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Cube Biotech Inc.

Monheim, DE

Structural analysis of membrane proteins is still a huge challenge. This is particularly true for pharmaceutically relevant human membrane proteins, e.g. transporters or G-protein coupled receptors (GPCRs). The design of efficient drugs depends on the availability of membrane protein structure information.

The Cube Biotech team shares more than 45 years of experience on membrane protein expression, purification, and crystallization. We founded Cube Biotech in 2012 to make this experience available to the pharmaceutical and biotech industry, as well as to the academic environment. To ensure the highest quality in our service offering, we at Cube Biotech make our own protein purification resins, membrane protein stabilization and crystallization products. We buy raw materials, cell-free lysates and detergents from selected suppliers dedicated to the highest quality.

Our R+D and production site is located in... Show more »

Structural analysis of membrane proteins is still a huge challenge. This is particularly true for pharmaceutically relevant human membrane proteins, e.g. transporters or G-protein coupled receptors (GPCRs). The design of efficient drugs depends on the availability of membrane protein structure information.

The Cube Biotech team shares more than 45 years of experience on membrane protein expression, purification, and crystallization. We founded Cube Biotech in 2012 to make this experience available to the pharmaceutical and biotech industry, as well as to the academic environment. To ensure the highest quality in our service offering, we at Cube Biotech make our own protein purification resins, membrane protein stabilization and crystallization products. We buy raw materials, cell-free lysates and detergents from selected suppliers dedicated to the highest quality.

Our R+D and production site is located in Monheim, Germany, close to Cologne. We serve customers in many countries from here, and also interact closely with our distributors and OEM partners. In 2013, we founded our US subsidiary in Plymouth Meeting, PA, with a warehouse and customer support team to provide optimal service to our customers in North America.

Whatever we use in our services, we also make available to the community as consumables, accompanied by dedicated protocols and handbooks, tips and tricks for success. As a small company, we are flexible enough to fulfill most needs for small and large volumes, customized resins and kits. Contact us to see how we can support your membrane protein project.

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Nanodisc Technology
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Nanodiscs are an elegant method to stabilize membrane proteins. They provide a detergent-free, phospholipid environment of defined size. Nanodisc-stabilized membrane proteins can be used in a range of biophysical and biochemical assays.

Our service includes a thorough discussion and counselling of your project, so that the... Show more »

Nanodiscs are an elegant method to stabilize membrane proteins. They provide a detergent-free, phospholipid environment of defined size. Nanodisc-stabilized membrane proteins can be used in a range of biophysical and biochemical assays.

Our service includes a thorough discussion and counselling of your project, so that the methods used provide optimal conditions to your protein of interest. Any service package can be booked on its own, or in conjunction with others.

Our services include, but are not limited to:

  • Nanodisc stabilization from detergent-solubilized proteins - as part of an expression project
  • Integration of detergent-solubilized membrane proteins
  • Direct integration of membrane proteins in cell-free reactions
  • Support of your projects with nanodisc products

We offer a wide range of different nanodisc scaffolds such as different nanodisc diameters, scaffolds from different species (human, mouse, rat), scaffolds with and without tags (His and Biotin lable)

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Protein Purification
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Cube Biotech Services for protein purification are open to any small to mid-scale, non-GMP protein purification project, with a focus on the demanding class of membrane proteins. Our service includes a thorough discussion and counselling of your project, so that the methods used provide optimal conditions for the purification of... Show more »

Cube Biotech Services for protein purification are open to any small to mid-scale, non-GMP protein purification project, with a focus on the demanding class of membrane proteins. Our service includes a thorough discussion and counselling of your project, so that the methods used provide optimal conditions for the purification of your protein of interest.

Protein purification methods range from affinity chromatography using various tags including His, Rho1D4, MBP, Strep and others, classical chromatography such as ion exchange and size exclusion for polishing to protein-specific affinity chromatography which takes protein purification to the next level and allows for the enrichment of active protein conformations.

Protein purification can be booked as a separate service package. We can establish protein purification protocols or optimize existing methods. As a starting material, we accept most procaryotic or eucaryotic cell pellets, cell lysates, or cell culture supernatants. Alternatively, the full package of expression optimization and purification is available.

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Protein Crystallization
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Cube Biotech Services for protein crystallization are open to any project, but our focus lies in applications for the demanding class of membrane proteins. Our service includes a thorough discussion and counselling of your project, so that the methods used provide optimal conditions to your protein of interest.

Our services... Show more »

Cube Biotech Services for protein crystallization are open to any project, but our focus lies in applications for the demanding class of membrane proteins. Our service includes a thorough discussion and counselling of your project, so that the methods used provide optimal conditions to your protein of interest.

Our services include, but are not limited to:

  • Initial de novo crystallization of soluble and membrane proteins
  • Reproduction of established crystallization conditions
  • Optimization of crystallization hits
  • Cocrystallization with ligands or drug candidates

For fast crystallization success, we employ a range of crystallization methods such as:

  • Classical vapor diffusion in sitting and hanging drop
  • Classical LCP batch methods
  • Controlled in meso phase (CIMP), a combination of LCP & vapor diffusion methods
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Cell-Free Protein Expression
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Cube Biotech Services for cell-free protein expression are suited for small- to large scale expression projects, as well as high-throughput expression screening experiments.

