1352601184186_7fffb663120dd173020ed4b9bc223583

DNA Sequencing Facility

Philadelphia, Pennsylvania
1352601184186_7fffb663120dd173020ed4b9bc223583
DNA Sequencing FacilityPerelman School of MedicinePhiladelphia, Pennsylvania
  • 17 Services
  • 2 Members

The DNA Sequencing Facility is an Abramson Cancer Center Shared Resource that is approved and partially funded by the National Cancer Institute.

    Cell transfection
    $40.00 USD
    per Sample

    Contact for detailed pricing information.

    Contact for detailed pricing information.

    « Show less
    Genotyping by DNA Sequencing
    Price on request

    Microsatellite-based Genotyping/Fragment Sizing on ABI 3130:
    96 samples (labeled PCR products), w/genotyping $161
    48 samples (labeled PCR products), w/genotyping $103
    96 samples RTL(samples+loading mix on ABI plate), w/genotyping $103
    48 samples RTL(samples+loading mix on ABI plate), w/genotyping $66 Show more »

    Microsatellite-based Genotyping/Fragment Sizing on ABI 3130:
    96 samples (labeled PCR products), w/genotyping $161
    48 samples (labeled PCR products), w/genotyping $103
    96 samples RTL(samples+loading mix on ABI plate), w/genotyping $103
    48 samples RTL(samples+loading mix on ABI plate), w/genotyping $66
    Also available are: full service genotyping (including PCR, pooling, sizing and genotyping), LOH Analysis, SNaPshot, and
    mouse genotyping (for transgene identification) please contact us.

    « Show less
    Genotyping by PCR
    $1.68 USD
    per Sample

    Microsatellite-based Genotyping/Fragment Sizing on ABI 3130:
    96 samples (labeled PCR products), w/genotyping $161
    48 samples (labeled PCR products), w/genotyping $103
    96 samples RTL(samples+loading mix on ABI plate), w/genotyping $103
    48 samples RTL(samples+loading mix on ABI plate), w/genotyping... Show more »

    Microsatellite-based Genotyping/Fragment Sizing on ABI 3130:
    96 samples (labeled PCR products), w/genotyping $161
    48 samples (labeled PCR products), w/genotyping $103
    96 samples RTL(samples+loading mix on ABI plate), w/genotyping $103
    48 samples RTL(samples+loading mix on ABI plate), w/genotyping $66

    Also available are: full service genotyping (including PCR, pooling, sizing and genotyping), LOH Analysis, SNaPshot, and
    mouse genotyping (for transgene identification) please contact us.

    « Show less
    Ion Torrent NGS
    Ion Torrent next generation sequencing
    $600.00 USD
    per Sample

    gDNA and long amplicon: $600 - $995
    User prepared amplicons: $515 - $920
    Cancer Panel v2 (incl. 1 library prep) $700 - $900

    gDNA and long amplicon: $600 - $995
    User prepared amplicons: $515 - $920
    Cancer Panel v2 (incl. 1 library prep) $700 - $900

    « Show less
    Ion Torrent library generation
    $80.00 USD
    per Sample

    Barcoded library (gDNA, long amplicons, 4 minimum) - $80 each

    AmpliSeq cancer or custom panel (4 minimum) - $170 each

    Barcoded library (gDNA, long amplicons, 4 minimum) - $80 each

    AmpliSeq cancer or custom panel (4 minimum) - $170 each

    « Show less
    Methylation analysis
    Price on request

    Bisulphite sequencing

    Bisulphite sequencing

    « Show less
    Microsatellite sequencing
    $103.00 USD
    per Run

    Microsatellite-based Genotyping/Fragment Sizing on ABI 3130:
    96 samples (labeled PCR products), w/genotyping $161
    48 samples (labeled PCR products), w/genotyping $103
    96 samples RTL(samples+loading mix on ABI plate), w/genotyping $103
    48 samples RTL(samples+loading mix on ABI plate), w/genotyping $66 Show more »

    Microsatellite-based Genotyping/Fragment Sizing on ABI 3130:
    96 samples (labeled PCR products), w/genotyping $161
    48 samples (labeled PCR products), w/genotyping $103
    96 samples RTL(samples+loading mix on ABI plate), w/genotyping $103
    48 samples RTL(samples+loading mix on ABI plate), w/genotyping $66
    Also available are: full service genotyping (including PCR, pooling, sizing and genotyping), LOH Analysis, SNaPshot, and
    mouse genotyping (for transgene identification) please contact us.

    « Show less
    NGS data analysis
    Next generation sequencing data analysis
    $100.00 USD
    per Hour

    Bioinformatic analysis of NGS data.

    Bioinformatic analysis of NGS data.

    « Show less
    PCR
    Polymerase chain reaction
    $9.50 USD
    per Sample

    PCR amplification of template (1-3 samples): $13.20
    PCR amplification of template (> 4 samples): $9.50
    PCR product / fragment purification (Ampure): $9.10
    PCR product purification (column): $9.70
    PCR product purification (enzymatic): $2.40
    PCR product purification (gel): $30.60

    PCR amplification of template (1-3 samples): $13.20
    PCR amplification of template (> 4 samples): $9.50
    PCR product / fragment purification (Ampure): $9.10
    PCR product purification (column): $9.70
    PCR product purification (enzymatic): $2.40
    PCR product purification (gel): $30.60

    « Show less
    PCR Purification
    $2.40 USD
    per Sample

    PCR product / fragment purification (Ampure): $9.10
    PCR product purification (column): $9.70
    PCR product purification (enzymatic): $2.40
    PCR product purification (gel): $30.60

