Starting from a small lab, it has been more than 10 years since Cusabio was built in 2007. And Cusabio has become a good partner to researchers worldwide. We are dedicated to providing 50,000 validated antibodies, 4900 recombinant proteins, 346 cytokines, 13,000 cDNA clones and thousands of ELISA kits to global customers in the research fields of cancer, cell biology, immunology, neuroscience epigenetics, etc.
The quality of our products is the cornerstone of our ability to serve our clients. For this reason, we invest tremendous resources in developing products, validating their application, managing, controlling and improving quality.
Except for guaranteed quality, Cusabio also offers:
We can provide Polyclonal and monoclonal antibody purification services, the Species of the immune hosts can be rabbit, goat, Guinea pig, chicken, mouse etc. We normally use Traditional antibody purification method such as ammonium sulfate precipitation ,proteinA/G,MEP, immuno affinity chromatography, and if need we can use IE,HIC, MCC, or some other method as your wish. We can preparation antigen binding fragment antibody(Fab) for you too. Purity and activity are the most important factors that affect your experiment result. To resolve your problem we will design the best method with you to purification your antibody according to your antibody’s application.
Monoclonal antibody is that we immuning Balb/C mice with the purpose antigen (protein, polypeptide) designated by our customer, then take the spleen cells, prepare the hybridoma line to produce antibodies specific for antigen with the cell fusion technology. The advantages of monoclonal antibody which is widely used in immunology, medicine, biology and other fields of basic research and clinical medicine are specificity, uniformity, efficiency and an unlimited supply.We engage in antibody production and custom service professionally, provide you with high quality monoclonal antibodies.
Polyclonal antibody exist in immune animal serum and it can get through separate serum directly. Because of it has recognized a multiple tables, can cause aggregation reaction, precipitation reaction and other advantages, and also have a low preparation cost compared with monoclonal antibody preparation. so are widely used in immunology diagnosis field. Such as the antibodies use for the Western blotting experiment and ELISA experiments.
The E. coli expression system is regarded as the most commonly used, economical, and classical expression system because of its simple structure, clear genetic background, high yield of target protein, and its short culture period. In recent decades, E. coli expression system has also been developed and improved continuously, and been used intensively by scientific researchers and industrial users for a large number of recombinant protein expression. The system is mainly used for antigen preparation, ligand preparation, and expression of cytokines and bacteria (Staphylococcus aureus, Escherichia coli, etc.) proteins.
CUSABIO has extensive experience and be very professional in E. coli protein expression and purification. We can solve various difficult problems during the protein expression and purification process. From 2007 to 2017, we have successfully developed more than 4000 recombinant proteins expressed in E. coli, which contain hundreds of active proteins with high purity.
Yeast protein expression system is a highly economical eukaryotic expression system that do both secretion expression and intracellular expression. The exogenous gene expressed by Yeast expression system has a certain post-translational processing capacity, the expressed exogenous protein has a certain degree of folding and glycosylation modification, it’s more stable than prokaryotic expressed proteins, particularly suitable for the expression of eukaryotic genes and preparation of functional proteins. Yeast secretion expression can secrete the expressed exogenous protein into the extracellular matrix, so that it is easy to obtain a high purity protein. Yeast expression system has many advantages which make its research and application more and more widely.
Insect baculovirus expression vector system (BEVS) belongs to the eukaryotic expression system, and it’s an expression system with high safety. It has a large genome, which enables the insertion of large exogenous genes, therefore has the great advantage of expressing proteins with large molecular weight. It also has the ability to achieve complete post-translational modification and efficiently express exogenous genes. The system consists of transfer vector, baculovirus vector and the host cell. The system uses one or more baculovirus super-strong promoters, and gets the recombinant virus after the exogenous target gene is inserted into the promoter. The highly efficient expression of the exogenous gene is achieved while the recombinant viruses replicate themselves in the insect cells. BEVS is widely used in virus vaccine development (such as the development of influenza virus vaccine and HPV vaccine), preparation of cell signaling proteins and cytokines, as well as kinase development, etc.
The prokaryotic expression system has the advantages of high expression level, simple operation, short cycle, easy large-scale and high-density culture and low cost. For the full-length antibody and glycoprotein biological drug, the folding of expression product polypeptide chain, the disulfide bond, the presence or absence of glycosylation and the type of glycosylation often affect the properties of the synthesis, secretion, biological activity, in vivo stability, and immunogenicity of the expressed product. Compared with other eukaryotic expression systems, the expression of the target gene in mammalian cells is similar to that of the native protein in the type and manner of the glycosylation, and can be correctly assembled into the multi-subunit protein.
Purification of native protein always contain several processes, such as Extraction ,clarification ,Intermediate purification,Polishin. Chromatography is the major technology of protein purification,include affinity Chromatography, ion-exchange Chromatography, Size-exclusion chromatography, Hydrophobic Interaction Chromatography and so on .each protein have its particular character ,to obtain a high purity native protein we should choose different purification strategies. if you need a native protein purification service, please contact us ,we will design the experiment with you at the first time, and give the initial quote.
Biochemistry & Molecular Biology Services
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