Creative Diagnostics is a leading manufacturer and supplier of antibodies, viral antigens, innovative diagnostic components and critical assay reagents. We provide contract biologic R&D and manufacturing services to the diagnostic manufacturers along with GMP biologics manufacturing for the biopharmaceutical market. Our goal is to provide a trusted source for all your assay development and manufacturing needs.
We also assist clients in rapidly developing, manufacturing and commercializing point of care lateral flow, flow through and ELISA assays for any market segment. We develop using fluorescent, visual colloidal gold and paramagnetic labels, and high sensitivity, quantitative and reader-based tests are our specialty.
With the decades of experiences and our unique technologies, Creative Diagnostics prides itself on its commitment to providing its customers in research and industry the highest quality products and services.
Biomolecular Interaction Analysis, Biacore
This biosensor technology is based on the principle of surface plasmon resonance (SPR). This phenomenon occurs when polarized light, under conditions of total internal reflection, strikes an electrically conducting gold layer at the interface between media of different refractive index: the glass of a sensor surface (high refractive index) and a buffer (low refractive index). SPR is a powerful technique to detect the binding activity between all types of molecular interactions. This includes drugs and targets, antibodies and antigens or any pair of interacting molecules including protein-protein, protein-nucleic acid and protein-lipid interactions. Interactions are measured in real time allowing relative comparisons between different molecules or complete determination of kinetic parameters. Typically, a protein is bound to a 'chip' and analyte containing interacting components is examined. SPR does not require any type of labeling of the test molecules. Real time kinetics data is accomplished on the Unit BIAcore 3000 instrument. Quantitative information, such as kinetic parameters and equilibrium constants for complex formation can be obtained within a range of 100mM-1pM are analyzed with the BIAcore evaluation software. Biacore systems characterize molecules in terms of:
• specificity of their interactions
• on and off rates constants (kinetics)
• binding strength (affinity)
All routine procedures for preparation of chips, as well as immobilization, programming and initial running of the sample are performed by the CD staff.
Isothermal Titration Calorimetry (ITC)
ITC is a powerful analytical tool which measures the binding affinity and thermodynamics between any two biomolecules. Binding constants in the range of 103 to 108 M-1 can be accurately measured.
ITC is considered the "gold standard" assay for binding, and has many advantages:
• Universal assay - directly measures heat change associated with binding
• Label-free - uses native materials
• True in-solution technique
• Requires minimal assay development
• Has no molecular weight limitations
• Versatile, can be used with any biomolecule - proteins, nucleic acids, small molecule drugs, lipids, etc. Can be used with a wide range of biological buffers, ionic strengths, pH's In a single ITC experiment, one can determine:
• Binding affinity - Kd
• Directly measure sub-millimolar to nanomolar
• Can extend affinity range to picomolar using competitive ITC method
• Number of binding sites
• Multiple and different binding sites
• Enthalpy and entropy of binding
Differential Scanning Calorimetry (DSC)
DSC continuously measures heat capacity of a system as a function of temperature allowing for simultaneous determination of enthalpy, entropy and Gibbs energy for a thermal transition. This information has proven to be extremely important in the characterization of protein folding and oligonucleotide conformation. Differential Scanning Calorimetry (DSC) is a powerful analytical tool which directly measures the change in heat associated with the unfolding of a protein, lipid, or nucleic acid. In DSC, as the biomolecule is heated at a constant rate, a detectable heat change associated with thermal denaturation can be accurately measured. DSC experiments are:
• Label-free and use native materials
• True in-solution technique
• Easy to perform-require minimal assay development
• Conducted using a wide range of biological buffers, ionic strengths and pH's
• Universal assay-measures the heat change associated with denaturation
• Non-optical-unaffected by colored or turbid samples
• Versatile-can be used with proteins, nucleic acids, lipids and other biomolecules
In a single DSC experiment, one can determine:
• Transition midpoint (Tm)
• Enthalpy and heat capacity change associated with unfolding
Custom Antibody Labeling
Custom Antibody Fragmentation
Custom Antibody Purification
Custom Hybridoma Optimization
Custom Monoclonal Antibody Scale Up
Monoclonal Antibody Production
Polyclonal Antibody Production
Anti-idiotype Antibody Production
Anti-Hapten Antibody Production
Membrane Protein Antibody Production
Phospho Antibody Production
DNA Immunization Antibody Production
Quenchbody Generation Service
Custom Hybridoma Optimization
Creative Diagnostics offers custom hybridoma optimization service. Our scientists have special experience in this field. Although hybridomas are theoretically immortal and produce antibodies indefinitely, there are several limitations in antibody production using hybridomas.
