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Desktop Genetics

London, GB

Desktop Genetics supplies AI-designed CRISPR libraries for target discovery, validation and functional genomics. Built on the largest database of validated gRNAs, the DESKGEN AI uses machine learning to build the most advanced models of CRISPR gRNA activity and specificity. These model allows for the design and building of bespoke gRNA libraries, tailored to the species, cell type or disease investigated.

Desktop Genetics has not listed any services.

Pooled Custom Lentiviral CRISPR Libraries
Price on request

Desktop Genetics specialises in the design of custom high-quality libraries for CRISPR screening. We work with a network of expert partners to synthesise and assemble the highest quality reagents based on our designs. These partnerships allow us to tailor every library to your specific needs without incurring high customisation... Show more »

Desktop Genetics specialises in the design of custom high-quality libraries for CRISPR screening. We work with a network of expert partners to synthesise and assemble the highest quality reagents based on our designs. These partnerships allow us to tailor every library to your specific needs without incurring high customisation costs. With our pooled oligonucleotide CRISPR libraries, you have full control over the vector and viral production. We will design and synthesise the oligos to your specifications, using our machine learning-based design pipeline, and ship you the oligos for cloning into your vector of choice.

Using pooled CRISPR libraries, you can screen thousands of gene knock-outs in one experiment. Screening a pool of sgRNAs enables very high throughput functional genomics screening at a low cost. There is no need to screen thousands of constructs using expensive or complex liquid-handling robots.

Design:
Library designed using Desktop Genetics's machine-learning based sgRNA design algorithm.
sgRNAs are designed with minimal off-target effects and maximal activity.
Entire library is design by taking into consideration multiple published and un-published parameters.
sgRNAs can be designed to target protein functional domains, promoter regions, splice junctions, etc. - completely customised to your targets and cell line.

DNA:
Synthesized using Twist Bioscience's high-throughput oligonucleotide synthesis platform.
High quality oligo pool without randomizations and with minimal mutation rates (>0.2%).
Representation is maintained with massively parallel high throughput synthesis method to ensure similar quantities of each sgRNA oligonucleotide in the pool.

Vector:
Chose from an All-In-1 vector system or split Cas9/sgRNA 2-vector system.
Chose from constitutive or inducible (TET-ON3G) promoters for expression of Cas9 or sgRNA.
All vectors include dual selection markers (GFP & PuroR).
Pooled cloning is validated using NGS-assay to ensure maintained complexity and representation of sgRNA population.

Lentivirus:
Manufacture using Vectalys's high-quality production pipeline
Very high lentivirus allow for efficient delivery of sgRNA libraries into a wide range of cell types.
Tailor the purity and concentration of the lentivirus pools to your cell types (10E6 - 10E9 TU/mL)
Transduce primary cells with our VectaPremium Lentivirus.

Analysis:
Each sgRNA serves as an unambiguous sequenceable barcodes.
Screening assay is based on NGS sequencing of amplified gDNA from pool of cells.
Desktop Genetics can assist you in the analysis and deconvolution of your screening results.

Deliverables:
- design manifest file (CSV of all CRISPR sgRNAs and their scores, associated genes, and all properties);
- lentivirus pool, ready for transduction;
- NGS quality report on lentivirus library with representation and complexity analysis.

Turnaround time:
Approx. 3-4 months from order.

Price:
Please enquire for a quote.
Our prices depend on the size and complexity of the library to be designed and manufactured, and the final purity, volume and concentration of the lentivirus manufactured.
We also offer discounts for collaborative projects.

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Custom CRISPR Libraries
Price on request

Desktop Genetics specialises in the design of custom high-quality libraries for CRISPR screening. We work with a network of expert partners to synthesise and assemble the highest quality reagents based on our designs. These partnerships allow us to tailor every library to your specific needs without incurring high customisation... Show more »

Desktop Genetics specialises in the design of custom high-quality libraries for CRISPR screening. We work with a network of expert partners to synthesise and assemble the highest quality reagents based on our designs. These partnerships allow us to tailor every library to your specific needs without incurring high customisation costs. With our pooled oligonucleotide CRISPR libraries, you have full control over the vector and viral production. We will design and synthesise the oligos to your specifications, using our machine learning-based design pipeline, and ship you the oligos for cloning into your vector of choice.

