10 received for Transmission Electron Microscopy (TEM).

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
February 5, 2018

Positive review received for Transmission Electron Microscopy (TEM):

"Working with the Electron Microscopy Core Facility was a great experience. Nick, who I worked with, was very knowledgeable, efficient and thorough. I was extremely happy with the turn around time, as Nick provided all the images requested within a couple days of receiving the samples. I plan to go back to the UNMC Electron Microscopy Core Facility again for any future TEM needs."

2018-02-02 12:09:38 -0600

Net Promoter Score of 10 received for Transmission Electron Microscopy (TEM).

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2018-02-01 14:10:47 -0600

Net Promoter Score of 10 received for Transmission Electron Microscopy (TEM).

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.

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