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Digital Proteomics LLC

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1 Order Completed
San Diego, California, US

About Digital Proteomics LLC

Digital Proteomics is a unique CRO offering antibody sequencing and discovery services.  We bring sophisticated algorithmic and machine learning approaches to enable efficient and accurate analysis of monoclonal and polyclonal antibodies.

Our Services (7)


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Hybridoma Sequencing

Starting at $100.00 per sample

High-throughput sequencing of hybridoma variable regions using next-generation sequencing.

Features:

  • Full-length variable region sequencing of all clones
  • Optimized for high-throughput sequencing
  • Available for mouse, rabbit, and human.  Other species available on request!
  • Only 40,000 cells per hybridoma needed!

We deliver:

  • Full-length variable regions for heavy chain and light chain for each hybridoma
  • Report on minor clones for each hybridoma
  • Available for mouse, rabbit, and human.

 

Turn around time: 3 weeks or less

 

Pricing for 1 96-well plate: $100/mAb

Next Generation Sequencing Illumina MiSeq Mouse Humanized Mouse Human Rabbit Show 6 more tags Show less

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Antibody Development

Starting at $20,000.00 per study

You know the serum has the reactivity you need for you assay, so why look anywhere else for monoclonal antibodies?

Our proprietary Alicanto platform discovers natural, high affinity antibodies without creating a library. We use mass spectrometry-based analysis to deconvolve the polyclonal serum antibodies into monoclonals. 

Starting material:

  • 2.5 mg of antigen


Process:

  1. Animal(s) are immunized with client-provided antigen.
  2. B cell-containing samples are collected during immunization and analyzed by next-generation sequencing.
  3. Serum antibodies are affinity purified against the client-provided antigen and subjected to mass spectrometry analysis.
  4. Next-generation sequencing data and mass spectrometry data are analyzed by Alicanto discovery software to identify antigen-specific antibodies present in serum.

 

Deliverables:

  • At least 10 antibody sequences selected by Alicanto.
  • Full access to all antibody sequences detected.

 

Timeline - up to 20 weeks:

  • Immunization: 14 weeks
  • Data generation (NGS and LS-MS/MS): 4 weeks
  • Data analysis: 2 weeks

 

Species:

  • Rabbit monoclonal antibodies
  • Llama single chain antibodies
  • Alpaca single chain antibodies
  • Human monoclonal antibodies

 

Ask us about

  • customized services
  • working with your existing immunizations
  • working with different species
  • expressing the Alicanto-discovered antibody sequences


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Immune Repertoire Sequencing

Starting at $800.00 per library

The immune repertoire is a snapshot of an individual's historical as well as acute response to immunological challenge. We offer B-cell receptor repertoire sequencing using next-generation sequencing together with our proprietary Reptor platform.

Species:

  • Mouse
  • Human
  • Rabbit
  • Llama
  • Alpaca
  • Goat
  • Other species, including transgenics, available on request!


Sample requirements: 

  • Tissue or cells
  • cDNA
  • phagemids
  • hybridomas


Deliverables:

  • Complete access to all antibody sequences
  • An interactive Reptor server for visualizing clonal lineages, CDR sequences, andgermline gene usage. 


Turnaround time:  As little as 3 weeks!

 

Illumina MiSeq Illumina MiSeq Human Mouse Rabbit Llama Alpaca Goat Camelid Camelids Show 10 more tags Show less

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Antibody Protein Sequencing

Starting at $5,000.00 per sample

Lost your hybridoma? We can recover the full sequence of your antibody of any isotype from any species.

Starting material:

  • 100-200ug of 95% purity monoclonal antibody

Deliverables:

  • Full length heavy and light chain sequences.
  • Coverage report for each amino acid in the predicted sequences.
  • Isoleucine/Leucine determination using w-ions
  • Guaranteed full spectral coverage.

Turnaround time: 4 weeks

Bulk pricing available!