We are experts in solving these problems through hybridoma optimization such as Re-Cloning and Re-Fusion. This way you recover the best existing clone in terms of stability and productivity.
Custom Hybridoma Optimization - Creative Diagnostics
Re-Cloning: If your hybridoma has diminished in levels of antibody production, or the monoclonality is in question, Creative Diagnostics will subclone hybridomas and establish new monoclonal cell lines selected for maximum antibody productivity.
Re-Fusion: If your hybridoma has diminished in levels of antibody production, or no longer grows well, Creative Diagnostics will re-fuse hybridomas to at least two different myeloma cell lines and establish new monoclonal cell lines for you.
We will make our best effort to optimize the hybridomas you request and ensure the secreted antibodies remaining the same. If you have any demand in this service, just inform us and in most cases we can accommodate your request.
Chromatin Immunoprecipitation (ChIP) is a tool that allowed us to determine the location of DNA binding sites on the genome for a given protein of interest. In some cases, it is usually more of a qualitative than a quantitative approach. ChIP also aims to determine the specific location in the genome that various histone modifications are associated with, indicating the target of the histone modifiers.
Creative Diagnostics can serve both standard ChIP assay and modified ChIP procedure to meet your research demands. Besides, we provide full line of downstream analysis for most methods, including PCR/qPCR-based assays, amplification, cloning, sequencing, library preparation and microarray analysis.
(1) N-ChIP: Native chromatin is used as the substrate, which means that proteins are not cross-linked to the DNA. Fragmentation of the chromatin is achieved by micrococcal nuclease digestion, resulting in a nucleosome based resolution. N-ChIP is restricted to proteins that are very tightly associated with chromatin, typically limiting this type of ChIP to histones and their modifications
(2) X-ChIP: Cross-linking is typically achieved using formaldehyde and chromatin is fragmented by sonication, creating random fragments. As the proteins are cross-linked to the DNA a broad range of chromatin associated factors can be analyzed using this technique.
(3) Biotinylation-ChIP: This modified ChIP procedure is to solve the problem that the cross-linking is never complete. The system is based on co-expression of the target protein fused to a short biotin acceptor domain together with the biotinylating enzyme BirA from Escherichia coli. The superior strength of the biotin–avidin interaction allows one to employ more stringent washing conditions in the ChIP protocol, resulting in a better signal/noise ratio.
Prepare chromatin samples.
Perform ChIP with a ChIP-qualified antibody.
Analyze & deliver the data.
(Service 3 and 4 are optional)
Epitope mapping has become more and more vital in both vaccine and antibody drug development. Knowledge on epitope of an antibody will largely facilitate your drug design and patent application. Creative Diagnostics is now able to offer a comprehensive and modular antibody epitope and seromarker profiling service workflow. It ranges from high throughput screening, using thousands of custom peptides, and parallel verification of the peptide hits for hundreds of samples to the validation of results by robust custom peptide ELISA. The assay modules can be combined or utilized individually according to your requirements.
Just send your sample to CD together with the sample submission form (see link below) and our experienced scientists will work with you on the optimal screening strategy for your project and help to design the peptide sequences. We will then produce tailored CDStarTM peptide microarrays and/or custom peptide ELISA plates specifically optimized for your requirements and perform screening and control experiments using your samples. The peptide screening platform is compatible with antibodies, sera, whole blood and any other biological fluids that contain antibodies. Subsequent to the assay, CD's biologists and computer scientists will perform data evaluation and analysis. Experiments and results are presented to you as a detailed report.
