COVID-19 Update 3-26-2020: We would like to inform requestors that our lab will remain open until further notice. We currently do not have any shipping restrictions so we will continue to offer our services as normal. Thank you for your continued support of our facility!
The University of Nebraska Medical Center’s Electron Microscopy Core Facility (EMCF) provides instrumentation and technical assistance to research programs utilizing Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM). The EMCF is available to all University of Nebraska personnel, as well as outside institutions and industrial users. The Director and two full-time Electron Microscopy Specialists are available for consultation and service. The managing microscopist has 40 years of experience and is fully certified by the Microscopy Society of America (MSA). His expertise cover a diverse range of specimens including bacteria, viruses, algae, marine organisms, particulate samples, as well as eukaryotic cell structure and tissue architecture. The facility is supported, in part, by user fees to cover supplies, instrument time and the EM Specialists' time to carry out any part of a project. Visit our website at: http://www.unmc.edu/vcr/cores/vcr-cores/electron-microscopy/index.html **Featured Publications** - Dutta, S., Roy, S., Polavaram, N. S., Stanton, M. J., Zhang, H., Bhola, T., Honscheid, P., Donohue, T. M., Band, H., Batra, S. K., Muders, M. H., Datta, K. (2016). Neuropilin-2 regulates endosome maturation and EGFR trafficking to support cancer cell pathobiology. Cancer Research, 76(2), 418. **University of Nebraska Medical Center** - Ericson, B. L., Carlson, D. J., & Carlson, K. A.* (2016). Characterization of nora virus structural proteins via western blot analysis. Scientifica, 2016. **University of Nebraska Kearney** - Wu, B., Huang, Y., Braun, A. L., Tong, Z., Zhao, R., Li, Y., Liu, F., Zheng, J. C. (2015). Glutaminase-containing microvesicles from HIV-1-infected macrophages and immune-activated microglia induce neurotoxicity. Molecular Neurodegeneration, 10(1), 61. **University of Nebraska Medical Center** - Ogun, O., & Zallocchi, M. (2014). Clarin-1 acts as a modulator of mechanotransduction activity and presynaptic ribbon assembly. The Journal of Cell Biology, 207(3), 375. **Boystown Research Hospital** - Romereim, S. M., Conoan, N. H., Baojiang, C., Dudley, A. T. (2014). A dynamic cell adhesion surface regulates tissue architecture in growth plate cartilage. Development, 141(10). **University of Nebraska Medical Center** - Mistry, P. H., Mohapatra, S. K., & Dash, A. K. (2012). Effect of high-pressure homogenization and stabilizers on the physicochemical properties of curcumin-loaded glycerol monooleate/chitosan nanostructures. Nanomedicine (London, England), 7(12), 1863. **Creighton University** - Thurlow, L. R., Hanke, M. L., Fritz, T., Angle, A., Aldrich, A., Williams, S. H., Engebretsen, I. L., Bayles, K. W., Horswill, A. R., Kielian, T. (2011). Staphylococcus aureus biofilms prevent macrophage phagocytosis and attenuate inflammation in vivo. Journal of Immunology (Baltimore, Md.: 1950), 186(11), 6585. **University of Nebraska Medical Center** - Kim, J. O., Sahay, G., Kabanov, A. V., & Bronich, T. K. (2010). Polymeric micelles with ionic cores containing biodegradable cross-links for delivery of chemotherapeutic agents. Biomacromolecules, 11(4), 919. **University of Nebraska Medical Center**
-Tissue processing and embedding
-Standard analysis of thin sections
-Negative staining of particulate samples
-Quantitative analysis using stereology
-Immunocytochemistry using immunogold antibodies
-Critical point drying
-Elemental Analysis using EDS
-Environmental SEM for non-conductive specimens
"Working with the Electron Microscopy Core Facility was a great experience. Nick, who I worked with, was very knowledgeable, efficient and thorough. I was extremely happy with the turn around time, as Nick provided all the images requested within a couple days of receiving the samples. I plan to go back to the UNMC Electron Microscopy Core Facility again for any future TEM needs."
Electron Microscopy Core Facility has not received any endorsements.