It is increasingly well-recognized that important biological information is spread out throughout the different layers of molecular information, e.g. genetic, biological, biochemical, chemical. Multiomics is a technology that integrates multiple sources of bioinformation to improve the representation of biological systems. Traditionally, this type of study involves splitting samples for separate specialized analyses followed by advanced bioinformatic data integration.
Dalton Bioanalytics offers a multiomic analysis that captures a comprehensive biochemical picture using a single LC-MS assay. Our one-pot, one-shot method enables us to provide multiomic analysis at a fraction of the normal cost while capturing unprecedented diversity of coverage including proteins, lipids, electrolytes, metals, and small molecules (metabolites, drugs, pollutants).
Our platform enables research that is practically impossible otherwise:
Using our technology, we can search for not only single biomarkers but also combinations of biomarkers spanning across different biochemical classes. For instance, the best biomarkers for a given phenotype might be composed of 3 proteins, a small molecule and 2 lipids. This applies to biomarkers for prediction of disease risk, diagnosis, & prognosis, and biomarkers for monitoring disease, therapeutic response, & toxicities.
Multiomic profiling captures a disease biology across many different biological pathways spread across multiple levels of molecular information. For example, a disease may cause alterations in lipid-related processes which can be detected as changes in not only lipid levels, but also lipid-precursor metabolites, lipid-metabolizing co-factors and proteins, and macromolecular lipid-modifications. Collectively these different signals offer not only stronger predictive power but also more biological insights in to the phenotype.
Our approach enables the simultaneous comprehensive examination of biological and chemical responses. Dose-response associations between small molecules or biomolecules and disease pathways within the same samples can elucidate potential therapeutic compounds or targets for further development.
We use high resolution liquid chromatography mass spectrometry combined with our novel assay to provide multiomic analysis spanning across proteins, lipids, electrolytes, metal ions, and small molecules (metabolites, drugs, pollutants). We return these results as a comprehensive analytical report including statistical and bioinformatic analyses with data visualizations (or as raw quantification data).
Our laboratory is at full sample capacity for the next few months, please contact us early if you are interested in launching a multiomic research project. The expected turnaround of two weeks after sample shipment.
Our standard protocol is optimized for 150 microliters of input per sample preparation replicate with a concentration between 10 µg/mL to 100 mg/mL of dry weight. Our analytical platform is compatible with frozen or fresh samples composed of cell or tissue homogenates and biofluids including plasma, serum, cerebrospinal fluid, urine, saliva, cell culture media, etc. Samples should be shipped frozen with no additives, e.g. detergents, polymers, etc. Specific analytes may or may not be compatible with our assay depending on their specific chemistry. Protocols can be adjusted to suite specific analytical needs if required.
Dalton provides can readily provide relative quantification and putative identifications. Absolute quantification and identification of a given analyte requires the availability of chemical reference standards.
Our method can be used to detect features derived from nearly all types of biologically relevant molecules. However, due to the generality of our method, conditions are likely to be suboptimal for any given analyte with typical limits of detection in the micromolar range precluding the detection of low abundance analytes such as specific mRNAs which are generally below the limit of detection. Measurement of a given analyte depends on its in-sample abundance, its chemistry, and the complexity of the sample matrix.
Overall, our services are best suited for examining high to medium abundance proteins, lipids, electrolytes, and small molecules in samples that are readily available such as human plasma. The assay can be adapted for other cases however results may vary.
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Dalton Bioanalytics has not received any endorsements.