This is a comprehensive epitope mapping service. Just send us your protein/antibody sample and we’ll perform all the necessary functions from sample assay to data analysis. Our Epitope Mapping comprehensive service includes: assistance with your sequence designs, synthesis of a custom epitope mapping – peptide microarray, all on-chip binding reactions, detection of the bound antibody using standard immunological detection techniques, array image scan, and data analysis. We offer additional services for in-depth binding data analysis and curve analysis of quantitative measurements.
High throughput microarray format
Epitope mapping on a peptide microarray offers the opportunity to study thousands (up to tens of thousands of peptides/chip) of specific binding sequences in a single experiment. One experiment using our 4K epitope mapping – peptide microarray is equivalent to 41 experiments using a 96-well plate. A 30K microarray format is also available.
Custom microarray content
Peptides are synthesized on-chip, not pre-synthesized and spotted. There is no up-front cost to pre-synthesize a peptide library. We can synthesize a completely custom one-of-a-kind peptide microarray specifically for your experiment delivering results that cannot be achieved with an off-the-shelf assay. With the ability to program new sequence designs on the fly, you can quickly revise microarray design and content to keep experiments moving forward based on previous results.
Microfluidic epitope mapping – peptide microarrays using our CDStar™ technology produce more uniform spots and enhanced binding kinetics to deliver a more reliable reading of the assay signals. Well-designed positive and negative controls as well as multiple replicates of each peptide sequence contribute to data confidence.
Parallel verification of the peptide hits for hundreds of samples by robust custom peptide ELISA was also performed to validate the epitope mapping results.
Creative Diagnostics provides contract ELISA development kit services for the R&D and IVD community. We conduct ELISA kit development services for supporting regulatory approval submission. Creative Diagnostics will carry out the approval proposal and deliver the expected results and documents in a time and cost effective manner.
Our staff scientists follow rigorous guidelines for quality control with a focus on thorough optimization and validation of every aspect of assay development, including antibody specificity, assay sensitivity, reproducibility (intra- and inter-assay), cross-reactivity, standard curve range, assay accuracy (linearity and recovery) and kit stability.
Through combined expertise in antibody generation and immunoassay development, we have produced a large number of diagnostic antibodies that cover a wide range of disease portfolio including infectious diseases cardiovascular diseases, cancer and bone diseases, with osteoporosis in particular.
Immunoassay Service Formats:
The direct ELISA uses the method of directly labeling the antibody itself. Microwell plates are coated with a sample containing the target antigen, and the binding of labeled antibody is quantitated by a colorimetric, chemiluminescent, or fluorescent end-point. Since the secondary antibody step is omitted, the direct ELISA is relatively quick, and avoids potential problems of cross-reactivity of the secondary antibody with components in the antigen sample. However, the direct ELISA requires the labeling of every antibody to be used, which can be a time-consuming and expensive proposition. In addition, certain antibodies may be unsuitable for direct labeling. Direct methods also lack the additional signal amplification that can be achieved with the use of a secondary antibody.
The sandwich ELISA measures the amount of antigen between two layers of antibodies. The antigens to be measured must contain at least two antigenic sites, capable of binding to the antibody, since at least two antibodies act in the sandwich. For this reason, sandwich assays are restricted to the quantitation of multivalent antigens such as proteins or polysaccharides. Sandwich ELISAs for quantitation of antigens are especially valuable when the concentration of antigens is low and/or they are contained in high concentrations of contaminating protein. Creative Diagnostics has successfully applied our proprietary technologies which can expedite development of ELISA with certain throughput and low cost. The ELISA kits are good enough to reach detection sensitivity at sub-femtogram per ml level and are useful for screening protein targets and quantifying their expression in different conditions.
This type of ELISA is frequently used for the detection of small analyte antigens containing a single epitope. Typically the plate is coated with antibody specific for the single epitope on the analyte. Next, free analyte and analyte ligated to a detection enzyme are incubated on the coated plate. The quantity of the enzyme-ligand conjugate bound to the plate is detected after incubation with an appropriate substrate and the resulting signal measured with a microplate reader. In this type of ELISA, there is an inverse relationship between the signal obtained and the concentration of the analyte in the sample, due to the competition between the free analyte and the ligand-enzyme conjugate for the antibody coating the microplate, i.e. the more analyte the lower the signal.
