1351552650514_3aa261241e3f37851fae9d32be991a94

Dana-Farber Cancer Institute

Boston, Massachusetts
1351552650514_3aa261241e3f37851fae9d32be991a94
Dana-Farber Cancer InstituteHarvard Medical SchoolBoston, Massachusetts
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1351552676868_3aa261241e3f37851fae9d32be991a94

Monoclonal Antibody Core Facility (DFCI)

Boston, Massachusetts
  • 100% positive reviews
  • 1 Project Completed
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The Monoclonal Antibody Core at Dana-Farber Cancer Institute produces novel monoclonal antibodies for use in basic research, drug discovery, and clinical applications including diagnosis, surrogate markers for disease status, response to therapy, and drug toxicity. Services include... Show more »

The Monoclonal Antibody Core at Dana-Farber Cancer Institute produces novel monoclonal antibodies for use in basic research, drug discovery, and clinical applications including diagnosis, surrogate markers for disease status, response to therapy, and drug toxicity. Services include cloning and scale-up of immunization antigens to purification of the resulting antibodies. Researchers can choose to have all phases of antibody production conducted by the core or perform particular steps in their own lab.

Services
-Consultation on design of antigens and screening strategies
-Production of vectors and protein with appropriate tags for immunization and screening
-Immunization of animals including mice, rats and hamsters
-Generation of monoclonal antibodies
-Customized screening by WB, ELISA, flow cytometry
-Antibody isotyping, titering, and specificity evaluation
-Small, medium, and large-scale antibody production
-Purification of antibodies
-Antibody productivity assessment
-Cryopreservation and recovery of hybridomas from liquid nitrogen
-Hybridoma banking
-Adaptation of hybridomas to serum-free media
-Mycoplasma testing for hybridomas and other cell lines

Available animal models and cell lines:
We use standard strains of mice, rats and rabbits. Investigators can provide us with knock-out and transgenic animals when they are available. We have a variety of hybridoma fusion partner lines available (SP 2/0; P3X, NS1, YB 2/0, YB 1,2,3, 240E-1).

Equipment:
Biosafety cabinets, CO2 incubators, Molecular Devices Spectromax plate reader, Titertek plate washer, AKTA FPLC systems, access to Biacore and flow cytometers.

Lab members:
Edward Greenfield, PhD (Director); Craig Bencsics (Senior Scientist), Mohan Brahmandam (Scientist) and Yuhua Nong (Scientist).

Most recent publication:
Antbodies: A Laboratory Manual (edited by Edward Greenfield) published by Cold Spring Harbor Press 2013.

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1330040431000

Center for Cancer Computational Biology (CCCB)

Boston, Massachusetts
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Welcome to the Center for Cancer Computational Biology (CCCB) at the Dana-Farber Cancer Institute. The CCCB has three primary elements.

The CCCB sequencing facility offers a wide range of services to assist in the design and execution of next-generation sequencing projects.... Show more »

Welcome to the Center for Cancer Computational Biology (CCCB) at the Dana-Farber Cancer Institute. The CCCB has three primary elements.

The CCCB sequencing facility offers a wide range of services to assist in the design and execution of next-generation sequencing projects. Utilizing the Illumina (Solexa) sequencing technology, we currently support a number of applications inlcuding ChIP-Seq, RNA-Seq, whole genome, whole exome, and targeted re-sequencing.

The analytical services and support platform aims to provide state-of-the-art assistance in the collection, management, analysis, and interpretation of large-scale data with a focus on data generated using 'omic technologies. In addition, we offer software, services, and training designed to assist investigators in advancing their research.

The CCCB research program is focused on development of new methods for improving analysis and interpretation of genomic data through integration of diverse data types with the goal of creating open-source software tools to be made freely-available to the research community.

