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e-Zyvec

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About e-Zyvec

**Special prioritization available for COVID-19-related research. Click 'Request More Info' button on this page to ask about offer details.**

e-Zyvec is a biotech start-up specialized in genetic engineering. We provide biologists and genetic researchers with sophisticated tailor-made vectors. Founded in October 2016 by Carine, Sylvain and Geoffroy, e-Zyvec is a Biotech company devised by scientists to serve scientists. Rather to provide another service of ‘customized’ DNA molecules to biologists, we aim to offer an efficient and innovative partnership in genetic engineering projects. This results in uniquely designed DNA vectors that will truly enhance Life Science research.

Our Services (23)


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sgRNA Synthesis

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Lentiviral sgRNA Library Construction

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AAV Production

Adeno-associated virus (AAV) production
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Vector Construction and Optimization

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100% Sequence Guaranteed

Any authorized biological system

Available Vector Systems

·       Gene Expression Vectors

·       Non-coding RNA Expression Vectors

·       shRNA Knockdown Vectors

·       CRISPR Vectors

·       HR Donor Vectors (for CRISPR Targeting)

·       Enhancer/Promoter Testing Vectors

·       Recombinant Protein Expression Vectors

·       In Vitro Transcription Vectors

·       And more!

Modify/Optimize existing vectors

·       Simply provide us with a vector map and/or sequence for your vector, and we will be happy to modify your existing vector to insert or remove any number of features upon request


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shRNA Plasmid Construction

Price on request

              Mammalian shRNA Knockdown Vectors
U6-Based

·       Lentivirus

·       Adeno-associated virus (AAV)

miR30-Based

·       Regular plasmid

·       Lentivirus

·       Adeno-associated Virus (AAV)


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rAAV Genome Editing

Recombinant Adeno-Associated Virus Mediated Genome Editing
Price on request
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rAAV Production

Recombinant Adeno-Associated Virus Products
Price on request
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Plasmids and Vectors

Price on request

100% Sequence Guaranteed

Any authorized biological system

Available Vector Systems

·       Gene Expression Vectors

·       Non-coding RNA Expression Vectors

·       shRNA Knockdown Vectors

·       CRISPR Vectors

·       HR Donor Vectors (for CRISPR Targeting)

·       Enhancer/Promoter Testing Vectors

·       Recombinant Protein Expression Vectors

·       In Vitro Transcription Vectors

·       And more!

Modify/Optimize existing vectors

·       Simply provide us with a vector map and/or sequence for your vector, and we will be happy to modify your existing vector to insert or remove any number of features upon request


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Plasmid DNA Preparation

Price on request

Endo-free midiprep (DNA)

Miniprep (DNA)


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Molecular Cloning

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Genetic Engineering

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DNA Services

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Custom Lentiviral Vectors

Price on request
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CRISPR/Cas9 Vector Construction

Price on request

gRNA and Cas9 Coexpression
Single gRNA

·       Lentivirus

·       Adeno-associated virus (AAV)

·       Regular plasmid

Dual gRNA

·       Lentivirus

·       Adenovirus

·       Regular plasmid

Multiple gRNA

·       Lentivirus

·       Adenovirus

·       Regular plasmid

 

gRNA Only
Single gRNA

·       Lentivirus

·       Adeno-associated virus (AAV)

·       Regular plasmid

Dual gRNA

·       Lentivirus

Adeno-associated virus (AAV)

·       Regular plasmid

Multiple gRNA

·       Lentivirus

·       Adenovirus

·       Regular plasmid

 

Cas9 Only

·       Lentivirus

·       Adeno-associated virus (AAV)

·       Regular plasmid

 

CRISPR-Based Gene Activation

·       Any Cas protein fused with any Transcription factor

 

CRISPR-Based Eipigenetic control

·       Any Cas protein fused with any DNA modifying enzyme

 

CRISPR-Based Genomic imaging

·       Any Cas protein fused with any fluorescent proteins (repeated 2-3 times)

 

 

HR Donor Vectors (for CRISPR Targeting)

·       KO Donor Vectors

·       KI Donor Vectors


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Codon Optimization

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Cloning Vectors

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When designing a new vector, our in-house software will analyse all the required genetic features and their predicted arrangement, i.e. their position and orientation in the desired vector. Enlisted features are matched to DNA bricks or templates already existing in our virtual database. The software will then help us to design new DNA bricks containing any unmatched features (typically customers’ SOI), so that these new bricks will be fully compatible with the existing ones. By analysing both existing bricks and the SOIs to be included, our software also determines the best-suited of our four standardised assembly protocols.

This means that the mix of DNA bricks and the conditions of assembly are re-calculated for every project to ensure optimal production of vectors.

The actual production is handled in our laboratory. Physical DNA bricks are generated using High Fidelity PCR on appropriate DNA templates (either from our customers or providers) prior to vector assembly. Our optimised assembly conditions allow us to routinely assemble several 100 to 8000bp-long DNA bricks (up to 12 different bricks) in a seamless fashion, to generate circular DNA molecules (vectors) generally ranging from 4 to 15kb.

As each single molecule is obtained from a unique mix of DNA bricks, several vectors can be assembled in parallel in series of tubes just differing by the mix of DNA bricks they contain.


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cDNA Cloning

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Bacterial Artificial Chromosome (BAC)

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BAC Generation

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Fusion Proteins

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Mutagenesis

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Protein Engineering/Directed Evolution

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Genome Editing

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SJ

Sylvain Julien

Chairman

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