Immuno-Capture Enzymatic Assay
Fluorescence intensity or polarization
Fluorescence resonance energy transfer
Creative Diagnostics staff scientists have extensive antibody purification experience. We have developed robust proprietary purification procedures for antibodies from serum, ascites fluid, yolks and culture supernatants for use in early discovery research, preclinical trials and in vitro medical devices. All purified antibodies can be analyzed by electrophoresis, ELISA, western blot and HPLC to determine purity and integrity. Corresponding test data will be provided to the customer.
Creative Diagnostics owns innovative technology that enables direct and quick labeling of antibodies for use in R&D applications, drug discovery and the development of diagnostic kits. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. As well as offering standard protocols for effective conjugation, we will also work with you to adapt and optimize the methods in order to provide you with a conjugated antibody suitable for your particular application needs.
Creative Diagnostics has a broad range of labels you can select to conjugate to your antibody or protein for use in a wide range of scientific applications (WB, IF, IHC, FC, ELISA, FRET and more).
Creative Diagnostics has a broad range of labels you can select to conjugate to your antibody or protein for use in a wide range of scientific applications (WB, IF, IHC, FC, ELISA, FRET and more). Please find below the list of popular choices and contact us for more options.
Fluorophores: FITC, TRITC, DyLight Fluors, Green fluorescent protein (GFP), R-Phycoerythrin, AMCA dyes, etc.
Fluorescent labels are generally used for detection of a protein or other labeled molecule via a fluorescence microscope, flow cytometer or some other fluorescence reading instrument. These can be useful in localization of a target within a cell, flow cytometry (FACS) analysis, western blot assays, and other immunoanalytical methods.
Nanoparticle: Nanogold, Colloidal Gold, Nanocrystal, etc.
We offer nanoparticle labeling to antibodies of your choice with a wide range of particle sizes and types for the performance of your conjugate in specific applications, while providing fast turnaround times. Besides, we can work to meet any of your specific needs and provide you with excellent quality using reagents either provided by you or sourced by us.
Biotin: Avidin, Biotin, Streptavidin, etc.
Antibodies labeled with biotin provide the user with a tool for increasing the sensitivity of an assay by its ability to amplify a given reaction. We can help you attach biotin to proteins and other macromolecules, for targeting specific functional groups or residues, including primary amines, sulfhydryls, carboxyls and carbohydrates.
Enzyme: HRP, AP, Beta--galactosidease, etc.
Enzymes can be covalently coupled to a specific antibody using maleimide-sulfhydryl coupling chemistries. Periodate oxidation methods are also used which specifically direct the coupling location to the Fc portion of the antibody molecule to maintain the activity of the antibody.
We offer custom antibody-oligonucleotide conjugates, which permit highly sensitive immunoassay such as immuno-PCR, by immobilizing analytes for each conjugate onto a solid support with cognate capture antibodies.
Antibody conjugated liposomes become more and more popular principally because of their potential use as targeted drug delivery systems and in diagnostic applications. Our company can help you save time and efforts by providing high quality antibody-liposome conjugates using the most advanced technology.
We will make our best effort to develop the conjugate you request. Whatever labels you want in your antibody, just inform us and in most cases we can accommodate your request.
Please feel free to contact us if you have any questions regarding our service.
Creative Diagnostics offers proprietary genetic immunization based polyclonal and monoclonal antibody generation services. This unique antibody development approach involves direct immunization of host animals with plasmid DNA encoding the target protein of interest. The immunized hosts then produce the encoded protein and raise antibodies. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein. Protein purification is not necessary for this genetic immunization approach, which can save several months in time over recombinant protein generation followed by antibody production.
In order for genetic immunization to be successful, the cDNA-encoded protein must be secreted by the transfected cells in immunized animals or expressed on the surface of the transfected cells in order to get stimulation of an antibody response.
