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Eclipse BioInnovations Inc.

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San Diego, California, US

About Eclipse BioInnovations Inc.

Eclipse BioInnovations Inc. is a RNA genomics company in San Diego, CA. Our founders are the leading experts in using RNA binding proteins to identify RNA genes and regulatory elements involved in many human diseases including neuromuscular disorders and cancer. By identifying these novel RNA targets, Eclipse... Show more »

Eclipse BioInnovations Inc. is a RNA genomics company in San Diego, CA. Our founders are the leading experts in using RNA binding proteins to identify RNA genes and regulatory elements involved in many human diseases including neuromuscular disorders and cancer. By identifying these novel RNA targets, Eclipse can help academic and industry researchers develop new and effective drugs to cure a wide range of human diseases.

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Our Services (2)


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eCLIP

Cross-linking immunoprecipitation
Price on request

Enhanced cross linking and immunoprecipitation followed by high-throughput sequencing (CLIP-seq).

Our eCLIP services are all-inclusive from lysis through library preparation, sequencing, and primary data processing.

Essential components of each eCLIP include:

Choice of sample type:

Enhanced cross linking and immunoprecipitation followed by high-throughput sequencing (CLIP-seq).

Our eCLIP services are all-inclusive from lysis through library preparation, sequencing, and primary data processing.

Essential components of each eCLIP include:

Choice of sample type:

  • Cancer cell line (HEK293T, K562, HepG2)
  • Stem cells (H1ES, human iPSC)
  • Differentiated stem cells (neurons, cardiomyocytes)
  • Tissues (brain, heart, liver)

Most biological samples are amenable to eCLIP, with optimization required depending on endogenous RNase levels and other biological properties. Please contact us if you have a particular sample type of interest not listed above.

Other custom samples can be used upon request, but may require UV crosslinking by the customer prior to shipment.

Choice of antibody:

  • Pre-validated antibodies
  • Other commercial or custom antibodies (requires validation service)
  • Tagged peptides (V5, FLAG)

Successful immunoprecipitation is critical for eCLIP success. We can provide pre-validation of untested antibodies, and can help customers to identify possible antibodies for a protein of interest.

Standard and customizable eCLIP services tailored to your experiment

Included in standard eCLIP experiments

  • Validation documents (for crosslinking, immunoprecipitation success, and library yield)
  • Raw sequencing data (.fastq files)
  • Primary analysis including mapped reads (.bam files) and identified peaks (.bed files)
  • Basic quality control report (including % of reads mapping, % PCR duplication, % of reads mapping to repetitive elements)
  • Basic analysis report (including meta-transcript binding profile, motif analysis (HOMER))
  • Our basic analysis includes everything necessary to identify peaks and perform basic analysis. Contact us for details about additional analysis options.

eCLIP experiment custom options:

  • Pilot experiments for large-scale studies
  • Number of biological or technical replicates (discounts available for large projects)
  • Additional sequencing depth available

Please contact us to discuss any other questions or options you are interested in!

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CLIP-Seq Data Analysis

Price on request

Included in standard eCLIP experiments:
- Validation documents (for crosslinking, immunoprecipitation success, and library yield)
- Raw sequencing data (.fastq files)
- Primary analysis including mapped reads (.bam files) and identified peaks (.bed files)
- Basic quality control report (including % of reads mapping, % PCR... Show more »

Included in standard eCLIP experiments:
- Validation documents (for crosslinking, immunoprecipitation success, and library yield)
- Raw sequencing data (.fastq files)
- Primary analysis including mapped reads (.bam files) and identified peaks (.bed files)
- Basic quality control report (including % of reads mapping, % PCR duplication, % of reads mapping to repetitive elements)
- Basic analysis report (including meta-transcript binding profile, motif analysis (HOMER))
- Our basic analysis includes everything necessary to identify peaks and perform basic analysis. Contact us for details about additional analysis options.

eCLIP experiment custom options:
- Pilot experiments for large-scale studies
- Number of biological or technical replicates (discounts available for large projects)
- Additional sequencing depth available
- Please contact us to discuss any other questions or options you are interested in!

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