In combination with the nanodisc technology, even membrane proteins can be expressed in sufficient amounts for immunization, cryo electron microscopy, SPR... Show more »

Cube Biotech Services for cell-free protein expression are suited for small- to large scale expression projects, as well as high-throughput expression screening experiments.

In combination with the nanodisc technology, even membrane proteins can be expressed in sufficient amounts for immunization, cryo electron microscopy, SPR and interaction studies. We use E.coli cell-free lysates in dialysis mode which provide the highest yields and the most cost-effective solution. However, if other cell-free systems are preferred, we are open for discussions. All cell-free expression projects are non-GMP development projects.

Applications include:

  • Expression construct screening and screening of best expression conditions
  • Reconsitution of membrane proteins into nanodiscs
  • Small scale expression of proteins for interaction studies, or SPR
  • Large scale expression of proteins for immunization projects or Cryo electron microscopy
  • Labelling of proteins with biotin, fluorescent labels, or isotope-marked amino acids for NMR

Cell-free expression can be combined with a range of other services, including protein purification and SPR measurements. Our service offering includes a thorough discussion and counselling of your project, making sure that the methods used provide optimal conditions for the expression and purification of your protein of interest.

Any service package can be booked on its own, or in conjunction with others.

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Ligand Binding Assays
Price on request

Surface plasmon resonance (SPR) measurements are used to determine ligand binding kinetics. In connection with the nanodisc technology, even highly flexible proteins such as GPCRs can be immobilized and stabilized during the measurement. Alternatively or in addition, ELISA-type experiments can be performed.

Surface plasmon resonance (SPR) measurements are used to determine ligand binding kinetics. In connection with the nanodisc technology, even highly flexible proteins such as GPCRs can be immobilized and stabilized during the measurement. Alternatively or in addition, ELISA-type experiments can be performed.

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Protein Identification
Price on request

Cube Biotech offers a comprehensive set of protein characterization methods to ensure structural integrity and functionality of the proteins produced in our service offering. We always provide a thorough discussion and counselling of your project, so that the methods used provide optimal analysis data for your protein of interest.

Cube Biotech offers a comprehensive set of protein characterization methods to ensure structural integrity and functionality of the proteins produced in our service offering. We always provide a thorough discussion and counselling of your project, so that the methods used provide optimal analysis data for your protein of interest.

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Baculovirus Protein Expression
Price on request

Cube Biotech Services for protein expression are open to any small to medium-sized non-GMP projects, with particular expertise in the demanding class of membrane proteins. Available expression hosts are E.coli and insect baculovirus systems. Alternatively, proteins can be expressed in E.coli cell-free lysates.

Our service... Show more »

Cube Biotech Services for protein expression are open to any small to medium-sized non-GMP projects, with particular expertise in the demanding class of membrane proteins. Available expression hosts are E.coli and insect baculovirus systems. Alternatively, proteins can be expressed in E.coli cell-free lysates.

Our service offering includes a thorough discussion and counselling of your project, making sure that the methods used provide optimal conditions for the expression and purification of your protein of interest. Any service package can be booked on its own, or in conjunction with others.

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Bacterial Protein Expression
Price on request

Cube Biotech Services for protein expression are open to any small to medium-sized non-GMP projects, with particular expertise in the demanding class of membrane proteins. Available expression hosts are E.coli and insect baculovirus systems. Alternatively, proteins can be expressed in E.coli cell-free lysates.

Our service... Show more »

Cube Biotech Services for protein expression are open to any small to medium-sized non-GMP projects, with particular expertise in the demanding class of membrane proteins. Available expression hosts are E.coli and insect baculovirus systems. Alternatively, proteins can be expressed in E.coli cell-free lysates.

Our service offering includes a thorough discussion and counselling of your project, making sure that the methods used provide optimal conditions for the expression and purification of your protein of interest. Any service package can be booked on its own, or in conjunction with others.

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Protein Expression
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Protein Services
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Biology
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Protein Sequencing
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Protein Purification and Quantification
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Bioparticle Technology
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Biomolecular Interaction Analysis
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Biochemistry & Molecular Biology
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Structural Biology
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Liquid Chromatography
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Liquid Chromatography Services

Liquid Chromatography Services

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Chromatography
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Chromatography Services

Chromatography Services

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Purification Services
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Purification Services

Purification Services

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Analytical Chemistry Services
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Analytical Chemistry Services

Analytical Chemistry Services

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Chemistry and Materials
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Chemistry and Materials Services

Chemistry and Materials Services

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Ion Exchange Chromatography
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2018-03-06 00:41:17 -0800

Net Promoter Score of 8 received for Nanodisc Technology.

Additional Ratings: satisfaction with timeliness: 10.

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