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DefiniGEN

4 Orders Completed
Babraham, GB

About DefiniGEN

DefiniGEN was founded in April 2012 to industrialize the OptiDIFF stem cell production platform developed at the University of Cambridge, UK.

The company has world-leading expertise in the area of iPSC production and metabolic disease modelling. The application of these technologies in drug discovery provides pharmaceutical companies with more predictive in vitro cell...

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DefiniGEN was founded in April 2012 to industrialize the OptiDIFF stem cell production platform developed at the University of Cambridge, UK.

The company has world-leading expertise in the area of iPSC production and metabolic disease modelling. The application of these technologies in drug discovery provides pharmaceutical companies with more predictive in vitro cell products enabling the development of safer and more effective treatments. In addition the technology platform utilises fully defined and humanized conditions required for the development of regenerative medicine cellular therapies.

DefiniGEN builds on intellectual property and knowledge resident at the University of Cambridge Regenerative Medicine Department at Addenbrooke’s Hospital and in addition has in-licensed the Yamanka induced pluripotent stem cell IP portfolio from iPS Academia Japan Inc.

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Our Services (17)


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Cell Line Generation

Price on request

Gene-editing Custom Cell Line Development

  • Enables the introduction of any desired genetic mutation into iPS lines using CRISPR technology
  • Generates highly functional human liver, pancreatic, lung and other cell types from the iPS lines
  • Customer receives isogenic differentiated cell products identical apart from the... Show more »

Gene-editing Custom Cell Line Development

  • Enables the introduction of any desired genetic mutation into iPS lines using CRISPR technology
  • Generates highly functional human liver, pancreatic, lung and other cell types from the iPS lines
  • Customer receives isogenic differentiated cell products identical apart from the desired disease mutation
  • Enables detailed studies of normal and disease biology in monogenic and complex diseases such as diabetes type 2

DefiniGEN’s iPS Differentiation service utilizes our proprietary OptiDIFFTM platform to generate terminally differentiated custom cell products to the client specification. The OptiDIFF technology was developed from world-leading research undertaken at the University of Cambridge and has generated a range of cell types including liver, pancreatic and lung cells. This platform has been combined with the market-leading CRISPR gene-editing technologies, provided by Horizon Discovery Ltd, to offer a unique resource for custom cell line development. This enables the precise introduction of any desired mutation and the rapid production of optimized disease models of monogenic and complex diseases, including diabetes and NAFLD. Ultimately the client receives fully differentiated rigorously QC cell products, which are genotype and phenotype verified in a highly relevant physiological background.

  • CRISPR Design and Validation vector design, synthesis and validation
  • CRISPR-iPS cell Generation electroporation, screening, verification, expansion and banking
  • Differentiation using the OptiDIFF platform
  • Cryopreservation and banking of the differentiated cell products
  • Product Validation – genotype, phenotype, and cell marker analysis
  • Cryopreserved Disease-modelled Differentiated Cells generated from CRISPR gene-edited iPS

The service has produced functional hepatocytes displaying the desired example A1AT mutation generated by CRISPR gene-editing of iPS cell line. Referring to Figure 2. a) Relative expression of key hepatocyte maturity markers via TaqMan® gene expression assays (Applied Biosystems). The mRNA expression levels of albumin (ALB), HNF4α, and α1-antitrypsin (A1AT) were quantified in cryopreserved Def-HEP 10 days post thaw (Cryo) versus fresh cells. Statistical analysis via a one-tailed T-test shows non-statistically significant differences observed between the cryopreserved and non-cryopreserved data groups.

iPSC Differentiation Custom Cell Line Development

DefiniGEN’s iPSC Differentiation service utilizes our proprietary OptiDIFFTM platform to generate high functionality terminally differentiated custom cell products to the client specification. The OptiDIFF platform was developed from world-leading research undertaken at the University of Cambridge and has been demonstrated on a range of cell types. The client receives fully differentiated rigorously QCed cell products which are genotype and phenotype verified in a highly relevant physiological background 1, 2, 3, 4, 5.

  • iPSC Reprogramming or Sourcing iPSC generation or client provision of iPSC
  • Differentiation using OptiDIFF platform
  • Cryopreservation and banking of the differentiated cells
  • Product Validation – genotype, phenotype, and cell marker analysis
  • Delivery of differentiated cryopreserved cells to client with >80% viability post thaw
  • Verification of Function – Cryopreserved Differentiated Cells

See resources figure 2) A relative expression of key hepatocyte maturity markers via TaqMan® gene expression assays (Applied Biosystems). The mRNA expression levels of albumin (ALB), HNF4α, and α1-antitrypsin (A1AT) were quantified in cryopreserved Def-HEP 10 days post thaw (Cryo) versus fresh cells. Statistical analysis via a one-tailed T-test shows non-statistically significant differences observed between the cryopreserved and non-cryopreserved data groups. This data demonstrate that the iPS Differentiation Custom Cell Line Development service can produce highly functional cells to client requirements.

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Cell Line Generation

Price on request

On a contract service basis DefiniGEN can generate IPSC derived liver cells and disease modelled variants to customer specifications using the OPTIDIFF platform. This service can utilise both biological samples provided from client cohort sets and DefiniGEN can also source patients with an inherited metabolic disease of interest using its extensive clinical network.

On a contract service basis DefiniGEN can generate IPSC derived liver cells and disease modelled variants to customer specifications using the OPTIDIFF platform. This service can utilise both biological samples provided from client cohort sets and DefiniGEN can also source patients with an inherited metabolic disease of interest using its extensive clinical network.

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Drug Discovery

Price on request
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Cell and Tissue Culture

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RNA Services

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Mammalian Cell Culture

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Biochemistry & Molecular Biology

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Compound Profiling

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Cells and Tissues

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DNA Extraction and Purification

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Induced Pluripotent Stem Cell (iPSC) Culture

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RNA Extraction and Purification

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Biology

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Drug Discovery & Development

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DNA Services

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Lead Identification and Validation

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Nucleic Acid Services

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  • Positive review for Cell Line Generation:

    May 2018

    "We were very pleased with the service from Definigen."

  • Positive review for Cell Line Generation:

    May 2018

    "Definigen performed a great job and was extremly responsive to questions we had during the project."

DefiniGEN has not received any endorsements.