We've been sequencing antibody proteins for over 10 years! Ask us about our current promotions!

HPLC LC/MS/MS Proteomics Mass Spec Human Mouse Rat Hamster Rabbit Show 9 more tags Show less

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De Novo Antibody Sequencing

Starting at $5,000.00 per sample

Lost your hybridoma? We can recover the full sequence of your antibody of any isotype from any species.

Starting material:

  • 100-200ug of 95% purity monoclonal antibody

Deliverables:

  • Full length heavy and light chain sequences.
  • Coverage report for each amino acid in the predicted sequences.
  • Isoleucine/Leucine determination using w-ions
  • Guaranteed full spectral coverage.

Turnaround time: 4 weeks

Bulk pricing available!

We've been sequencing antibody proteins for over 10 years! Ask us about our current promotions!

Mass Spectrometry Antibodies Mouse Rat Hamster Rabbit Human HPLC LC/MS/MS Proteomics Show 10 more tags Show less

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Next Generation Sequencing (NGS)

Starting at $800.00 per library

The immune repertoire is a snapshot of an individual's historical as well as acute response to immunological challenge. We offer B-cell receptor repertoire sequencing using next-generation sequencing together with our proprietary Reptor platform.

Species:

  • Mouse
  • Human
  • Rabbit
  • Llama
  • Alpaca
  • Goat
  • Other species, including transgenics, available on request!

Sample requirements: 

  • Tissue or cells
  • cDNA
  • phagemids
  • hybridomas

Deliverables:

  • Complete access to all antibody sequences
  • An interactive Reptor server for visualizing clonal lineages, CDR sequences, andgermline gene usage. 

Turnaround time:  As little as 3 weeks!

 

Illumina MiSeq Next Generation Sequencing Bioinformatics Human Rabbit Camelids Goat Illumina MiSeq Mouse Llama Alpaca Show 11 more tags Show less

ic

Antibody Discovery

Starting at $20,000.00 per study

You know the serum has the reactivity you need for you assay, so why look anywhere else for monoclonal antibodies?

Our proprietary Alicanto platform discovers natural, high affinity antibodies without creating a library. We use mass spectrometry-based analysis to deconvolve the polyclonal serum antibodies into monoclonals. 

Starting material:

  • 2.5 mg of antigen

 

Process:

  1. Animal(s) are immunized with client-provided antigen.
  2. B cell-containing samples are collected during immunization and analyzed by next-generation sequencing.
  3. Serum antibodies are affinity purified against the client-provided antigen and subjected to mass spectrometry analysis.
  4. Next-generation sequencing data and mass spectrometry data are analyzed by Alicanto discovery software to identify antigen-specific antibodies present in serum.

 

Timeline - up to 20 weeks:

  • Immunization: 14 weeks
  • Data generation (NGS and LS-MS/MS): 4 weeks
  • Data analysis: 2 weeks

 

Deliverables:

  • At least 10 antibody sequences selected by Alicanto.
  • Full access to all antibody sequences detected.

 

Species:

  • Rabbit monoclonal antibodies
  • Llama single chain antibodies
  • Alpaca single chain antibodies
  • Human monoclonal antibodies

 

Ask us about

  • customized services
  • working with your existing immunizations
  • working with different species
  • expressing the Alicanto-discovered antibody sequences

Camelids Rabbit Human Humanized Rat Humanized Mouse LC/MS/MS HPLC Illumina MiSeq Llama Alpaca Show 10 more tags Show less

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Natalie Castellana

Chief Executive Officer

FileType/PDF Created with Sketch. DigitalProteomics_Alicanto_AlpacaMabDiscovery.pdf

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FileType/PDF Created with Sketch. DigitalProteomics_Alicanto_RabbitMabDiscovery.pdf

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FileType/PDF Created with Sketch. DigitalProteomics_Reptor_RepertoireSequencing.pdf

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FileType/PDF Created with Sketch. DigitalProteomics_Valens_MabSequencing.pdf

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