Using pooled CRISPR libraries, you can screen thousands of gene knock-outs in one experiment. Screening a pool of sgRNAs enables very high throughput functional genomics screening at a low cost. There is no need to screen thousands of constructs using expensive or complex liquid-handling robots.

Design:
Library designed using Desktop Genetics's machine-learning based sgRNA design algorithm.
sgRNAs are designed with minimal off-target effects and maximal activity.
Entire library is design by taking into consideration multiple published and un-published parameters.
sgRNAs can be designed to target protein functional domains, promoter regions, splice junctions, etc. - completely customised to your targets and cell line.

DNA:
Synthesized using Twist Bioscience's high-throughput oligonucleotide synthesis platform.
High quality oligo pool without randomizations and with minimal mutation rates (>0.2%).
Representation is maintained with massively parallel high throughput synthesis method to ensure similar quantities of each sgRNA oligonucleotide in the pool.

Deliverables:
- design manifest file (CSV of all CRISPR sgRNAs and their scores, associated genes, and all properties)
- lentivirus pool, ready for transduction
- NGS quality report on lentivirus library with representation and complexity analysis

Turnaround time:
Approx. 4 weeks from order.

Price:
Please enquire for a quote. Our prices depend on the size and complexity of the library to be designed and manufactured. We also offer discounts for collaborative projects.

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Human
Mouse
CRISPR
gene editing
Screening
DESKGEN
CRISPR Experiment Design
Price on request

Use the latest algorithms to design your CRISPR genome editing experiment - single guides, nickase pairs, different Cas9 orthologues, donors and more.

Use the latest algorithms to design your CRISPR genome editing experiment - single guides, nickase pairs, different Cas9 orthologues, donors and more.

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CRISPR sgRNA Libraries
Price on request

Design custom high-quality CRISPR sgRNA libraries, specific to your cell line and experiment, that take SNPs, copy number variations, and other genomic variations of your cell line into account.

Swap out the promoter, nuclease, guide scaffold, and other vector elements to get the best library for your experiment.

Don't... Show more »

Design custom high-quality CRISPR sgRNA libraries, specific to your cell line and experiment, that take SNPs, copy number variations, and other genomic variations of your cell line into account.

Swap out the promoter, nuclease, guide scaffold, and other vector elements to get the best library for your experiment.

Don't settle for the same library as everyone else. Our libraries are tailored to meet the specific needs of the experiment.

Take advantage of a composite scoring system that balances the activity, specificity, and cut site location of every guide RNA in the library.

We understand your needs and then handle every vendor and service provider for your library, from design to data analysis.

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CRISPR Bioinformatics
Price on request
  • Find the exact plasmid you're looking for with the most sophisticated DNA search engine around.
  • Upload your plasmids, linear fragments, oligos, guides, and donors.
  • Manage your personal catalog of DNA features and automatically annotate all your sequences.
  • When you need to clone, instantly find the fragments you need in seconds.
  • Find the exact plasmid you're looking for with the most sophisticated DNA search engine around.
  • Upload your plasmids, linear fragments, oligos, guides, and donors.
  • Manage your personal catalog of DNA features and automatically annotate all your sequences.
  • When you need to clone, instantly find the fragments you need in seconds.
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High Throughput Screening
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Deep Sequencing
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Amplicon Sequencing
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Activity Prediction
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Whole Genome Sequencing
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Targeted Sequencing
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Sequencing of PCR Amplicons
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NGS data analysis
Next generation sequencing data analysis
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Machine Learning
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Genome Editing
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Data modeling
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Bioinformatics
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Algorithm Development
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Biochemistry & Molecular Biology
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Nucleic Acid Analysis
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Computational & Statistical Analysis
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Computational Modeling
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Biostatistics & Bioinformatics
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Next Generation Sequencing (NGS)
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Hit Identification
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Nucleic Acid Data Analysis
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Targeted Gene Sequencing
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DNA
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DNA Sequencing
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Drug Discovery
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Drug Discovery & Development
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Genetic Engineering
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Sequencing Data Analysis
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Nucleic Acid Services
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2018-02-07 16:49:56 +0000

Net Promoter Score of 8 received for Custom CRISPR Libraries.

Additional Ratings: satisfaction with deliverable: 8, satisfaction with timeliness: 7.

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