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Ecrins Therapeutics Services

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La Tronche, FR

About Ecrins Therapeutics Services

Welcome to Ecrins Therapeutics Services, a biotech offering unique services in drug discovery. We are specialist of the cytoskeleton (tubulin, MAPs…) and we have a strong expertise in cellular biology and phenotypic characterization.

Working on cytoskeleton or his effectors? You need precise, reliable, and... Show more »

Welcome to Ecrins Therapeutics Services, a biotech offering unique services in drug discovery. We are specialist of the cytoskeleton (tubulin, MAPs…) and we have a strong expertise in cellular biology and phenotypic characterization.

Working on cytoskeleton or his effectors? You need precise, reliable, and innovative assay to characterize the effect of your molecules on microtubules?
With more than 20 years of expertise in cytoskeleton, we have solutions for you.

You have molecules? You need to characterize their biological effect? You need a tailored assay to evaluate your molecules?
Our scientists developed dozens of specific test to evaluate small molecules ( Protein expression (cells, tissus…) vs molecule activity, Phenotypic characterization, Protein-Protein Interaction studies, Off-target/On-target studies, angiogenesis assay...)

We can develop the assay that you need with clear go/no-go and then test hundreds of compounds in weekly basis. Save time and build directly your SAR in living cells.

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Our Services (19)


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Native Tubulin

Price on request

Highly pure (>99%) bovine and porcine brain tubulin.
Tubulin is isolated using a proprietary protocol to ensure the efficient removal of contaminants. We supply a highly concentrated and polymerization-competent tubulin solution for all types of in vitro assays.

Stable for 12 months after delivery when stored at < -80°C or in... Show more »

Highly pure (>99%) bovine and porcine brain tubulin.
Tubulin is isolated using a proprietary protocol to ensure the efficient removal of contaminants. We supply a highly concentrated and polymerization-competent tubulin solution for all types of in vitro assays.

Stable for 12 months after delivery when stored at < -80°C or in liquid nitrogen. Thawing and refreezing may cause partial tubulin incativation/aggregation.

5 mg, 10 mg, 100 mg, Bulk amount on demand

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Tubulin Polymerization Assay Kits

Starting at $515.00 per unit

Bovine or porcine brain tubulin kits contain all components necessary to perform in vitro tubulin polymerization assay. We provide a high purity polymerization-competent tubulin, as well as two control molecules: taxol, a microtubule-stabilizing molecule, and colchicine, a tubulin depolymerizing compound.

Each kit contains... Show more »

Bovine or porcine brain tubulin kits contain all components necessary to perform in vitro tubulin polymerization assay. We provide a high purity polymerization-competent tubulin, as well as two control molecules: taxol, a microtubule-stabilizing molecule, and colchicine, a tubulin depolymerizing compound.

Each kit contains enough reagents for at least 16 measurements:

  • 2 x 130 µL 240 µM bovine or porcine tubulin stock in 1x PB-GTP
  • 300 µL 5x Polymerization Buffer (PB)
  • 20 µL 200 mM GTP
  • Half-area flat bottom 96-well plate
  • 10 µL 700 µM taxol in DMSO
  • 10 µL 700 µM colchicine in DMSO
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Tubulin Polymerization Assays

Price on request

You have identified a new bioactive molecule and wish to make sure it affect tubulin polymerization or, on the contrary, to exclude thaht your compound interferes with tubulin.

Tubulin polymerization is followed spectrophotometrically over one hour in the presence of your compounds. Measurements are performed in triplicate in... Show more »

You have identified a new bioactive molecule and wish to make sure it affect tubulin polymerization or, on the contrary, to exclude thaht your compound interferes with tubulin.

Tubulin polymerization is followed spectrophotometrically over one hour in the presence of your compounds. Measurements are performed in triplicate in parallel with control compounds. For microtubule depolymerization agents, the activity is presented in the form of IC50.

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In vitro Angiogenesis Assays

Price on request

Compounds are tested for their ability to either:

  • prevent in vitro endothelial cell tube formation (anti-angiogenic potency)
  • disrupt in vitro preformed capillary tubes (vascular disruption activity, VDA)

Our assay includes standard references: Sunitinib (anti-angiogenic compound, VEGFR inhibitor) and Combretastatin 4-phosphate (CA4-P, a tubulin-binding vascular disrupting agent).

Compounds are tested for their ability to either:

  • prevent in vitro endothelial cell tube formation (anti-angiogenic potency)
  • disrupt in vitro preformed capillary tubes (vascular disruption activity, VDA)

Our assay includes standard references: Sunitinib (anti-angiogenic compound, VEGFR inhibitor) and Combretastatin 4-phosphate (CA4-P, a tubulin-binding vascular disrupting agent).