The foremost advantage of this antibody production approach is its high success rate in generation of high-affinity antibodies recognizing difficult-to-express proteins in their native confirmation, such as GPCRs, ion channels and other multiple membrane spanning proteins. For these proteins, recombinant protein fragments or peptides derived from their extracellular domains may raise antibodies workable in Western blotting but are extremely hard to produce high-affinity antibodies that can recognize their integral proteins in their native form. This point is important in raising antibodies for diagnostic use, in which recognition of the antigens in their native form can be required. For therapeutic antibodies, targeting the antigens in the native conformation with a high-affinity is of course required!
Of note, our technology allows guaranteed antibody development against 7-membrane-spanning GPCR proteins!
High affinity antibodies can result from genetic immunization because of low level of expressed proteins and constant presentation to the immune system; these tend to favor development of high affinity antibodies.
Usually polyclonal antibody development via genetic immunization is tried first since it is an economical way to see whether the protein-encoding plasmid/cDNA will raise the desired antibodies, e.g. antibodies recognizing an integral antigen in its native 3D conformation. If this is successful, monoclonal development is followed.
The process of producing antibodies against a phospho-residue is more complicated than traditional antibody production using peptide immunogens. Phospho-specific antibodies are generated using peptides containing one or more phosphorylated amino acids. There are three residues which can be phosphorylated: Serine (S), Threonine (T) and Tyrosine (Y).
For phospho polyclonal antibody production:
To produce antiserum against Phospho-Peptides includes synthesis of phosphopeptides, conjugation and immunization of rabbits. In many cases, one would need to do affinity purification with a phospho-peptide column. Sometimes, one would also need to cross absorb the antibody with a non-phosphopeptide column in case there are some anti-non-phospho protein antibodies in the antiserum. In this case, synthesis of matching non-phospho peptides is required to make the negative-selection columns. Affinity purified, cross-absorbed polyclonal antibodies that are specific for the phosphor-peptides are usually required for downstream assays.
For phospho monoclonal antibody production:
In comparison with polyclonal antibody production, monoclonal antibody production against Phospho-Peptides is more straightforward; we just use the Phospho-Peptides to immunize the mice, and use Phospho-Peptides to screen for positive hybridoma clones. After that we use non-phospho-peptides to do negative selection. This negative selection is required [although widely forgotten] since peptide phosphorylation [or protein phosphorylation] is never 100% complete.
GPCRs represent a group of promising targets in science research and immune therapy. Unfortunately, they are multispan membrane proteins that are extremely difficult to generate antibodies using conventional approaches. The barriers are as followed.
Creative Diagnostics has developed proprietary methodologies MPATTM including conventional and novel approaches to solve these problems faced when generating antibodies against membrane proteins such as GPCRs. At least four feasible ways (Fig.1) access to you right now according to the structural property of membrane proteins. Our antibody platform also enables us to screen functional antibodies of membrane protein targets in vivo.
A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc.
Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response.
Small molecules, when used as haptens, are not immunogenic. However, on conjugating with carrier molecule they elicit antibody response. The production of anti-hapten antibodies of desired specificity largely depends on the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method. We design anti-hapten antibodies based on the HaptenDB information.
Both polyclonal and monoclonal production to haptens can be provided by Creative Diagnostics. We guarantee at least two (2) positive clones for ANTI-HAPTEN monoclonal antibodies development
Anti-idiotype Antibody Generation
Anti-idiotype antibodies (anti-IDs) are antibodies directed against the CDR regions (idiotype) of antibodies or T cell receptor (TCR) molecules. Due to the specificity of binding ‘drug’ antibody and receptors, anti-IDs are key reagents in pharmacokinetic (PK) studies and pharmacodynamic (PD) studies. Typically, anti-IDs are also deployed as positive controls in anti-drug antibody (ADA) assays . What is more, anti-IDs that against receptors, like TCRs, can specifically bind and/or block its ligand-binding site, which is instructive to the study of the structure and function of receptors.
What can Creative Diagnostics do for you?