    PCR product / fragment purification (Ampure): $9.10
    PCR product purification (column): $9.70
    PCR product purification (enzymatic): $2.40
    PCR product purification (gel): $30.60

    « Show less
    Plasmid DNA extraction
    $5.50 USD
    per Sample

    Plasmid Miniprep on Qiacube (Qiagen) $5.50/sample
    Plasmid or phage Midiprep (1-3 samples) $56/sample
    Plasmid or phage Midiprep (4 or more samples) $39/sample
    Plasmid or phage Maxiprep (1-3 samples) $70/sample
    Plasmid or phage Maxiprep (4 or more samples) $56/sample
    Plasmid or phage Mega prep (1-3... Show more »

    Plasmid Miniprep on Qiacube (Qiagen) $5.50/sample
    Plasmid or phage Midiprep (1-3 samples) $56/sample
    Plasmid or phage Midiprep (4 or more samples) $39/sample
    Plasmid or phage Maxiprep (1-3 samples) $70/sample
    Plasmid or phage Maxiprep (4 or more samples) $56/sample
    Plasmid or phage Mega prep (1-3 samples) $125/sample
    Plasmid or phage Mega prep (4 or more samples) $101/sample
    __________

    DNA extraction from plasmids in 96-well plates $205/plate
    _________

    Template (Plasmid) Preparation Including Transformation & Purification

    Template DNA can be prepared and/or purified by the facility staff. Plasmid, cosmid, lambda phage, and BAC preps are offered through the Sequencing Facility. PCR reactions and purification using commercial columns is also offered by the facility. Plasmid minipreps are done on the Qiagen Qiacube. These preps yield 5-10 μg DNA for high copy number plasmids. If used for sequencing, the Facility guarantees the results, i.e. we will repurify and repeat sequencing at no additional charge until successful sequence is obtained.

    Transformation service is provided is to make a large prep from a small amount of left-over DNA.

    The facility also offers reliable treatment of marginal or bad templates to make them suitable for automated sequencing. This involves RNase treatment followed by column purification or phenol-chloroform extraction. This treatment improves quality of template, producing more reliable sequence and longer reads. For primer walking projects we recommend that a substantial amount of DNA (nominally 4-5 μg per kb for each direction) be treated this way and be used for the whole project.

    Templates prepared or purified by the facility staff are guaranteed to produce good sequence with standard, reliable primers, barring sequence specific problems (GC rich templates, secondary structure, internal repeats). The facility also provides free training in template preparation and troubleshooting, at users' request.

    Fees:
    DNA extraction from plasmids in 96 well plate:
    SOM, Cancer Center, PGFI members $175
    Other Penn, CHOP, Wistar $190
    Off Campus (non-profit) $205

    « Show less
    Plasmid sequencing
    $6.88 USD
    per Sample

    High Throughput Sequencing from Bacterial Cultures (for high copy plasmids only)

    We prepare plasmid DNA from cultures submitted in a plate and sequence. We can also prepare a culture plate from a glycerol stock plate.

    Full Plate, one end sequenced $660
    Full Plate, both ends sequenced $875

    High Throughput Sequencing from Bacterial Cultures (for high copy plasmids only)

    We prepare plasmid DNA from cultures submitted in a plate and sequence. We can also prepare a culture plate from a glycerol stock plate.

    Full Plate, one end sequenced $660
    Full Plate, both ends sequenced $875

    « Show less
    Roche 454 NGS
    Roche 454 next generation sequencing
    $10,120.00 USD
    per Plate

    Amplicons (customer generated)

    1/4 plate, ~200k reads: $2950
    1/2 plate, ~500k reads: $5390
    full plate, ~1M reads: $10120

    Genomic DNA and long ( > 1.5 kb ) amplicon (including 1 library prep)

    1/4 plate, ~200k reads: $3500
    1/2 plate, ~500k reads: $5940
    full plate, ~1M reads: $11000

    Amplicons (customer generated)

    1/4 plate, ~200k reads: $2950
    1/2 plate, ~500k reads: $5390
    full plate, ~1M reads: $10120

    Genomic DNA and long ( > 1.5 kb ) amplicon (including 1 library prep)

    1/4 plate, ~200k reads: $3500
    1/2 plate, ~500k reads: $5940
    full plate, ~1M reads: $11000

    « Show less
    Roche 454 library generation for next generation sequencing
    $250.00 USD
    per Sample

    Barcoded Rapid Library preparation: $250 each (for 4+ libraries)

    For smaller projects please contact.

    Barcoded Rapid Library preparation: $250 each (for 4+ libraries)

    For smaller projects please contact.

    « Show less
    Sanger Sequencing
    $9.00 USD
    per Sample

    Full Sequencing *$9
    Full plate only $450/plate
    Enhancer (additional reagent ) $0.70

    *price reduced

    Full Sequencing *$9
    Full plate only $450/plate
    Enhancer (additional reagent ) $0.70

    *price reduced

    « Show less
    Site directed mutagenesis
    $320.00 USD
    per Sample

    Site-directed Mutation (mutagenic Oligos & sequencing not included)

    Site-directed Mutation (mutagenic Oligos & sequencing not included)

    « Show less
    Transgenic mouse
    Transgenic mouse development
    $3,200.00 USD
    per Strain

    Targeting Vector Construction for Gene Targeting
    Conditional Knock-out construct $3,800
    Knock-in construct $3,200

    Targeting Vector Construction for Gene Targeting
    Conditional Knock-out construct $3,800
    Knock-in construct $3,200

    « Show less

Shobhit Agrawal gave a positive review for Ion Torrent NGS:

October 18, 2012
very professional, good quality data and rapid completion of project. highly recommended.

Be the first to endorse DNA Sequencing Facility

Endorse this lab