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Immunofluorescence

Price on request

Cytoskeleton staining assay

The cell-based Immunofluorescence assay allows evaluation of the morphology of the microtubule and actin cytoskeleton in drug-treated cells.
This assay allows the identification of compounds which:

  • interfere with the microtubule/actin cytoskeleton
  • interfere with cell proliferation and/or... Show more »

Cytoskeleton staining assay

The cell-based Immunofluorescence assay allows evaluation of the morphology of the microtubule and actin cytoskeleton in drug-treated cells.
This assay allows the identification of compounds which:

  • interfere with the microtubule/actin cytoskeleton
  • interfere with cell proliferation and/or arrest cells in mitosis

Cells are incubated in the presence of test compounds, followed by fixation, labelling, and indirect immunofluorescence microscopy to reveal microtubules, actin or DNA.

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Cell-Based Assays

Price on request

Benefit of our large cell panel (cancer and non-cancer cells) to characterize the effect of your molecules before going in vivo. Ask us for a custom protocol and identify correlation between protein expression and efficacy of your molecule.
Cell viability is typically assessed by the “MTT assay” which is based on the ability of... Show more »

Benefit of our large cell panel (cancer and non-cancer cells) to characterize the effect of your molecules before going in vivo. Ask us for a custom protocol and identify correlation between protein expression and efficacy of your molecule.
Cell viability is typically assessed by the “MTT assay” which is based on the ability of living cells to reduce the tetrazolium dye MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide to its insoluble and colored form formazan. The results can be presented in the form of GI50, (Growth Inhibition 50%), showing the drug concentration that reduces cell proliferation by 50%.
Additionally, we will provide you with phenotypic data such as the presence of dead cells, mitotic arrest and much more. In this way, we can accurately determine the cellular effects of your compounds and classify them according to phenotype.
We also use other tests to evaluate the effect of drugs on cell viability, just ask!

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Small Molecule Bioanalysis

Price on request

Our scientists developed dozens of specific test to evaluate small molecules.
We can develop the assay that you need with clear go/no-go and then test hundreds of compounds in weekly basis. Save time and build directly your SAR in living cells.

  • Tailored test to evaluate your molecules
  • Build your SAR in living cells
    -... Show more »

Our scientists developed dozens of specific test to evaluate small molecules.
We can develop the assay that you need with clear go/no-go and then test hundreds of compounds in weekly basis. Save time and build directly your SAR in living cells.

  • Tailored test to evaluate your molecules
  • Build your SAR in living cells
  • Long-term commitment
  • Easy communication at every step of the way
  • A study director assigned to your project
  • CIR certified = 30% tax saving for you

Once you have selected your leads. Investigate in details their mechanism of action with us:

  • Protein expression (cells, tissus…) vs molecule activity
  • Phenotypic characterization
  • Protein-Protein Interaction studies
  • Off-target/On-target studies

Long-term following of your project: we can perform in vivo Proof-of-Concept with your leads (mice models).

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Drug Mechanism of Action Studies

Mechanism of Action Studies
Price on request

Once you have selected your leads. Investigate in details their mechanism of action with us:

  • Protein expression (cells, tissues…) vs molecule activity
  • Phenotypic characterization
  • Protein-Protein Interaction studies
  • Off-target/On-target studies

Once you have selected your leads. Investigate in details their mechanism of action with us:

  • Protein expression (cells, tissues…) vs molecule activity
  • Phenotypic characterization
  • Protein-Protein Interaction studies
  • Off-target/On-target studies
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Protein-Protein Interaction Analysis

Price on request
Request a quote for more information about this service.

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Assay Development

Price on request

Experimental Design Services

Experimental Design Services

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Angiogenesis & Endothelial Cell Assays

Price on request

Angiogenesis & Endothelial Cell Assays Services

Angiogenesis & Endothelial Cell Assays Services

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Biomolecular Interaction Analysis

Price on request

Biomolecular Interaction Analysis Services

Biomolecular Interaction Analysis Services

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Biochemistry & Molecular Biology

Price on request

Biochemistry & Molecular Biology Services

Biochemistry & Molecular Biology Services

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Medicinal Chemistry

Price on request

Medicinal Chemistry Services

Medicinal Chemistry Services

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Pharmacology & Toxicology

Price on request

Pharmacology & Toxicology Services

Pharmacology & Toxicology Services

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Biology

Price on request

Biology Services

Biology Services

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Protein Expression Visualization

Price on request

Protein Expression Visualization Services

Protein Expression Visualization Services

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Protein Purification

Price on request

Protein Purification and Quantification Services

Protein Purification and Quantification Services

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Biochemical Assays

Price on request

Biochemical Assays Services

Biochemical Assays Services

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