Creative Diagnostics has extensive experience in developing highly specific, high affinity anti-IDs. We offer a comprehensive suite of anti-idiotype antibody generation services, concerning development of both polyclonal and monoclonal anti-IDs. Besides, we have a broad spectrum of development services available to support your study, such as Antibody Fragmentation, Antibody Labeling , contact assay development , etc.
Generation of polyclonal anti-idiotypic antibody
Generation of monoclonal anti-idiotype antibody
Anti-idiotype antibody Assay Development Services
Creative Diagnostics have accomplished more than 90% success rate of anti-IDs development for our clients. We develop custom antibodies that work in your hands and in the end applications. Please do not hesitate to contact us if you need free consultation and a detailed quotation of your project.
As a professional producer in this field, Creative Diagnostics provides customers with the most competitive products and the most thoughtful services across the world. Low cost of the antibody production is our priority advantage. We have been committed to advancing technologies and streamlining management. Another factor makes us special is that we are capable of meeting specific needs and requirements of customers. Horizontal and vertical cooperation established with different parties enable us to successfully accomplish whatever you are expecting in a most efficient way.
We are specialized in the following aspects:
» Bulk quantity polyclonal antibody production projects
» The most complete collection of hosts e g. Mouse, rat, goat, rabbit, chicken, sheep , donkey, Guinea pig, Llama, Camel, horse, dogs, bovine, pig and primates
» Custom phospho-specific polyclonal antibody service
» A portfolio of purified immunoglobulin e g. Protein A, Protein G, ammonium sulfate and peptide affinity purification
» Polyclonal packages : 28-days, 70-days and tailored ones
We provide rat and mouse monoclonal antibodies construction services. In particular, our proprietary mouse immunization approach allows us to provide mouse monoclonal antibodies within 70 days.
To generate monoclonal antibodies that fit your specific purposes, we tailor our protocols in every major step of antibody production, including antigen preparation [peptide synthesis or protein expression in E.coli, yeast, insect or mammalian cells], animal immunization and hybridoma screening.
Monoclonal Antibody Production: One-Stop Services
We express and purify your recombinant proteins with an appropriate E. coli, yeast, insect or mammalian cell expression system according to the specific purposes of the final antibodies.
Our proprietary chimeric protein expression method can produce a recombinant protein immunogen in a soluble form in bacterial cells that retains the specificity and immunogenicity of the original protein, and generate antibodies that bind to the protein surface. This approach highly increases the possibility of getting IHC-positive monoclonal antibodies, and antibodies that recognize the native protein and good for ELISA, IP, IHC, Immuno-fluorescence and Western Blotting.
We can design membrane mimc protein antigen (MMPA) using MPATTM platform. It remains structural characteristics such as multi-spanner extracellular domain and it is feasible to express in whole water-soluble form with high purity.
Alternatively, we perform peptide synthesis and conjugation.
We immunize animals with customized protocols that involve proper adjuvants, inoculation routes, dosage and timing. We can develop antibodies targeting impure native antigens or rare antigens of a minimum amount by adjusting the immunization strategies.
Our proprietary MagicTM adjuvant can elicit stronger and faster immune response within only 21 days.
We use different formats of immunogens for animal immunizations depending on the specific purpose. For antibodies intended for immunohistochemical [or immunocytochemical] staining, in addition to the natural immunogens, e.g. recombinant protein, we may alternatively use denatured antigens in animal immunization and hybridoma screening.
Our fast track mouse immunization protocol allows us to provide monoclonal antibodies within 70 days.
Development of Hybridoma
We fuse splenocytes with proper myeloma cells using optimized PEG mediated fusion protocols.
We screen hybridoma clones with customized protocols according to the final applications of the requested antibodies, including but not limited to immunoprecipitation, immunoblotting, various ELISA and particularly, IHC methods.
Goal-oriented method to screen IHC positive monoclonal antibodies. We introduce our proprietary IHC-positive hybridoma screening protocols to provide you IHC suited hybridoma clones.
Our revolutionary platform Omni-HybridomaTM platform ensures that a large number of hybridoma clones can be selected after each cell fusion. It can eliminate the possibility of overgrowth of potentially valuable slow-growing clones by fast-growing clones.
