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DNA Sequencing Facility (University of Wisconsin Biotechnology Center)

Madison, Wisconsin, US

The UWBC DNA Sequencing Facility DNA-Sequencing was established to provide cost-effective, cutting edge sequencing resources available to the University of Wisconsin campus as well as colleagues at public and private institutions. We provide comprehensive, fee-for-service support for both Illumina Next Generation and Sanger sequencing projects, from project design and sample preparation through downstream analysis in conjunction with the UWBC Bioinformatics Resource Facility. In addition, we provide DNA extraction, SNP genotyping, and fragment analysis services, and can facilitate custom projects upon request. Our facility is committed to producing high-quality data, maintaining rapid turn-around times, and providing comprehensive support and resources.

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Genomic DNA Extraction
Price on request

The DNA Sequencing Facility provides full-service DNA extraction services for whole blood, saliva, buccal swabs, plant tissue and mammal tissue on a rolling-submission, fee-for-service basis. The UWBC DNA Sequencing Facility can also work with researchers to extract DNA from tissues that may not be listed here. Please inquire... Show more »

The DNA Sequencing Facility provides full-service DNA extraction services for whole blood, saliva, buccal swabs, plant tissue and mammal tissue on a rolling-submission, fee-for-service basis. The UWBC DNA Sequencing Facility can also work with researchers to extract DNA from tissues that may not be listed here. Please inquire about pricing for DNA sources not listed here.

Pricing

Note: non-UW users are charged a 53% surcharge above rates listed below.

Input Setup fee Per Sample fee Example Pricing
Buccal Swabs - Very fresh, bristle-style swabs generally yield the best results. Swabs must be clearly labeled. $190.00 Applied per set of up to 96 swabs $3.75 $550.00 (~$5.73 per extraction) 96 buccal swabs.
Mammal Tissue - Up to 20-mg tissue in 96-well plates. $140.00 Applied to a single 96-well plate $4.25
Oragene Saliva Kits - Contents of clearly labeled Oragene collection vessel. $325.00 Applied per set of up to 192 collection vessles $2.75 $853.00 (~$4.44 per extraction) 192 Oragene collection vessels.
Plant Tissue - 1-cm leaf cutting, fresh or lyophilized, in 96-well plates. $180.00 Applied to a single 96-well plate. $1.50 $324.00 (~$3.38 per extraction) One 96-well plate of tissue.
Whole Blood (Low-throughput) - Up to 10-mL whole blood in clearly labeled vials. $130.00 Applied per set of up to16 vials. $16.00 $386.00 (~$24.13 per extraction) 16 vials, whole blood (up to 10-mL).
Whole Blood (High-throughput) - 200-uL whole blood in clearly labeled vials. $700.00 Applied per set of up to 192 vials. $2.15 $1,112.80 (~$5.80 per extraction) 192 vials, whole blood (up to 200-uL).

Policy Statement

Every effort is made to provide our users with the best data in a timely fashion for the advancement of their research. Costs and turnaround times for DNA extraction projects are dependent on the the specific type of tissue and the number of samples involved. These can be estimated for projects involving tissues for which we have already optimized DNA extraction protocols.

All data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequence Facility are intended for research purposes only. They are not intended or certified for diagnostic or clinical use.

Acknowledging UWBC and the DNA Sequencing Facility

A visible measure of a core's impact is made through proper acknowledgement of usage in publications. To acknowledge our core, please use the following statement: "The author(s) thank the University of Wisconsin Biotechnology Center DNA Sequencing Facility for providing ________ facilities and services”.

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Illumina NGS Library Preparation
Price on request

The DNA Sequencing Facility offers all-inclusive next generation sequencing, DNA extraction and SNP genotyping services on a rolling-submission, fee-for-service basis, with the goal of generating high-quality genomic and transcriptomic data. We work with our UW system and external collaborators to prepare DNA, RNA, ChIP, GBS,... Show more »

The DNA Sequencing Facility offers all-inclusive next generation sequencing, DNA extraction and SNP genotyping services on a rolling-submission, fee-for-service basis, with the goal of generating high-quality genomic and transcriptomic data. We work with our UW system and external collaborators to prepare DNA, RNA, ChIP, GBS, metagenomic, exome, and other sequencing libraries, provide robust sequencing data, and help interpret that data to provide meaningful biological information in a time-sensitive manner.

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Illumina Sequencing
Price on request

The DNA Sequencing Facility offers all-inclusive next generation sequencing, DNA extraction and SNP genotyping services on a rolling-submission, fee-for-service basis, with the goal of generating high-quality genomic and transcriptomic data. We work with our UW system and external collaborators to prepare DNA, RNA, ChIP, GBS,... Show more »

The DNA Sequencing Facility offers all-inclusive next generation sequencing, DNA extraction and SNP genotyping services on a rolling-submission, fee-for-service basis, with the goal of generating high-quality genomic and transcriptomic data. We work with our UW system and external collaborators to prepare DNA, RNA, ChIP, GBS, metagenomic, exome, and other sequencing libraries, provide robust sequencing data, and help interpret that data to provide meaningful biological information in a time-sensitive manner.

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Biology
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Biochemistry & Molecular Biology
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Nucleic Acid Services
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DNA Services
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DNA Sequencing
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Next Generation Sequencing (NGS)
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DNA Library Construction Services
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DNA Extraction and Purification
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