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1359393200021_f64218da968f2d97767da0086b46d009

Confocal and Light Microscopy core facility (DFCI)

Boston, Massachusetts
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The Confocal and Light Microscopy core facility at Dana Farber Cancer Institute provides state-of-the-art imaging microscopy technology for fixed and live cells and tissues along with scientific and technical expertise to assist in experimental design and optimal image collection. Show more »

The Confocal and Light Microscopy core facility at Dana Farber Cancer Institute provides state-of-the-art imaging microscopy technology for fixed and live cells and tissues along with scientific and technical expertise to assist in experimental design and optimal image collection.
Imaging techniques available through the core include:
Standard fluorescence
Live-cell long term imaging
Confocal
Fluorescence spectral detection
TIRF
FRET
Calcium imaging
Multi-photon
Fluorescence lifetime imaging

Available equipment includes:
Yokogawa spinning disk confocal microscope (Andor)
Leica SP5 laser scanning confocal with a white light laser
Zeiss 710 confocal/multi-photon
Nikon inverted widefield fluorescence microscope

Training on all equipment, consultation about various imaging techniques, and assistance with image analysis is also available. Core staff is also able to assist with troubleshooting microscope issues in individual research labs.

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1392174438077_42190685c691f9e1e6a7cf186754102c

Marasco Laboratory
1392174438077_42190685c691f9e1e6a7cf186754102c
Marasco LaboratoryDana-Farber Cancer InstituteBoston, Massachusetts
  • 1 Service
  • 1 Member

Boston, Massachusetts
1392174438077_42190685c691f9e1e6a7cf186754102c
Marasco LaboratoryDana-Farber Cancer InstituteBoston, Massachusetts
  • 1 Service
  • 1 Member
  • No reviews
  • No completed projects
  • No endorsements

The Marasco Laboratory is located in the Department of Cancer Immunology and AIDS at Dana-Farber Cancer Institute. Our general research interests are in the field of targeted immunotherapy. The Lab’s work has made major scientific advances in the treatment of infectious diseases and... Show more »

The Marasco Laboratory is located in the Department of Cancer Immunology and AIDS at Dana-Farber Cancer Institute. Our general research interests are in the field of targeted immunotherapy. The Lab’s work has made major scientific advances in the treatment of infectious diseases and cancer. More recently, we have been developing humanized mice to support our targeted immunotherapy studies and to investigate the roles of human adult stem cells in regenerative medicine.

Specifically, the lab’s research interests are in human monoclonal antibody (Mab) immunotherapy and broad-spectrum anti-viral vaccine development. We use human Mabs in functional and structural studies to understand mechanisms of viral entry, identify common targets that provide broad-spectrum protection, and develop strategies that prevent the viruses from undergoing neutralization escape. Vaccinated/infected humans and humanized mice are the B-cell sources of antibody genes for these studies.

Our discoveries of common and highly conserved structural elements on the envelope glycoproteins that serve as cross-neutralizing targets for Mab therapy have allowed us to make therapeutic inroads into the prevention and treatment of a wide range of human pathogenic coronaviruses, flaviviruses, and influenza A viruses. The Marasco Laboratory has advanced therapeutic Mab development through NIH-NIAID product development programs in the areas of SARS, West Nile virus, and Influenza A infections. The Lab’s pioneering studies in influenza, which resulted in our finding of a "universal" vaccine target that can provide broad-spectrum protection against a wide range of influenza A viruses, has led to a paradigm shift in the field of influenza immunotherapy and vaccine development.

Our National Foundation of Cancer Research (NFCR) Center for Therapeutic Antibody Engineering (CTAE) at Dana-Farber Cancer Institute is working in a broad range of discovery and translational research in cancer. We have the tools, know-how and expertise to advance our novel immunotherapy discoveries from bench to bedside.

We have a major effort in constructing and improving humanized mice (a.k.a. humouse) and in improving their performance and utility in different biomedical research applications. Our internal research programs are in the areas of human B cell development, optimizing vaccines against infectious agents (influenza A, HIV-1, Denge) and human antibody immunotherapy. We are also using these humanized mice for investigations related to adult stem cell biology and regenerative medicine which are now becoming central efforts in the laboratory. For more details, please visit www.humouse.org.

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