We deliver your positive hybridoma clones in culture or in cryopreservation as well as the derived monoclonal antibodies.
Ascites Production or in Vitro Antibody Manufacturing
Inoculation of hybridoma cells in to relevant animals to produce ascites. We normally use immuno-sufficient mice to produce ascites. Immuno-deficient mice are available for ascites production for non-murine hybridoma cell lines or murine cell lines of distinct genetic backgrounds. Optimized methods are used to generate tumors and accumulate ascitic fluid. Using nude or SCID mice in ascites production will eliminate the contamination from endogenous mouse IgG.
Cell culture in protein-free medium. We use stationary flasks or 5-50liter bioreactors to manufacture monoclonal antibodies. We have an antibody fermentation capacity of over 300L.
Immunohistochemistry (IHC) refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by using the principle of antibodies binding specifically to antigens in the tissue. In the most common instance, an antibody is conjugated to an enzyme, such as peroxidase, that can catalyze a color-producing reaction.
Creative Diagnostics has been providing high quality IHC service to our customers for years. We have established well-tested procedure to meet your research demands, which include different combinations of various tissue fixation processes, antigen retrieval, antibody titration options and eliminate background staining. Also, we have unparalleled bioinformatics support in performing IHC studies.
Creative Diagnostics offers a variety of IHC services:
Diagnostic IHC - For our veterinary diagnostic services, we offer a full complement of IHC markers to phenotype lymphomas and differentiate various soft tissue tumors.
Research IHC - We provide to screen the most appropriate antibody from our existing optimized antibodies. Besides, we are able to maximize the staining signals even for antigens with extremely low expression level using our proprietary technology.
Combination IHC and ISH - This service allow you to quickly visualize your target protein and transcript in its native setting.
Immunoprecipitation (IP) is an assay used to purify an antigen from a sample mixture or remove antigens from a complex mixture. For this purpose, purified specific antibody is covalently immobilized to beaded agarose or other affinity support by any one of several efficient conjugation chemistries for preparing an antibody affinity column.
Creative Diagnostics can provide following services:
Creative Diagnostics provides professional immunology experiment technical service with experienced technicians and perfect equipment. We provide customers with reliable, high-quality experimental data and the complete test report.
Welcome to discuss your IP experiment options with our expert team. We will help you to develop the optimal procedure to reach the best balance between yield, purity and the cost.
Creative Diagnostics is an evolving biotech company providing highly purified protein/ recombinant antigens worldwide. The protein/recombinant antigens are rigorously tested to meet the research and development demand for excellent quality, uncompromising biological activity at competitive prices.
Creative Diagnostics offers extensive expertise across expression systems for producing protein/recombinant antigens to support customs’ antibody generation. When you choose CD to develop protein/recombinant antigen for your antibody development, the end use application of your antibodies are considered during every phase of antigen production.
Bacterial expression systems (E. coli / Bacillus)
Creative Diagnostics provides rapid, high-level bacterial expression and purification of protein antigens to our customs. Our team utilizes both BacTEC™ protein soluble technology and FoldEZ™ protein refolding technology to achieve protein soluble expression and refolding. Besides, we generate OxiCyto™ competent E. coli strains to enable the correct formation of disulfide bonds in proteins that require them for biological activity.
Bacillus subtilis expression system, which is an ideal option for the expression of monomeric protein products, combined with our patented fermentation protocols allow us to offer fast process development and scale-up service to meet your antigen needs.
Yeast expression systems (P. pastoris / S. cerevisiae)
Yeast protein expression system is the most economical eukaryotic expression system for both secretion and intracellular expression. Our expertise in protein expression spans both P. pastoris and S. cerevisiae expression systems. Our YeastXceed™ Technology considers promoters, optimal copies of vector per cell, inducibility, signal peptides, and cellular location, which are essential for high-level recombinant protein production. Many secretory proteins that bacterial expression systems yield as insoluble or as inactive inclusion bodies can be produced successfully by our yeast expression system using native or yeast secretion signals.
