The DNA Sequencing Center (DNASC) at Brigham Young University was established to help researchers and students process DNA samples efficiently and economically. The DNASC is committed to serving the research community by providing advanced and efficient services for DNA sequence and DNA fragment acquisition and analysis.
Some of the services provided by the DNASC include:
High MW DNA genomic libraries are constructed. We're happy to work with you in isolating HMW DNA for the library construction. Yield per SMRTcell depends a bit on saturation. Higher saturation provides more data, but often the average read lengths are slightly shorter.
We also make iso-seq libraries from the RNA samples that you send us. The higher the intactness of the RNA, the better.
We're also making bar coded capture libraries with 6Kb average sizes, and then pooling multiple samples per SMRTcell
Illumina TruSeq DNA PCR-Free Sample Prep $150.00
Illumina TruSeq DNA Nano Sample Prep $150
Nextera Mate Pair library constructions $600 for the first library, $120 for subsuquent cuts from the same preparation.
Other preparation kits available
Illumina HiSeq 2500 High-Output v4 Sequencing
HiSeq 125 Cycle Paired-End Sequencing v4/ $2,490
HiSeq 50 Cycle Single-Read Sequencing v4/ $1,287
Illumina HiSeq 2500 Rapid Run v2 Sequencing
HiSeq 500 Cycle Paired-End (250 cycles from each end) Sequencing v2/ $3620
HiSeq 300 Cycle Paired-End (150 cycles from each end) Sequencing v2/ $2891
HiSeq 200 Cycle Paired-End (100 cycles from each end) Sequencing v2/ $2288
HiSeq 200 Cycle Single Read (200 cycles from one end) Sequencing v2/ $2012
HiSeq 150 Cycle Paired-End (75 cycles from each end) Sequencing v2/ $2095
HiSeq 150 Cycle Single Read (150 cycles from one end) Sequencing v2/ $1820
HiSeq 100 Cycle Paired-End (50 cycles from each end) Sequencing v2/ $1794
HiSeq 100 Cycle Single Read (100 cycles from one end) Sequencing v2/ $1518
HiSeq 50 Cycle Single Read (50 cycles from one end) Sequencing v2/ $1225
Rapid runs typically can be turned around in less than a week, especially with advanced notification. High output runs can sometimes take up to 40 days depending on many lanes you are requesting. We only run high output 50 cycle Single-read lanes if you can provide samples to fill all 8 lanes.
Illumina TruSeq RNA Sample Prep v2 with oligo dT selection $160
Illumina TruSeq Stranded Total RNA Sample Prep with Ribo-Zero Gold $200
Illumina TruSeq Stranded Total RNA Sample Prep with Ribo-Zero (plant) $220
Illumina TruSeq Stranded Total RNA Sample Prep with Ribo-Zero (yeast) $290
Kapa Stranded mRNA-Seq Kit for Illumina $150
There are discounts of 10% or more when submitting more than 40 samples. The more RNA of the highest quality that you can send, the better will be the results that we can return to you. We would like 4ug of total RNA for the fewest possible PCR cycles using the Kapa kit. Turnaround times vary on the number of samples, but usually happens within 30-40 days.
"Competitive pricing structure, very timely turnaround, and very cooperative staff."
DNA Sequencing Center has not received any endorsements.