Baculovirus-insect cell expression systems
BacuFlex™ Baculovirus/Insect Cell Expression Platform was developed by our in-house team of scientists for virus production and expression of proteins from baculovirus-infected insect cells in flexible scale. There are several advantages of insect cells over E. coli such as improved solubility, ability to incorporate post-translational modifications, and higher yields for secreted proteins.
Mammalian expression systems (CHO / 293)
Mammalian cells have the ability to perform most comprehensive post-translational modifications and to secrete glycoproteins that are correctly folded and contain complex antennary oligosaccharides with terminal sialic acid. Therefore, Mammalian antigen expression is the system of choice for many proteins that are difficult to produce and for antibody generation projects where detection of native antigen is required. Our Mammalian expression HostOptim™ systems enable generation of high yielding production systems across different industry relevant platforms (e.g. CHO, HEK, Pichia pastoris).
Cell-free in vitro Protein Expression
Site-specific incorporation of a biotinylated amino acid allows reduction of the required antigen material down to as little as 5-10 μg for an entire antibody generation project. Our team has developed cell-free expression systems in combination with site-specific biotinylation to meet the increasing demands for this purpose. Now, in only two hours, we can produce biotinylated or other modified proteins starting from plasmid or linear DNA.
Prokaryotic system: E.coli Lysates
Eukaryotic system: Rabbit Reticulocyte Lysates, Wheat Germ system.
As a leading supplier of highly purified protein/recombinant antigens, Creative Diagnostics is proud to offer the highest quality antigens supported by extensive research, development, and validation. We are committed to the highest standards of product performance, and our scientists are dedicated to the goal of accelerating your discovery.
The ImmunoFISH technique, combined conventional double immunofluorescence with a standard FISH technique, provides a unique opportunity to complement molecular and biochemical analyses by assessing specific interactions/associations of nucleic acid sequences and proteins in individual cells. The major challenge is, on the one hand to preserve the epitope detected by the antibody as well as the 3D architecture of the nucleus, and on the other hand, to allow the penetration of the DNA/RNA probe to detect gene loci or chromosome territories.
Creative Diagnostics offers well-established procedure for simultaneous detection of protein modifications by immunofluorescence and nucleic acid sequences by DNA/RNA FISH. Our experience in this field has enabled us to offer a full line of customized immune-FISH service.
Furthermore, we have developed a technical platform to generate mAbs that could be used specifically with stand DNA/RNA FISH, which allows the end user to quantify not only the number of nucleic acid molecules per cell (and location), but also quantify the amount of the target protein.
GAF antibody (green), FISH (red), DAPI for DNA (blue) and the merge of the three are shown
Our modified ImmunoFISH technique is easy to perform and to analyze, requiring a short time to learn. Comprehensive consultation from our experienced staff is also available upon request. We are eager to learn of our customers' specific ImmunoFISH requirement and to facilitate their research and product development.
Creative Diagnostics offers a wide variety of enzyme-linked immunosorbent assay (ELISA) kits, including HIV Ag-Ab, Syphilis screening Kits, Hormone assays, ToRCH (Toxoplasma, Rubella, CMV and HSV), infectious diseases, Allergy, diabetes, Tumour Markers and more. Our clients can easily access to our product portfolio. Our product range covers kits using the latest generation technology, providing the highest sensitivity on the market, effective with best value. Each of our kit goes through fit-for-purpose validation and stability testing to ensure high accuracy, sensitivity and specificity. In addition, we also provide Testing Services and Contract Research.
Should you need any assistance, our experienced scientists are more than happy to provide optimized protocols and helpful technical support to accelerate your research.
ELISA Matched Antibody Pair
ELISA Positive Controls
Autoimmune ELISA Kits
Cytokines and cytokine receptors ELISA Kits
Food Safety ELISA Kits
Infectious Disease ELISA Kits
Pesticides ELISA Kits
Veterinary ELISA Kits
Other ELISA Kits
Drugs & Chemicals
Immunoglobulin ELISA Kits
Phosphorylation ELISA kits
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